##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 568687 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1208749276843 33.0 31.0 34.0 31.0 34.0 2 32.07749957357914 33.0 31.0 34.0 30.0 34.0 3 32.23424660665709 33.0 31.0 34.0 30.0 34.0 4 36.00418332052605 37.0 35.0 37.0 35.0 37.0 5 35.962529475792486 37.0 35.0 37.0 35.0 37.0 6 36.0393045735176 37.0 35.0 37.0 35.0 37.0 7 35.977601035367435 37.0 35.0 37.0 35.0 37.0 8 35.97109130330041 37.0 35.0 37.0 35.0 37.0 9 37.62259555783761 39.0 37.0 39.0 35.0 39.0 10-11 37.56126832510678 39.0 37.0 39.0 35.0 39.0 12-13 37.464689715080524 39.0 37.0 39.0 35.0 39.0 14-15 38.5387603374088 40.0 38.0 41.0 34.0 41.0 16-17 38.5104706103709 40.0 38.0 41.0 34.0 41.0 18-19 38.66700135575457 40.0 38.0 41.0 34.5 41.0 20-21 38.76899595032065 40.0 38.0 41.0 35.0 41.0 22-23 38.68238767546998 40.0 38.0 41.0 34.5 41.0 24-25 38.56519579311643 40.0 38.0 41.0 34.0 41.0 26-27 38.36864303210729 40.0 38.0 41.0 34.0 41.0 28-29 38.175280954198 40.0 37.5 41.0 34.0 41.0 30-31 37.897912208297356 40.0 37.0 41.0 33.0 41.0 32-33 37.68368452241743 40.0 36.0 41.0 33.0 41.0 34-35 37.52371779203675 40.0 36.0 41.0 33.0 41.0 36-37 37.28063416255339 40.0 35.0 41.0 33.0 41.0 38-39 37.05920743748318 39.5 35.0 41.0 32.5 41.0 40-41 36.8137789328752 39.0 35.0 41.0 31.5 41.0 42-43 36.439514179153036 39.0 35.0 41.0 31.0 41.0 44-45 35.85139804497025 39.0 35.0 41.0 29.5 41.0 46-47 35.258826911816165 39.0 35.0 41.0 26.0 41.0 48-49 34.672268752406865 39.0 35.0 40.5 22.5 41.0 50-51 34.303177318982144 38.5 34.5 40.0 20.0 41.0 52-53 34.042505807236665 38.0 34.0 40.0 18.5 41.0 54-55 33.791270945177224 38.0 34.0 40.0 15.5 41.0 56-57 33.49980569276245 38.0 33.5 40.0 11.5 41.0 58-59 33.180088519695374 37.5 33.0 40.0 8.0 41.0 60-61 32.812089954579584 37.0 33.0 40.0 6.5 41.0 62-63 32.456605303092914 36.5 32.5 40.0 2.0 41.0 64-65 32.064995331350985 36.0 32.0 39.0 2.0 40.5 66-67 31.675178085660477 35.5 31.5 39.0 2.0 40.0 68-69 31.23522429737272 35.0 31.0 38.5 2.0 40.0 70-71 30.740743150450072 35.0 30.5 38.0 2.0 40.0 72-73 30.42534381830427 35.0 30.0 37.0 2.0 39.5 74-75 30.006075398241915 35.0 30.0 37.0 2.0 39.0 76-77 28.63824828068867 33.5 28.0 35.0 2.0 37.5 78-79 29.314015442589685 34.0 29.0 36.0 2.0 38.0 80-81 29.11013879339602 34.0 29.0 35.5 2.0 37.0 82-83 28.767736030540526 34.0 29.0 35.0 2.0 37.0 84-85 28.38824872029781 34.0 29.0 35.0 2.0 36.5 86-87 28.04474869304204 34.0 29.0 35.0 2.0 36.0 88-89 27.685162488328377 34.0 27.5 35.0 2.0 36.0 90-91 27.273532716591024 34.0 26.5 35.0 2.0 35.0 92-93 26.837091405289726 33.0 25.5 35.0 2.0 35.0 94-95 26.396038594165155 33.0 25.0 35.0 2.0 35.0 96-97 25.81607808864982 33.0 23.5 35.0 2.0 35.0 98-99 25.200587493647646 33.0 19.0 35.0 2.0 35.0 100-101 22.538727806332833 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23202738234235198 1101 2 0.21183291457907671 1101 3 0.28234592599119424 1101 4 0.13040998004383653 1101 5 0.23415740893281622 1101 6 0.13982173113213037 1101 7 0.32692747066811734 1101 8 0.20936911396771762 1101 9 0.3122726797579176 1101 10-11 0.3201696490777195 1101 12-13 0.24594201925805237 1101 14-15 0.4095064761189917 1101 16-17 0.44040129391722616 1101 18-19 0.42590849005670606 1101 20-21 0.4342753706671161 1101 22-23 0.3146495590988607 1101 24-25 0.42352266644866887 1101 26-27 0.3996763584466194 1101 28-29 0.5527969579963141 1101 30-31 0.5630365642266781 1101 32-33 0.5779399822060398 1101 34-35 0.575716120193448 1101 36-37 0.5962610899289018 1101 38-39 0.528288324624576 1101 40-41 0.4110496582979195 1101 42-43 0.658461745371639 1101 44-45 0.9546729497736308 1101 46-47 0.49967695214563435 1101 48-49 -0.08440341096677173 1101 50-51 -0.1205231759365688 1101 52-53 -0.14716505353832332 1101 54-55 0.008297428778107019 1101 56-57 -0.1251735882206617 1101 58-59 -0.08510180442643644 1101 60-61 -0.10812444416123412 1101 62-63 0.2136892712538767 1101 64-65 0.10066448389558147 1101 66-67 0.1351706734461544 1101 68-69 0.10476481864104059 1101 70-71 0.11624597474772358 1101 72-73 0.28957783858794883 1101 74-75 0.1526090690024482 1101 76-77 0.09062901473454943 1101 78-79 -0.10872232863690812 1101 80-81 0.21119061346765378 1101 82-83 0.40715529375219717 1101 84-85 0.36937219266270915 1101 86-87 0.13214455617914211 1101 88-89 0.44693869188476043 1101 90-91 0.29197468458111686 1101 92-93 0.03182854784057909 1101 94-95 0.27432226880867105 1101 96-97 0.4075732060979185 1101 98-99 0.21050953257475769 1101 100-101 0.31558471049865133 1102 1 0.22593929703869264 1102 2 0.15819174628978772 1102 3 0.15352256020538135 1102 4 0.13564131986766625 1102 5 0.17300951834403122 1102 6 0.24453754012610318 1102 7 0.1725612212476264 1102 8 0.20977337915083893 1102 9 0.3109152388677856 1102 10-11 0.2581789212149417 1102 12-13 0.17665041054224417 1102 14-15 0.29710777421819756 1102 16-17 0.305893180797149 1102 18-19 0.40621354337149285 1102 20-21 0.2845804239819003 1102 22-23 0.22086096382764708 1102 24-25 0.2652908630179809 1102 26-27 0.3329141887665159 1102 28-29 0.23779000389339444 1102 30-31 0.31740652250205414 1102 32-33 0.3379742891137809 1102 34-35 0.3910309092523292 1102 36-37 0.44949335696645676 1102 38-39 0.49662212156476926 1102 40-41 0.546582342581651 1102 42-43 0.4199081987791544 1102 44-45 0.44660155431697746 1102 46-47 0.6355202530264918 1102 48-49 0.5623059075311474 1102 50-51 0.6836372320374693 1102 52-53 0.6462044411302017 1102 54-55 0.5443410078230784 1102 56-57 0.7126270608289431 1102 58-59 0.37516245148454885 1102 60-61 0.5799080083190589 1102 62-63 0.7123856087596678 1102 64-65 0.408789657748148 1102 66-67 0.4440162923613151 1102 68-69 0.4276280546169353 1102 70-71 0.26365904846121424 1102 72-73 0.5460136290376489 1102 74-75 0.5014361436431507 1102 76-77 0.45603188909709047 1102 78-79 0.5461835591702311 1102 80-81 0.5169041044273222 1102 82-83 0.6444580290326805 1102 84-85 0.5154046451430041 1102 86-87 0.18664061552082245 1102 88-89 0.4773939538226344 1102 90-91 0.46400528263396623 1102 92-93 0.4896820480723818 1102 94-95 0.9021294083543374 1102 96-97 0.8608045459217486 1102 98-99 1.0647997504699838 1102 100-101 1.234840157879269 1103 1 -0.02825292068794738 1103 2 0.07051473276089837 1103 3 0.10594819871846539 1103 4 0.128394828528684 1103 5 0.14886574226614613 1103 6 0.15807551901091443 1103 7 0.19961307672872408 1103 8 0.2872819927555952 1103 9 0.1099772252124609 1103 10-11 0.1986620733201434 1103 12-13 0.29234732228835725 1103 14-15 0.3627242791492975 1103 16-17 0.4982403090687413 1103 18-19 0.3907228839960979 1103 20-21 0.26056703733377873 1103 22-23 0.32935978637159025 1103 24-25 0.28659274220624553 1103 26-27 0.20630704026480373 1103 28-29 0.3385355943599535 1103 30-31 0.3135649733175825 1103 32-33 0.3277051337211887 1103 34-35 0.3083600595873861 1103 36-37 0.3516360899289026 1103 38-39 0.3952087791700265 1103 40-41 0.3834473855706477 1103 42-43 0.34124583628073424 1103 44-45 0.19209719219787047 1103 46-47 0.11351407335775576 1103 48-49 -6.761382394984139E-4 1103 50-51 -0.20935650926990235 1103 52-53 -0.023269220204987562 1103 54-55 -0.21578590455522573 1103 56-57 -0.09440843670550692 1103 58-59 -0.07745975897189084 1103 60-61 -0.2903327774945694 1103 62-63 -0.22622171359460452 1103 64-65 -0.27750028883169264 1103 66-67 -0.3336361447356673 1103 68-69 -0.06665752984380546 1103 70-71 -0.2625324343431821 1103 72-73 -0.2576702674726583 1103 74-75 -0.09113146130058425 1103 76-77 -0.056395606477568805 1103 78-79 0.15439888348430486 1103 80-81 0.06605993164947321 1103 82-83 -0.012403418369014929 1103 84-85 -0.025290686125170936 1103 86-87 -0.2311755195784322 1103 88-89 0.13167922218779182 1103 90-91 0.5626280936720214 1103 92-93 0.6584573357193655 1103 94-95 0.645477571838974 1103 96-97 0.9544103273100397 1103 98-99 0.8229451386353652 1103 100-101 1.1316566801956185 1104 1 0.2256570119719825 1104 2 0.17094402569018996 1104 3 0.13449407413934011 1104 4 0.14240998004383698 1104 5 0.11060185337725414 1104 6 0.11730321261360643 1104 7 0.1592237669644163 1104 8 0.2446283732269734 1104 9 0.3964208279060628 1104 10-11 0.2559474268554922 1104 12-13 0.16068275999879233 1104 14-15 0.2797286983412093 1104 16-17 0.2924753679912939 1104 18-19 0.35250108264929736 1104 20-21 0.28123833363007833 1104 22-23 0.2775384479877445 1104 24-25 0.3766337775597819 1104 26-27 0.18760228437255222 1104 28-29 0.29316732836668535 1104 30-31 0.21992545311556455 1104 32-33 0.15401405628011133 1104 34-35 0.13919760167492967 1104 36-37 0.0037425714103846985 1104 38-39 0.0540661024023521 1104 40-41 0.040827436075694834 1104 42-43 -0.20301973610983737 1104 44-45 -0.24199371689303462 1104 46-47 -0.3975823071136233 1104 48-49 -0.5728478554112186 1104 50-51 -0.46148613889953083 1104 52-53 -0.5546465350198062 1104 54-55 -0.5559247934441132 1104 56-57 -0.2779143289614012 1104 58-59 -0.1654721747968111 1104 60-61 -0.4753096293464196 1104 62-63 -0.4423107287461221 1104 64-65 -0.5102244049933056 1104 66-67 -0.3914959932205129 1104 68-69 -0.26353147765525264 1104 70-71 -0.23523550673375837 1104 72-73 -0.0877554947453838 1104 74-75 -0.18590944951607113 1104 76-77 -0.09181542970989298 1104 78-79 0.01246285654827517 1104 80-81 0.11059802087505943 1104 82-83 0.03315529375219839 1104 84-85 0.22018700747752362 1104 86-87 0.1368852969198855 1104 88-89 0.2851609141069815 1104 90-91 0.04419690680333943 1104 92-93 -0.08483811882608805 1104 94-95 0.09358152806793285 1104 96-97 0.149054687579401 1104 98-99 -0.09230528224005496 1104 100-101 0.21017730309123905 1105 1 0.2186475138547479 1105 2 -0.04195767722669075 1105 3 -0.0042458797751194766 1105 4 0.07129212470746893 1105 5 0.21551502147707424 1105 6 0.12545248479929683 1105 7 0.15310450658111563 1105 8 0.22079652924338689 1105 9 0.24590545669266106 1105 10-11 0.22616155600740484 1105 12-13 0.2931944218882947 1105 14-15 0.47355098800569095 1105 16-17 0.38502749523234314 1105 18-19 0.387819466283311 1105 20-21 0.29577663520935005 1105 22-23 0.41590297336289694 1105 24-25 0.3403835668027426 1105 26-27 0.31163083492614163 1105 28-29 0.29651471216930503 1105 30-31 0.4087300391887396 1105 32-33 0.3077497950537875 1105 34-35 0.31242527547923515 1105 36-37 0.3864189047999247 1105 38-39 0.47449773281881136 1105 40-41 0.38893736694738834 1105 42-43 0.5723919426402304 1105 44-45 0.3727255547306356 1105 46-47 0.38063496934341146 1105 48-49 0.49423695322139594 1105 50-51 0.4974909869364694 1105 52-53 0.5980438488390192 1105 54-55 0.6247395127437088 1105 56-57 0.741288728420713 1105 58-59 0.6754900013398739 1105 60-61 0.834690932672352 1105 62-63 0.7258632722655065 1105 64-65 0.8517449087817717 1105 66-67 0.9062784124446388 1105 68-69 0.7084957240532823 1105 70-71 0.7632717714093324 1105 72-73 0.7771610454670572 1105 74-75 0.5778088666756851 1105 76-77 0.6715162175671274 1105 78-79 0.5556438827945556 1105 80-81 0.7732361369881389 1105 82-83 0.8762913584967613 1105 84-85 0.9310373418786959 1105 86-87 0.80489518845026 1105 88-89 0.7451744025574953 1105 90-91 0.8983328029422673 1105 92-93 1.0523090738901466 1105 94-95 0.8479085105891464 1105 96-97 1.1834052748890151 1105 98-99 1.2339005290340417 1105 100-101 1.4191122775193854 1106 1 0.3281956671319932 1106 2 0.33594941943344736 1106 3 0.21935563472905528 1106 4 0.18771742340953068 1106 5 0.14046161605255492 1106 6 0.1225531227178891 1106 7 0.11393070691407559 1106 8 0.28724613662143383 1106 9 0.22574840791325101 1106 10-11 0.22528939017804106 1106 12-13 0.22913295776937304 1106 14-15 0.5715517835623558 1106 16-17 0.4670744330758012 1106 18-19 0.4102644771117241 1106 20-21 0.3226119402463965 1106 22-23 0.33932917074934466 1106 24-25 0.36908253699883176 1106 26-27 0.22128800893205636 1106 28-29 0.2505639482875779 1106 30-31 0.3737550108286172 1106 32-33 0.36006943204423436 1106 34-35 0.19370317520962743 1106 36-37 0.12963973070560542 1106 38-39 0.48832715957602346 1106 40-41 0.5069331534435548 1106 42-43 0.4376850463425086 1106 44-45 0.35897715689336707 1106 46-47 0.20814944405501734 1106 48-49 0.2273247443730284 1106 50-51 0.25532795674951814 1106 52-53 0.21667313416394762 1106 54-55 0.32189613427972574 1106 56-57 0.3542179975398554 1106 58-59 0.22955838974831977 1106 60-61 0.22450338755397325 1106 62-63 0.20966338128624074 1106 64-65 0.3089557460315042 1106 66-67 0.526626984125766 1106 68-69 0.5037680548869972 1106 70-71 0.4747896640681155 1106 72-73 0.4322445052546122 1106 74-75 0.42458317903480847 1106 76-77 0.6632439014659433 1106 78-79 0.3756919108452905 1106 80-81 0.3927051765744487 1106 82-83 0.6443527047554376 1106 84-85 0.6502912865138413 1106 86-87 0.662487598250344 1106 88-89 0.9463043876776389 1106 90-91 1.12139216030927 1106 92-93 1.3254531433098329 1106 94-95 1.469338450038446 1106 96-97 1.6012301640267204 1106 98-99 1.777007914451783 1106 100-101 1.9740248399484877 1107 1 0.34162202220834814 1107 2 0.15758835846517627 1107 3 0.1498300130933714 1107 4 0.22683209059660214 1107 5 0.09141704042360033 1107 6 0.15173462895457135 1107 7 0.11666281405170054 1107 8 0.05712790793756284 1107 9 0.14529110521855415 1107 10-11 0.2772986273021729 1107 12-13 0.26771421356290404 1107 14-15 0.3501697927019052 1107 16-17 0.329155062761437 1107 18-19 0.3573624264051176 1107 20-21 0.3789453874175308 1107 22-23 0.3738756897521256 1107 24-25 0.42716420748720196 1107 26-27 0.3326311323159743 1107 28-29 0.4525122008773863 1107 30-31 0.52132132134561 1107 32-33 0.6313302669631611 1107 34-35 0.6794246629070173 1107 36-37 0.846113686243811 1107 38-39 0.923625008041661 1107 40-41 0.8780915343448257 1107 42-43 0.8741066364939201 1107 44-45 0.7543211909796597 1107 46-47 0.8135697494655716 1107 48-49 1.1279784985809655 1107 50-51 0.8747096548842066 1107 52-53 1.0747076432791047 1107 54-55 1.128173475679283 1107 56-57 1.0586723079267415 1107 58-59 0.998801043144752 1107 60-61 0.9835103967097893 1107 62-63 0.851290611287375 1107 64-65 1.0278772142138344 1107 66-67 1.2436798861764693 1107 68-69 1.2105202625271367 1107 70-71 1.2424938809453785 1107 72-73 1.3625627632110628 1107 74-75 1.626344412386029 1107 76-77 1.4545385624732425 1107 78-79 1.5011135172592418 1107 80-81 1.4130398596988094 1107 82-83 1.3014635014573912 1107 84-85 1.3000790603344008 1107 86-87 1.3929619766146573 1107 88-89 1.2297393619015082 1107 90-91 1.4225776996564896 1107 92-93 1.6547598711236624 1107 94-95 1.7739537595959405 1107 96-97 1.8950438928650897 1107 98-99 1.840968494048795 1107 100-101 1.9045528847030049 1108 1 -0.04281594047573378 1108 2 -0.21631850497928085 1108 3 -0.014915903661801622 1108 4 0.039045100969175905 1108 5 0.05053175172987068 1108 6 0.027514685916564474 1108 7 0.07411569359135939 1108 8 0.0923302075534167 1108 9 -0.0065538186647984276 1108 10-11 0.004342099130290933 1108 12-13 -0.018060083791368697 1108 14-15 0.03385768537240352 1108 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-1.0519283225553622 2316 52-53 -0.8615911456987035 2316 54-55 -0.6940736065300896 2316 56-57 -0.9492262512966647 2316 58-59 -0.7344913915000504 2316 60-61 -0.5630268977818247 2316 62-63 -0.5314549537610844 2316 64-65 -0.5199351570379918 2316 66-67 -0.4704307628195572 2316 68-69 -0.5697725841117993 2316 70-71 -0.46280249921995775 2316 72-73 -0.6047393367561575 2316 74-75 -0.5438242044864836 2316 76-77 -1.1811112605497094 2316 78-79 -0.7831113172664743 2316 80-81 -0.9122851495485058 2316 82-83 -0.7904593082825109 2316 84-85 -0.63291625736721 2316 86-87 -0.7380265988178643 2316 88-89 -0.8009511944108709 2316 90-91 -0.9718134662267843 2316 92-93 -1.0018219608368604 2316 94-95 -0.8701780304133528 2316 96-97 -1.0323669494975327 2316 98-99 -0.8430440281673128 2316 100-101 -0.6856708251087724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 4.0 5 23.0 6 45.0 7 109.0 8 247.0 9 446.0 10 837.0 11 1461.0 12 2313.0 13 3200.0 14 4037.0 15 5949.0 16 8297.0 17 12184.0 18 10191.0 19 5168.0 20 3929.0 21 3813.0 22 3957.0 23 4103.0 24 4562.0 25 5136.0 26 6011.0 27 7034.0 28 8745.0 29 10484.0 30 13034.0 31 16209.0 32 20269.0 33 26317.0 34 36267.0 35 52611.0 36 77550.0 37 114528.0 38 91466.0 39 8147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60694826961566 21.038441651629785 16.097199798804787 18.25741027994977 2 15.391473468009679 23.56314360474931 39.86568707444713 21.179695852793877 3 18.983953927990505 29.594408053809296 31.4386952125555 19.9829428056447 4 12.047639814026772 18.95182561844539 38.70497499490051 30.29555957262733 5 11.603484597502261 40.64912410273725 32.956315920896685 14.791075378863813 6 27.074694998646002 39.48910294960663 17.8423594039593 15.593842647788058 7 25.0455874672711 37.143455011280366 21.59711757082543 16.2138399506231 8 26.91216785331826 33.262761413571965 20.190368339701802 19.634702393407974 9 28.111069885543365 16.723610703251527 21.251057259969016 33.91426215123609 10-11 24.086272413471736 28.83317185024451 26.651743401906497 20.42881233437726 12-13 26.911535462043606 27.161409953304595 27.75989540791879 18.167159176733012 14-15 23.329968858088897 27.072801031147186 29.06308742770628 20.53414268305764 16-17 19.395115414982232 30.490146600854253 28.93155637459622 21.1831816095673 18-19 19.29233479928326 27.48533024317419 34.48100624772502 18.741328709817527 20-21 21.438971635570717 25.78085708759034 34.60055919953576 18.17961207730318 22-23 20.55498015604366 26.670558672872772 34.41655954857417 18.357901622509395 24-25 20.16791871531368 26.695980783163463 34.76527604859074 18.370824452932116 26-27 19.90208801527765 26.639779557356235 35.08275391524845 18.375378512117663 28-29 19.264565979274483 27.227781968836112 34.83587170789437 18.671780343995035 30-31 19.694969833775794 26.668331576091788 34.718197958316885 18.91850063181554 32-33 19.44695023413747 27.277586986071327 34.37987420056516 18.895588579226036 34-35 19.916243753011393 26.920080749251767 34.49788455248525 18.665790945251583 36-37 20.079009818636155 26.795635731851963 34.238613872703276 18.886740576808606 38-39 19.563722688706203 27.403174044929003 34.08045016925309 18.952653097111707 40-41 19.721047929884783 27.51958977533306 33.77187583529114 18.987486459491016 42-43 19.64971051000356 27.886437272771236 33.01962025594696 19.44423196127824 44-45 20.04818133061361 27.68166911383279 32.64267564644751 19.627473909106097 46-47 20.641934842892486 28.37052719685873 31.800269744165067 19.187268216083716 48-49 20.621151761386567 28.85346997593573 30.683391786848794 19.841986475828907 50-51 20.248608195032055 29.233619255617338 30.694794667004288 19.822977882346322 52-53 20.726594921572765 29.659034958148695 29.73429696841809 19.88007315186045 54-55 20.19383961763144 29.305427087253328 29.95598470807117 20.544748587044065 56-57 20.527111254131245 29.459392686102753 29.792442030686612 20.221054029079394 58-59 21.015565033670168 28.87527838664553 29.9181347633153 20.191021816369002 60-61 20.363842902724645 29.460443008759768 30.538554669194156 19.637159419321428 62-63 20.31829459790711 29.194178871681608 30.760066609576093 19.727459920835187 64-65 20.290476898039426 29.64705447914533 30.24413318081787 19.818335441997377 66-67 20.33730359345615 29.375587428222754 29.777038843018076 20.510070135303017 68-69 20.213228014707564 29.567055339756315 29.819478904916064 20.40023774062006 70-71 20.63303938032478 29.711615393407598 29.531287092151192 20.124058134116428 72-73 20.416503278604928 29.652779121027912 29.524764940995663 20.405952659371497 74-75 20.124919661218723 30.46807066471714 29.137985758384904 20.26902391567923 76-77 20.176390320073434 29.795210680134065 29.297744609465397 20.730654390327107 78-79 20.157134579894475 30.0251721297134 28.99859236536252 20.819100925029606 80-81 20.202355924498573 30.396473059510086 28.759794003884632 20.641377012106712 82-83 20.314151316657142 29.914010638765554 28.91361498219546 20.858223062381853 84-85 19.878645706482985 30.294434953412857 28.519314078311414 21.30760526179274 86-87 19.89013037346529 30.1120677224561 28.311475467491043 21.68632643658757 88-89 20.041816245910475 29.891054435854354 28.264652171657623 21.802477146577548 90-91 20.016757929683514 29.561480324818966 28.448388038390256 21.97337370710726 92-93 19.924439849055542 30.261603063905213 28.275885110588266 21.538071976450976 94-95 19.84847525699595 30.019553848696823 28.081489609380224 22.050481284927006 96-97 19.761797369501586 30.193141782967615 28.080161709094202 21.964899138436593 98-99 19.66834749655994 30.637323216114865 27.64295480223505 22.051374485090143 100-101 19.185603829225276 30.609095816796472 27.621909724115014 22.583390629863246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1606.0 1 1094.0 2 621.5 3 673.0 4 634.0 5 668.5 6 992.5 7 1747.5 8 2695.0 9 3096.5 10 2701.0 11 2309.5 12 2322.0 13 2400.0 14 2486.5 15 2531.5 16 2567.5 17 2685.5 18 2723.0 19 2753.0 20 2908.0 21 3101.5 22 3357.0 23 3690.0 24 4079.0 25 4565.0 26 5216.0 27 5986.5 28 6808.0 29 7715.0 30 8853.0 31 9864.5 32 11069.5 33 12441.0 34 13845.5 35 14722.5 36 16105.0 37 17801.5 38 18465.0 39 18805.0 40 18648.0 41 18282.0 42 17763.5 43 17758.5 44 18539.5 45 19036.0 46 18648.0 47 19435.0 48 22795.0 49 21834.0 50 17262.0 51 14857.0 52 13393.0 53 12890.0 54 12199.5 55 11014.5 56 10042.5 57 9098.5 58 8507.0 59 8064.5 60 7462.0 61 6500.0 62 5251.0 63 3659.0 64 2266.0 65 1588.0 66 1074.5 67 809.5 68 602.5 69 388.5 70 284.0 71 216.5 72 178.0 73 148.5 74 100.5 75 74.0 76 50.5 77 25.0 78 14.0 79 6.0 80 5.0 81 2.5 82 3.5 83 3.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014946710580688497 2 0.0026376548083567935 3 0.002110123846685435 4 5.275309616713588E-4 5 0.002285967500575888 6 1.7584365389045292E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.637654808356794E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0029893421161376996 22-23 0.0 24-25 0.0033410294239186056 26-27 0.002285967500575888 28-29 0.0028134984622472468 30-31 0.01538631971541463 32-33 7.033746155618117E-4 34-35 0.002285967500575888 36-37 0.004132325866425643 38-39 0.002110123846685435 40-41 0.004044404039480418 42-43 0.0025497329814115673 44-45 3.5168730778090585E-4 46-47 0.0 48-49 2.637654808356794E-4 50-51 1.7584365389045292E-4 52-53 0.0012309055772331704 54-55 0.0036927167316995113 56-57 4.3960913472613236E-4 58-59 0.0014946710580688498 60-61 0.0013188274041783968 62-63 0.0 64-65 2.637654808356794E-4 66-67 8.792182694522646E-5 68-69 0.0 70-71 3.5168730778090585E-4 72-73 0.0 74-75 2.637654808356794E-4 76-77 8.792182694522647E-4 78-79 4.3960913472613236E-4 80-81 0.006242449713111079 82-83 0.002110123846685435 84-85 0.003780638558644738 86-87 0.002285967500575888 88-89 0.0014946710580688498 90-91 1.7584365389045292E-4 92-93 1.7584365389045292E-4 94-95 1.7584365389045292E-4 96-97 0.002022202019740209 98-99 0.0034289512508638323 100-101 0.0014067492311236234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 568687.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.462124724986914 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35293546137241 40.05637223293396 2 12.901143663592682 13.536428159487476 3 4.4130199591267925 6.945492105286993 4 2.0885918481952017 4.382878641584304 5 1.1099529221394122 2.9115244320070763 6 0.6634169281067727 2.088255697620311 7 0.4725773291319155 1.7354687548183823 8 0.30702981308220234 1.2885949078566332 9 0.22987050443754997 1.0853545566958562 >10 1.3193939672748491 12.687625503951391 >50 0.09224294727474988 3.2862820802831587 >100 0.044438206939447486 4.223932955217322 >500 0.002693224662996807 0.9750934038198186 >1k 0.002019918497247605 2.3493375217553387 >5k 6.733061657492017E-4 2.447359046681983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7833 1.3773833409239176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6024 1.0592821710360885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4112 0.7230691047975424 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3067 0.5393124864820191 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2165 0.3807015106728306 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1503 0.2642930117973507 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1320 0.23211362313539785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.19958254716566404 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 995 0.17496443562100067 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 827 0.14542270176740457 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 821 0.14436763984406184 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 689 0.12115627753052206 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.10286853752591496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7584365389045292E-4 0.0 12-13 0.0 0.0 0.0 5.275309616713588E-4 0.0 14-15 0.0 0.0 0.0 5.275309616713588E-4 0.0 16-17 0.0 0.0 0.0 5.275309616713588E-4 0.0 18-19 0.0 0.0 0.0 5.275309616713588E-4 0.0 20-21 0.0 0.0 0.0 8.792182694522645E-4 0.0 22-23 0.0 0.0 0.0 0.0012309055772331704 0.0 24-25 0.0 0.0 0.0 0.0012309055772331704 0.0 26-27 0.0 0.0 0.0 0.00272557663530202 0.0 28-29 0.0 0.0 0.0 0.006594137020891984 0.0 30-31 0.0 0.0 0.0 0.012924508560948289 0.0 32-33 0.0 0.0 0.0 0.01916695827405937 0.0 34-35 0.0 0.0 0.0 0.02532148616022522 0.0 36-37 0.0 0.0 0.0 0.030245108469157903 0.0 38-39 0.0 0.0 0.0 0.036399636355323756 0.0 40-41 0.0 0.0 0.0 0.04633480280013434 0.0 42-43 0.0 0.0 0.0 0.06286410626583692 0.0 44-45 0.0 0.0 0.0 0.08827351425300736 0.0 46-47 0.0 0.0 0.0 0.11526551512519188 0.0 48-49 0.0 0.0 0.0 0.18023974523771424 0.0 50-51 0.0 0.0 0.0 0.24635695910052452 0.0 52-53 0.0 0.0 0.0 0.30851769075079966 0.0 54-55 0.0 0.0 0.0 0.40655052779472717 0.0 56-57 0.0 0.0 0.0 0.5105620490709301 0.0 58-59 0.0 0.0 0.0 0.6478080209324286 0.0 60-61 0.0 0.0 0.0 0.7959562993351351 0.0 62-63 0.0 0.0 0.0 1.0096942606389807 0.0 64-65 0.0 0.0 0.0 1.1602164283692082 0.0 66-67 0.0 0.0 0.0 1.3841533215987 0.0 68-69 0.0 0.0 0.0 1.6658548551312058 0.0 70-71 0.0 0.0 0.0 1.8449516166186322 0.0 72-73 0.0 0.0 0.0 2.1161904527446556 0.0 74-75 0.0 0.0 0.0 2.43420370080554 0.0 76-77 0.0 0.0 0.0 2.664558887402033 0.0 78-79 0.0 0.0 0.0 2.882253330918414 0.0 80-81 0.0 0.0 0.0 3.1986839860942835 0.0 82-83 0.0 0.0 0.0 3.5980249240795024 0.0 84-85 0.0 0.0 0.0 4.122214856326942 0.0 86-87 0.0 0.0 0.0 4.79050866293761 0.0 88-89 0.0 0.0 0.0 5.503906366771176 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 55 0.002578874 34.544525 3 CGTATAG 125 5.63341E-9 34.205097 1 GTATCAA 8565 0.0 29.341795 1 GTAAGGT 200 2.3646862E-11 28.49673 4 GGTATCA 5955 0.0 27.682547 1 GTACATG 5170 0.0 25.085943 1 GTATAGT 120 1.7076086E-4 23.75354 1 GACCGGG 50 0.0016547397 23.747274 90-91 AGTACTT 5935 0.0 23.72831 12-13 TACATGG 5385 0.0 23.641184 2 ATCAACG 10765 0.0 23.252975 3 TATCAAC 10795 0.0 23.234398 2 TCAACGC 10885 0.0 22.994606 4 ACATGGG 5265 0.0 22.824648 3 CAACGCA 11035 0.0 22.682035 5 AGAGTAC 8920 0.0 22.36487 10-11 AACGCAG 11230 0.0 22.332438 6 GAGTACT 5895 0.0 22.277895 12-13 ATGGGAT 1440 0.0 21.43851 5 CATGGGG 1120 0.0 21.202925 4 >>END_MODULE