##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093008 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17287654896685 33.0 31.0 34.0 31.0 34.0 2 32.298213384755336 33.0 31.0 34.0 31.0 34.0 3 32.43104087514238 34.0 31.0 34.0 31.0 34.0 4 36.09641912501051 37.0 35.0 37.0 35.0 37.0 5 35.936088156375895 37.0 35.0 37.0 35.0 37.0 6 36.02502044903794 37.0 35.0 37.0 35.0 37.0 7 35.983112821355675 37.0 35.0 37.0 35.0 37.0 8 35.9222697667663 37.0 35.0 37.0 35.0 37.0 9 37.632466287754276 39.0 37.0 39.0 35.0 39.0 10-11 37.54569284971677 39.0 37.0 39.0 35.0 39.0 12-13 37.43795150329096 39.0 37.0 39.0 35.0 39.0 14-15 38.65166377768264 40.0 38.0 41.0 34.5 41.0 16-17 38.643951671469964 40.0 38.0 41.0 34.5 41.0 18-19 38.75130697063747 40.0 38.0 41.0 35.0 41.0 20-21 38.7440349009655 40.0 38.0 41.0 34.5 41.0 22-23 38.64924047113055 40.0 38.0 41.0 34.0 41.0 24-25 38.55095967143939 40.0 38.0 41.0 34.0 41.0 26-27 38.35031829787559 40.0 38.0 41.0 34.0 41.0 28-29 38.194295243974224 40.0 38.0 41.0 34.0 41.0 30-31 38.19909670674933 40.0 38.0 41.0 34.0 41.0 32-33 38.08327560143105 40.0 37.0 41.0 33.5 41.0 34-35 37.95669725103774 40.0 37.0 41.0 33.0 41.0 36-37 37.809258493039685 40.0 37.0 41.0 33.0 41.0 38-39 37.63733416212456 40.0 37.0 41.0 33.0 41.0 40-41 37.31508479661807 40.0 36.5 41.0 32.5 41.0 42-43 37.09847955669544 39.5 36.0 41.0 31.0 41.0 44-45 36.85761353993869 39.0 36.0 41.0 31.0 41.0 46-47 36.460282999396085 39.0 35.5 41.0 30.5 41.0 48-49 36.073968422480945 39.0 35.0 40.0 29.5 41.0 50-51 35.767197258682245 39.0 35.0 40.0 28.5 41.0 52-53 35.48555715028323 38.5 35.0 40.0 28.0 41.0 54-55 35.088409838853934 38.0 34.0 40.0 27.0 41.0 56-57 34.84487039705534 38.0 34.0 40.0 26.5 41.0 58-59 34.494431220520894 37.5 34.0 40.0 26.0 41.0 60-61 34.080983445834896 37.0 33.0 40.0 26.0 41.0 62-63 33.68305090090435 36.5 33.0 39.0 25.0 40.0 64-65 33.3357753529848 36.0 33.0 39.0 24.5 40.0 66-67 32.985405932514354 35.5 32.0 39.0 23.5 40.0 68-69 32.519621043015604 35.0 32.0 38.0 22.0 40.0 70-71 32.06547347167331 35.0 31.0 37.5 21.5 39.5 72-73 31.649386672196187 35.0 31.0 37.0 20.0 39.0 74-75 31.167099217967632 34.0 30.5 36.0 19.0 39.0 76-77 29.772842722053618 33.0 28.5 35.0 18.0 37.0 78-79 30.44227494591516 34.0 30.0 35.0 18.0 37.0 80-81 30.31615263773478 34.0 30.0 35.0 17.5 37.0 82-83 29.985531827876436 34.0 30.0 35.0 13.0 36.5 84-85 29.56698636603396 34.0 29.5 35.0 7.5 36.0 86-87 29.19273552705006 34.0 29.0 35.0 6.5 35.5 88-89 28.820701593113835 34.0 29.0 35.0 2.0 35.0 90-91 28.372045639577106 33.0 29.0 35.0 2.0 35.0 92-93 27.87708169295101 33.0 27.0 35.0 2.0 35.0 94-95 27.316977527080642 33.0 26.5 34.0 2.0 35.0 96-97 26.539174241092248 32.0 25.0 34.0 2.0 35.0 98-99 25.65104337871618 32.0 24.0 34.0 2.0 35.0 100-101 22.46099752127082 28.0 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12325617774575903 1101 2 0.20091212181824858 1101 3 0.17590667538743787 1101 4 0.11877122397704909 1101 5 -0.04108921541789812 1101 6 0.07835789942781446 1101 7 0.0942252163331716 1101 8 0.15855236532146222 1101 9 0.11546794109499814 1101 10-11 0.2115179762720274 1101 12-13 0.17061792095287132 1101 14-15 0.29522049142695295 1101 16-17 0.2547098790654161 1101 18-19 0.07416742214105199 1101 20-21 0.04966651719081483 1101 22-23 0.1300845038901457 1101 24-25 0.14555344425706096 1101 26-27 0.2196530872950504 1101 28-29 0.30904147259224146 1101 30-31 0.32070397574093334 1101 32-33 0.31723561564993474 1101 34-35 0.11737217529989863 1101 36-37 0.11562639788940032 1101 38-39 0.17183275531488817 1101 40-41 0.29303625422229373 1101 42-43 0.35300751192698243 1101 44-45 0.3093035992038651 1101 46-47 0.331766158678235 1101 48-49 0.3655128918256665 1101 50-51 0.48235091591566004 1101 52-53 0.6229131546757287 1101 54-55 0.3782400151438452 1101 56-57 0.4067795247444863 1101 58-59 0.3995647515233216 1101 60-61 0.4502132479793062 1101 62-63 0.4327960132255342 1101 64-65 0.5625113593336053 1101 66-67 0.4730688472059015 1101 68-69 0.21540370470501813 1101 70-71 0.06574775487103324 1101 72-73 0.25912189147983966 1101 74-75 0.08200632263982044 1101 76-77 0.1102457830357757 1101 78-79 0.26643950542311856 1101 80-81 0.24434941768402396 1101 82-83 0.3979383015056648 1101 84-85 0.2501231908828885 1101 86-87 -0.05075985661478555 1101 88-89 0.13226915759307545 1101 90-91 0.3564339313936067 1101 92-93 0.1127917206117921 1101 94-95 0.09832395093102875 1101 96-97 -0.07953636972958833 1101 98-99 0.04768673850094629 1101 100-101 0.3012418111757995 1102 1 -0.12404803319481772 1102 2 0.053168713711201576 1102 3 0.01729982768968341 1102 4 0.10023364034855575 1102 5 -0.12561577976264005 1102 6 0.006014727448281576 1102 7 0.09674076139417309 1102 8 0.12209585213768293 1102 9 0.1031956073681215 1102 10-11 0.2303113647017767 1102 12-13 0.12103389891430538 1102 14-15 0.2366156114584399 1102 16-17 0.15727973345344992 1102 18-19 0.1499285398112633 1102 20-21 0.19794278637618135 1102 22-23 0.05663507453162708 1102 24-25 0.04821696255693553 1102 26-27 0.13910133601603292 1102 28-29 0.054780158149107194 1102 30-31 0.06679409774015 1102 32-33 -0.09804734381484081 1102 34-35 0.2383953945049342 1102 36-37 0.061803099975186626 1102 38-39 0.26672492375251267 1102 40-41 0.25811771821285134 1102 42-43 0.21077571342245704 1102 44-45 0.23167943548879322 1102 46-47 0.1002415620627275 1102 48-49 0.2150300110700556 1102 50-51 0.2659943633772883 1102 52-53 0.29912960489217966 1102 54-55 0.4355757097522712 1102 56-57 0.3045178167554994 1102 58-59 0.15490123322344118 1102 60-61 0.26458121334727025 1102 62-63 0.05642293176154567 1102 64-65 0.14675024166340478 1102 66-67 0.19132268427792098 1102 68-69 0.23878150871919246 1102 70-71 -0.06175598381452119 1102 72-73 -0.2246474906531759 1102 74-75 -0.027064122066988716 1102 76-77 0.013490568553290672 1102 78-79 -0.3898725764344171 1102 80-81 -0.01489300655839898 1102 82-83 0.10189265018728477 1102 84-85 -0.1550983754295885 1102 86-87 -0.18306760946799017 1102 88-89 -0.26063481721998727 1102 90-91 -0.19624414810265378 1102 92-93 -0.26844283271367075 1102 94-95 -0.2846544040473269 1102 96-97 -0.21647694430652464 1102 98-99 -0.2277973228922079 1102 100-101 -0.11176409201192072 1103 1 0.04798424156011549 1103 2 0.15541731882090914 1103 3 0.14252741505386268 1103 4 0.08428294744816611 1103 5 0.025982575735113755 1103 6 0.035907331383334906 1103 7 0.0720096011530913 1103 8 0.13701453597653312 1103 9 0.15784454249698143 1103 10-11 0.15433354310790293 1103 12-13 0.16741487526758903 1103 14-15 0.2193249149222396 1103 16-17 0.046548167680349195 1103 18-19 0.08980387367598297 1103 20-21 0.0821574526512947 1103 22-23 0.11479081280965886 1103 24-25 0.07336973625609744 1103 26-27 0.12449379312052855 1103 28-29 0.07833177957797943 1103 30-31 0.19685940237859967 1103 32-33 0.27438137344543634 1103 34-35 0.03916695412512894 1103 36-37 -0.008166205446350716 1103 38-39 0.019154969479743045 1103 40-41 0.1964394449402036 1103 42-43 0.13483564825765626 1103 44-45 0.08556030696311012 1103 46-47 0.035802175115264845 1103 48-49 0.06431298851408229 1103 50-51 -0.1758516463275086 1103 52-53 -0.2898951605285234 1103 54-55 -0.08493861671499303 1103 56-57 -0.20425600825043944 1103 58-59 -0.1760891039274881 1103 60-61 -0.28650708076133213 1103 62-63 -0.015765263061631174 1103 64-65 -0.0746817757884557 1103 66-67 0.012386226949679724 1103 68-69 -0.04494896631262435 1103 70-71 0.03874533766049382 1103 72-73 -0.07402555836304003 1103 74-75 -0.15638163965169838 1103 76-77 0.029309113951899946 1103 78-79 -0.03444229298152379 1103 80-81 -0.10934770583543596 1103 82-83 0.04346912094003841 1103 84-85 -0.22234502279852464 1103 86-87 -0.455771459225371 1103 88-89 -0.14319204617777004 1103 90-91 -0.41096635867165787 1103 92-93 -0.36262379787986987 1103 94-95 -0.2611541733135958 1103 96-97 -0.13156972723502847 1103 98-99 -0.30163281673231523 1103 100-101 0.15961478676197416 1104 1 0.07318545638790397 1104 2 0.0872412118701078 1104 3 0.01667989556659677 1104 4 -0.05662905890837777 1104 5 -0.03024150207513543 1104 6 0.09083126104969352 1104 7 0.03979428752600711 1104 8 0.05708324698105827 1104 9 0.07157166575318286 1104 10-11 0.1924345250697641 1104 12-13 0.18442838771382952 1104 14-15 0.2596995107574571 1104 16-17 0.15932091159724138 1104 18-19 0.201279161886454 1104 20-21 0.1455269603779925 1104 22-23 0.09912740818057841 1104 24-25 0.12137051167808721 1104 26-27 0.07175492605035316 1104 28-29 0.09486514067333474 1104 30-31 0.06574876593423795 1104 32-33 0.04382496029868577 1104 34-35 0.09838113333855603 1104 36-37 -0.0279568175750029 1104 38-39 0.03881813956760283 1104 40-41 0.13336251541984012 1104 42-43 0.006465314850132131 1104 44-45 0.03127059590354264 1104 46-47 -0.04485996107659673 1104 48-49 0.07092920488553744 1104 50-51 0.03884690837205085 1104 52-53 -0.16699915084053174 1104 54-55 -0.043900484620415625 1104 56-57 -0.16706866007400123 1104 58-59 -0.31912454597785 1104 60-61 -0.06152461152093025 1104 62-63 -0.08850714566177231 1104 64-65 -0.09337925829958493 1104 66-67 -0.36217490574082234 1104 68-69 -0.12379101476692966 1104 70-71 -0.10774210840100551 1104 72-73 -0.11690262525754491 1104 74-75 -0.10959575185334458 1104 76-77 -0.0928093796233469 1104 78-79 0.09454700188704734 1104 80-81 -0.16770150169362807 1104 82-83 -0.008149392978069159 1104 84-85 0.08038816023696782 1104 86-87 0.04501383504009837 1104 88-89 0.05458693222768218 1104 90-91 0.1662500558631983 1104 92-93 -0.005856087026117507 1104 94-95 0.08772140496214575 1104 96-97 0.4098573124957774 1104 98-99 0.0759375635834516 1104 100-101 0.8458019243299724 1105 1 0.001222361672205352 1105 2 0.06228446250185016 1105 3 0.024089485954355894 1105 4 0.03918186852014571 1105 5 0.03858242420076152 1105 6 0.1382596047864766 1105 7 0.19353812417588045 1105 8 0.1620890317455519 1105 9 0.11192739334253332 1105 10-11 0.07625154733672446 1105 12-13 0.0613002099662836 1105 14-15 0.08632206093911776 1105 16-17 -0.029916664369075363 1105 18-19 0.009845368918341535 1105 20-21 0.15578834238548467 1105 22-23 0.05770791800362929 1105 24-25 -0.015172267117662841 1105 26-27 0.05589023645935498 1105 28-29 0.043117498111975294 1105 30-31 0.16288237129680994 1105 32-33 0.10051553220137066 1105 34-35 0.13398571628430034 1105 36-37 0.1571318681365952 1105 38-39 0.053629034091301264 1105 40-41 0.15544597694989903 1105 42-43 0.23792350542576202 1105 44-45 0.22916862600929022 1105 46-47 0.12086903813872851 1105 48-49 0.1721898440125429 1105 50-51 0.16701636966720912 1105 52-53 0.20107699525016898 1105 54-55 0.1349006172349121 1105 56-57 3.797964374641083E-4 1105 58-59 0.07272245474725736 1105 60-61 -0.13252085676804626 1105 62-63 -0.08235310255933825 1105 64-65 -0.031149267015756266 1105 66-67 0.011567355320302397 1105 68-69 0.13946512672670508 1105 70-71 -0.05593033745606846 1105 72-73 -0.077591205577626 1105 74-75 0.0604347727768797 1105 76-77 -0.11371511258074563 1105 78-79 0.20615296635512337 1105 80-81 0.09141343534406943 1105 82-83 0.09940699616641169 1105 84-85 -0.2650456226435409 1105 86-87 -0.1879486104657886 1105 88-89 -0.02681756583801942 1105 90-91 -0.0826398866206084 1105 92-93 -0.02870152345098731 1105 94-95 0.04745322342454017 1105 96-97 0.12497693594928094 1105 98-99 0.07652888972720007 1105 100-101 0.340624097763385 1106 1 -0.04159579550017156 1106 2 0.037420449155895597 1106 3 0.06207823186677075 1106 4 0.11570441874348347 1106 5 -0.01820713051902345 1106 6 0.12287709893750076 1106 7 0.0896158417495414 1106 8 0.24891951430753068 1106 9 0.09625382858934017 1106 10-11 0.22916095332854525 1106 12-13 0.2308253449409321 1106 14-15 0.3392666448631516 1106 16-17 0.18756249185087626 1106 18-19 0.16129263865183674 1106 20-21 0.15381393105283792 1106 22-23 0.1616239288375212 1106 24-25 0.1373219309466407 1106 26-27 0.2238712768652249 1106 28-29 0.29153898105281684 1106 30-31 0.2305390278367625 1106 32-33 -0.006015590148550132 1106 34-35 0.03624765162361854 1106 36-37 -0.06666848312959672 1106 38-39 -0.036718101401611136 1106 40-41 0.03503225710021951 1106 42-43 0.00364859701238629 1106 44-45 -0.03235962993781527 1106 46-47 -0.05555997983537253 1106 48-49 -0.3361857251701039 1106 50-51 -0.0633352300592378 1106 52-53 -0.01730983144093301 1106 54-55 0.11884491295076316 1106 56-57 0.08711677525744932 1106 58-59 0.0166916201778875 1106 60-61 -0.31964450776988684 1106 62-63 -0.171795560841133 1106 64-65 -0.12947115991919844 1106 66-67 0.05876650489236823 1106 68-69 -0.10900496105563917 1106 70-71 0.014303088364250982 1106 72-73 0.04649695476760485 1106 74-75 -0.1264504151756185 1106 76-77 0.07242874463675619 1106 78-79 0.10942434300672588 1106 80-81 0.16724439996344742 1106 82-83 0.2959012615077974 1106 84-85 0.21326450247187623 1106 86-87 0.12228440485029424 1106 88-89 0.32928815724666194 1106 90-91 0.1791466449065453 1106 92-93 0.3990463106403048 1106 94-95 0.6255455247312263 1106 96-97 0.5997571522679088 1106 98-99 0.615852059469848 1106 100-101 0.9979280370931392 1107 1 0.2440552701892429 1107 2 0.32050991933353856 1107 3 0.27850151227629283 1107 4 0.16005663389930191 1107 5 0.1558607306267632 1107 6 0.12443233012978538 1107 7 0.13719004851822802 1107 8 0.18037976356722396 1107 9 0.15905876591999402 1107 10-11 0.23645928946203298 1107 12-13 0.26803725079985696 1107 14-15 0.36353238097068896 1107 16-17 0.3980313145541956 1107 18-19 0.37983367376663324 1107 20-21 0.24434070993589785 1107 22-23 0.2763073532855742 1107 24-25 0.40159694056319495 1107 26-27 0.4039802503508625 1107 28-29 0.40150078473080697 1107 30-31 0.2037326689148884 1107 32-33 0.24668184015949635 1107 34-35 0.5192459474700044 1107 36-37 0.5337847222301733 1107 38-39 0.49167714257589523 1107 40-41 0.5695967257089691 1107 42-43 0.3378187789090745 1107 44-45 0.3460439218291427 1107 46-47 0.3335313560994422 1107 48-49 0.35434131383944134 1107 50-51 0.3013843682274455 1107 52-53 0.428428109989639 1107 54-55 0.543231022586923 1107 56-57 0.2614190753933272 1107 58-59 0.270289413264841 1107 60-61 0.35739854999086873 1107 62-63 0.36762725751872694 1107 64-65 0.365770151287343 1107 66-67 0.4339085583373006 1107 68-69 0.3273880991600677 1107 70-71 0.21673660410043993 1107 72-73 0.4305945126026671 1107 74-75 0.49139045700245276 1107 76-77 0.2465790333608915 1107 78-79 0.5236359305358711 1107 80-81 0.2875646026540011 1107 82-83 0.5195106985062985 1107 84-85 0.5605748109266067 1107 86-87 0.7293778263491646 1107 88-89 0.6131788169826926 1107 90-91 0.3837995272542649 1107 92-93 0.5404253776771739 1107 94-95 0.8397658590132622 1107 96-97 0.6819390736726412 1107 98-99 1.00040857796305 1107 100-101 1.7732667136411457 1108 1 0.06669211565683497 1108 2 0.10828491845071397 1108 3 0.04588299740795776 1108 4 0.04885699088049478 1108 5 -0.051415971534709115 1108 6 0.014936694481747281 1108 7 -0.0063504216568261995 1108 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-0.8504438027966117 2316 60-61 -0.6000952778262274 2316 62-63 -0.4519139953287805 2316 64-65 -0.6312434168283474 2316 66-67 -0.6919679363697995 2316 68-69 -0.6150051917877093 2316 70-71 -0.4824911957990565 2316 72-73 -0.566845031816424 2316 74-75 -0.39730533974968907 2316 76-77 -0.50511207268136 2316 78-79 -0.6219203463020015 2316 80-81 -0.7786246342121821 2316 82-83 -0.8237480400968415 2316 84-85 -0.7565024150481072 2316 86-87 -0.7381673273875258 2316 88-89 -1.216900503085565 2316 90-91 -1.2124169382955863 2316 92-93 -1.1508193429753177 2316 94-95 -1.1961288577373494 2316 96-97 -0.8533835325468111 2316 98-99 -1.1258547499507259 2316 100-101 -1.7792817132040106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 22.0 5 52.0 6 84.0 7 221.0 8 546.0 9 1078.0 10 1820.0 11 3101.0 12 4688.0 13 6447.0 14 8467.0 15 11694.0 16 15556.0 17 21577.0 18 19406.0 19 12345.0 20 11278.0 21 11623.0 22 12123.0 23 13421.0 24 15465.0 25 18238.0 26 22101.0 27 27496.0 28 34266.0 29 43015.0 30 53678.0 31 67559.0 32 87232.0 33 114762.0 34 158118.0 35 226486.0 36 347419.0 37 465461.0 38 246151.0 39 10004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74717155512738 18.808495346177757 14.973470560996205 22.47086253769866 2 16.146581388926318 20.37434672797796 39.04294752896185 24.436124354133877 3 19.90598202392351 24.286718993370297 29.40433940881145 26.402959573894748 4 13.904246902066308 16.828937108697147 35.715200324126805 33.551615665109736 5 14.655602841352408 36.92181407364831 33.05544765347618 15.36713543152311 6 32.693807190416855 35.29260279941596 16.929510063984466 15.084079946182719 7 29.140468227424748 32.486048733874824 20.03554706163402 18.33793597706641 8 27.18068922813482 34.167523487726754 19.105373701390533 19.546413582747892 9 26.917600809553342 16.629861548414098 19.77347413242007 36.67906350961249 10-11 25.525153272228295 26.344094241398025 26.406349139611507 21.724403346762173 12-13 26.570388435335797 23.553509124842677 27.26052125848959 22.61558118133194 14-15 23.561972404681956 24.787241794089855 25.689924758502148 25.960861042726037 16-17 22.836224228478823 28.04329940449344 26.14653169027543 22.97394467675231 18-19 22.871055238015153 26.357801755090904 28.7109823855457 22.06016062134824 20-21 23.63442903904416 25.443134197074137 28.85965955865033 22.06277720523137 22-23 23.473703196276126 25.210196454103173 28.926926049310016 22.38917430031068 24-25 23.206924427949215 25.466986081196524 28.923753318209876 22.402336172644382 26-27 23.0529420870672 25.561847946778887 28.960909773703253 22.424300192450662 28-29 22.884377091041046 25.746121667562903 28.924014330581393 22.445486910814658 30-31 22.977873461483885 25.56064074319866 28.81488212891787 22.646603666399585 32-33 23.08688299523484 25.60570223332057 28.84800295460425 22.459411816840337 34-35 23.308964896455244 25.654512548447016 28.626670323285914 22.409852231811822 36-37 23.34705941233398 25.534233694677123 28.6861115603654 22.4325953326235 38-39 23.079499683318165 25.60671867209131 28.694599493643548 22.619182150946976 40-41 23.323123863618175 25.590715490244463 28.458452889082448 22.627707757054914 42-43 23.096519459075168 25.718105234189263 28.27889334393371 22.906481962801863 44-45 23.249260506634776 25.717455483478872 28.285340520509685 22.747943489376667 46-47 23.58781653129479 26.072169135212615 27.718299092212135 22.62171524128046 48-49 23.732701004704005 26.229088375108606 27.27741388038105 22.76079673980634 50-51 23.470466440814985 26.505154286989303 27.313289534256757 22.71108973793895 52-53 23.712523581587096 26.51553428464746 26.92434948025549 22.847592653509952 54-55 23.469690512410846 26.43207766047717 26.981860556672505 23.11637127043948 56-57 23.44400382226469 26.683516483516485 27.010821786908746 22.861657907310082 58-59 23.76952096649508 26.35986232242048 26.927658114692456 22.942958596391982 60-61 23.515501054601582 26.3256218852583 27.436483218468677 22.722393841671444 62-63 23.283929158416978 26.39101236115677 27.50663160389258 22.818426876533678 64-65 23.322903363438343 26.505143078833466 27.299335358171284 22.872618199556904 66-67 23.302329303645593 26.531390833525442 27.15310384697618 23.013176015852785 68-69 23.10385710883225 26.446132129132003 27.3268034890496 23.123207272986143 70-71 23.33903886650605 26.40454658928164 27.127313816667243 23.12910072754506 72-73 23.4533571651423 26.47170259354869 26.920770701972906 23.1541695393361 74-75 23.239845441336463 26.8451790243998 26.761372948029244 23.15360258623449 76-77 23.445250515287583 26.642207427976793 26.73828933122982 23.174252725505802 78-79 23.383411299458242 26.68125654860504 26.7060056770105 23.229326474926218 80-81 23.415393950687182 26.80742391774349 26.682362103522994 23.094820028046335 82-83 23.37971716762466 26.90103924665308 26.587088955528802 23.132154630193455 84-85 23.362944541396562 26.863684522977653 26.421845641872803 23.351525293752978 86-87 23.262674026433814 26.823876338329512 26.610498513856417 23.302951121380257 88-89 23.44508683776929 26.76305194403414 26.35418017926333 23.43768103893324 90-91 23.4387079080621 26.71270314355908 26.390172280836204 23.45841666754261 92-93 23.2887069710197 26.60919117366011 26.590748817013598 23.51135303830659 94-95 23.340886260560463 26.627544335125414 26.556904358918924 23.474665045395202 96-97 23.240464356827097 26.71606937879912 26.47586421898684 23.567602045386952 98-99 23.1164230163271 26.80248495873731 26.236429418643404 23.844662606292186 100-101 22.846088792905743 27.11521003745891 26.21507338888464 23.823627780750705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 446.0 1 346.5 2 262.0 3 286.0 4 268.0 5 274.0 6 455.5 7 919.0 8 1594.0 9 2096.0 10 2134.0 11 2086.0 12 2238.0 13 2431.0 14 2661.0 15 2912.0 16 3164.0 17 3354.5 18 3534.5 19 3718.5 20 4007.5 21 4474.0 22 5129.5 23 6005.5 24 7137.5 25 8489.0 26 10143.0 27 12203.0 28 14241.5 29 16498.5 30 19472.0 31 22666.5 32 25239.5 33 27676.0 34 31328.5 35 34259.0 36 37634.0 37 41914.0 38 44829.0 39 47169.0 40 50165.5 41 54945.0 42 58722.0 43 62509.0 44 68229.5 45 72759.5 46 76355.5 47 80728.0 48 85261.0 49 85219.0 50 82427.0 51 81620.0 52 81601.5 53 81470.0 54 80649.0 55 77762.5 56 72606.5 57 66492.0 58 61432.0 59 55399.5 60 46392.0 61 37572.0 62 30223.5 63 23981.0 64 17890.5 65 13160.0 66 9980.5 67 7533.0 68 5216.5 69 3302.0 70 2208.0 71 1349.0 72 846.5 73 572.0 74 386.5 75 228.0 76 140.5 77 77.0 78 40.5 79 27.5 80 19.5 81 9.0 82 7.5 83 6.0 84 3.0 85 3.5 86 2.0 87 1.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020401259813627087 2 0.003917806334232836 3 1.9111250410891882E-4 4 0.0 5 0.0029144656876610125 6 0.0 7 3.8222500821783763E-4 8 0.0 9 9.555625205445941E-5 10-11 0.0 12-13 0.007166718904084456 14-15 0.0038461391451919914 16-17 0.0 18-19 5.255593862995268E-4 20-21 9.077843945173644E-4 22-23 0.0012183422136943574 24-25 5.733375123267566E-4 26-27 1.9111250410891882E-4 28-29 7.166718904084457E-5 30-31 4.7778126027229704E-5 32-33 1.6722344109530398E-4 34-35 0.0 36-37 0.0016005672219121954 38-39 0.010200629906813544 40-41 7.883390794492902E-4 42-43 0.0 44-45 0.001003340646571824 46-47 3.8222500821783763E-4 48-49 1.6722344109530398E-4 50-51 0.001982792230130033 52-53 4.0611407123145255E-4 54-55 0.0 56-57 3.8222500821783763E-4 58-59 9.555625205445941E-5 60-61 0.001791679726021114 62-63 0.0 64-65 4.7778126027229704E-5 66-67 2.3889063013614852E-5 68-69 1.1944531506807427E-4 70-71 5.01670323285912E-4 72-73 3.3444688219060796E-4 74-75 0.003870028208205606 76-77 9.555625205445941E-5 78-79 2.3889063013614854E-4 80-81 0.0020544594191708775 82-83 7.166718904084457E-5 84-85 0.0024127953643751005 86-87 3.3444688219060796E-4 88-89 0.0025561297424567895 90-91 0.0014333437808168913 92-93 0.0 94-95 2.3889063013614852E-5 96-97 2.627796931497634E-4 98-99 7.405609534220605E-4 100-101 0.0022933500493070263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2093008.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.26506050547388 #Duplication Level Percentage of deduplicated Percentage of total 1 74.34408795084627 38.855982549752525 2 13.642801983961839 14.260837423119293 3 4.768068877725792 7.476102251658166 4 2.2040314371290703 4.6077534567006975 5 1.2431603045569348 3.2486924267835775 6 0.7922870039992863 2.4845356921033996 7 0.5533546012396079 2.0244778200339337 8 0.4031840861798518 1.685795252722732 9 0.299977925011861 1.411052796094628 >10 1.6098165165134817 15.120529087023552 >50 0.09896175251508793 3.549339017995296 >100 0.03824613567956022 3.5131904572568056 >500 0.001470127011853557 0.5139386492904416 >1k 3.6753175296338727E-4 0.46457134117321175 >5k 1.8376587648169363E-4 0.7832017782917006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8929 0.42661088729713403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7380 0.35260257008095525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4623 0.22087827662388296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.777812602722971E-5 0.0 0.0 0.0 0.0 3 4.777812602722971E-5 0.0 0.0 0.0 0.0 4 4.777812602722971E-5 0.0 0.0 0.0 0.0 5 4.777812602722971E-5 0.0 0.0 0.0 0.0 6 1.4333437808168912E-4 0.0 0.0 0.0 0.0 7 2.8666875616337824E-4 0.0 0.0 0.0 0.0 8 2.8666875616337824E-4 0.0 0.0 0.0 0.0 9 2.8666875616337824E-4 0.0 0.0 0.0 0.0 10-11 3.3444688219060796E-4 0.0 0.0 0.0 0.0 12-13 3.3444688219060796E-4 0.0 0.0 0.0 9.555625205445942E-5 14-15 3.3444688219060796E-4 0.0 0.0 4.777812602722971E-5 1.6722344109530398E-4 16-17 4.300031342450674E-4 0.0 0.0 4.777812602722971E-5 2.8666875616337824E-4 18-19 6.450047013676011E-4 0.0 0.0 4.777812602722971E-5 2.8666875616337824E-4 20-21 7.166718904084456E-4 0.0 0.0 1.1944531506807428E-4 3.105578191769931E-4 22-23 7.166718904084456E-4 0.0 0.0 3.3444688219060796E-4 3.583359452042228E-4 24-25 7.644500164356754E-4 0.0 0.0 4.7778126027229713E-4 3.822250082178377E-4 26-27 7.644500164356754E-4 0.0 0.0 7.644500164356754E-4 3.822250082178377E-4 28-29 0.001146675024653513 0.0 0.0 0.0015289000328713507 3.822250082178377E-4 30-31 0.0012422312767079724 0.0 0.0 0.002795020372592938 3.822250082178377E-4 32-33 0.0012661203397215872 0.0 0.0 0.004538921972586822 3.822250082178377E-4 34-35 0.0014333437808168913 0.0 0.0 0.006617270454771314 3.822250082178377E-4 36-37 0.0018155687890347289 0.0 0.0 0.009340623638323407 3.822250082178377E-4 38-39 0.002293350049307026 0.0 0.0 0.01462010656433229 3.822250082178377E-4 40-41 0.002341128175334256 0.0 0.0 0.019445697293082494 3.822250082178377E-4 42-43 0.002484462553415945 0.0 0.0 0.02431906614785992 3.822250082178377E-4 44-45 0.0026755750575248637 0.0 0.0 0.02804575997798384 3.822250082178377E-4 46-47 0.0030339110027290863 0.0 0.0 0.03387469135330587 3.822250082178377E-4 48-49 0.0030578000657427015 0.0 0.0 0.03838972426287907 4.300031342450674E-4 50-51 0.0030578000657427015 0.0 0.0 0.04280920092039782 4.300031342450674E-4 52-53 0.0030578000657427015 0.0 0.0 0.05224538081077569 5.01670323285912E-4 54-55 0.003105578191769931 0.0 0.0 0.06791660614770703 5.255593862995269E-4 56-57 0.003153356317797161 0.0 0.0 0.09414679733665614 5.255593862995269E-4 58-59 0.003153356317797161 0.0 0.0 0.13279930129268497 5.255593862995269E-4 60-61 0.0031772453808107757 0.0 0.0 0.17450960531445653 5.255593862995269E-4 62-63 0.0032011344438243904 0.0 0.0 0.20064424025135116 5.255593862995269E-4 64-65 0.0032011344438243904 0.0 0.0 0.2335394800210988 5.255593862995269E-4 66-67 0.0035355813260149983 0.0 0.0 0.29741883451950496 5.255593862995269E-4 68-69 0.0035355813260149983 0.0 0.0 0.3912072959109569 5.255593862995269E-4 70-71 0.003631137578069458 0.0 0.0 0.48160351035447546 5.494484493131417E-4 72-73 0.003798361019164762 0.0 0.0 0.5880770642061568 5.733375123267565E-4 74-75 0.003917806334232836 0.0 0.0 0.6902983648414149 5.733375123267565E-4 76-77 0.003917806334232836 0.0 0.0 0.7821279230657503 6.211156383539862E-4 78-79 0.003917806334232836 0.0 0.0 0.8630162904298502 6.211156383539862E-4 80-81 0.004443365720532363 0.0 0.0 0.9327962434926192 6.211156383539862E-4 82-83 0.004443365720532363 0.0 0.0 1.0059445544403078 6.450047013676011E-4 84-85 0.0044672547835459785 2.3889063013614855E-5 0.0 1.169751859524665 6.688937643812159E-4 86-87 0.004491143846559593 4.777812602722971E-5 0.0 1.4290198604114268 6.927828273948308E-4 88-89 0.004491143846559593 4.777812602722971E-5 0.0 1.7578528127938355 7.166718904084456E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7270 0.0 37.05414 1 GTATCAA 10755 0.0 33.17551 1 TATCAAC 13540 0.0 26.346706 2 TCAACGC 13525 0.0 26.3038 4 ATCAACG 13550 0.0 26.29095 3 CAACGCA 13735 0.0 25.867048 5 AACGCAG 14110 0.0 25.215654 6 GTACATG 9115 0.0 23.14272 1 TATACCG 105 0.0021973979 22.618025 5 TACATGG 9250 0.0 22.595192 2 GAGTACT 8850 0.0 22.464872 12-13 ACGCAGA 15995 0.0 22.125216 7 CGCAGAG 16205 0.0 21.635885 8 ACATGGG 9240 0.0 21.127796 3 GTACTTT 9390 0.0 20.993883 14-15 GCAGAGT 17165 0.0 20.038357 9 AGAGTAC 13865 0.0 19.770756 10-11 AGTACTT 9205 0.0 19.637339 12-13 CATGGGG 5295 0.0 18.747969 4 ACTTTTT 11040 0.0 18.199558 16-17 >>END_MODULE