FastQCFastQC Report
Thu 26 May 2016
SRR937088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559658
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136280.8737813033370136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.6066073459694369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63750.4087434552959688No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22340.14323653005979517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.1399024657969888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141700.033.877721
GGTATCA90300.031.7389221
TCAACGC175450.027.1179754
TATCAAC176150.027.064182
ATCAACG176600.026.9952163
GTACATG111100.026.69521
CAACGCA178500.026.6546175
AACGCAG181050.026.3537276
TACATGG114200.025.5551742
ATACCGT1351.3380257E-524.641736
ACATGGG114400.024.2225073
GTATAGG4750.024.0148721
ACGCAGA201150.023.6928927
CGCAGAG201850.023.5165678
GAGTACT95600.023.43704412-13
AGTACTT99500.023.2109112-13
CATGGGA76300.022.8622044
GCAGAGT207600.022.2930159
ATGGGAG27650.022.0034835
GTACTTT102050.021.76980814-15
Produced by FastQC (version 0.11.5)