##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937091_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1612240 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14266424353694 33.0 31.0 34.0 31.0 34.0 2 32.11602304867762 33.0 31.0 34.0 30.0 34.0 3 32.28658574405796 33.0 31.0 34.0 30.0 34.0 4 36.040824567061975 37.0 35.0 37.0 35.0 37.0 5 35.97528655783258 37.0 35.0 37.0 35.0 37.0 6 36.03516225872079 37.0 35.0 37.0 35.0 37.0 7 35.97224668783804 37.0 35.0 37.0 35.0 37.0 8 35.9552436361832 37.0 35.0 37.0 35.0 37.0 9 37.62414404803255 39.0 37.0 39.0 35.0 39.0 10-11 37.557778928695484 39.0 37.0 39.0 35.0 39.0 12-13 37.45999975189798 39.0 37.0 39.0 35.0 39.0 14-15 38.60874218478638 40.0 38.0 41.0 34.0 41.0 16-17 38.54799688631965 40.0 38.0 41.0 34.0 41.0 18-19 38.65858588051407 40.0 38.0 41.0 34.5 41.0 20-21 38.74594694338312 40.0 38.0 41.0 34.0 41.0 22-23 38.67176164838982 40.0 38.0 41.0 34.0 41.0 24-25 38.56377369374287 40.0 38.0 41.0 34.0 41.0 26-27 38.38766405746043 40.0 38.0 41.0 34.0 41.0 28-29 38.20319586413933 40.0 38.0 41.0 34.0 41.0 30-31 37.999974569543 40.0 37.0 41.0 33.5 41.0 32-33 37.905537326948846 40.0 37.0 41.0 33.0 41.0 34-35 37.785168461271276 40.0 37.0 41.0 33.0 41.0 36-37 37.59108352354488 40.0 36.5 41.0 33.0 41.0 38-39 37.39889656626805 40.0 36.0 41.0 33.0 41.0 40-41 37.17680215104451 40.0 36.0 41.0 32.0 41.0 42-43 36.88232490199971 39.5 36.0 41.0 31.0 41.0 44-45 36.43504037860368 39.0 35.0 41.0 30.5 41.0 46-47 35.99273309184737 39.0 35.0 41.0 29.5 41.0 48-49 35.524553106237285 39.0 35.0 41.0 27.5 41.0 50-51 35.20258832431897 39.0 35.0 40.0 26.5 41.0 52-53 34.932083312658165 38.5 35.0 40.0 26.0 41.0 54-55 34.67177064208803 38.0 34.0 40.0 25.0 41.0 56-57 34.384946099836256 38.0 34.0 40.0 23.5 41.0 58-59 34.05198450602887 38.0 34.0 40.0 23.0 41.0 60-61 33.71393682082072 37.0 33.0 40.0 22.5 41.0 62-63 33.3387538455813 37.0 33.0 39.5 22.0 41.0 64-65 32.9235647298169 36.0 32.5 39.0 20.0 40.5 66-67 32.539804247506574 35.5 32.0 39.0 19.5 40.0 68-69 32.103477149804 35.0 31.5 38.5 18.0 40.0 70-71 31.62303286111249 35.0 31.0 37.5 14.0 40.0 72-73 31.30189922095966 35.0 31.0 37.0 12.0 39.0 74-75 30.87612700342381 35.0 31.0 36.5 8.0 39.0 76-77 29.50355468168511 33.5 28.5 35.0 7.0 37.5 78-79 30.201191199821366 34.0 30.0 36.0 6.5 37.5 80-81 30.04166253163301 34.0 30.0 35.0 2.0 37.0 82-83 29.724077370614797 34.0 30.0 35.0 2.0 37.0 84-85 29.356449101870687 34.0 30.0 35.0 2.0 36.0 86-87 29.01074343770158 34.0 29.0 35.0 2.0 36.0 88-89 28.666893576638714 34.0 29.0 35.0 2.0 35.5 90-91 28.257509738004266 34.0 29.0 35.0 2.0 35.0 92-93 27.826657011363075 33.5 27.5 35.0 2.0 35.0 94-95 27.381669912668087 33.0 27.0 35.0 2.0 35.0 96-97 26.780479953356817 33.0 25.5 35.0 2.0 35.0 98-99 26.101488922244826 33.0 24.5 35.0 2.0 35.0 100-101 23.222802126234306 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30712427028240796 1101 2 0.18487604440560546 1101 3 0.2797928839244008 1101 4 0.2428410955639606 1101 5 0.30507716023385 1101 6 0.23992524662157422 1101 7 0.2528643919653746 1101 8 0.23688001021680094 1101 9 0.3344718414862342 1101 10-11 0.32848754408856706 1101 12-13 0.2756879426258507 1101 14-15 0.49982208962432395 1101 16-17 0.38141614643351573 1101 18-19 0.3187548007604377 1101 20-21 0.3153183634847494 1101 22-23 0.3489554163075965 1101 24-25 0.3804033188408269 1101 26-27 0.37648989321269966 1101 28-29 0.39827668320946685 1101 30-31 0.40162458740619655 1101 32-33 0.41223500660859713 1101 34-35 0.369491804139912 1101 36-37 0.3500219786658434 1101 38-39 0.4027773154693506 1101 40-41 0.44466211176184345 1101 42-43 0.5096892834560336 1101 44-45 0.6487717844098242 1101 46-47 0.7007469094853178 1101 48-49 0.6344155125945434 1101 50-51 0.5223814513660727 1101 52-53 0.4456097146508853 1101 54-55 0.5375127433830471 1101 56-57 0.6256606254237411 1101 58-59 0.5201972326579707 1101 60-61 0.5534662591570054 1101 62-63 0.6800735791932837 1101 64-65 0.7646142711012018 1101 66-67 0.7182463414298041 1101 68-69 0.7173351144889963 1101 70-71 0.7446885552898159 1101 72-73 0.7766772355531231 1101 74-75 0.48561362043808387 1101 76-77 0.47605594268097917 1101 78-79 0.5550298042432118 1101 80-81 0.7259726748444777 1101 82-83 0.8319696150495304 1101 84-85 0.8568236747032287 1101 86-87 0.6725874361645836 1101 88-89 0.6753394067066623 1101 90-91 0.8429790179939154 1101 92-93 0.8805175715647771 1101 94-95 1.0252545605955774 1101 96-97 1.1371514968179568 1101 98-99 1.196241014933097 1101 100-101 1.2717234230378267 1102 1 0.24849398118410448 1102 2 0.08680074825927164 1102 3 0.1588982662719971 1102 4 0.15703405520459768 1102 5 0.1616720035072774 1102 6 0.13568599359925315 1102 7 0.18813639930900194 1102 8 0.15705495495074473 1102 9 0.25787671041558724 1102 10-11 0.37729720280304235 1102 12-13 0.3309797839944295 1102 14-15 0.47880359925263605 1102 16-17 0.36237701140805 1102 18-19 0.38752049844984526 1102 20-21 0.3545691074105761 1102 22-23 0.3844174297729168 1102 24-25 0.3614745060823026 1102 26-27 0.43584791954734925 1102 28-29 0.3298729804584255 1102 30-31 0.3914451098660692 1102 32-33 0.42992194439223397 1102 34-35 0.3574915595122832 1102 36-37 0.42977058779585064 1102 38-39 0.38310727497987784 1102 40-41 0.4267130086998563 1102 42-43 0.3046598471968167 1102 44-45 0.5644337090177416 1102 46-47 0.5687071946683773 1102 48-49 0.49151175258384683 1102 50-51 0.5865449417707325 1102 52-53 0.7143042424865769 1102 54-55 0.546090749320058 1102 56-57 0.5946534730632962 1102 58-59 0.6771420493401337 1102 60-61 0.6265376730387828 1102 62-63 0.7667657458926271 1102 64-65 0.5982506908798157 1102 66-67 0.7117866429333688 1102 68-69 0.5165524859290898 1102 70-71 0.5653703528952327 1102 72-73 0.5963264107442825 1102 74-75 0.5581842371815284 1102 76-77 0.523823685529365 1102 78-79 0.7035289717298028 1102 80-81 0.7153967266575734 1102 82-83 0.7722295079290049 1102 84-85 0.7587937965553735 1102 86-87 0.6691933776182353 1102 88-89 0.7278722920356557 1102 90-91 0.8258853621838007 1102 92-93 0.7417463997324489 1102 94-95 0.9507273724523451 1102 96-97 1.085553802553335 1102 98-99 1.0564276274459203 1102 100-101 1.1205544866703896 1103 1 0.11081208812135657 1103 2 -0.07035848269648426 1103 3 0.12542444312417445 1103 4 0.16393667976431914 1103 5 0.21486314714936583 1103 6 0.15000058079168355 1103 7 0.22952839838907124 1103 8 0.2415832016528583 1103 9 0.21919888955759603 1103 10-11 0.25185879708745773 1103 12-13 0.30063260916460166 1103 14-15 0.3573145947054641 1103 16-17 0.3709160798255908 1103 18-19 0.3848894392016362 1103 20-21 0.3198056902583204 1103 22-23 0.3955588095228748 1103 24-25 0.3172955215586839 1103 26-27 0.4164137035280078 1103 28-29 0.2912420312022519 1103 30-31 0.28987528617828673 1103 32-33 0.39098570476960504 1103 34-35 0.32504797417222164 1103 36-37 0.2911309516694658 1103 38-39 0.4331989558330207 1103 40-41 0.34583887207476494 1103 42-43 0.3177229626209481 1103 44-45 0.3670714552811347 1103 46-47 0.4232558648897822 1103 48-49 0.36653831315820184 1103 50-51 0.25445964295489887 1103 52-53 0.3248927873170757 1103 54-55 0.3550661611027053 1103 56-57 0.4567400342876127 1103 58-59 0.5504190492576413 1103 60-61 0.4494776973870245 1103 62-63 0.5147437049905932 1103 64-65 0.4778231718749524 1103 66-67 0.38387197924648575 1103 68-69 0.3489143271955939 1103 70-71 0.39896991692683414 1103 72-73 0.38964702000011187 1103 74-75 0.3937536583984915 1103 76-77 0.3038396343730767 1103 78-79 0.3917818858935256 1103 80-81 0.473164600986685 1103 82-83 0.558800294047014 1103 84-85 0.5216416150885301 1103 86-87 0.34051501929921457 1103 88-89 0.5325433283855219 1103 90-91 0.857963661507096 1103 92-93 0.7282842507382519 1103 94-95 0.6753232767492818 1103 96-97 1.112136250325868 1103 98-99 0.9387777195642428 1103 100-101 1.163924154869445 1104 1 0.18785034571324388 1104 2 0.07092468524082562 1104 3 0.14504703139454023 1104 4 0.07163345134743082 1104 5 0.030047726986666135 1104 6 0.07988340161028873 1104 7 0.17239223415720062 1104 8 0.07641965124611261 1104 9 0.1000472008144655 1104 10-11 0.1783090292097782 1104 12-13 0.23684355831065318 1104 14-15 0.2177111192593273 1104 16-17 0.32020919814409154 1104 18-19 0.30155965513928606 1104 20-21 0.1767270348641361 1104 22-23 0.1432735226708033 1104 24-25 0.1945355552057677 1104 26-27 0.16200085055300661 1104 28-29 0.2728366210484978 1104 30-31 0.3237080463043611 1104 32-33 0.4175320334037025 1104 34-35 0.2121339073465407 1104 36-37 0.18416435385865526 1104 38-39 0.08473862958403089 1104 40-41 0.16835447112072188 1104 42-43 0.22846434321638043 1104 44-45 0.3057480386109219 1104 46-47 0.45443532312906143 1104 48-49 0.45533974209470074 1104 50-51 0.3008022523079319 1104 52-53 0.37280728005526953 1104 54-55 0.2958217299088659 1104 56-57 0.39235936432721985 1104 58-59 0.4571981124630611 1104 60-61 0.6647325527897152 1104 62-63 0.613790991927857 1104 64-65 0.5136098056753653 1104 66-67 0.6072304857842354 1104 68-69 0.391638676614221 1104 70-71 0.612977647239024 1104 72-73 0.5596493358994259 1104 74-75 0.6234622543778734 1104 76-77 0.5003030964029733 1104 78-79 0.642403451973852 1104 80-81 0.6972591375938642 1104 82-83 0.7446625273192993 1104 84-85 0.7523477780426617 1104 86-87 0.49322595247354073 1104 88-89 0.7500781467338342 1104 90-91 0.8551879097715052 1104 92-93 0.8175468630726748 1104 94-95 0.7981058697046883 1104 96-97 0.93123656337157 1104 98-99 1.0210447187976008 1104 100-101 1.1752960190885382 1105 1 0.1465185671390543 1105 2 -0.05574499724866655 1105 3 0.10813431164939402 1105 4 0.13142213879106635 1105 5 0.11350440161744757 1105 6 0.06850562441810126 1105 7 0.17489273191436894 1105 8 0.12707522671195193 1105 9 0.16289123885482581 1105 10-11 0.2305398597029651 1105 12-13 0.29740248836698413 1105 14-15 0.2703870032174862 1105 16-17 0.30608369160297144 1105 18-19 0.18876495361790546 1105 20-21 0.15818667847000256 1105 22-23 0.2314852422168414 1105 24-25 0.2377368511311886 1105 26-27 0.29600421626983575 1105 28-29 0.2930208570790924 1105 30-31 0.2844703248024203 1105 32-33 0.20630255856679014 1105 34-35 0.1787195786745599 1105 36-37 0.17932256743747388 1105 38-39 0.20658519154601152 1105 40-41 0.08053144136069079 1105 42-43 0.09168158933093906 1105 44-45 0.34109684926409756 1105 46-47 0.2571587868162126 1105 48-49 0.14655205894933943 1105 50-51 0.1686321701487259 1105 52-53 0.16758072002575375 1105 54-55 0.08290317469295871 1105 56-57 0.18944097015039318 1105 58-59 0.14135197652265674 1105 60-61 0.18529175368450979 1105 62-63 0.16937106934373247 1105 64-65 0.12708691030977803 1105 66-67 0.3620106015566975 1105 68-69 0.18803550033791083 1105 70-71 0.028956969820807643 1105 72-73 0.18025315194009295 1105 74-75 0.2068708002827968 1105 76-77 0.055530534827482825 1105 78-79 -0.02642662837000742 1105 80-81 0.19050556106223837 1105 82-83 0.3529278216401863 1105 84-85 0.546337799475495 1105 86-87 0.32842001920813857 1105 88-89 0.3047523793094058 1105 90-91 0.45673824034082244 1105 92-93 0.6046702146180678 1105 94-95 0.7796310269373912 1105 96-97 1.033901540591863 1105 98-99 1.0499419011992508 1105 100-101 1.0376676270198573 1106 1 0.35343864891947163 1106 2 0.22388846674233776 1106 3 0.23889269045246664 1106 4 0.21431553341630405 1106 5 0.09877133828103268 1106 6 0.2534031601536313 1106 7 0.22388979320193414 1106 8 0.3031019312302732 1106 9 0.34936274376208587 1106 10-11 0.3086197562186115 1106 12-13 0.3205011153762243 1106 14-15 0.5103189830936046 1106 16-17 0.3842063448652979 1106 18-19 0.4086846056604614 1106 20-21 0.3661509364342663 1106 22-23 0.4324879638899617 1106 24-25 0.5094954773590885 1106 26-27 0.40072916233556555 1106 28-29 0.45857063938864684 1106 30-31 0.5089043947672138 1106 32-33 0.4331896069622232 1106 34-35 0.3682226888480642 1106 36-37 0.20538334712954054 1106 38-39 0.20718552231394938 1106 40-41 0.2888032440459085 1106 42-43 0.21546996730926793 1106 44-45 0.32212717833911597 1106 46-47 0.025326931520666562 1106 48-49 -0.21861378079749727 1106 50-51 -0.17040973146301042 1106 52-53 -0.12952440948161126 1106 54-55 -0.012348873073051436 1106 56-57 0.08568879433117615 1106 58-59 0.058837098477042105 1106 60-61 0.018760953634156863 1106 62-63 0.1316520224068114 1106 64-65 0.15046204541675223 1106 66-67 0.2633629735794685 1106 68-69 0.2802585473095647 1106 70-71 0.2131706146489769 1106 72-73 0.22056147800260817 1106 74-75 0.16989771749965854 1106 76-77 0.19870616738859326 1106 78-79 0.26100831409286585 1106 80-81 0.4474929250328401 1106 82-83 0.5126468425323907 1106 84-85 0.4434943673024563 1106 86-87 0.27354987383519713 1106 88-89 0.4285053798780467 1106 90-91 0.640526922174196 1106 92-93 0.6971669481762532 1106 94-95 0.5860412833476509 1106 96-97 0.7274324665250234 1106 98-99 0.717352361626471 1106 100-101 0.8548612651632155 1107 1 0.2310225536878434 1107 2 0.11269286622635377 1107 3 0.10823435202713938 1107 4 0.1523968695558935 1107 5 0.201582119732592 1107 6 0.15846120890099513 1107 7 0.19140059954769129 1107 8 0.12019293084554761 1107 9 0.32400686986252225 1107 10-11 0.18680112177862185 1107 12-13 0.2366846082587415 1107 14-15 0.289841240771203 1107 16-17 0.3910511182149179 1107 18-19 0.2716182739339317 1107 20-21 0.28159552420895295 1107 22-23 0.19156189966292203 1107 24-25 0.25259525783750547 1107 26-27 0.22662139308216211 1107 28-29 0.30507753055572806 1107 30-31 0.3826647767800253 1107 32-33 0.45299067967592066 1107 34-35 0.4550853407592541 1107 36-37 0.39074103151634887 1107 38-39 0.42103801286546627 1107 40-41 0.5170015829238537 1107 42-43 0.5356342690269642 1107 44-45 0.4455118842480985 1107 46-47 0.4645081386630423 1107 48-49 0.5548523970376067 1107 50-51 0.46600821951804505 1107 52-53 0.6485573792797865 1107 54-55 0.7036513079756759 1107 56-57 0.7176619991199189 1107 58-59 0.7602017532397625 1107 60-61 0.677036011909216 1107 62-63 0.5258143325885385 1107 64-65 0.6666161178815102 1107 66-67 0.5194982622390185 1107 68-69 0.5692688897179679 1107 70-71 0.5639355455109971 1107 72-73 0.6801344293231324 1107 74-75 0.666896834887126 1107 76-77 0.584629629040208 1107 78-79 0.6533284535003823 1107 80-81 0.74286826167031 1107 82-83 0.6677976787511852 1107 84-85 0.8012364633940656 1107 86-87 0.7118145218085843 1107 88-89 0.923892032060813 1107 90-91 1.1202687014305361 1107 92-93 1.1319113300080126 1107 94-95 1.1233203473257412 1107 96-97 1.1954275782094541 1107 98-99 0.9824767194692718 1107 100-101 1.0774957504190574 1108 1 0.08979082133721761 1108 2 -0.06506028941953446 1108 3 -0.013852924588228177 1108 4 0.08220502097792348 1108 5 0.11093458487050611 1108 6 0.08002244251061796 1108 7 0.030882800194937943 1108 8 0.10818045925962139 1108 9 0.10380502279452486 1108 10-11 0.1604195957233756 1108 12-13 0.23305413612596482 1108 14-15 0.24322623977135294 1108 16-17 0.2617885986770503 1108 18-19 0.15764711855739932 1108 20-21 0.19237471265803663 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-0.774509254758037 2316 80-81 -0.8866325174259373 2316 82-83 -0.6273449557927435 2316 84-85 -0.6650526412445501 2316 86-87 -0.6949815946962694 2316 88-89 -0.7487047556654218 2316 90-91 -0.5376630976824899 2316 92-93 -1.1431486004726281 2316 94-95 -0.9012331705934677 2316 96-97 -0.6339203781611573 2316 98-99 -0.7111916252508514 2316 100-101 -0.8134996948644115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 14.0 5 43.0 6 102.0 7 211.0 8 568.0 9 981.0 10 1856.0 11 3135.0 12 4863.0 13 6865.0 14 8643.0 15 12391.0 16 17122.0 17 24629.0 18 21115.0 19 11537.0 20 9135.0 21 9016.0 22 9375.0 23 9934.0 24 11357.0 25 13364.0 26 16004.0 27 19761.0 28 24528.0 29 30572.0 30 38150.0 31 47924.0 32 59810.0 33 79283.0 34 108486.0 35 157283.0 36 237615.0 37 347876.0 38 250113.0 39 18575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.240769977574374 19.639515010902638 15.34477464260125 20.77494036892174 2 15.588929089579448 21.831869588491177 39.56390969048914 23.015291631440235 3 19.238527421051586 27.07127935134277 30.250367201921357 23.439826025684283 4 12.967796357862351 17.62274847417258 37.19092690914504 32.21852825882003 5 13.167978940760966 38.61546916228243 32.955860698393636 15.26069119856297 6 30.16242018858258 37.144826012040404 17.429436596829998 15.26331720254702 7 27.48455564928299 34.577110107676276 20.67520964620652 17.263124596834217 8 27.274909442762862 33.24579467076862 19.66419391653848 19.815101969930033 9 27.755793182156502 16.465228501960006 20.446955788220116 35.332022527663376 10-11 24.809969005797537 27.479241575525187 26.402445794259776 21.308343624417503 12-13 26.802162222511676 25.123617597985398 27.46503290473444 20.60918727476849 14-15 23.60988438445889 25.44069121222647 27.230251079243782 23.71917332407086 16-17 21.302194462362927 29.02747109611472 27.40829529102367 22.262039150498687 18-19 21.180190294248995 26.48997667841016 31.59631940653997 20.733513620800874 20-21 22.54623416876499 25.301846761615572 31.670905696903084 20.481013372716358 22-23 22.09695826924031 25.430488016672452 31.76766486379199 20.70488885029524 24-25 21.830194846882094 25.621437685035687 31.923459346267713 20.624908121814503 26-27 21.522736816380505 25.789088139316924 31.94871136434544 20.739463679957126 28-29 21.1806092913063 25.85253114640415 32.08863501664977 20.87822454563978 30-31 21.385773587211506 25.69289764981648 31.93506373060964 20.986265032362365 32-33 21.259269390163986 25.974109749331436 31.68682354889521 21.079797311609365 34-35 21.717121594245867 25.633793119703657 31.72938435601582 20.919700930034647 36-37 21.947061401318766 25.52049589734699 31.415650217080692 21.116792484253548 38-39 21.561692004095008 25.932937250904274 31.532375040046052 20.97299570495467 40-41 21.64441613203562 25.8917943568021 31.518430737186065 20.94535877397621 42-43 21.58168292343291 26.134807674355642 30.861142891346333 21.422366510865118 44-45 21.581598159071596 26.299318335473227 30.521234563306727 21.597848942148453 46-47 22.16586508939747 26.496429187253987 29.949672442902354 21.388033280446187 48-49 22.2437037753778 26.907413058634095 29.187414249526743 21.661468916461363 50-51 22.138225827010086 27.15485332970277 29.027746205043485 21.67917463824366 52-53 22.361368003582644 27.37117436971923 28.6651991231955 21.60225850350263 54-55 22.018781963608394 27.259743763898065 28.685944851242258 22.035529421251283 56-57 22.189648322837012 27.520799587404166 28.369530058183333 21.92002203157549 58-59 22.397040101599195 26.986836453518215 28.61760277381929 21.9985206710633 60-61 22.233063033251348 27.247575626286853 28.98328733569633 21.536074004765478 62-63 22.16270530442118 27.180010420284823 29.21205899866025 21.445225276633753 64-65 22.136330494936693 27.28703264068268 28.910057550489647 21.66657931389098 66-67 22.169124944177046 27.498883540912022 28.574157693643627 21.757833821267305 68-69 22.072210092790158 27.49094427628641 28.69734034635042 21.739505284573017 70-71 22.31703527459052 27.46268073490436 28.448298854324765 21.771985136180348 72-73 22.21789766216351 27.580906919168598 28.18277196581027 22.018423452857625 74-75 22.0499387960863 28.08023775712389 27.913574553561876 21.956248893227933 76-77 22.1399585356285 27.701053356303625 28.255658708215808 21.90332939985207 78-79 22.012544677815768 27.784616100295395 28.031602043743554 22.17123717814528 80-81 22.12545385693493 28.17053204267953 27.71310069847524 21.9909134019103 82-83 22.168124906185145 27.866454784650713 27.92556589741586 22.039854411748287 84-85 21.999768017705087 27.9869271153808 27.676976439530776 22.33632842738334 86-87 22.00284886943054 27.891380727860398 27.715163918538337 22.390606484170725 88-89 22.134259009432295 27.822043670029096 27.491569115267268 22.552128205271345 90-91 22.008599226665044 27.76153396706938 27.779955564873177 22.4499112413924 92-93 21.97040204014354 27.89768517642695 27.68676738164522 22.445145401784288 94-95 21.804703650890037 27.561456535236445 27.729483940636516 22.904355873237 96-97 21.89125192863844 27.694333098149283 27.676686541011186 22.73772843220109 98-99 21.66938809055286 27.987123245611993 27.448637969988354 22.89485069384679 100-101 21.302689573298036 28.294606339309748 27.524273892121826 22.87843019527039 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1635.0 1 1164.0 2 771.0 3 850.5 4 747.0 5 731.0 6 1224.5 7 2572.5 8 4309.5 9 5086.0 10 4475.5 11 3679.0 12 3674.5 13 3949.0 14 4089.0 15 4207.5 16 4369.0 17 4561.5 18 4699.0 19 4817.0 20 5109.5 21 5618.0 22 6269.0 23 7150.5 24 8007.0 25 9037.5 26 10479.5 27 12146.5 28 13503.0 29 15461.5 30 18438.5 31 21015.5 32 24598.0 33 28079.5 34 31029.5 35 34247.0 36 38758.5 37 43220.5 38 45136.0 39 45945.0 40 46276.0 41 47319.0 42 48027.5 43 49816.0 44 53113.5 45 55278.5 46 55112.0 47 56555.0 48 64047.0 49 63423.0 50 55474.5 51 52081.0 52 50456.5 53 50351.5 54 49748.5 55 47196.0 56 44050.0 57 40258.0 58 37800.0 59 35268.5 60 30134.5 61 24722.5 62 20292.0 63 15313.0 64 10411.5 65 7481.5 66 5460.5 67 4164.0 68 2967.0 69 1841.5 70 1202.0 71 750.5 72 544.0 73 435.5 74 284.0 75 202.5 76 145.5 77 81.0 78 40.5 79 23.5 80 14.5 81 10.0 82 7.5 83 5.0 84 4.0 85 2.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015196248697464396 2 0.003163300749268099 3 0.0029772242346052698 4 0.0 5 0.0016126631270778544 6 1.2405100977521956E-4 7 0.0 8 0.0 9 0.0 10-11 1.8607651466282936E-4 12-13 6.202550488760978E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024500074430605866 22-23 0.0 24-25 0.003163300749268099 26-27 0.0019227906515159035 28-29 0.003442415521262343 30-31 0.01609561851833474 32-33 7.132933062075125E-4 34-35 0.002605071205279611 36-37 0.003194313501711904 38-39 0.002077854413734928 40-41 0.0042797598372450755 42-43 0.0024500074430605866 44-45 6.202550488760978E-4 46-47 1.2405100977521956E-4 48-49 2.481020195504391E-4 50-51 9.303825733141468E-5 52-53 0.0013645611075274154 54-55 0.0034114027688185384 56-57 5.272167915446832E-4 58-59 0.00139557385997122 60-61 0.0012094973453083907 62-63 0.0 64-65 2.1708926710663426E-4 66-67 0.0 68-69 0.0 70-71 1.8607651466282936E-4 72-73 9.303825733141468E-5 74-75 4.031657817694636E-4 76-77 7.753188110951224E-4 78-79 1.5506376221902446E-4 80-81 0.005303180667890636 82-83 0.0013645611075274154 84-85 0.0029772242346052698 86-87 0.0013335483550836102 88-89 8.063315635389272E-4 90-91 1.2405100977521956E-4 92-93 3.101275244380489E-5 94-95 1.8607651466282936E-4 96-97 0.0017057013844092692 98-99 0.002015828908847318 100-101 8.68357068426537E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1612240.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.12261929686826 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6821236218807 33.06167298203051 2 14.489981621998908 13.366318119401463 3 5.4477402722753965 7.537921518191264 4 2.6131731598338694 4.821055632312472 5 1.4612886990709517 3.3699231175032693 6 0.9171577250288896 2.5381029952013545 7 0.6262404973914467 2.021869643462698 8 0.43794637078756515 1.6159386985823976 9 0.3332516576352985 1.3833395401646835 >10 1.7660604311851986 14.919330571609349 >50 0.14350082987221421 4.574570153653198 >100 0.07599137730177144 6.383267988809369 >500 0.0037859658696942754 1.2064278663047399 >1k 0.0013521306677479555 1.0543849023320877 >5k 1.3521306677479557E-4 0.4859387921554166 >10k+ 2.7042613354959113E-4 1.6599374782855139 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15292 0.9484940207413288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11325 0.7024388428521808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7792 0.4833027340842555 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2941 0.18241700987446038 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2760 0.17119039348980303 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.13025356026398055 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1811 0.11232818935146133 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1686 0.10457500124051011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.202550488760979E-5 0.0 5 0.0 0.0 0.0 6.202550488760979E-5 0.0 6 0.0 0.0 0.0 6.202550488760979E-5 0.0 7 0.0 0.0 0.0 6.202550488760979E-5 0.0 8 0.0 0.0 0.0 6.202550488760979E-5 0.0 9 0.0 0.0 0.0 6.202550488760979E-5 0.0 10-11 0.0 0.0 0.0 6.202550488760979E-5 0.0 12-13 0.0 0.0 0.0 6.202550488760979E-5 0.0 14-15 0.0 0.0 0.0 6.202550488760979E-5 0.0 16-17 0.0 0.0 0.0 1.2405100977521958E-4 0.0 18-19 0.0 0.0 0.0 1.8607651466282936E-4 0.0 20-21 0.0 0.0 0.0 2.4810201955043917E-4 0.0 22-23 0.0 0.0 0.0 4.651912866570734E-4 0.0 24-25 0.0 0.0 0.0 8.063315635389272E-4 0.0 26-27 0.0 0.0 0.0 0.0015506376221902447 0.0 28-29 0.0 0.0 0.0 0.0033493772639309285 0.0 30-31 0.0 0.0 0.0 0.008342430407383515 0.0 32-33 0.0 0.0 0.0 0.015165235945020592 0.0 34-35 0.0 0.0 0.0 0.02087158239468069 0.0 36-37 0.0 0.0 0.0 0.024655138192824888 0.0 38-39 0.0 0.0 0.0 0.027818438942092988 0.0 40-41 0.0 0.0 0.0 0.032439339056219915 0.0 42-43 0.0 0.0 0.0 0.039262144593857 0.0 44-45 0.0 0.0 0.0 0.04651912866570734 0.0 46-47 0.0 0.0 0.0 0.054675482558428024 0.0 48-49 0.0 0.0 0.0 0.06388627003423808 0.0 50-51 0.0 0.0 0.0 0.07675656229841711 0.0 52-53 0.0 0.0 0.0 0.10314841462809507 0.0 54-55 0.0 0.0 0.0 0.13354091202302387 0.0 56-57 0.0 0.0 0.0 0.1730511586364313 0.0 58-59 0.0 0.0 0.0 0.2294943680841562 0.0 60-61 0.0 0.0 0.0 0.31155411105046393 0.0 62-63 0.0 0.0 0.0 0.3658264278271225 0.0 64-65 0.0 0.0 0.0 0.4168113928447378 0.0 66-67 0.0 0.0 0.0 0.5202079094923833 0.0 68-69 0.0 0.0 0.0 0.6501823549843695 0.0 70-71 0.0 0.0 0.0 0.7517491192378306 0.0 72-73 0.0 0.0 0.0 0.8415310375626457 0.0 74-75 0.0 0.0 0.0 1.0305847764600804 0.0 76-77 0.0 0.0 0.0 1.1616446682875998 0.0 78-79 0.0 0.0 0.0 1.25855951967449 0.0 80-81 0.0 0.0 0.0 1.3651193370714036 0.0 82-83 0.0 0.0 0.0 1.5522192725648787 0.0 84-85 0.0 3.1012752443804896E-5 0.0 1.7486230337914952 0.0 86-87 0.0 6.202550488760979E-5 0.0 2.059680940802858 0.0 88-89 0.0 6.202550488760979E-5 0.0 2.4737632114325407 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16035 0.0 31.343475 1 GGTATCA 10670 0.0 30.67505 1 TATCAAC 19975 0.0 25.182524 2 ATCAACG 19965 0.0 25.145214 3 TCAACGC 20075 0.0 25.007433 4 CAACGCA 20420 0.0 24.561666 5 GAGTACT 11165 0.0 24.16273 12-13 AACGCAG 20835 0.0 24.118776 6 GTACATG 11070 0.0 24.116774 1 TACATGG 11445 0.0 22.867878 2 GTACTTT 11795 0.0 22.71142 14-15 AGTACTT 11565 0.0 22.608309 12-13 ACATGGG 11365 0.0 22.1491 3 ACGCAGA 22925 0.0 21.795633 7 CGCAGAG 23100 0.0 21.52771 8 CATGGGA 7905 0.0 20.608288 4 TACTTTT 12710 0.0 20.36639 14-15 GCAGAGT 23980 0.0 20.242533 9 AGAGTAC 19205 0.0 20.094267 10-11 ATGGGAT 3070 0.0 19.957262 5 >>END_MODULE