FastQCFastQC Report
Thu 26 May 2016
SRR937088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559658
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141650.908211928512533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108710.6970117807878394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.47875880481490174No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28620.1835017676952255No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.1697166943009301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.12643797550488634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16280.10438185807401368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.10374069186962784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99900.032.242071
GTATCAA151300.032.1214681
GTACATG105900.026.5124721
TATCAAC185700.026.142192
TCAACGC187000.026.0104224
ATCAACG187100.025.9719683
CAACGCA189350.025.687615
TACATGG108450.025.6667612
AACGCAG191900.025.3982166
ACATGGG109950.024.409353
GAGTACT110200.024.11767412-13
ACGCAGA211350.022.9260237
CGCAGAG212350.022.7516788
GTACTTT117000.022.53217714-15
AGTACTT114350.022.2869412-13
GCAGAGT219950.021.6199599
TATAGGG3750.021.5337982
CATGGGA73650.021.4763534
CATGGGG37200.021.0683294
AGAGTAC177750.020.8196110-11
Produced by FastQC (version 0.11.5)