FastQCFastQC Report
Thu 26 May 2016
SRR937087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568687
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78331.3773833409239176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60241.0592821710360885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.7230691047975424No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30670.5393124864820191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21650.3807015106728306No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.2642930117973507No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.23211362313539785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.19958254716566404No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9950.17496443562100067No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8270.14542270176740457No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8210.14436763984406184No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6890.12115627753052206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.10286853752591496No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG550.00257887434.5445253
CGTATAG1255.63341E-934.2050971
GTATCAA85650.029.3417951
GTAAGGT2002.3646862E-1128.496734
GGTATCA59550.027.6825471
GTACATG51700.025.0859431
GTATAGT1201.7076086E-423.753541
GACCGGG500.001654739723.74727490-91
AGTACTT59350.023.7283112-13
TACATGG53850.023.6411842
ATCAACG107650.023.2529753
TATCAAC107950.023.2343982
TCAACGC108850.022.9946064
ACATGGG52650.022.8246483
CAACGCA110350.022.6820355
AGAGTAC89200.022.3648710-11
AACGCAG112300.022.3324386
GAGTACT58950.022.27789512-13
ATGGGAT14400.021.438515
CATGGGG11200.021.2029254
Produced by FastQC (version 0.11.5)