##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937089_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548455 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.224670397266955 33.0 31.0 34.0 31.0 34.0 2 32.3344617699578 33.0 31.0 34.0 31.0 34.0 3 32.42605241999283 34.0 31.0 34.0 31.0 34.0 4 36.08415291371076 37.0 35.0 37.0 35.0 37.0 5 36.00532659973974 37.0 35.0 37.0 35.0 37.0 6 36.06100790788237 37.0 35.0 37.0 35.0 37.0 7 36.005501612898016 37.0 35.0 37.0 35.0 37.0 8 35.97295368609356 37.0 35.0 37.0 35.0 37.0 9 37.64852062216855 39.0 37.0 39.0 35.0 39.0 10-11 37.567623857328755 39.0 37.0 39.0 35.0 39.0 12-13 37.47192491870929 39.0 37.0 39.0 35.0 39.0 14-15 38.63137546780501 40.0 38.0 41.0 34.0 41.0 16-17 38.56424403679797 40.0 38.0 41.0 34.0 41.0 18-19 38.760009170431175 40.0 38.0 41.0 35.0 41.0 20-21 38.79901676186908 40.0 38.0 41.0 35.0 41.0 22-23 38.71253507528472 40.0 38.0 41.0 34.5 41.0 24-25 38.597709652524614 40.0 38.0 41.0 34.0 41.0 26-27 38.404406650500015 40.0 38.0 41.0 34.0 41.0 28-29 38.309311216664355 40.0 38.0 41.0 34.0 41.0 30-31 38.2392949746683 40.0 37.5 41.0 34.0 41.0 32-33 38.01048270695629 40.0 37.0 41.0 33.5 41.0 34-35 37.832310916365024 40.0 37.0 41.0 33.0 41.0 36-37 37.64201381376921 40.0 36.5 41.0 33.0 41.0 38-39 37.454387437800904 40.0 36.0 41.0 33.0 41.0 40-41 37.23371780258387 40.0 36.0 41.0 32.5 41.0 42-43 36.919569183476426 39.5 36.0 41.0 31.5 41.0 44-45 36.54368806326306 39.0 35.0 41.0 31.0 41.0 46-47 36.03137127007243 39.0 35.0 41.0 29.5 41.0 48-49 35.53585541717389 39.0 35.0 40.5 27.5 41.0 50-51 35.18444901530881 39.0 35.0 40.0 26.5 41.0 52-53 34.91629721238266 38.5 35.0 40.0 26.0 41.0 54-55 34.61918525239675 38.0 34.0 40.0 24.5 41.0 56-57 34.319370275532705 38.0 34.0 40.0 23.5 41.0 58-59 33.956900910907976 38.0 34.0 40.0 23.0 41.0 60-61 33.615689509866286 37.0 33.0 40.0 22.0 41.0 62-63 33.22896596930489 36.5 33.0 39.5 21.5 41.0 64-65 32.82754003183818 36.0 32.5 39.0 20.0 40.0 66-67 32.42563232383247 35.5 32.0 39.0 19.0 40.0 68-69 32.116057941625684 35.0 32.0 38.5 18.0 40.0 70-71 31.64883900403951 35.0 31.0 37.5 15.0 40.0 72-73 31.163574982805443 35.0 31.0 37.0 9.5 39.0 74-75 30.659455069730797 34.5 30.0 36.5 7.5 39.0 76-77 29.275667681656877 33.0 28.5 35.0 7.0 37.0 78-79 30.010925729194582 34.0 30.0 35.0 4.5 37.5 80-81 29.7793058241925 34.0 30.0 35.0 2.0 37.0 82-83 29.47334956456597 34.0 29.5 35.0 2.0 37.0 84-85 29.13273747057551 34.0 29.0 35.0 2.0 36.0 86-87 28.798102301971966 34.0 29.0 35.0 2.0 36.0 88-89 28.41314213199609 34.0 29.0 35.0 2.0 35.0 90-91 27.97019448417939 33.5 28.0 35.0 2.0 35.0 92-93 27.503851903994626 33.0 27.0 35.0 2.0 35.0 94-95 26.93681637503189 33.0 25.5 35.0 2.0 35.0 96-97 26.281797017026648 32.5 25.0 34.0 2.0 35.0 98-99 25.49532404880994 32.0 22.0 34.0 2.0 35.0 100-101 22.062556225398865 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13283929031094743 1101 2 0.057164932288642945 1101 3 0.13378636270471134 1101 4 0.15254716224357168 1101 5 0.23104731872934536 1101 6 0.20747129747044823 1101 7 0.16411277137383706 1101 8 0.253942756719006 1101 9 0.22146585707678668 1101 10-11 0.22960451515043445 1101 12-13 0.2528768890628328 1101 14-15 0.20590670939287037 1101 16-17 0.1696386298408612 1101 18-19 0.21756612330155178 1101 20-21 0.2429977018024303 1101 22-23 0.27449672817721904 1101 24-25 0.25955595989748304 1101 26-27 0.172775776317728 1101 28-29 0.20947771625265688 1101 30-31 0.32686865164189527 1101 32-33 0.38698188795658695 1101 34-35 0.29355447487314734 1101 36-37 0.2812187756875204 1101 38-39 0.3028546132447403 1101 40-41 0.33231366432305975 1101 42-43 0.32613253520263186 1101 44-45 0.28873982745599136 1101 46-47 0.35156332521678735 1101 48-49 0.28323497380217333 1101 50-51 0.1805830549364913 1101 52-53 0.1829936566006083 1101 54-55 0.08058765162788006 1101 56-57 0.13642726584632925 1101 58-59 0.11569765731934467 1101 60-61 0.20760127708299336 1101 62-63 -0.08069490007346047 1101 64-65 -0.1088141038254804 1101 66-67 -0.23348348478271674 1101 68-69 -0.3028671449034839 1101 70-71 -0.17600602063683013 1101 72-73 0.13011706114046362 1101 74-75 0.09489765209027823 1101 76-77 -0.11351124855241679 1101 78-79 -0.04089757185935028 1101 80-81 0.12311311147290382 1101 82-83 0.006942442658086634 1101 84-85 0.11132159116149509 1101 86-87 -0.34091255700431944 1101 88-89 -0.23775452730325242 1101 90-91 0.02952025243111933 1101 92-93 0.3387287452904282 1101 94-95 0.1446209236122975 1101 96-97 0.09737597336686221 1101 98-99 0.273517704038607 1101 100-101 0.8791584165328636 1102 1 0.26833082002263353 1102 2 0.30875438845818337 1102 3 0.319185940406598 1102 4 0.24823855378398463 1102 5 0.21358324812577223 1102 6 0.25176784026948695 1102 7 0.15964458482315536 1102 8 0.2394026627236201 1102 9 0.32564407855949895 1102 10-11 0.2687502858444972 1102 12-13 0.31371165762595155 1102 14-15 0.4764154132100913 1102 16-17 0.4351099203800217 1102 18-19 0.3720313907408084 1102 20-21 0.4342744238349141 1102 22-23 0.2469768052417578 1102 24-25 0.3722706362166832 1102 26-27 0.2173997461146584 1102 28-29 0.40347553664945934 1102 30-31 0.46644304519650603 1102 32-33 0.38012197611374887 1102 34-35 0.4019162695428449 1102 36-37 0.4440224849051049 1102 38-39 0.5966072282820534 1102 40-41 0.412841245060811 1102 42-43 0.34206212623512045 1102 44-45 0.2753119149125709 1102 46-47 0.3227935360739309 1102 48-49 0.279358880351694 1102 50-51 0.2346060769952345 1102 52-53 0.34180596746650593 1102 54-55 0.4172769900599249 1102 56-57 0.18572375999350754 1102 58-59 0.2917327255779014 1102 60-61 0.41829057659355584 1102 62-63 0.4127302879659709 1102 64-65 0.44766632353243097 1102 66-67 0.2829433282287397 1102 68-69 0.12705082806554202 1102 70-71 0.22991101821654425 1102 72-73 0.49556159315825354 1102 74-75 0.42064713394799824 1102 76-77 0.242940474209842 1102 78-79 0.1938437462167606 1102 80-81 0.35400606765704623 1102 82-83 0.23378415870638491 1102 84-85 0.40834166491770674 1102 86-87 0.3394661490508888 1102 88-89 0.3886867839615995 1102 90-91 0.47224193461772757 1102 92-93 0.423573205303466 1102 94-95 0.4318853269945713 1102 96-97 0.5716556826233621 1102 98-99 0.49943989998882543 1102 100-101 0.8105656228459317 1103 1 0.2745984498815943 1103 2 0.3046883409865586 1103 3 0.21638236283521195 1103 4 0.17540057648780305 1103 5 0.20469091926440086 1103 6 0.1693495396158866 1103 7 0.1169338865087397 1103 8 0.20993929843053394 1103 9 0.27943492823270333 1103 10-11 0.3023226972652111 1103 12-13 0.276386236229321 1103 14-15 0.4509269371178988 1103 16-17 0.4308116747659909 1103 18-19 0.30554188265687543 1103 20-21 0.31046809428554667 1103 22-23 0.37570745539655803 1103 24-25 0.24428990006256868 1103 26-27 0.30940525007062547 1103 28-29 0.4383602975713714 1103 30-31 0.4692294229054781 1103 32-33 0.4801271360724755 1103 34-35 0.5303682819267479 1103 36-37 0.2880696125280764 1103 38-39 0.2610275929191346 1103 40-41 0.36695201217467144 1103 42-43 0.3589609910442064 1103 44-45 0.4213232668217586 1103 46-47 0.3915998656232915 1103 48-49 0.5188188046722999 1103 50-51 0.509334319169298 1103 52-53 0.5738166313811988 1103 54-55 0.5607874819759928 1103 56-57 0.49680735132477594 1103 58-59 0.46976506131921525 1103 60-61 0.509857484058287 1103 62-63 0.5722366519150626 1103 64-65 0.5553598219844389 1103 66-67 0.5363884606676805 1103 68-69 0.483397577291548 1103 70-71 0.5032391915911596 1103 72-73 0.4830882529449738 1103 74-75 0.6280207149593515 1103 76-77 0.5380247535356055 1103 78-79 0.5506583317000775 1103 80-81 0.6731237147158673 1103 82-83 0.6407415030476322 1103 84-85 0.7292257378451232 1103 86-87 0.5226016839666094 1103 88-89 0.350823006871817 1103 90-91 0.46342012519220077 1103 92-93 0.4456905083650433 1103 94-95 0.20626785193437414 1103 96-97 0.40471553814451866 1103 98-99 0.5484302680658821 1103 100-101 0.7203712644007965 1104 1 0.15728682230169255 1104 2 0.18055627811957464 1104 3 0.10744983584952195 1104 4 0.04127449090712787 1104 5 0.015715496459293377 1104 6 -0.029350576413783358 1104 7 0.02464739869186161 1104 8 0.1288679577603702 1104 9 0.173190070456954 1104 10-11 0.1610817071453141 1104 12-13 0.06799922452086804 1104 14-15 0.07562490835660896 1104 16-17 0.1487166359136296 1104 18-19 0.23340676082571576 1104 20-21 0.16709461937256975 1104 22-23 0.09997488621825568 1104 24-25 0.11219960787875038 1104 26-27 -0.038807892927160026 1104 28-29 -0.05786627674036282 1104 30-31 0.1926392889439441 1104 32-33 0.18824059903087687 1104 34-35 0.05607834838011172 1104 36-37 0.07905322773148526 1104 38-39 0.04164852748542813 1104 40-41 0.07432165459231754 1104 42-43 0.12732444521248 1104 44-45 0.020118492024806756 1104 46-47 0.05022438656138206 1104 48-49 -0.11421378911835944 1104 50-51 0.07476784822945604 1104 52-53 -0.0021288346713319584 1104 54-55 0.05835947311554435 1104 56-57 0.010030866672344985 1104 58-59 0.03089793287788467 1104 60-61 0.06399042593796622 1104 62-63 0.1428229533812555 1104 64-65 -0.09256553225767306 1104 66-67 0.009494364116925169 1104 68-69 -0.1006114237984228 1104 70-71 -0.18002071488189486 1104 72-73 0.04483994943947067 1104 74-75 0.14666615639952596 1104 76-77 7.1446689855037E-5 1104 78-79 -0.04144391505636591 1104 80-81 0.4425894508010977 1104 82-83 0.5516333493258898 1104 84-85 0.3189877012078739 1104 86-87 0.054392936032289896 1104 88-89 0.10939021618231592 1104 90-91 0.20554188603043144 1104 92-93 -0.059500413191866386 1104 94-95 0.05765704359430046 1104 96-97 0.12724234803165402 1104 98-99 -0.07686050933294197 1104 100-101 0.2734332172153948 1105 1 0.2667835872594324 1105 2 0.1871297940568155 1105 3 0.2666405225254209 1105 4 0.12693293864897015 1105 5 0.15317884107889768 1105 6 0.20488522097307538 1105 7 0.23989903495056097 1105 8 0.17168556433501436 1105 9 0.23135637392697106 1105 10-11 0.28589175498234454 1105 12-13 0.26310853016256175 1105 14-15 0.3163529006067449 1105 16-17 0.23101543652147427 1105 18-19 0.21537315685935 1105 20-21 0.3536075005182582 1105 22-23 0.28204527579116956 1105 24-25 0.27006161313651944 1105 26-27 0.359379434101605 1105 28-29 0.35033890719703464 1105 30-31 0.3037306215040445 1105 32-33 0.2775206452002621 1105 34-35 0.30347541819818247 1105 36-37 0.24550922160054967 1105 38-39 0.25752768511902246 1105 40-41 0.1644810551398237 1105 42-43 0.27881623721791016 1105 44-45 0.3431940025768725 1105 46-47 0.24201845312099124 1105 48-49 0.11678303111522581 1105 50-51 0.09146321461462747 1105 52-53 0.08541629946159901 1105 54-55 0.1632833329809671 1105 56-57 0.20574520859934609 1105 58-59 0.21620430159212134 1105 60-61 0.2961169345469514 1105 62-63 0.36294935705981857 1105 64-65 0.09995967446461407 1105 66-67 0.08990404244897832 1105 68-69 0.06847963519308564 1105 70-71 -0.1529630949661005 1105 72-73 0.1338977679735578 1105 74-75 0.22340795449466455 1105 76-77 0.09961612362596028 1105 78-79 0.23473725480537055 1105 80-81 0.4518117102902721 1105 82-83 0.5416312319799559 1105 84-85 0.4227828095766384 1105 86-87 0.23385468046301483 1105 88-89 0.256249892359552 1105 90-91 0.29546050874374075 1105 92-93 0.4583025312306148 1105 94-95 0.2308999743758271 1105 96-97 0.3005398051553989 1105 98-99 0.26041901967937875 1105 100-101 0.5401582826563747 1106 1 0.261543421418601 1106 2 0.2006276199239423 1106 3 0.17063283721851974 1106 4 0.1758559844574421 1106 5 0.19624689649400295 1106 6 0.18094346750962842 1106 7 0.20046329827344778 1106 8 0.2417229796449547 1106 9 0.20184479540537836 1106 10-11 0.23466615077563802 1106 12-13 0.2345171660205878 1106 14-15 0.33106925210841354 1106 16-17 0.21903748121759747 1106 18-19 0.020409055332393677 1106 20-21 0.23135044722672404 1106 22-23 0.25954047247435597 1106 24-25 0.20673771789938655 1106 26-27 0.24807697682584973 1106 28-29 0.23016295411785848 1106 30-31 0.3556620604766394 1106 32-33 0.3192447831312961 1106 34-35 0.16378384169905047 1106 36-37 0.20375272448254123 1106 38-39 0.3319194335263447 1106 40-41 0.33562373892989683 1106 42-43 0.19570672159449032 1106 44-45 0.06983370325438898 1106 46-47 0.12281428687566631 1106 48-49 0.15859110068748095 1106 50-51 0.14757910095298143 1106 52-53 0.3999931019694358 1106 54-55 0.24146110626440986 1106 56-57 0.37699710464546143 1106 58-59 0.6620421011674154 1106 60-61 0.3986905011360946 1106 62-63 0.6198742230725571 1106 64-65 0.3681586834645145 1106 66-67 0.5482385555443443 1106 68-69 0.49810345964449 1106 70-71 0.3857344477581357 1106 72-73 0.2850616874800771 1106 74-75 0.47875126524398226 1106 76-77 0.5063549243135945 1106 78-79 0.44826743985947104 1106 80-81 0.4578770353989796 1106 82-83 0.445551749726949 1106 84-85 0.3441782995149545 1106 86-87 0.26900585853966774 1106 88-89 0.4177869537408867 1106 90-91 0.1438850208278737 1106 92-93 0.4268574912872438 1106 94-95 0.35911415174462036 1106 96-97 0.4040608891881625 1106 98-99 0.13946442366360756 1106 100-101 0.34554204238941466 1107 1 0.21768090557129938 1107 2 0.21027565888315536 1107 3 0.18656143883242748 1107 4 0.2095468463494612 1107 5 0.17499843091535183 1107 6 0.12133185219157383 1107 7 0.17579704749365987 1107 8 0.202884511450236 1107 9 0.2027841631231624 1107 10-11 0.2664933699010703 1107 12-13 0.30139137365546986 1107 14-15 0.2878094368426787 1107 16-17 0.24060892991258953 1107 18-19 0.2774311711233537 1107 20-21 0.1624171970707664 1107 22-23 0.2800151872229151 1107 24-25 0.3503931623281744 1107 26-27 0.480175863993054 1107 28-29 0.46542425852693015 1107 30-31 0.4103508496421142 1107 32-33 0.47247622417933144 1107 34-35 0.5054671773801331 1107 36-37 0.47338446335924544 1107 38-39 0.4504166061663568 1107 40-41 0.5395254957165676 1107 42-43 0.6289244786226433 1107 44-45 0.4578424771259648 1107 46-47 0.46988469920738396 1107 48-49 0.648121215593001 1107 50-51 0.547055687192497 1107 52-53 0.5320891718384289 1107 54-55 0.6626331023201999 1107 56-57 0.8500784239459662 1107 58-59 0.7248008398227554 1107 60-61 0.6671377673506456 1107 62-63 0.7303040622853203 1107 64-65 0.7690916116435318 1107 66-67 0.8182003207829069 1107 68-69 0.6065563604554711 1107 70-71 0.6539843018623408 1107 72-73 0.547345711386864 1107 74-75 0.387194506746809 1107 76-77 0.6494801129719576 1107 78-79 0.8260691423125337 1107 80-81 0.9399075024667738 1107 82-83 0.797760511524384 1107 84-85 0.7024971774253252 1107 86-87 0.6173110524301535 1107 88-89 0.7552855954006752 1107 90-91 0.9125608906686971 1107 92-93 1.0555218906996338 1107 94-95 1.3049783579708496 1107 96-97 1.1327341429663598 1107 98-99 1.0748203087983335 1107 100-101 1.2791025036213775 1108 1 0.18511379356554158 1108 2 0.1082848130326255 1108 3 0.11562064550887641 1108 4 0.1648954376292906 1108 5 0.17769551143584295 1108 6 0.15123087207452102 1108 7 0.17349923383726207 1108 8 0.1448288676411167 1108 9 0.14947720536660825 1108 10-11 0.15381952648016295 1108 12-13 0.13988831364020626 1108 14-15 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-1.0611225075470436 2316 76-77 -0.8400612805929413 2316 78-79 -0.9592900981351491 2316 80-81 -1.1266069045453762 2316 82-83 -0.9186721483715949 2316 84-85 -1.2091994854159545 2316 86-87 -1.2189846226871985 2316 88-89 -1.3848061072114497 2316 90-91 -1.3799392727366069 2316 92-93 -1.3590340314865337 2316 94-95 -1.2890170417807774 2316 96-97 -1.0377927424307174 2316 98-99 -1.592500720152639 2316 100-101 -1.6078545961501227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 14.0 5 40.0 6 84.0 7 172.0 8 479.0 9 957.0 10 1791.0 11 2817.0 12 4265.0 13 6439.0 14 8206.0 15 11499.0 16 16349.0 17 23413.0 18 20816.0 19 11241.0 20 8981.0 21 9094.0 22 9277.0 23 10047.0 24 11347.0 25 13290.0 26 15994.0 27 19552.0 28 24438.0 29 30439.0 30 37528.0 31 46803.0 32 59850.0 33 78895.0 34 107699.0 35 155042.0 36 237724.0 37 338364.0 38 213981.0 39 11523.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21739484322574 19.6997200298187 15.291449346327118 20.79143578062844 2 15.670451316231368 21.74177839778863 39.53415226433129 23.05361802164871 3 19.319488108123466 27.01891954028927 30.192928163094496 23.468664188492767 4 12.99271788857517 17.5798151960797 37.14109316917799 32.28637374616714 5 13.226667872169356 38.70380476022874 32.9030549806097 15.166472386992194 6 30.239561369235783 37.147673003090176 17.38991446312615 15.222851164547887 7 27.556370704993043 34.5659383062472 20.626107959223873 17.25158302953589 8 27.27228108017346 33.368292911321284 19.632278626114417 19.727147382390836 9 27.77397828671584 16.492008475555924 20.43090749283317 35.30310574489506 10-11 24.85270485683209 27.50346842762031 26.389437895123013 21.254388820424584 12-13 26.844435259662053 25.107897872395384 27.39043110713582 20.65723576080674 14-15 23.595261082821263 25.493572625617144 27.155228921731663 23.755937369829926 16-17 21.316021453642502 29.111695205866496 27.354653509465887 22.217629831025118 18-19 21.27608459440827 26.62402180248281 31.40284058710418 20.69705301600474 20-21 22.61625641007426 25.418848564831155 31.502417107782243 20.462477917312345 22-23 22.16278895546563 25.559053023013107 31.55644908171028 20.72170893981098 24-25 21.900046051310074 25.721852670685337 31.72680141034559 20.651299867659 26-27 21.584938732304767 25.91039345272348 31.745630402055326 20.75903741291643 28-29 21.236118793517267 25.970558328756184 31.917241192249158 20.876081685477395 30-31 21.459598608122384 25.778604144116684 31.725535580969407 21.036261666791525 32-33 21.26404248565357 26.123503800581442 31.50696388454654 21.105489829218445 34-35 21.68865094561999 25.780503792489935 31.554646405610754 20.976198856279325 36-37 21.91634220198092 25.618904883649662 31.326932199717177 21.137820714652243 38-39 21.5800210753873 26.006950421168657 31.368676213134624 21.044352290309423 40-41 21.6638605967726 26.063319661411665 31.3187753523087 20.954044389507043 42-43 21.642411306754152 26.270766667420105 30.687556306124492 21.399265719701248 44-45 21.626815115712112 26.371705990810195 30.368593210651905 21.632885682825783 46-47 22.238105463357773 26.56665283565366 29.804595232982606 21.39064646800596 48-49 22.22305462457452 27.046657622902142 29.08047131338775 21.649816439135584 50-51 22.17095938646491 27.29932763410585 28.904241546664377 21.625471432764865 52-53 22.417667933520832 27.494082811576703 28.4819707385749 21.606278516327563 54-55 22.031660905911103 27.42558808789415 28.51903374304447 22.02371726315028 56-57 22.256734528071217 27.637285173543546 28.206969641585093 21.899010656800144 58-59 22.40407761942972 27.159212181193805 28.44323606955977 21.993474129816704 60-61 22.21450111742541 27.409261859869915 28.850661526821924 21.525575495882755 62-63 22.184960731196394 27.347368985314525 29.06127199212848 21.406398291360603 64-65 22.139365412416055 27.4098463984573 28.776305664777365 21.67448252434928 66-67 22.194523620467013 27.592984690458977 28.413179037769947 21.799312651304064 68-69 22.057523366930393 27.565809400245016 28.59887539079427 21.777791842030318 70-71 22.27879157360278 27.56241993976532 28.367906223249346 21.790882263382553 72-73 22.229286611493393 27.72182594909119 28.04353371586517 22.005353723550247 74-75 22.04584569780846 28.210183699235692 27.835616792221924 21.908353810733924 76-77 22.171906836168954 27.841267586077734 28.089062969217704 21.897762608535604 78-79 22.023370904941228 27.948213050585082 27.895417331591343 22.132998712882348 80-81 22.183691881848517 28.305904280430553 27.59566370601239 21.914740131708534 82-83 22.187689021639333 27.994918194098762 27.774413804840698 22.042978979421203 84-85 21.9831203871299 28.172645468387458 27.556718825434707 22.287515319047934 86-87 22.019128957226737 28.020638271283214 27.58989928541697 22.370333486073076 88-89 22.125250855289394 27.922038293070017 27.39292939240952 22.559781459231072 90-91 21.887225998778128 27.947511111168517 27.69189934140252 22.473363548650838 92-93 22.04044940337227 28.02677898521944 27.53196254584414 22.400809065564147 94-95 21.762641964178464 27.681093529417083 27.582253008484592 22.97401149791986 96-97 21.84884830840251 27.83059871430066 27.575531930008562 22.745021047288265 98-99 21.62878145731718 28.120657893249764 27.310255081364804 22.94030556806825 100-101 21.274124504383895 28.497308440077067 27.310961951343003 22.91760510419603 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1391.0 1 995.5 2 686.5 3 763.0 4 694.0 5 687.5 6 1125.5 7 2316.0 8 3787.5 9 4506.5 10 4061.5 11 3477.0 12 3536.5 13 3746.0 14 3918.0 15 4060.0 16 4149.0 17 4294.5 18 4498.0 19 4679.0 20 4891.0 21 5423.5 22 6010.5 23 6682.5 24 7560.5 25 8649.0 26 10045.5 27 11562.0 28 12994.0 29 15116.5 30 18004.5 31 20401.5 32 23647.0 33 27062.5 34 29994.0 35 33115.0 36 37426.5 37 41595.0 38 43813.0 39 44399.5 40 44228.5 41 45576.5 42 46651.5 43 48297.0 44 51190.0 45 53001.5 46 52691.5 47 54311.0 48 61534.0 49 60930.0 50 53652.5 51 50215.0 52 48292.0 53 48232.0 54 47645.0 55 45125.0 56 42095.5 57 38673.5 58 36269.0 59 33582.5 60 28926.5 61 23847.5 62 19260.5 63 14499.5 64 10064.0 65 7292.5 66 5210.5 67 3920.0 68 2813.0 69 1758.5 70 1177.5 71 717.5 72 501.5 73 393.5 74 279.5 75 216.5 76 149.0 77 71.0 78 35.0 79 22.5 80 12.5 81 5.5 82 5.0 83 3.5 84 3.5 85 2.0 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02796335702361386 2 0.006135147614880639 3 1.9374150362780964E-4 4 1.9374150362780964E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 1.2916100241853977E-4 10-11 0.013109841745481788 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.8435375906952417E-4 20-21 8.718367663251435E-4 22-23 0.0021311565399059064 24-25 0.011753651220087119 26-27 0.00555392310399721 28-29 2.2603175423244458E-4 30-31 0.002776961551998605 32-33 0.004746666838881337 34-35 0.0 36-37 0.00875065791385607 38-39 0.013142131996086423 40-41 0.001679093031441017 42-43 0.0 44-45 0.0 46-47 1.6145125302317471E-4 48-49 0.0037779593207422886 50-51 0.007523628390879942 52-53 0.0 54-55 0.0030352835568356845 56-57 0.003487347065300574 58-59 1.6145125302317471E-4 60-61 0.0019051247856734616 62-63 0.005715374357020385 64-65 3.229025060463494E-5 66-67 0.0026800908001847 68-69 3.8748300725561927E-4 70-71 7.426757639066037E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002195737041115176 80-81 0.002324898043533716 82-83 0.012044263475528835 84-85 0.00887981891627461 86-87 0.004488344834044256 88-89 1.6145125302317471E-4 90-91 8.395465157205085E-4 92-93 1.2916100241853977E-4 94-95 6.458050120926988E-5 96-97 0.0023894785447429855 98-99 0.0017759637832549218 100-101 2.2603175423244458E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.59535225129918 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99145296577052 33.544671100229145 2 14.422760540403404 13.440672156324693 3 5.391359142339171 7.536368350516677 4 2.597114968763737 4.8405394722667285 5 1.4330129205869497 3.3385870907705972 6 0.8814177980385602 2.4641983668102716 7 0.5760972634286331 1.8790418444326662 8 0.4230918694951593 1.5771291755030121 9 0.3276563705963511 1.3740537604787286 >10 1.735075860455653 14.876350727518005 >50 0.1387687409088982 4.465627764395375 >100 0.07703724690343368 6.488722681115008 >500 0.00348264345241339 1.140468688972243 >1k 0.0012537516428688205 0.9647573206712834 >5k 1.393057380965356E-4 0.4704676754508149 >10k+ 2.786114761930712E-4 1.5983438245448214 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14095 0.910262164544659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10529 0.6799680972324027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7248 0.4680794727647881 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2781 0.17959837386297955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2639 0.17042794269126324 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.12515701134356505 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.1037808654432967 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1585 0.10236009441669278 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.458050120926988E-5 0.0 12-13 0.0 0.0 0.0 6.458050120926988E-5 0.0 14-15 0.0 0.0 0.0 6.458050120926988E-5 0.0 16-17 0.0 0.0 0.0 6.458050120926988E-5 0.0 18-19 0.0 0.0 0.0 6.458050120926988E-5 0.0 20-21 0.0 0.0 0.0 1.2916100241853977E-4 0.0 22-23 0.0 0.0 0.0 2.5832200483707953E-4 0.0 24-25 0.0 0.0 0.0 7.749660145112387E-4 0.0 26-27 0.0 0.0 0.0 0.001130158771162223 0.0 28-29 0.0 0.0 0.0 0.0030352835568356845 0.0 30-31 0.0 0.0 0.0 0.007620499142693847 0.0 32-33 0.0 0.0 0.0 0.01411083951422547 0.0 34-35 0.0 0.0 0.0 0.01953560161580414 0.0 36-37 0.0 0.0 0.0 0.02366875369319741 0.0 38-39 0.0 0.0 0.0 0.026058232237940397 0.0 40-41 0.0 0.0 0.0 0.031063221081658814 0.0 42-43 0.0 0.0 0.0 0.03661714418565602 0.0 44-45 0.0 0.0 0.0 0.043591838316257174 0.0 46-47 0.0 0.0 0.0 0.05137378871197419 0.0 48-49 0.0 0.0 0.0 0.06077025163792296 0.0 50-51 0.0 0.0 0.0 0.07178122709410348 0.0 52-53 0.0 0.0 0.0 0.09751655682599752 0.0 54-55 0.0 0.0 0.0 0.12648091161835506 0.0 56-57 0.0 0.0 0.0 0.1644865365800104 0.0 58-59 0.0 0.0 0.0 0.2224475364153301 0.0 60-61 0.0 0.0 0.0 0.30181697240152283 0.0 62-63 0.0 0.0 0.0 0.35525733715219365 0.0 64-65 0.0 0.0 0.0 0.4053718060905871 0.0 66-67 0.0 0.0 0.0 0.5096693155435579 0.0 68-69 0.0 0.0 0.0 0.6411229257550268 0.0 70-71 0.0 0.0 0.0 0.7422559906487434 0.0 72-73 0.0 0.0 0.0 0.8360914589058126 0.0 74-75 0.0 0.0 0.0 1.02779867674553 0.0 76-77 0.0 0.0 0.0 1.163966663545276 0.0 78-79 0.0 0.0 0.0 1.2677475289885725 0.0 80-81 0.0 0.0 0.0 1.385865265700327 0.0 82-83 0.0 0.0 0.0 1.594008221097804 0.0 84-85 0.0 0.0 0.0 1.8055416528087673 0.0 86-87 0.0 0.0 0.0 2.1172394418953084 0.0 88-89 0.0 0.0 0.0 2.530812971639473 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9455 0.0 33.564762 1 GTATCAA 14655 0.0 33.03368 1 TATCAAC 18250 0.0 26.467594 2 TCAACGC 18410 0.0 26.236721 4 ATCAACG 18430 0.0 26.208248 3 CAACGCA 18745 0.0 25.798168 5 AACGCAG 19270 0.0 25.119963 6 GTACATG 10610 0.0 24.89591 1 GAGTACT 11045 0.0 23.952227 12-13 CGTATAG 160 1.8574592E-6 23.7541 1 TACATGG 10975 0.0 23.585678 2 ACATGGG 10810 0.0 22.714712 3 ACGCAGA 21320 0.0 22.526333 7 GTACTTT 11730 0.0 22.432734 14-15 AGTACTT 11335 0.0 22.312822 12-13 CGCAGAG 21465 0.0 22.241377 8 GGACCGT 195 4.22031E-7 21.92474 6 TAAGGTG 615 0.0 21.627657 5 GCAGAGT 22090 0.0 21.289528 9 CATGGGA 6950 0.0 20.911203 4 >>END_MODULE