FastQCFastQC Report
Thu 26 May 2016
SRR937091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612240
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141140.8754279759837246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97770.6064233612861608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66410.4119113779586166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22950.14234853371706446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.1344092690914504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143550.032.1146741
GGTATCA92900.030.4394111
GTACATG109600.026.6685561
ATCAACG173900.026.509023
TCAACGC174300.026.5027164
TATCAAC174200.026.3823382
CAACGCA175350.026.3160955
AACGCAG177850.025.9996176
TACATGG112300.025.6041642
ACATGGG110750.024.631433
GAGTACT91500.023.75471712-13
ACGCAGA197950.023.0707847
CGCAGAG198550.022.9299778
GTACTTT97250.022.08184814-15
AGTACTT96150.022.03765712-13
CATGGGA74650.021.9003014
GCAGAGT204900.021.5474179
CATGGGG37850.020.4664824
AGAGTAC163200.020.30914110-11
ATGGGAT29250.019.984225
Produced by FastQC (version 0.11.5)