FastQCFastQC Report
Thu 26 May 2016
SRR937090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495456
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139840.9350993944321999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104660.6998534226349689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71120.4755740055207241No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27840.18616395266728009No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.17065028994500672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.12337374018359618No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.11153788543427556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16060.10739199281021976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148000.031.6605151
GGTATCA97800.031.2931861
GTACCGT700.00839866427.1386816
ACTACGC908.9568534E-426.3848298
TATCAAC180550.025.8133012
ATCAACG182100.025.6701353
TCAACGC183050.025.5836814
CAACGCA184750.025.3225635
TATCACG950.001225436625.0045152
AACGCAG188150.024.9390286
GTACATG105450.024.4712431
TACATGG107950.023.6332632
GAGTACT104650.023.37389812-13
ACATGGG108450.022.7342593
AGTACTT108550.022.57787312-13
GTACTTT109750.022.24631114-15
ACGCAGA210250.022.1820817
CGCAGAG211050.022.030498
CATGGGG34100.021.5890314
GCAGAGT219250.020.9249469
Produced by FastQC (version 0.11.5)