FastQCFastQC Report
Thu 26 May 2016
SRR937089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548455
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140950.910262164544659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105290.6799680972324027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72480.4680794727647881No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27810.17959837386297955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26390.17042794269126324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.12515701134356505No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.1037808654432967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15850.10236009441669278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94550.033.5647621
GTATCAA146550.033.033681
TATCAAC182500.026.4675942
TCAACGC184100.026.2367214
ATCAACG184300.026.2082483
CAACGCA187450.025.7981685
AACGCAG192700.025.1199636
GTACATG106100.024.895911
GAGTACT110450.023.95222712-13
CGTATAG1601.8574592E-623.75411
TACATGG109750.023.5856782
ACATGGG108100.022.7147123
ACGCAGA213200.022.5263337
GTACTTT117300.022.43273414-15
AGTACTT113350.022.31282212-13
CGCAGAG214650.022.2413778
GGACCGT1954.22031E-721.924746
TAAGGTG6150.021.6276575
GCAGAGT220900.021.2895289
CATGGGA69500.020.9112034
Produced by FastQC (version 0.11.5)