FastQCFastQC Report
Thu 26 May 2016
SRR937092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093008
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89290.42661088729713403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.35260257008095525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.22087827662388296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72700.037.054141
GTATCAA107550.033.175511
TATCAAC135400.026.3467062
TCAACGC135250.026.30384
ATCAACG135500.026.290953
CAACGCA137350.025.8670485
AACGCAG141100.025.2156546
GTACATG91150.023.142721
TATACCG1050.002197397922.6180255
TACATGG92500.022.5951922
GAGTACT88500.022.46487212-13
ACGCAGA159950.022.1252167
CGCAGAG162050.021.6358858
ACATGGG92400.021.1277963
GTACTTT93900.020.99388314-15
GCAGAGT171650.020.0383579
AGAGTAC138650.019.77075610-11
AGTACTT92050.019.63733912-13
CATGGGG52950.018.7479694
ACTTTTT110400.018.19955816-17
Produced by FastQC (version 0.11.5)