##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548455 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876324465354177 33.0 31.0 34.0 30.0 34.0 2 31.975017678912206 33.0 31.0 34.0 30.0 34.0 3 31.9723149849366 33.0 31.0 34.0 30.0 34.0 4 35.55773722839863 37.0 35.0 37.0 33.0 37.0 5 35.556693607499085 37.0 35.0 37.0 33.0 37.0 6 35.66704489313542 37.0 35.0 37.0 33.0 37.0 7 35.61450800959666 37.0 35.0 37.0 33.0 37.0 8 35.5848436021712 37.0 35.0 37.0 33.0 37.0 9 37.1950279472119 39.0 37.0 39.0 34.0 39.0 10-11 37.07436896777756 39.0 37.0 39.0 33.0 39.0 12-13 36.97528891701728 39.0 37.0 39.0 33.0 39.0 14-15 38.116073440945975 40.0 38.0 41.0 33.0 41.0 16-17 38.057880920013815 40.0 38.0 41.0 33.0 41.0 18-19 38.06054648020123 40.0 38.0 41.0 33.0 41.0 20-21 37.97689245086231 40.0 38.0 41.0 33.0 41.0 22-23 37.85197406447071 40.0 38.0 41.0 33.0 41.0 24-25 37.952960854529195 40.0 38.0 41.0 33.0 41.0 26-27 37.89178503734367 40.0 38.0 41.0 33.0 41.0 28-29 37.789481773768046 40.0 37.5 41.0 33.0 41.0 30-31 37.56463991527038 40.0 37.0 41.0 32.5 41.0 32-33 37.308944399417484 40.0 36.0 41.0 32.0 41.0 34-35 37.09410250862957 39.5 36.0 41.0 31.0 41.0 36-37 36.886737102466654 39.0 35.5 41.0 31.0 41.0 38-39 36.70654329638252 39.0 35.0 41.0 30.5 41.0 40-41 36.49083376656086 39.0 35.0 41.0 30.0 41.0 42-43 36.20756399120413 39.0 35.0 41.0 30.0 41.0 44-45 35.8428569122125 39.0 35.0 40.0 28.5 41.0 46-47 35.36335670071135 38.5 35.0 40.0 26.5 41.0 48-49 34.9847318779041 38.5 35.0 40.0 24.5 41.0 50-51 34.222821134614826 38.0 34.0 39.5 23.5 40.5 52-53 34.38656531833344 38.0 34.0 39.5 24.0 40.5 54-55 34.769268076889546 39.0 35.0 40.0 24.0 41.0 56-57 34.65464479109822 38.5 34.5 40.0 23.5 41.0 58-59 34.39653880803769 38.0 34.0 40.0 23.0 41.0 60-61 34.10954209195617 38.0 34.0 40.0 22.5 41.0 62-63 33.76163304713408 37.0 34.0 40.0 22.0 41.0 64-65 33.41315181907127 37.0 33.5 40.0 20.5 41.0 66-67 33.03430031870477 36.0 33.0 39.0 19.5 41.0 68-69 32.63140743515311 36.0 33.0 39.0 18.0 41.0 70-71 32.23337488012244 35.0 32.0 38.5 15.5 40.0 72-73 31.833590256093977 35.0 32.0 37.5 12.0 40.0 74-75 31.397931486546266 35.0 31.5 37.0 8.0 39.0 76-77 30.982094410234716 35.0 31.0 36.5 7.0 39.0 78-79 30.560759272952716 35.0 31.0 36.0 3.5 39.0 80-81 30.127769615519988 34.5 30.5 36.0 2.0 37.5 82-83 29.72917553303131 34.0 30.0 35.0 2.0 37.0 84-85 29.354768462758038 34.0 29.5 35.0 2.0 36.5 86-87 29.018076082288474 34.0 29.0 35.0 2.0 36.0 88-89 28.672085724157306 34.0 29.0 35.0 2.0 36.0 90-91 28.291903865465898 34.0 29.0 35.0 2.0 35.5 92-93 27.935514109225004 34.0 28.5 35.0 2.0 35.0 94-95 27.547130849782526 34.0 27.0 35.0 2.0 35.0 96-97 27.061877807233664 34.0 26.5 35.0 2.0 35.0 98-99 26.437543874378008 33.5 25.0 35.0 2.0 35.0 100-101 23.934883157728187 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2963992511542166 1101 2 0.2672049145083939 1101 3 0.22121901495586727 1101 4 0.24088271593609534 1101 5 0.25530061947484484 1101 6 0.22275786375528384 1101 7 0.08261000560156617 1101 8 0.07173713989868702 1101 9 0.2090428469964607 1101 10-11 0.23297087068713296 1101 12-13 0.38411436971713897 1101 14-15 0.35215252751571313 1101 16-17 0.43741451798111797 1101 18-19 0.41314456504903774 1101 20-21 0.41272834612137643 1101 22-23 0.39712032258834995 1101 24-25 0.4479768252831917 1101 26-27 0.44845337282089304 1101 28-29 0.3291853015124744 1101 30-31 0.45592283162389435 1101 32-33 0.5375240088868978 1101 34-35 0.5586535847309761 1101 36-37 0.5087203260340871 1101 38-39 0.656991276528224 1101 40-41 0.622475035657537 1101 42-43 0.6285899743003682 1101 44-45 0.7145180607141768 1101 46-47 0.7888152881117989 1101 48-49 0.8335185881014624 1101 50-51 0.8685212170479488 1101 52-53 0.926299948210584 1101 54-55 1.0494042730283937 1101 56-57 1.0154575136241775 1101 58-59 0.9094529739237558 1101 60-61 1.0524310283933787 1101 62-63 1.1278168159742563 1101 64-65 1.1403771183217444 1101 66-67 1.0218542898472762 1101 68-69 0.970990451474286 1101 70-71 0.9223659141457929 1101 72-73 0.7983737493151892 1101 74-75 0.9074939633919676 1101 76-77 0.9275211083735506 1101 78-79 0.7538681028046099 1101 80-81 0.6991069818461746 1101 82-83 0.6688965124384012 1101 84-85 0.5720472250249209 1101 86-87 0.813258075863569 1101 88-89 0.7948841661798447 1101 90-91 0.8946283538630162 1101 92-93 1.012470928037228 1101 94-95 0.8296948836363214 1101 96-97 0.5425504334127389 1101 98-99 0.26057349355344783 1101 100-101 0.7158856937017539 1102 1 0.27714187310009564 1102 2 0.3976337319763523 1102 3 0.3061525078672851 1102 4 0.15448090997916353 1102 5 0.35152426233129574 1102 6 0.2860189004290561 1102 7 0.335645394962377 1102 8 0.2692033511839682 1102 9 0.49562953001014876 1102 10-11 0.49541796179259734 1102 12-13 0.34141409434762693 1102 14-15 0.340359803887452 1102 16-17 0.6198594684048757 1102 18-19 0.6041040769735986 1102 20-21 0.6257261762485555 1102 22-23 0.5398984409291288 1102 24-25 0.28839727463174825 1102 26-27 0.4981045390059009 1102 28-29 0.2727184966745355 1102 30-31 0.26885595478120194 1102 32-33 0.458611669802913 1102 34-35 0.263118852170237 1102 36-37 0.26071602520992343 1102 38-39 0.4989471784849968 1102 40-41 0.5508789628354336 1102 42-43 0.3246301996557648 1102 44-45 0.048324776616517795 1102 46-47 0.23098136609099384 1102 48-49 0.17215156822231847 1102 50-51 0.26604143189454277 1102 52-53 0.35614834902404624 1102 54-55 0.46700554186882925 1102 56-57 0.6705946632091795 1102 58-59 0.42633370876140475 1102 60-61 0.5151739227896499 1102 62-63 0.49485956149585775 1102 64-65 0.606835068521697 1102 66-67 0.5479890944127632 1102 68-69 0.6767464526808524 1102 70-71 0.42909154686319795 1102 72-73 0.3890374579489517 1102 74-75 0.45379447618254076 1102 76-77 0.3747421142993481 1102 78-79 0.49830962813763335 1102 80-81 0.4537624780464604 1102 82-83 0.5500901370990547 1102 84-85 0.5028136863807511 1102 86-87 0.5374648073345085 1102 88-89 0.37033492563533343 1102 90-91 0.34007551308107864 1102 92-93 0.2567339010549041 1102 94-95 0.2516757926476174 1102 96-97 0.2841088496974855 1102 98-99 0.08532755841610395 1102 100-101 0.1871616373045235 1103 1 0.2936743808400344 1103 2 0.41607542444281265 1103 3 0.33990551784321 1103 4 0.3995927090847715 1103 5 0.41014483336672214 1103 6 0.32610489974106116 1103 7 0.3375064200741775 1103 8 0.3269536091819063 1103 9 0.4358772080287281 1103 10-11 0.4155882404303739 1103 12-13 0.6138151951388267 1103 14-15 0.5603305641213652 1103 16-17 0.6167600532002027 1103 18-19 0.6096923122677254 1103 20-21 0.592126245048 1103 22-23 0.7079995761200522 1103 24-25 0.4347526237889525 1103 26-27 0.5067681096973331 1103 28-29 0.6483187718723329 1103 30-31 0.6892429516852303 1103 32-33 0.7710987699061107 1103 34-35 0.5587862068313996 1103 36-37 0.8715381786326972 1103 38-39 0.9008856029776027 1103 40-41 0.7905160457387694 1103 42-43 0.7715600242171661 1103 44-45 0.6341314501631317 1103 46-47 0.7494522983235399 1103 48-49 1.0656655001108533 1103 50-51 1.0370837435560532 1103 52-53 0.91922024444888 1103 54-55 0.8333075714525933 1103 56-57 0.9162121382693797 1103 58-59 0.9711462302612333 1103 60-61 0.9306056393359157 1103 62-63 1.0920972635942405 1103 64-65 0.8974026639809338 1103 66-67 0.841064429810082 1103 68-69 0.97290572340669 1103 70-71 0.9573474808157272 1103 72-73 0.8864230788639844 1103 74-75 0.8727590444659974 1103 76-77 0.6670279760124558 1103 78-79 0.7482924282752315 1103 80-81 0.7519341326732238 1103 82-83 0.689672180442706 1103 84-85 0.8445955921255042 1103 86-87 0.8086567577989037 1103 88-89 0.8344233329280755 1103 90-91 0.9399705939204317 1103 92-93 1.164346560153632 1103 94-95 1.016092717312219 1103 96-97 0.9264067513142749 1103 98-99 0.8799405615120826 1103 100-101 1.027816952062004 1104 1 0.262849866934058 1104 2 0.28490106348504085 1104 3 0.23968892911566897 1104 4 0.17704708832600602 1104 5 0.2433493620401208 1104 6 0.10182340471627782 1104 7 0.08932492153333271 1104 8 0.18865889380621326 1104 9 0.22889784724675621 1104 10-11 0.36874196217011246 1104 12-13 0.3776083107114161 1104 14-15 0.47192948820983105 1104 16-17 0.47016112911173735 1104 18-19 0.3884435868361322 1104 20-21 0.518377959122752 1104 22-23 0.3752876461597765 1104 24-25 0.3641146363764136 1104 26-27 0.3617888543968917 1104 28-29 0.5154415875257925 1104 30-31 0.22960563232233966 1104 32-33 0.3750754233289868 1104 34-35 0.36030812940796153 1104 36-37 0.32247415134813906 1104 38-39 0.4124514761888136 1104 40-41 0.4236029625138471 1104 42-43 0.39975787239409755 1104 44-45 0.5793911105126242 1104 46-47 0.5991715416694134 1104 48-49 0.6372457891302332 1104 50-51 0.4595896329410962 1104 52-53 0.466950633675701 1104 54-55 0.5389360655280484 1104 56-57 0.7034302388987541 1104 58-59 0.7266449820632133 1104 60-61 0.566707393845256 1104 62-63 0.6354304797258763 1104 64-65 0.706667809369641 1104 66-67 0.6680392197260474 1104 68-69 0.6236333701139003 1104 70-71 0.5625821772047388 1104 72-73 0.650995351287591 1104 74-75 0.7055201991370659 1104 76-77 0.7092777210987755 1104 78-79 0.8882628934487471 1104 80-81 0.8500264993269973 1104 82-83 0.8880883753725612 1104 84-85 0.7605591658395099 1104 86-87 0.8220418356794283 1104 88-89 0.7877384413982398 1104 90-91 0.5965250399666324 1104 92-93 0.4834724699317725 1104 94-95 0.7330161728869165 1104 96-97 0.7316604343774564 1104 98-99 0.4424200805163352 1104 100-101 0.6849332630215343 1105 1 0.26880991467739435 1105 2 0.3202262634618087 1105 3 0.3333445737163423 1105 4 0.23343744447108605 1105 5 0.2922967787843902 1105 6 0.215286164723544 1105 7 0.161590875172827 1105 8 0.25542124702074176 1105 9 0.380796994124978 1105 10-11 0.4238373645314155 1105 12-13 0.4061109723838925 1105 14-15 0.4061456111433088 1105 16-17 0.49895441056697365 1105 18-19 0.48999749390150527 1105 20-21 0.43755774053020957 1105 22-23 0.3556521669369417 1105 24-25 0.26974340380002104 1105 26-27 0.2806266750670545 1105 28-29 0.24653378201432474 1105 30-31 0.14193795445122248 1105 32-33 0.41699587482958833 1105 34-35 0.11393188265658694 1105 36-37 0.28129477092478083 1105 38-39 0.3452770837078276 1105 40-41 0.2353021419954544 1105 42-43 0.1337073596403684 1105 44-45 -0.02297900530432173 1105 46-47 0.006283388126227862 1105 48-49 0.17635054528880545 1105 50-51 0.2300710199713265 1105 52-53 0.20210794498363782 1105 54-55 0.14613073206479754 1105 56-57 0.22095305658027797 1105 58-59 0.14660630770914196 1105 60-61 0.017330699265848182 1105 62-63 0.06680130194938982 1105 64-65 0.16347863668976714 1105 66-67 0.18066797028583892 1105 68-69 0.38170804684389736 1105 70-71 0.33153888778603857 1105 72-73 0.326271871045428 1105 74-75 0.48044482724305837 1105 76-77 0.37015816225623155 1105 78-79 0.367691284996603 1105 80-81 0.304236363910551 1105 82-83 0.24684809783160588 1105 84-85 0.3641530326565743 1105 86-87 0.1671216296410556 1105 88-89 0.39003922818899994 1105 90-91 0.3356547170994837 1105 92-93 0.3254345188480805 1105 94-95 0.384497659226291 1105 96-97 0.3050643209251902 1105 98-99 0.08857784714733796 1105 100-101 0.2975449623883897 1106 1 0.20984136376224072 1106 2 0.3296427868716556 1106 3 0.39588274744472685 1106 4 0.4003137717033667 1106 5 0.46538202501757553 1106 6 0.37914095287199245 1106 7 0.4257227388597542 1106 8 0.4103691689542046 1106 9 0.3032016862070961 1106 10-11 0.43034156111348665 1106 12-13 0.4603237340382549 1106 14-15 0.6846854028310503 1106 16-17 0.6074052144905195 1106 18-19 0.45829762536070007 1106 20-21 0.6373918996969579 1106 22-23 0.5172215875052473 1106 24-25 0.5841169501646633 1106 26-27 0.4765878440426832 1106 28-29 0.542284616274614 1106 30-31 0.6569374488389315 1106 32-33 0.4722562651622795 1106 34-35 0.5393459791274182 1106 36-37 0.36449833043535307 1106 38-39 0.3319956599035976 1106 40-41 0.2935426112036694 1106 42-43 0.04752776615265475 1106 44-45 0.23602898336996248 1106 46-47 0.3125357897846399 1106 48-49 0.3041379858793647 1106 50-51 0.21239683653518426 1106 52-53 0.2500551590598832 1106 54-55 0.16533793198390612 1106 56-57 0.3069142255559072 1106 58-59 0.25981795701645183 1106 60-61 0.37920146476286476 1106 62-63 0.35541794670619 1106 64-65 0.282924485612817 1106 66-67 0.19066594783665636 1106 68-69 0.1841865583212936 1106 70-71 0.2588085813849901 1106 72-73 0.23735287013532513 1106 74-75 0.3320759325115361 1106 76-77 0.4116294940390226 1106 78-79 0.41284650797162925 1106 80-81 0.3074844173032041 1106 82-83 0.39178792996832357 1106 84-85 0.4244058388048231 1106 86-87 0.4367687122581074 1106 88-89 0.42466052457892545 1106 90-91 0.5140408026490881 1106 92-93 0.4035495962067621 1106 94-95 0.32789537385870915 1106 96-97 0.4263688006501347 1106 98-99 0.3614396127454782 1106 100-101 0.49575812853259293 1107 1 0.268923729487792 1107 2 0.2503189384423017 1107 3 0.3126822966770142 1107 4 0.3301413128057362 1107 5 0.3699947471313578 1107 6 0.2042124921229913 1107 7 0.19344455078497447 1107 8 0.23331888018113744 1107 9 0.33075446023965327 1107 10-11 0.3548049664220727 1107 12-13 0.4038757433755933 1107 14-15 0.5560604455936087 1107 16-17 0.41878126343102196 1107 18-19 0.40335548059182 1107 20-21 0.4810394959378783 1107 22-23 0.4363486275310038 1107 24-25 0.513789539865428 1107 26-27 0.2607622750490606 1107 28-29 0.4469037779271545 1107 30-31 0.5261482028686686 1107 32-33 0.6113505037874418 1107 34-35 0.4421825959547334 1107 36-37 0.6144932995456927 1107 38-39 0.5771505106874102 1107 40-41 0.4125436777522751 1107 42-43 0.5973398121148605 1107 44-45 0.5081335051335927 1107 46-47 0.6163751635394945 1107 48-49 0.6945585682551467 1107 50-51 0.6170767713348511 1107 52-53 0.685797617756279 1107 54-55 0.790669870083839 1107 56-57 0.6873020065310911 1107 58-59 0.5973815977706849 1107 60-61 0.8060990157257777 1107 62-63 0.7118301174344595 1107 64-65 0.8219158928532764 1107 66-67 0.8514453332631646 1107 68-69 0.7877490319091294 1107 70-71 0.6605796557817811 1107 72-73 0.787443224913666 1107 74-75 0.7400174936972199 1107 76-77 0.7536440634954822 1107 78-79 0.8225062185948531 1107 80-81 0.7508257329814718 1107 82-83 0.6520847524584141 1107 84-85 0.7448054926796104 1107 86-87 0.8760684124170801 1107 88-89 0.7533468586702483 1107 90-91 0.7182985451882793 1107 92-93 0.6531308249512549 1107 94-95 0.5906301654792578 1107 96-97 0.37587355987175997 1107 98-99 0.17691241575596095 1107 100-101 0.19656286964301017 1108 1 -0.10166201394092411 1108 2 0.1251234600139206 1108 3 0.023531146533347425 1108 4 0.21409959734193507 1108 5 0.18615110204520136 1108 6 0.09072987240671182 1108 7 0.018403715795436426 1108 8 0.16045750888304866 1108 9 0.22403232137517648 1108 10-11 0.08604927986473143 1108 12-13 0.26476351498720874 1108 14-15 0.3071645170333923 1108 16-17 0.2687550965707075 1108 18-19 0.40899874859690044 1108 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12 5618.0 13 7414.0 14 8483.0 15 11361.0 16 15303.0 17 21222.0 18 18451.0 19 9831.0 20 9096.0 21 9484.0 22 10269.0 23 10843.0 24 12184.0 25 14123.0 26 16316.0 27 19201.0 28 22790.0 29 27888.0 30 33619.0 31 42462.0 32 54267.0 33 71193.0 34 99453.0 35 144875.0 36 218517.0 37 311709.0 38 261707.0 39 41242.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.841558985171346 18.863317494399528 14.974252043170786 21.32087147725834 2 15.257735953858772 20.831433387802026 40.57715285896053 23.33367779937867 3 18.750754878208674 26.87301834378268 30.259716856890595 24.116509921118055 4 12.41203034133681 16.975051659465453 37.98797120893161 32.62494679026612 5 13.099570433856636 38.10176737261632 33.46099270616332 15.337669487363726 6 29.828203204900838 36.68896258407809 18.190005666044502 15.292828544976569 7 27.197261335744738 34.1962278775352 21.172652112130216 17.433858674589846 8 26.641417078232372 33.22407782145414 20.155994386683105 19.978510713630385 9 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25.989426825716343 31.34237077536396 21.605841207351624 34-35 21.534059132528316 25.70315295126623 31.368448788275273 21.39433912793018 36-37 21.66578944819191 25.53687385167796 31.194351789364237 21.602984910765894 38-39 21.43079023437601 25.830409983648185 31.245366340060915 21.493433441914895 40-41 21.431150037392136 25.845068694323498 31.28846578587417 21.435315482410196 42-43 21.348390071645653 26.0009015443791 30.689997894674303 21.96071048930094 44-45 21.330817276193777 26.184689314409948 30.45769184693512 22.026801562461152 46-47 21.821493036607457 26.26905528413806 30.034066214387888 21.875385464866593 48-49 21.841030502800223 26.67819649093802 29.294848777614746 22.185924228647007 50-51 21.827268893534384 26.835493230986025 29.194299084311893 22.1429387911677 52-53 21.998351890708136 27.162094002935856 28.767999555682135 22.071554550673874 54-55 21.698765422531096 26.97981076894611 28.903669504066876 22.41775430445592 56-57 21.858137354907186 27.222410044634998 28.53062274915827 22.388829851299544 58-59 22.073430749752088 26.733932280810635 28.80201570808269 22.390621261354585 60-61 21.938640084474024 26.931907349821266 29.152418133498664 21.977034432206043 62-63 21.952973459780225 26.908594451065877 29.281459497353275 21.856972591800627 64-65 21.820792076650246 27.081455094043278 29.088383375225064 22.00936945408141 66-67 21.830887215947257 27.2520448180719 28.661296489578277 22.25577147640257 68-69 21.676543249745684 27.184318031357478 29.02262195023494 22.1165167686619 70-71 21.881553537913263 27.112498074210635 28.760489294226055 22.245459093650048 72-73 21.763928898993683 27.254847502292957 28.451512059009705 22.52971153970366 74-75 21.543931424747626 27.680876529278898 28.3941901450526 22.38100190092088 76-77 21.743889751475052 27.32304754509628 28.563944228447983 22.369118474980677 78-79 21.592273972585044 27.386826830007955 28.513576840262 22.507322357145 80-81 21.759517547784395 27.730729483488876 28.102395753345398 22.407357215381328 82-83 21.738412777987378 27.343414871129625 28.355649416762713 22.562522934120285 84-85 21.60977591637105 27.427185093129186 28.19483235712828 22.768206633371484 86-87 21.738036639230465 27.40772939402561 28.158508414454047 22.69572555228988 88-89 21.79041267183895 27.261776506666873 27.945709096452 23.002101725042177 90-91 21.526573574509523 27.190812007564073 28.380619562917953 22.901994855008454 92-93 21.708989100108074 27.244141946011524 28.305158214130905 22.741710739749497 94-95 21.407575386678257 27.009380261985942 28.372886566842197 23.2101577844936 96-97 21.38938248016861 26.9538302145039 28.388775960371454 23.268011344956037 98-99 21.21237373125651 27.28309230235056 28.274347359156348 23.230186607236583 100-101 21.2353653352422 27.163430167727697 28.40533353662397 23.195870960406133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 815.0 1 615.0 2 521.5 3 634.0 4 562.5 5 590.5 6 1090.0 7 2404.5 8 4065.5 9 4790.0 10 4107.5 11 3275.0 12 3232.5 13 3469.5 14 3665.5 15 3756.5 16 3833.5 17 3903.0 18 3945.0 19 4071.5 20 4353.5 21 4886.0 22 5508.5 23 6353.0 24 7285.0 25 8371.5 26 9898.5 27 11592.5 28 12874.0 29 14699.0 30 17793.5 31 20766.0 32 24137.0 33 27495.5 34 30642.5 35 33631.5 36 37909.5 37 42137.5 38 44032.0 39 44791.0 40 44863.0 41 45993.5 42 46693.0 43 48337.0 44 51671.5 45 53913.5 46 53597.0 47 54190.5 48 61205.0 49 61401.5 50 53834.0 51 50483.0 52 48666.5 53 48349.5 54 47894.0 55 45048.5 56 42016.5 57 38910.0 58 36283.5 59 33014.0 60 28253.0 61 23763.0 62 19414.0 63 14332.5 64 10015.5 65 7265.5 66 5112.5 67 3871.5 68 2743.0 69 1708.5 70 1132.0 71 707.5 72 468.5 73 361.0 74 251.0 75 198.0 76 150.0 77 73.5 78 43.0 79 25.5 80 14.5 81 13.5 82 12.5 83 14.0 84 12.5 85 6.0 86 5.0 87 5.5 88 6.0 89 4.5 90 2.5 91 2.0 92 1.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02395936594863913 2 0.01065578269952953 3 0.012399456232179817 4 0.021699048406314682 5 0.0546996845242516 6 0.04120235977151419 7 0.01646802780836382 8 0.04565841435495381 9 0.02828625952966021 10-11 0.023475012189569603 12-13 0.03655256368444676 14-15 0.018857506353106807 16-17 0.01666176931199163 18-19 0.01685551081561944 20-21 0.013077551494877153 22-23 0.007943401648740196 24-25 0.001743673532650287 26-27 1.6145125302317471E-4 28-29 0.0026155102989754305 30-31 0.0 32-33 5.166440096741591E-4 34-35 6.458050120926988E-5 36-37 0.0 38-39 1.9374150362780964E-4 40-41 6.458050120926988E-5 42-43 6.458050120926988E-5 44-45 3.229025060463494E-5 46-47 0.0 48-49 4.5206350846488917E-4 50-51 1.2916100241853977E-4 52-53 9.041270169297783E-4 54-55 0.0019697052868827315 56-57 4.197732578602543E-4 58-59 0.0010655782699529532 60-61 0.003229025060463494 62-63 0.0020342857880920016 64-65 0.0012270295229761278 66-67 0.009751655682599752 68-69 0.011624490217668579 70-71 0.025089524719801352 72-73 0.015176417784178421 74-75 0.01666176931199163 76-77 0.029803901308078053 78-79 0.01843773309524655 80-81 0.0449157385910472 82-83 0.03506721215663355 84-85 0.01827628184222338 86-87 0.012690068487621534 88-89 0.013109841745481788 90-91 0.022441724170221285 92-93 0.029577869553845604 94-95 0.013174422246691055 96-97 0.017953379336177026 98-99 0.026962359254870175 100-101 0.038037915212259965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.88132393052225 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35764426741441 35.85818772215503 2 14.004904914721175 13.691565875052977 3 5.066410381959165 7.429585411365207 4 2.4054319984744144 4.703228028410854 5 1.3153449170825193 3.2147900486138283 6 0.7959628264418324 2.3344630053574367 7 0.5646501814974922 1.9320593900444973 8 0.4067411792871755 1.5905637872495242 9 0.2931375689453427 1.2896057217440788 >10 1.590412467868664 14.142836363403477 >50 0.12874960369461916 4.344940504538385 >100 0.06624625258698197 5.791494471650735 >500 0.002908960017364813 0.9764190872979769 >1k 0.0010578036426781138 0.7659076047184104 >5k 2.6445091066952845E-4 1.036592925272056 >10k+ 1.3222545533476423E-4 0.8977600531256303 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13890 0.8970231617967587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9477 0.6120294099602507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6561 0.4237126684340197 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2193 0.14162503915192887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2171 0.1402042681253249 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.10352254343845962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9374150362780964E-4 0.0 14-15 0.0 0.0 0.0 2.5832200483707953E-4 0.0 16-17 0.0 0.0 0.0 2.5832200483707953E-4 0.0 18-19 0.0 0.0 0.0 2.906122554417145E-4 0.0 20-21 0.0 0.0 0.0 3.8748300725561927E-4 0.0 22-23 0.0 0.0 0.0 5.81224510883429E-4 0.0 24-25 0.0 0.0 0.0 0.001097868520557588 0.0 26-27 0.0 0.0 0.0 0.0014853515278132074 0.0 28-29 0.0 0.0 0.0 0.003422766564091304 0.0 30-31 0.0 0.0 0.0 0.007846530896926291 0.0 32-33 0.0 0.0 0.0 0.014207710266039375 0.0 34-35 0.0 0.0 0.0 0.01947102111459487 0.0 36-37 0.0 0.0 0.0 0.02370104394380205 0.0 38-39 0.0 0.0 0.0 0.025993651736731127 0.0 40-41 0.0 0.0 0.0 0.030804899076821737 0.0 42-43 0.0 0.0 0.0 0.03606820992537723 0.0 44-45 0.0 0.0 0.0 0.042849162552350564 0.0 46-47 0.0 0.0 0.0 0.05050195194564905 0.0 48-49 0.0 0.0 0.0 0.05986612462099318 0.0 50-51 0.0 0.0 0.0 0.07090939032777833 0.0 52-53 0.0 0.0 0.0 0.09699991281632336 0.0 54-55 0.0 0.0 0.0 0.1256736553532392 0.0 56-57 0.0 0.0 0.0 0.16290431430038327 0.0 58-59 0.0 0.0 0.0 0.22021950912361032 0.0 60-61 0.0 0.0 0.0 0.29900772059891956 0.0 62-63 0.0 0.0 0.0 0.3527064073544275 0.0 64-65 0.0 0.0 0.0 0.40272400554100696 0.0 66-67 0.0 0.0 0.0 0.506924644242164 0.0 68-69 0.0 0.0 0.0 0.6386688667090745 0.0 70-71 0.0 0.0 0.0 0.7394790290967448 0.0 72-73 0.0 0.0 0.0 0.833572819358651 0.0 74-75 0.0 0.0 0.0 1.0258612617092522 0.0 76-77 0.0 0.0 0.0 1.1632885682825784 0.0 78-79 0.0 0.0 0.0 1.2677475289885725 0.0 80-81 0.0 0.0 0.0 1.3862527487075826 0.0 82-83 0.0 0.0 0.0 1.5940405113484086 0.0 84-85 0.0 0.0 0.0 1.8060582968184415 0.0 86-87 0.0 0.0 0.0 2.117659215153169 0.0 88-89 0.0 0.0 0.0 2.529424490863474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13595 0.0 35.062134 1 GGTATCA 8435 0.0 33.41075 1 ATCAACG 16585 0.0 28.594965 3 TATCAAC 16655 0.0 28.503317 2 TCAACGC 16715 0.0 28.401 4 CAACGCA 16870 0.0 28.168224 5 AACGCAG 17275 0.0 27.56018 6 GTACATG 10490 0.0 24.917463 1 ACGCAGA 19065 0.0 24.845554 7 GAGTACT 9250 0.0 24.779745 12-13 CGCAGAG 19025 0.0 24.697212 8 TACATGG 10635 0.0 24.173206 2 GTACTTT 9705 0.0 23.470388 14-15 GCAGAGT 19495 0.0 23.44305 9 GTTCGGG 125 2.2485618E-4 22.811712 1 ACATGGG 10675 0.0 22.791693 3 AGTACTT 9565 0.0 22.52338 12-13 CATGGGG 3515 0.0 22.30659 4 AGAGTAC 15975 0.0 21.158716 10-11 ACGTAAC 135 3.7836214E-4 21.121956 1 >>END_MODULE