FastQCFastQC Report
Thu 26 May 2016
SRR937089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548455
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138900.8970231617967587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94770.6120294099602507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.4237126684340197No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21930.14162503915192887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21710.1402042681253249No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.10352254343845962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135950.035.0621341
GGTATCA84350.033.410751
ATCAACG165850.028.5949653
TATCAAC166550.028.5033172
TCAACGC167150.028.4014
CAACGCA168700.028.1682245
AACGCAG172750.027.560186
GTACATG104900.024.9174631
ACGCAGA190650.024.8455547
GAGTACT92500.024.77974512-13
CGCAGAG190250.024.6972128
TACATGG106350.024.1732062
GTACTTT97050.023.47038814-15
GCAGAGT194950.023.443059
GTTCGGG1252.2485618E-422.8117121
ACATGGG106750.022.7916933
AGTACTT95650.022.5233812-13
CATGGGG35150.022.306594
AGAGTAC159750.021.15871610-11
ACGTAAC1353.7836214E-421.1219561
Produced by FastQC (version 0.11.5)