FastQCFastQC Report
Thu 26 May 2016
SRR937090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495456
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141110.9435917873879272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94970.6350571330751289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65170.43578681017696275No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.14778101127682794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21540.14403633406800334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.10465035413947318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135150.034.5331761
GGTATCA85800.033.623471
TATCAAC165200.028.1077212
ATCAACG165300.028.0313363
TCAACGC165550.027.990884
CAACGCA168000.027.5845245
AACGCAG172200.026.964236
GTACATG99400.026.3933051
TACATGG101050.025.6801432
ACGCAGA188350.024.4747437
ACATGGG99100.024.4573023
CGCAGAG188150.024.2995058
GAGTACT91700.024.27654512-13
GCAGAGT195200.022.9836379
GTACTTT96400.022.82183814-15
AGTACTT94750.022.69269212-13
CATGGGA68850.022.1585944
CCATGCG1302.931428E-421.9348419
TAGCACG1100.002868266321.6032164
AGAGTAC158600.020.70521210-11
Produced by FastQC (version 0.11.5)