FastQCFastQC Report
Thu 26 May 2016
SRR937087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568687
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99991.7582606952506388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72551.275745708975236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.873942959835551No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.28152568987861515No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15460.27185428891464025No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15190.267106510259598No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14860.26130366968121305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.225959095249232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.2219146912097516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11940.20995732274520076No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8420.14806035657576136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.13663051907288193No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7010.12326640137720749No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.12133212118441251No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.1079680034887381No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5890.10357191214147676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.037.0707131
GTATCAA79950.035.2484441
CGTCACT702.046205E-433.944991
TATCAAC100350.028.0379542
ATCAACG100750.027.9290963
TCAACGC101150.027.8186514
CAACGCA101750.027.6546085
AACGCAG103150.027.2331876
TACGCTG700.00836109827.1607765
GGGATAG1457.8305493E-726.2241977
GAGTACT60300.025.3681812-13
CCTATAC2251.0186341E-1025.3500583
GTACATG48200.025.2404231
ACGCAGA112850.024.555417
CGCAGAG112850.024.4290528
TACATGG49600.023.9552292
CCCTATA1801.9366962E-723.7635842
GTCTATA1401.770782E-523.7614921
GTACTTT65500.023.49927514-15
AGTACTT62150.023.46648212-13
Produced by FastQC (version 0.11.5)