FastQCFastQC Report
Thu 26 May 2016
SRR937091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612240
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152920.9484940207413288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113250.7024388428521808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.4833027340842555No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29410.18241700987446038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27600.17119039348980303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.13025356026398055No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.11232818935146133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16860.10457500124051011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA160350.031.3434751
GGTATCA106700.030.675051
TATCAAC199750.025.1825242
ATCAACG199650.025.1452143
TCAACGC200750.025.0074334
CAACGCA204200.024.5616665
GAGTACT111650.024.1627312-13
AACGCAG208350.024.1187766
GTACATG110700.024.1167741
TACATGG114450.022.8678782
GTACTTT117950.022.7114214-15
AGTACTT115650.022.60830912-13
ACATGGG113650.022.14913
ACGCAGA229250.021.7956337
CGCAGAG231000.021.527718
CATGGGA79050.020.6082884
TACTTTT127100.020.3663914-15
GCAGAGT239800.020.2425339
AGAGTAC192050.020.09426710-11
ATGGGAT30700.019.9572625
Produced by FastQC (version 0.11.5)