##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1559658 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83288131115924 33.0 31.0 34.0 30.0 34.0 2 31.90065642596005 33.0 31.0 34.0 30.0 34.0 3 31.905803067082655 33.0 31.0 34.0 30.0 34.0 4 35.52473170400177 37.0 35.0 37.0 33.0 37.0 5 35.535262217742606 37.0 35.0 37.0 33.0 37.0 6 35.616939739353114 37.0 35.0 37.0 33.0 37.0 7 35.56523802013005 37.0 35.0 37.0 33.0 37.0 8 35.53718058702613 37.0 35.0 37.0 33.0 37.0 9 37.15729922842059 39.0 37.0 39.0 33.0 39.0 10-11 37.02908394019715 39.0 37.0 39.0 33.0 39.0 12-13 36.94330103137996 39.0 37.0 39.0 33.0 39.0 14-15 38.05441898159725 40.0 38.0 41.0 33.0 41.0 16-17 38.00480618186808 40.0 38.0 41.0 33.0 41.0 18-19 38.021395716240356 40.0 38.0 41.0 33.0 41.0 20-21 37.978279853660226 40.0 38.0 41.0 33.0 41.0 22-23 37.86585777138322 40.0 38.0 41.0 33.0 41.0 24-25 37.813574834995876 40.0 37.5 41.0 32.5 41.0 26-27 37.82160159470858 40.0 37.0 41.0 33.0 41.0 28-29 37.74340817025271 40.0 37.0 41.0 33.0 41.0 30-31 37.54137701983383 40.0 37.0 41.0 32.0 41.0 32-33 37.29738827358305 40.0 36.0 41.0 32.0 41.0 34-35 37.1055346749095 39.5 36.0 41.0 31.0 41.0 36-37 36.909123666855166 39.0 35.5 41.0 31.0 41.0 38-39 36.72616464635196 39.0 35.0 41.0 30.5 41.0 40-41 36.50931838903144 39.0 35.0 41.0 30.0 41.0 42-43 36.234728382760835 39.0 35.0 41.0 30.0 41.0 44-45 35.89152076929686 39.0 35.0 40.5 29.5 41.0 46-47 35.405435037681336 39.0 35.0 40.0 26.5 41.0 48-49 35.04749214250816 38.5 35.0 40.0 25.0 41.0 50-51 34.30072554367688 38.0 34.0 39.5 23.5 40.5 52-53 34.459888642253624 38.0 34.5 40.0 24.0 40.5 54-55 34.826202282808154 39.0 35.0 40.0 24.5 41.0 56-57 34.693837366909925 38.5 35.0 40.0 24.0 41.0 58-59 34.466841127990875 38.0 34.0 40.0 23.0 41.0 60-61 34.175417303024126 38.0 34.0 40.0 23.0 41.0 62-63 33.86472451011696 37.0 34.0 40.0 22.0 41.0 64-65 33.51895383475095 37.0 33.5 40.0 21.5 41.0 66-67 33.131775684156395 36.0 33.0 39.0 20.0 41.0 68-69 32.74004942109104 36.0 33.0 39.0 18.5 41.0 70-71 32.32734195573645 35.0 32.0 39.0 17.5 40.0 72-73 31.94535853372983 35.0 32.0 37.5 14.0 40.0 74-75 31.557663923757644 35.0 32.0 37.0 11.0 39.0 76-77 31.12100601542133 35.0 31.0 36.5 7.5 39.0 78-79 30.678970325545727 35.0 31.0 36.0 6.0 39.0 80-81 30.266282095177274 35.0 31.0 36.0 2.0 37.5 82-83 29.886132087932097 34.0 30.0 35.0 2.0 37.0 84-85 29.535631208893232 34.0 30.0 35.0 2.0 36.5 86-87 29.206063124095152 34.0 29.5 35.0 2.0 36.0 88-89 28.863114862360852 34.0 29.0 35.0 2.0 36.0 90-91 28.50222741139404 34.0 29.0 35.0 2.0 35.5 92-93 28.14476667320656 34.0 29.0 35.0 2.0 35.0 94-95 27.80081113936517 34.0 28.5 35.0 2.0 35.0 96-97 27.33945679116832 34.0 27.0 35.0 2.0 35.0 98-99 26.80838683865309 34.0 25.5 35.0 2.0 35.0 100-101 24.672674714584865 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14194571735162853 1101 2 0.2696636247965323 1101 3 0.2511062458598907 1101 4 0.3054279185451847 1101 5 0.23638958299929413 1101 6 0.09413405944384579 1101 7 0.20130546119696646 1101 8 0.2472873579423407 1101 9 0.23291923483857602 1101 10-11 0.3582489234426376 1101 12-13 0.4446702384560197 1101 14-15 0.48305588384003073 1101 16-17 0.5235437915999341 1101 18-19 0.5323235110694213 1101 20-21 0.5747425513898179 1101 22-23 0.5540852395723093 1101 24-25 0.5297212209777769 1101 26-27 0.5316263771073224 1101 28-29 0.5565253564778629 1101 30-31 0.4798066302207644 1101 32-33 0.4813704678927806 1101 34-35 0.5603928567904504 1101 36-37 0.533568472794677 1101 38-39 0.49109616520721744 1101 40-41 0.3969984327803644 1101 42-43 0.4224688670672876 1101 44-45 0.5496433792225446 1101 46-47 0.5746995226399108 1101 48-49 0.4972623494699704 1101 50-51 0.5971527638869958 1101 52-53 0.6680858649744295 1101 54-55 0.8017140258404183 1101 56-57 0.901362879338734 1101 58-59 0.820864503003925 1101 60-61 0.8914211728338017 1101 62-63 0.8367628600443666 1101 64-65 0.6090800300655417 1101 66-67 0.5771155544029725 1101 68-69 0.5828480005198031 1101 70-71 0.5496110210725078 1101 72-73 0.5170529813598215 1101 74-75 0.5603866307545786 1101 76-77 0.6649320984011275 1101 78-79 0.6544059066027863 1101 80-81 0.6368799779478813 1101 82-83 0.5918230020746158 1101 84-85 0.5431510099721955 1101 86-87 0.6381970258707561 1101 88-89 0.6788181396282305 1101 90-91 0.7912108516342151 1101 92-93 0.7337716653551922 1101 94-95 0.8088207623819983 1101 96-97 0.8709517652221059 1101 98-99 0.7944809922100227 1101 100-101 0.9751923173182888 1102 1 0.2977921352035615 1102 2 0.3224372428078546 1102 3 0.24891299560762903 1102 4 0.36665928796886504 1102 5 0.19010027320631195 1102 6 0.18385422320653078 1102 7 0.18772177062360385 1102 8 0.06953575277238144 1102 9 0.1633782588181134 1102 10-11 0.20285176036765762 1102 12-13 0.17386617523899872 1102 14-15 0.014694442625561521 1102 16-17 0.2422510111691949 1102 18-19 0.23725470888935263 1102 20-21 0.09950426639903753 1102 22-23 0.2146424054752245 1102 24-25 0.09035952910613787 1102 26-27 0.14143848063515918 1102 28-29 0.06450943010679566 1102 30-31 0.09886669796247816 1102 32-33 0.1506872985577914 1102 34-35 0.20475049958469782 1102 36-37 0.27900394142717744 1102 38-39 0.36308313746139476 1102 40-41 0.3229676405108819 1102 42-43 0.19971658705109263 1102 44-45 0.22965812943303376 1102 46-47 0.492090952735488 1102 48-49 0.7020502228045444 1102 50-51 0.720715020058627 1102 52-53 0.7689082133107661 1102 54-55 0.5709394309088438 1102 56-57 0.6032619287696122 1102 58-59 0.525157007069069 1102 60-61 0.44222922085715055 1102 62-63 0.3508276195741544 1102 64-65 0.5177080998125092 1102 66-67 0.22254604202757378 1102 68-69 0.1911764064044661 1102 70-71 0.2107424489828631 1102 72-73 0.31692363583212924 1102 74-75 0.4744396608141557 1102 76-77 0.44826516393833415 1102 78-79 0.4959669544783054 1102 80-81 0.46857300646495403 1102 82-83 0.5435022444472501 1102 84-85 0.38292082313619247 1102 86-87 0.34300289510483495 1102 88-89 0.5135736846147658 1102 90-91 0.3035284021352105 1102 92-93 0.4417473837450707 1102 94-95 0.6084335291872769 1102 96-97 0.4027599996315523 1102 98-99 0.34308598864297934 1102 100-101 0.380213655313419 1103 1 0.20240488201380558 1103 2 0.31922258380342683 1103 3 0.26429069977931263 1103 4 0.17145325362692887 1103 5 0.2241035429813394 1103 6 0.1797573757105866 1103 7 0.183777986317196 1103 8 0.12639059308862244 1103 9 0.16913057933575004 1103 10-11 0.18177021122208004 1103 12-13 0.19428516994918255 1103 14-15 0.31650301203922737 1103 16-17 0.4074468656469392 1103 18-19 0.33022382930392524 1103 20-21 0.2890250491263444 1103 22-23 0.30618182937326566 1103 24-25 0.3287654075294739 1103 26-27 0.36578180302939955 1103 28-29 0.4541123556390687 1103 30-31 0.41625185408693 1103 32-33 0.3023498436964971 1103 34-35 0.39786810125385585 1103 36-37 0.3037500424661488 1103 38-39 0.473653362587136 1103 40-41 0.5172222593005387 1103 42-43 0.6021635395331089 1103 44-45 0.518701693673755 1103 46-47 0.5002318558680656 1103 48-49 0.4908220251783817 1103 50-51 0.5127169205655662 1103 52-53 0.5480559601276553 1103 54-55 0.5431712449433732 1103 56-57 0.6851070739771075 1103 58-59 0.6463403880589524 1103 60-61 0.684289358421438 1103 62-63 0.615638558313016 1103 64-65 0.7157888003040398 1103 66-67 0.588054132130992 1103 68-69 0.5458920475366966 1103 70-71 0.649315302412738 1103 72-73 0.5777119344999058 1103 74-75 0.6476967325991296 1103 76-77 0.5972290157917612 1103 78-79 0.7219953000544805 1103 80-81 0.6112886595500591 1103 82-83 0.6453349188230781 1103 84-85 0.528870890669932 1103 86-87 0.4970764344968579 1103 88-89 0.38759139080515936 1103 90-91 0.6526393075388377 1103 92-93 0.6365496765718852 1103 94-95 0.693620664707634 1103 96-97 0.7998356294483919 1103 98-99 0.6675693256728508 1103 100-101 0.8366153821052542 1104 1 0.19734870957345052 1104 2 0.15955631317381958 1104 3 0.28126955772203743 1104 4 0.2637167188298406 1104 5 0.14799433570370013 1104 6 0.14711214818626672 1104 7 0.15866513636897395 1104 8 0.1976772645975231 1104 9 0.32155823614547074 1104 10-11 0.35872130075921405 1104 12-13 0.13987496854750248 1104 14-15 0.1937863810250562 1104 16-17 0.4888678713703527 1104 18-19 0.45113097865479546 1104 20-21 0.39109352391179186 1104 22-23 0.34142815457764186 1104 24-25 0.2577948326939321 1104 26-27 0.2594688714457689 1104 28-29 0.2941298165444408 1104 30-31 0.4313248552393105 1104 32-33 0.24166649766203108 1104 34-35 0.3139593803527845 1104 36-37 0.33257084382618984 1104 38-39 0.47388906075879333 1104 40-41 0.39693957267122215 1104 42-43 0.45932460659302166 1104 44-45 0.3559400807286366 1104 46-47 0.51556855723436 1104 48-49 0.5574682600890597 1104 50-51 0.4419978487353404 1104 52-53 0.6201419782784754 1104 54-55 0.7782868710449904 1104 56-57 0.8273983350722744 1104 58-59 0.698899706004795 1104 60-61 0.7484042430702047 1104 62-63 0.7082775601072129 1104 64-65 0.6708417430609899 1104 66-67 0.3219125867893311 1104 68-69 0.5202406307020766 1104 70-71 0.6381308057176653 1104 72-73 0.6547769906311238 1104 74-75 0.7142630843831199 1104 76-77 0.702860621610963 1104 78-79 0.8509424713671976 1104 80-81 0.6615683020309753 1104 82-83 0.7590981115643984 1104 84-85 0.9226923001724856 1104 86-87 1.0214789258883776 1104 88-89 0.7987733793418457 1104 90-91 0.6295367637165015 1104 92-93 0.6708684985483018 1104 94-95 1.013069165344291 1104 96-97 0.8599058510115434 1104 98-99 0.6699793910155307 1104 100-101 0.6655661954013787 1105 1 0.15698497446259196 1105 2 0.1730390857637012 1105 3 0.20963546527170607 1105 4 0.2073817002735936 1105 5 0.20461649012788996 1105 6 0.2065247883968624 1105 7 0.14621367865362345 1105 8 0.11713899242833747 1105 9 0.16339905158165635 1105 10-11 0.2706870759444442 1105 12-13 0.15515734849577711 1105 14-15 0.3248998894692221 1105 16-17 0.3433608428481847 1105 18-19 0.22515690546556755 1105 20-21 0.2513466486474698 1105 22-23 0.2523310818795963 1105 24-25 0.07935621373583501 1105 26-27 0.257084605007428 1105 28-29 0.2593971348438231 1105 30-31 0.24512837417469768 1105 32-33 0.17355037605887702 1105 34-35 -0.04264036708636354 1105 36-37 0.030279469921865143 1105 38-39 0.4488945141144214 1105 40-41 0.348362416221498 1105 42-43 0.4773021313482815 1105 44-45 0.33571592184382126 1105 46-47 0.22036394881497046 1105 48-49 0.1498295556579663 1105 50-51 0.15106392263091095 1105 52-53 0.18927049495090387 1105 54-55 -0.06434115668032092 1105 56-57 0.22342510177400499 1105 58-59 0.14007395073358708 1105 60-61 0.2923345469593741 1105 62-63 0.25660987201838026 1105 64-65 0.3724541556403622 1105 66-67 0.2831544382888964 1105 68-69 0.1628147184335802 1105 70-71 0.19012391445684074 1105 72-73 0.20666140323423932 1105 74-75 0.3419736247179159 1105 76-77 0.2852237275455529 1105 78-79 0.4217507699647598 1105 80-81 0.2647687911264889 1105 82-83 0.4261518304561598 1105 84-85 0.46456145821889194 1105 86-87 0.541727940203625 1105 88-89 0.440016774103956 1105 90-91 0.4900696047886548 1105 92-93 0.4952923348318876 1105 94-95 0.5859924013877844 1105 96-97 0.48344846954227094 1105 98-99 0.4036553088486734 1105 100-101 0.44705988063814317 1106 1 0.36619937442739214 1106 2 0.2252349247634804 1106 3 0.3393654394498107 1106 4 0.2105778627600543 1106 5 0.1329918943843893 1106 6 0.20390348700260574 1106 7 0.2292564579338574 1106 8 0.3082116563370789 1106 9 0.33315826178525754 1106 10-11 0.28259150803790334 1106 12-13 0.40026838102126305 1106 14-15 0.4291070089466231 1106 16-17 0.33659878970999557 1106 18-19 0.39694259268735976 1106 20-21 0.3603247892590673 1106 22-23 0.15381553537044113 1106 24-25 0.2406854180645297 1106 26-27 0.23415432901706623 1106 28-29 0.2792577503811202 1106 30-31 0.47246800993032423 1106 32-33 0.5359277750141089 1106 34-35 0.5120095298786467 1106 36-37 0.33596969842405855 1106 38-39 0.3350701844937163 1106 40-41 0.4912172309988847 1106 42-43 0.47976046716005527 1106 44-45 0.4045876214852129 1106 46-47 0.5998698453171656 1106 48-49 0.6022749764473261 1106 50-51 0.5891092971380374 1106 52-53 0.6841751723075689 1106 54-55 0.7110839186708873 1106 56-57 0.6165439207950243 1106 58-59 0.605667027303781 1106 60-61 0.6937707218964064 1106 62-63 0.7294549170091003 1106 64-65 0.5503891454101932 1106 66-67 0.42763259397479914 1106 68-69 0.5572317620260634 1106 70-71 0.6690938171582346 1106 72-73 0.6739609331600072 1106 74-75 0.8771285601713217 1106 76-77 0.801489526040239 1106 78-79 0.9374015770925439 1106 80-81 0.8826018703693173 1106 82-83 0.7728264770041804 1106 84-85 0.8115477764434154 1106 86-87 0.8753046354734657 1106 88-89 0.938306450315789 1106 90-91 0.9593815559511292 1106 92-93 0.907880244578287 1106 94-95 0.954578992742622 1106 96-97 1.006767825860809 1106 98-99 1.0254515073505281 1106 100-101 1.3264863314188702 1107 1 0.19379040489564758 1107 2 0.23631745830984485 1107 3 0.2807376839555822 1107 4 0.18482319531874225 1107 5 0.1715733944458364 1107 6 0.18491954233036267 1107 7 0.18965420996450888 1107 8 0.18346276523845972 1107 9 0.2672757773992629 1107 10-11 0.30846818256278397 1107 12-13 0.2831565523674726 1107 14-15 0.17673915535512918 1107 16-17 0.2358636188218739 1107 18-19 0.35761296127835607 1107 20-21 0.4112475516245695 1107 22-23 0.3757942399948604 1107 24-25 0.356512150129376 1107 26-27 0.3801704402088717 1107 28-29 0.3461323802817944 1107 30-31 0.5252190908996752 1107 32-33 0.3197318933118183 1107 34-35 0.24325496299262284 1107 36-37 0.2572315555685094 1107 38-39 0.3441183728225994 1107 40-41 0.3598182834775798 1107 42-43 0.46420791584892385 1107 44-45 0.516900057939857 1107 46-47 0.5251103400491743 1107 48-49 0.7376257468125615 1107 50-51 0.6236272462570582 1107 52-53 0.5959942174792374 1107 54-55 0.7884411022289797 1107 56-57 0.9440907069362723 1107 58-59 0.7600240879609643 1107 60-61 0.7812451082298324 1107 62-63 0.6457707946891844 1107 64-65 0.6675916972799669 1107 66-67 0.7268315035085564 1107 68-69 0.7894116889558802 1107 70-71 0.724964793550086 1107 72-73 0.6742650570441278 1107 74-75 0.7253211347218951 1107 76-77 0.8494966319473001 1107 78-79 0.6365562422992994 1107 80-81 0.6649845837979207 1107 82-83 0.8008487779326394 1107 84-85 0.8081465496688942 1107 86-87 0.76951868416457 1107 88-89 0.7648832131058612 1107 90-91 1.0205868786777401 1107 92-93 1.0084362035964212 1107 94-95 1.0196447447285877 1107 96-97 1.165631327642874 1107 98-99 1.064862700271437 1107 100-101 1.099517403003066 1108 1 0.0714117563422505 1108 2 0.027853348434049963 1108 3 0.18695621801942153 1108 4 0.18473049295953103 1108 5 0.11124466419047963 1108 6 0.12408970669549291 1108 7 0.11752921129951233 1108 8 0.09628623559613203 1108 9 -0.013432092689278363 1108 10-11 -0.06257557337796271 1108 12-13 0.16527567703505497 1108 14-15 0.39995618353963636 1108 16-17 0.5555489861603107 1108 18-19 0.4434782010976477 1108 20-21 0.4327769269516466 1108 22-23 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#Quality Count 2 2638.0 3 904.0 4 896.0 5 1046.0 6 1188.0 7 1475.0 8 1794.0 9 2273.0 10 2878.0 11 4002.0 12 5521.0 13 7668.0 14 8748.0 15 11394.0 16 15333.0 17 21000.0 18 18025.0 19 9703.0 20 9013.0 21 9299.0 22 10111.0 23 10760.0 24 12107.0 25 14007.0 26 16176.0 27 19045.0 28 22506.0 29 27409.0 30 33693.0 31 41964.0 32 53561.0 33 71034.0 34 98003.0 35 142842.0 36 216660.0 37 314047.0 38 275243.0 39 45683.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.767136080374165 18.901512916359867 14.98805727377449 21.343293729491478 2 15.189198791970155 20.833512374353287 40.66197931931918 23.31530951435738 3 18.795328455544205 26.878760069338227 30.213818521361652 24.11209295375592 4 12.373392997117305 16.97993146704401 37.97119614511744 32.67547939072124 5 13.053275111412319 38.10609327829462 33.4946758260941 15.345955784198962 6 29.866283592781162 36.70146939131819 18.12058868083424 15.311658335066408 7 27.25314330547624 34.28404999008846 21.116520581040795 17.34628612339451 8 26.61311902297475 33.235966202996345 20.170075906576322 19.980838867452576 9 27.496156698893078 16.32228806664631 20.66970965432105 35.511845580139564 10-11 24.37541605511577 27.155105347160397 27.061524024927213 21.407954572796612 12-13 26.438308922954523 24.927041545496976 27.720106442190627 20.914543089357863 14-15 23.385493637760206 25.14723114977752 27.401722666203028 24.06555254625924 16-17 21.084972653408414 28.869321396542897 27.620474469458163 22.425231480590526 18-19 21.12526014868572 26.57163808345801 31.354717063444205 20.94838470441207 20-21 22.54775259905202 25.29815603710443 31.303649402308654 20.850441961534898 22-23 21.974268195695117 25.57582287898612 31.321897688498936 21.128011236819827 24-25 21.697356448829808 25.672525906827115 31.489114355533808 21.141003288809262 26-27 21.388973845535354 25.8181645645507 31.483458460046972 21.309403129866975 28-29 21.148402088528417 25.934377939517827 31.63605667470522 21.281163297248533 30-31 21.283787358011423 25.787731827978984 31.520429908273883 21.408050905735713 32-33 21.07851278088942 26.053874342360483 31.26337320581833 21.604239670931772 34-35 21.52101443281518 25.829667261060894 31.262186844972113 21.387131461151817 36-37 21.7116159359004 25.577642085365675 31.11505521062496 21.595686768108962 38-39 21.439563173404665 25.90615486415852 31.169996127241905 21.484285835194907 40-41 21.410012997313974 25.920472083988166 31.257129446706845 21.412385471991012 42-43 21.361351682517984 26.088021456825068 30.598449471241608 21.952177389415343 44-45 21.35947759354231 26.248750904462188 30.360563429047367 22.031208072948143 46-47 21.871300222006717 26.392414904104317 29.890631637159277 21.84565323672969 48-49 21.831027907471785 26.749618825713906 29.22455878034487 22.194794486469434 50-51 21.787214643491907 26.903723195531242 29.124861826025178 22.18420033495167 52-53 21.986410417696593 27.243637217014616 28.738754162186286 22.03119820310251 54-55 21.666366352309137 27.103509702291216 28.753485944326208 22.476638001073436 56-57 21.88585555434081 27.306839862869953 28.44101936370401 22.366285219085224 58-59 22.08369414291041 26.73990900705739 28.79201113895708 22.384385711075126 60-61 21.91756536112722 26.98933428484503 29.054672489866945 22.038427864160806 62-63 21.92021585949127 26.996607317703443 29.240484170581833 21.84269265222345 64-65 21.796900972898182 27.127275035529525 29.037036942047045 22.03878704952525 66-67 21.790363370457147 27.318501550183523 28.60847741484091 22.282657664518418 68-69 21.68551394390622 27.23321713545119 28.96874459782479 22.112524322817794 70-71 21.881360156507363 27.187252309744213 28.698373371679647 22.233014162068777 72-73 21.76761179057599 27.33191780079719 28.3938877976201 22.506582611006724 74-75 21.533520962745367 27.786242262531125 28.30619888845925 22.374037886264258 76-77 21.66101260140443 27.456311924840477 28.488055920736205 22.394619553018885 78-79 21.588172558133866 27.48233329421294 28.42519892079018 22.504295226863015 80-81 21.68114578778364 27.79262994074142 28.0950023692524 22.431221902222543 82-83 21.74813713603324 27.443785392170668 28.344937919499134 22.46313955229696 84-85 21.558646171051013 27.581167665285015 28.11602269462798 22.744163469035993 86-87 21.695780991098147 27.470265420148355 28.148446977701912 22.68550661105159 88-89 21.761856453418837 27.337541562976387 27.927080730719382 22.973521252885394 90-91 21.510575859138214 27.25717218043907 28.334771243926298 22.897480716496414 92-93 21.70706651823614 27.29854837638877 28.238586997832233 22.755798107542855 94-95 21.385255346858894 27.09085860746011 28.294923842569286 23.228962203111706 96-97 21.33550211828471 27.0739968006895 28.356318157289493 23.2341829237363 98-99 21.194010321458396 27.33829907599472 28.295208334135086 23.172482268411795 100-101 21.088602331853068 27.37328705815773 28.404885970703937 23.133224639285263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 856.0 1 629.5 2 515.0 3 656.5 4 591.5 5 608.5 6 1067.5 7 2247.5 8 3886.5 9 4661.0 10 4001.5 11 3316.0 12 3279.0 13 3404.0 14 3663.5 15 3823.5 16 3887.5 17 3979.5 18 3987.5 19 4193.0 20 4542.0 21 4968.0 22 5619.5 23 6532.0 24 7405.0 25 8386.0 26 9928.5 27 11542.0 28 12885.5 29 14887.5 30 17822.5 31 20708.5 32 24283.5 33 27802.5 34 30978.0 35 34132.5 36 38612.0 37 42720.5 38 44252.5 39 45001.0 40 45315.5 41 46350.5 42 47224.0 43 49247.5 44 52428.0 45 54634.5 46 54162.5 47 54536.0 48 61940.0 49 62146.0 50 54243.5 51 50472.5 52 48793.0 53 48527.0 54 47779.5 55 45216.0 56 42458.0 57 38983.0 58 36109.0 59 33271.5 60 28609.0 61 23768.0 62 19184.5 63 14295.0 64 10053.0 65 7349.0 66 5205.0 67 3802.5 68 2681.5 69 1651.5 70 1045.0 71 707.5 72 497.5 73 349.0 74 242.5 75 189.0 76 136.0 77 69.0 78 37.0 79 27.0 80 15.5 81 8.5 82 9.0 83 9.0 84 8.5 85 11.0 86 7.0 87 3.5 88 4.5 89 1.5 90 1.5 91 1.5 92 0.5 93 1.5 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035264141241220834 2 0.02724956368639791 3 0.02212023405131125 4 0.04488163430700833 5 0.09502083148998049 6 0.1111782198405035 7 0.05571734316112891 8 0.08303102346796541 9 0.07065651572331882 10-11 0.07184267320143262 12-13 0.06831625907731054 14-15 0.06216106351520653 16-17 0.050010963942095 18-19 0.05988492348963684 20-21 0.05568528485090962 22-23 0.0319300769784145 24-25 0.008142810795700084 26-27 0.00250054819710475 28-29 0.005834612459911083 30-31 0.0044561051204815415 32-33 0.006604011905174083 34-35 0.00557814597815675 36-37 0.005449912737279583 38-39 0.002949364540174833 40-41 0.006732245146051249 42-43 0.003911113846753583 44-45 0.0018914403029382082 46-47 0.0013143907189909583 48-49 2.564664817543333E-4 50-51 0.0019234986131574996 52-53 0.005193446255525249 54-55 0.011605108299383583 56-57 0.0074695862810949575 58-59 0.005546087667937459 60-61 0.002212023405131125 62-63 0.008014577554822917 64-65 0.002532606507324042 66-67 0.020934076573197458 68-69 0.04356724358801737 70-71 0.05651880091661121 72-73 0.0036225890547799578 74-75 0.012887440708155248 76-77 0.019747919095083666 78-79 0.02365903294183725 80-81 0.07370205519415154 82-83 0.027730438339687292 84-85 0.010899825474559166 86-87 0.03132096908424796 88-89 0.04670895798950796 90-91 0.043791651759552415 92-93 0.04356724358801737 94-95 0.012246274503769417 96-97 0.016638263003812374 98-99 0.036931173372624 100-101 0.07405469660656375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1559658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.6742586282548 #Duplication Level Percentage of deduplicated Percentage of total 1 73.30656548404643 35.68142727519572 2 14.001410809562852 13.630165818102094 3 5.089471719101094 7.431787882101457 4 2.3821165066763186 4.63791019714392 5 1.35927232222794 3.308078627917562 6 0.8241618954984964 2.406928155182791 7 0.5571941759764654 1.8984709399345052 8 0.3797245810397639 1.4786249972028134 9 0.29621316914606366 1.2976160763699438 >10 1.6023917672453356 14.320721421269864 >50 0.1302655186926948 4.404578832878202 >100 0.06672840829418669 5.829974418023234 >500 0.003033052274279669 1.0081310658593376 >1k 0.0010549747040972763 0.7743596828299407 >5k 2.637436760243191E-4 1.016475458993281 >10k+ 1.3187183801215954E-4 0.8747491509952281 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13628 0.8737813033370136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9461 0.6066073459694369 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6375 0.4087434552959688 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2234 0.14323653005979517 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2182 0.1399024657969888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.411662043858333E-5 0.0 4 0.0 0.0 0.0 6.411662043858333E-5 0.0 5 0.0 0.0 0.0 1.2823324087716666E-4 0.0 6 0.0 0.0 0.0 1.2823324087716666E-4 0.0 7 0.0 0.0 0.0 1.9234986131575E-4 0.0 8 0.0 0.0 0.0 1.9234986131575E-4 0.0 9 0.0 0.0 0.0 1.9234986131575E-4 0.0 10-11 0.0 0.0 0.0 2.564664817543333E-4 0.0 12-13 0.0 0.0 0.0 2.564664817543333E-4 0.0 14-15 0.0 0.0 0.0 2.88524791973625E-4 0.0 16-17 0.0 0.0 0.0 4.167580328507917E-4 0.0 18-19 0.0 0.0 0.0 5.449912737279584E-4 0.0 20-21 0.0 0.0 0.0 6.411662043858333E-4 0.0 22-23 0.0 0.0 0.0 8.976326861401666E-4 0.0 24-25 0.0 0.0 0.0 0.0012823324087716664 0.0 26-27 0.0 0.0 0.0 0.0024043732664468746 0.0 28-29 0.0 0.0 0.0 0.004904921463551625 0.0 30-31 0.0 0.0 0.0 0.009521318135129624 0.0 32-33 0.0 0.0 0.0 0.015644455387014332 0.0 34-35 0.0 0.0 0.0 0.02061349347100454 0.0 36-37 0.0 0.0 0.0 0.024107849284907334 0.0 38-39 0.0 0.0 0.0 0.027089272135301455 0.0 40-41 0.0 0.0 0.0 0.032635359803238916 0.0 42-43 0.0 0.0 0.0 0.03818144747117637 0.0 44-45 0.0 0.0 0.0 0.04404811824130675 0.0 46-47 0.0 0.0 0.0 0.05260768706985762 0.0 48-49 0.0 0.0 0.0 0.062096946894767954 0.0 50-51 0.0 0.0 0.0 0.07527291239489683 0.0 52-53 0.0 0.0 0.0 0.10136837691340024 0.0 54-55 0.0 0.0 0.0 0.12990027300856982 0.0 56-57 0.0 0.0 0.0 0.16711997117316746 0.0 58-59 0.0 0.0 0.0 0.22415170505328733 0.0 60-61 0.0 0.0 0.0 0.3047142386343673 0.0 62-63 0.0 0.0 0.0 0.35783485866773357 0.0 64-65 0.0 0.0 0.0 0.40611467385798683 0.0 66-67 0.0 0.0 0.0 0.5119712142020879 0.0 68-69 0.0 0.0 0.0 0.6488601988384632 0.0 70-71 0.0 0.0 0.0 0.7490744765839691 0.0 72-73 0.0 0.0 0.0 0.8430053255264937 0.0 74-75 0.0 0.0 0.0 1.0409653911306196 0.0 76-77 0.0 0.0 0.0 1.1725326962705926 0.0 78-79 0.0 0.0 0.0 1.2703105424394323 0.0 80-81 0.0 0.0 0.0 1.3791485056339274 0.0 82-83 0.0 0.0 0.0 1.579480886194281 0.0 84-85 0.0 0.0 0.0 1.7657396685683655 0.0 86-87 0.0 0.0 0.0 2.0706462570640487 0.0 88-89 0.0 0.0 0.0 2.492758027721462 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14170 0.0 33.87772 1 GGTATCA 9030 0.0 31.738922 1 TCAACGC 17545 0.0 27.117975 4 TATCAAC 17615 0.0 27.06418 2 ATCAACG 17660 0.0 26.995216 3 GTACATG 11110 0.0 26.6952 1 CAACGCA 17850 0.0 26.654617 5 AACGCAG 18105 0.0 26.353727 6 TACATGG 11420 0.0 25.555174 2 ATACCGT 135 1.3380257E-5 24.64173 6 ACATGGG 11440 0.0 24.222507 3 GTATAGG 475 0.0 24.014872 1 ACGCAGA 20115 0.0 23.692892 7 CGCAGAG 20185 0.0 23.516567 8 GAGTACT 9560 0.0 23.437044 12-13 AGTACTT 9950 0.0 23.21091 12-13 CATGGGA 7630 0.0 22.862204 4 GCAGAGT 20760 0.0 22.293015 9 ATGGGAG 2765 0.0 22.003483 5 GTACTTT 10205 0.0 21.769808 14-15 >>END_MODULE