FastQCFastQC Report
Thu 26 May 2016
SRR937086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466257
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95052.0385752921671956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65311.4007296405201424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.9859369403569277No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.329860999405907No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14120.3028372764376728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.27945961133023206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.25651089420641404No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10840.23248980712353914No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10600.22734243132006599No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10440.22391084745108383No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.15699496200593235No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7310.15678048801412095No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.13554756282479405No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.13082913500494364No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6070.1301857130295095No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5380.11538700759452405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.040.1741141
GTATCAA62850.039.8656121
TATCAAC79350.031.4595242
ATCAACG79400.031.3199423
TCAACGC79800.031.2225364
CAACGCA80750.030.8029465
AACGCAG81550.030.5525236
ACGCAGA90650.027.223217
CGCAGAG90750.027.1408168
GTACATG37300.027.0221671
GTCGGGA1606.163282E-826.7462142
GCAGAGT92150.026.0576869
TACATGG38600.025.7453482
GAGTACT53800.025.49509812-13
ACATGGG38100.025.3344143
AGTACTT55000.024.33373812-13
CATGGGG9850.024.136464
AGAGTAC75000.024.12180710-11
TAAGGTA1000.001642322723.7744124
CGATTCG500.001659029323.73616438-39
Produced by FastQC (version 0.11.5)