##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 466257 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02807893500795 33.0 31.0 34.0 30.0 34.0 2 32.09714384985105 33.0 31.0 34.0 30.0 34.0 3 32.0629073665382 34.0 31.0 34.0 30.0 34.0 4 35.68319617721557 37.0 35.0 37.0 35.0 37.0 5 35.710149123766506 37.0 35.0 37.0 35.0 37.0 6 35.81551161698377 37.0 35.0 37.0 35.0 37.0 7 35.77283558209314 37.0 35.0 37.0 35.0 37.0 8 35.739735810936885 37.0 35.0 37.0 35.0 37.0 9 37.39073515250173 39.0 37.0 39.0 35.0 39.0 10-11 37.2347878959458 39.0 37.0 39.0 34.0 39.0 12-13 37.17895602639746 39.0 37.0 39.0 34.0 39.0 14-15 38.252521677958725 40.0 38.0 41.0 33.5 41.0 16-17 38.24242424242424 40.0 38.0 41.0 33.5 41.0 18-19 38.36150449215776 40.0 38.0 41.0 34.0 41.0 20-21 38.36560309014128 40.0 38.0 41.0 34.0 41.0 22-23 38.26030279438164 40.0 38.0 41.0 34.0 41.0 24-25 38.11973225066862 40.0 38.0 41.0 34.0 41.0 26-27 38.11047448081209 40.0 38.0 41.0 34.0 41.0 28-29 38.014518816875665 40.0 38.0 41.0 33.5 41.0 30-31 37.689074909331126 40.0 37.0 41.0 33.0 41.0 32-33 37.28027354012916 39.5 35.5 41.0 33.0 41.0 34-35 37.01568770013104 39.0 35.0 41.0 32.5 41.0 36-37 36.77851592576626 39.0 35.0 41.0 31.5 41.0 38-39 36.54325940414836 39.0 35.0 41.0 31.0 41.0 40-41 36.27302431920593 39.0 35.0 41.0 30.5 41.0 42-43 35.86776498797873 39.0 35.0 41.0 30.0 41.0 44-45 35.30847258057251 39.0 35.0 41.0 27.0 41.0 46-47 34.520916576051405 38.0 35.0 40.0 21.5 41.0 48-49 33.867400382192656 38.0 34.0 40.0 12.0 41.0 50-51 33.068629532639726 38.0 33.5 39.5 7.0 40.5 52-53 33.24502152246508 38.0 33.5 40.0 3.5 40.5 54-55 33.579091573960284 39.0 34.0 40.5 2.0 41.0 56-57 33.44520511220207 38.5 34.0 41.0 2.0 41.0 58-59 33.218592964824126 38.0 34.0 40.5 2.0 41.0 60-61 32.937449732658166 38.0 34.0 40.0 2.0 41.0 62-63 32.636498754978476 37.5 33.0 40.0 2.0 41.0 64-65 32.32091528920745 37.0 33.0 40.0 2.0 41.0 66-67 31.942374055510157 36.5 32.5 39.5 2.0 41.0 68-69 31.555913369665227 36.0 32.0 39.0 2.0 41.0 70-71 31.125268896767235 35.5 31.5 39.0 2.0 41.0 72-73 30.745953411959498 35.0 31.0 38.5 2.0 40.0 74-75 30.33656760112985 35.0 31.0 37.5 2.0 40.0 76-77 29.91182974196634 35.0 30.5 37.0 2.0 39.0 78-79 29.481461940517782 35.0 30.0 36.5 2.0 39.0 80-81 29.05944575631036 35.0 29.5 36.0 2.0 38.5 82-83 28.660497322292212 34.5 29.0 35.5 2.0 37.0 84-85 28.300246430616593 34.0 29.0 35.0 2.0 37.0 86-87 27.985693512376223 34.0 29.0 35.0 2.0 36.5 88-89 27.64055660290355 34.0 27.5 35.0 2.0 36.0 90-91 27.288921131478993 34.0 26.5 35.0 2.0 36.0 92-93 26.967678769434023 34.0 26.0 35.0 2.0 35.0 94-95 26.61937086199242 34.0 25.0 35.0 2.0 35.0 96-97 26.224281887456918 34.0 24.5 35.0 2.0 35.0 98-99 25.73741091286565 34.0 21.5 35.0 2.0 35.0 100-101 23.891263830891546 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21398482855294532 1101 2 0.08070763934215108 1101 3 0.17668188273983532 1101 4 0.03905063430318023 1101 5 0.14380780694322226 1101 6 -0.08399919982760196 1101 7 0.03670897930415151 1101 8 0.17780554703104912 1101 9 0.05385013932700389 1101 10-11 0.19159430807558664 1101 12-13 0.3408469621249566 1101 14-15 0.49018873826007336 1101 16-17 0.4392420589928818 1101 18-19 0.1829939556087794 1101 20-21 0.11237581259023699 1101 22-23 0.1680100985748254 1101 24-25 0.32235000831466465 1101 26-27 0.4587337362548638 1101 28-29 0.4681587369668492 1101 30-31 0.3238914609376451 1101 32-33 0.577472529192228 1101 34-35 0.5327527488376944 1101 36-37 0.35566787875158923 1101 38-39 0.5256339870939826 1101 40-41 0.5738760148955606 1101 42-43 0.5394826748789328 1101 44-45 0.6036063022711815 1101 46-47 0.8379007679987751 1101 48-49 1.1076609113360618 1101 50-51 1.0014765900740201 1101 52-53 1.249989206805303 1101 54-55 1.2520816559938126 1101 56-57 1.1510939093668924 1101 58-59 1.1141548175205571 1101 60-61 1.0863319293414548 1101 62-63 0.9380201750422756 1101 64-65 1.187686639689769 1101 66-67 1.0280563138385475 1101 68-69 1.140075093083226 1101 70-71 1.198548057721485 1101 72-73 1.1399870857151626 1101 74-75 1.1556919350294095 1101 76-77 1.1398057701003168 1101 78-79 0.9686307366623126 1101 80-81 0.7704626172857161 1101 82-83 0.831249408408393 1101 84-85 1.0042527709257918 1101 86-87 1.0175687450752449 1101 88-89 1.0192829293494334 1101 90-91 1.0220177963995845 1101 92-93 1.196581745973429 1101 94-95 1.1543102840953274 1101 96-97 1.1237388916153428 1101 98-99 1.2416594189119614 1101 100-101 1.1839354821792476 1102 1 0.3531297784757115 1102 2 0.2049381350410755 1102 3 0.24416271024856684 1102 4 0.27442042670122646 1102 5 0.23607811075869023 1102 6 0.11405388068100564 1102 7 0.23570426375462006 1102 8 0.3196502736333642 1102 9 0.43253232215987225 1102 10-11 0.3456706990721088 1102 12-13 0.4198539335281595 1102 14-15 0.6662087419745291 1102 16-17 0.7008576669635644 1102 18-19 0.5584271202815714 1102 20-21 0.4933965184278364 1102 22-23 0.5148895010000913 1102 24-25 0.5582135518168556 1102 26-27 0.5413396549263538 1102 28-29 0.5461589543985284 1102 30-31 0.5972008163886073 1102 32-33 0.829594752974721 1102 34-35 0.820808954926683 1102 36-37 0.6964557967979701 1102 38-39 0.6278823665485049 1102 40-41 0.5428761462605323 1102 42-43 0.766499466946307 1102 44-45 0.8461094391761321 1102 46-47 1.0848578569509755 1102 48-49 1.247256579009857 1102 50-51 1.5048886819932648 1102 52-53 1.3134260315780182 1102 54-55 1.4321286755943632 1102 56-57 1.2561734304736163 1102 58-59 1.3044777488619772 1102 60-61 1.177589816902099 1102 62-63 1.2156306008912807 1102 64-65 1.3083295126870205 1102 66-67 1.1324858048219255 1102 68-69 1.1088589252259595 1102 70-71 1.2453864652156277 1102 72-73 1.2784378397048073 1102 74-75 1.245836617692401 1102 76-77 1.1985564892102971 1102 78-79 1.4661914250147703 1102 80-81 1.3921756618275047 1102 82-83 1.3899446371419906 1102 84-85 1.5094779259591071 1102 86-87 1.478107160270092 1102 88-89 1.4401887587833393 1102 90-91 1.4961083204552281 1102 92-93 1.4494661130887003 1102 94-95 1.3038387291417344 1102 96-97 1.118655293664638 1102 98-99 1.3729054836250647 1102 100-101 1.0508491753922087 1103 1 0.2983194176986572 1103 2 0.38291487615380504 1103 3 0.4033737582200203 1103 4 0.21207735811393746 1103 5 0.19388833938297978 1103 6 0.2519552947156285 1103 7 0.35758215459642884 1103 8 0.36342587823227746 1103 9 0.26152663959081934 1103 10-11 0.3533025214587795 1103 12-13 0.35238994515760425 1103 14-15 0.4634533567492056 1103 16-17 0.5477421654438075 1103 18-19 0.5564943860407894 1103 20-21 0.6501462210840998 1103 22-23 0.6817654095503798 1103 24-25 0.5424708532573206 1103 26-27 0.6492589095847379 1103 28-29 0.49846832402982244 1103 30-31 0.44203694695554674 1103 32-33 0.6745465172141749 1103 34-35 0.588666097783829 1103 36-37 0.49379952614910394 1103 38-39 0.6109833312637107 1103 40-41 0.5340714680525309 1103 42-43 0.7110283423262374 1103 44-45 1.1087795858657046 1103 46-47 1.1757459235559722 1103 48-49 1.0577990661249643 1103 50-51 1.0468802242160749 1103 52-53 0.9847620960685717 1103 54-55 1.1083543925231325 1103 56-57 1.2746417798855347 1103 58-59 1.3804325526151189 1103 60-61 1.218771923417222 1103 62-63 1.1741676697428716 1103 64-65 0.9282911883841329 1103 66-67 0.940418274757171 1103 68-69 0.9557599428022812 1103 70-71 0.9953864652156277 1103 72-73 1.1256196819144009 1103 74-75 1.126667858606897 1103 76-77 1.2136549430229024 1103 78-79 1.0794959049936246 1103 80-81 1.0215201840952446 1103 82-83 1.2014452318294495 1103 84-85 0.9178134618385876 1103 86-87 1.027234951997329 1103 88-89 1.1043059782890872 1103 90-91 0.9853709080064377 1103 92-93 1.117597472940691 1103 94-95 1.339453900279569 1103 96-97 1.2624375059019854 1103 98-99 1.346260181655989 1103 100-101 1.144528969233889 1104 1 0.3088615401417911 1104 2 0.4309287310761931 1104 3 0.32960959887817864 1104 4 0.24446093685880754 1104 5 0.22905518170905026 1104 6 0.15467920929399526 1104 7 0.3825573405736904 1104 8 0.3142096943731616 1104 9 0.3429217504608957 1104 10-11 0.5211425247332144 1104 12-13 0.5088410794398399 1104 14-15 0.6845309504541177 1104 16-17 0.6626809745753732 1104 18-19 0.740178270739996 1104 20-21 0.6694717401320602 1104 22-23 0.48492625558064617 1104 24-25 0.6594664123146927 1104 26-27 0.5382801115043705 1104 28-29 0.565769325800062 1104 30-31 0.5558143574971552 1104 32-33 0.8571419105036497 1104 34-35 0.7737390561269635 1104 36-37 0.814007861614229 1104 38-39 1.0280904255160692 1104 40-41 0.8973500399927303 1104 42-43 0.9820921220391128 1104 44-45 1.0865352214628246 1104 46-47 1.2314956140202185 1104 48-49 1.223951971667951 1104 50-51 1.196659711972785 1104 52-53 1.2030308447255393 1104 54-55 1.3053879656400866 1104 56-57 1.3256102942162826 1104 58-59 1.3903402546831245 1104 60-61 1.2503228254197438 1104 62-63 1.044436377469502 1104 64-65 1.1155807808201992 1104 66-67 0.9464107333407767 1104 68-69 1.0114380051596612 1104 70-71 1.076758552765643 1104 72-73 0.9166868895438185 1104 74-75 0.8927560430390393 1104 76-77 0.8845289082525731 1104 78-79 1.123272189950697 1104 80-81 1.0289950886763748 1104 82-83 1.151585674081847 1104 84-85 1.0351159984968277 1104 86-87 0.889092473460181 1104 88-89 1.1575653129663017 1104 90-91 1.1171768572323408 1104 92-93 1.0567980009421625 1104 94-95 1.0101513533887392 1104 96-97 1.3235132577412898 1104 98-99 1.3119336905122907 1104 100-101 1.2158667264555376 1105 1 0.2310444358054511 1105 2 0.4536466102362837 1105 3 0.2781021562622925 1105 4 0.22574524653217054 1105 5 0.31436438354162277 1105 6 0.1920931258505405 1105 7 0.2951466257682114 1105 8 0.47911733840830095 1105 9 0.4456892276556985 1105 10-11 0.4273428612081389 1105 12-13 0.428926828154772 1105 14-15 0.6354350085280629 1105 16-17 0.558027374933971 1105 18-19 0.41929150212181554 1105 20-21 0.4561812714539215 1105 22-23 0.45659366932255807 1105 24-25 0.46006572190074024 1105 26-27 0.4442927089394786 1105 28-29 0.6615584410107047 1105 30-31 0.3267378007184618 1105 32-33 0.6214551931134835 1105 34-35 0.996099212372414 1105 36-37 0.7936316267570476 1105 38-39 0.6283576261960064 1105 40-41 1.0199509097047397 1105 42-43 1.1035068885150352 1105 44-45 1.3893553820160918 1105 46-47 1.7258139612181083 1105 48-49 1.9937256951880187 1105 50-51 2.01068309057694 1105 52-53 1.6230321836396229 1105 54-55 1.5855892102480453 1105 56-57 1.81702814601595 1105 58-59 1.5397339594583883 1105 60-61 1.7816985305326511 1105 62-63 1.9470041818250436 1105 64-65 1.6042014664606334 1105 66-67 1.4880364078915136 1105 68-69 1.4343500268617149 1105 70-71 1.5529331588047306 1105 72-73 1.5195824367812882 1105 74-75 1.547387521491256 1105 76-77 1.5470614351327292 1105 78-79 1.364861772034864 1105 80-81 1.2575724633569294 1105 82-83 1.4012756863649294 1105 84-85 1.3054223769670905 1105 86-87 1.5766335868545625 1105 88-89 1.4865816186112788 1105 90-91 1.5787471123827963 1105 92-93 1.4969732822596562 1105 94-95 1.9229865912788462 1105 96-97 1.7266573772888556 1105 98-99 1.9431123424004504 1105 100-101 1.9086659883063817 1106 1 -0.11927494446092268 1106 2 0.02927178011940157 1106 3 0.04572922883635755 1106 4 -0.10889731938247849 1106 5 -0.06687372463995445 1106 6 -0.3085320440325816 1106 7 -0.07782157211164531 1106 8 0.007895539006291585 1106 9 -0.14021726867483153 1106 10-11 0.026612363790363247 1106 12-13 -0.07350589812954667 1106 14-15 -0.16047760359479923 1106 16-17 0.19458282478446876 1106 18-19 0.21395377447843344 1106 20-21 0.2528277786569575 1106 22-23 -0.024039654950342992 1106 24-25 -0.01212780895004073 1106 26-27 -0.29451199342529577 1106 28-29 -0.006775345344280481 1106 30-31 0.2431179311887064 1106 32-33 0.21524766389358518 1106 34-35 -0.08097675935903226 1106 36-37 0.10463325763500109 1106 38-39 0.19836985778067628 1106 40-41 0.14871551965309493 1106 42-43 0.3114404302383278 1106 44-45 0.2705629112359844 1106 46-47 0.5332650348794346 1106 48-49 0.6862032706161258 1106 50-51 0.5393491683154537 1106 52-53 0.39295607600167415 1106 54-55 0.3629709715962335 1106 56-57 0.5305304564263906 1106 58-59 0.5905018311626549 1106 60-61 0.7470865435796696 1106 62-63 0.647238620531219 1106 64-65 0.646685837213564 1106 66-67 0.7702020779105396 1106 68-69 0.5893242046274132 1106 70-71 0.6499194322485984 1106 72-73 0.7601335376239113 1106 74-75 0.9218303048563037 1106 76-77 0.7920770643798072 1106 78-79 0.7055589723610396 1106 80-81 0.5714485166322731 1106 82-83 0.2603678309694395 1106 84-85 0.35612569308559827 1106 86-87 0.4447397298281963 1106 88-89 0.3938186395408785 1106 90-91 0.29882050189081255 1106 92-93 0.3082807027543524 1106 94-95 0.3839474502079021 1106 96-97 0.26277792635109876 1106 98-99 0.5447432203564198 1106 100-101 0.3316885487847756 1107 1 0.23264813246215255 1107 2 0.16581024165786573 1107 3 0.13803692114404953 1107 4 0.29302575754059745 1107 5 0.19274165997543236 1107 6 0.3260833405828052 1107 7 0.4375630432729736 1107 8 0.4732801543909062 1107 9 0.47709042363286613 1107 10-11 0.5689200560980581 1107 12-13 0.4476479480243043 1107 14-15 0.3962531656359687 1107 16-17 0.28208282478446733 1107 18-19 0.3081845437092028 1107 20-21 0.4268662401954231 1107 22-23 0.25192188351120137 1107 24-25 0.3272952679730352 1107 26-27 0.09394954503624575 1107 28-29 0.4422631161941837 1107 30-31 0.4777333158040875 1107 32-33 0.4065938177397399 1107 34-35 0.290177086794813 1107 36-37 0.20751787301961855 1107 38-39 0.11375447316528664 1107 40-41 0.5996770581146365 1107 42-43 0.49701735331525043 1107 44-45 0.5599859881590632 1107 46-47 0.8601881118025076 1107 48-49 0.7583186552315127 1107 50-51 0.6701183990846786 1107 52-53 0.7814176144632157 1107 54-55 0.8148940485193137 1107 56-57 0.7084150718110109 1107 58-59 0.7308864465472737 1107 60-61 0.8124711589642857 1107 62-63 0.769354005146603 1107 64-65 0.7851473756750984 1107 66-67 0.8413559240643878 1107 68-69 0.8681703584735629 1107 70-71 0.6701117399409036 1107 72-73 0.6428258453162172 1107 74-75 0.7324072279332263 1107 76-77 0.8728462951490386 1107 78-79 0.835366664668733 1107 80-81 0.9666408243245819 1107 82-83 0.6920986002002074 1107 84-85 0.7388180007779077 1107 86-87 0.4707012682897336 1107 88-89 0.3361263318485719 1107 90-91 0.7257435788138942 1107 92-93 0.6082807027543531 1107 94-95 0.7656782194386729 1107 96-97 0.8762394648126381 1107 98-99 0.8053201434333417 1107 100-101 0.6941885487847728 1108 1 -0.005558059685949246 1108 2 -0.28163629520775757 1108 3 -0.2819758461716191 1108 4 -0.4650894674833381 1108 5 -0.2819950141388361 1108 6 -0.2614206492033446 1108 7 -0.3066597463855061 1108 8 -0.28323915617404793 1108 9 -0.5141879038487858 1108 10-11 -0.44226331447327993 1108 12-13 -0.22235683971907605 1108 14-15 -0.04305420748882938 1108 16-17 0.03596089691980353 1108 18-19 -0.14223438383627496 1108 20-21 -0.3310494955211425 1108 22-23 0.015539023632484827 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-1.2200189562420292 2316 68-69 -1.278789424482067 2316 70-71 -1.2700255087025702 2316 72-73 -1.5918972635251514 2316 74-75 -1.5763423730881598 2316 76-77 -1.8188828781672726 2316 78-79 -2.1272610084879844 2316 80-81 -2.065081965333892 2316 82-83 -1.8132676621681263 2316 84-85 -1.7927771157238475 2316 86-87 -2.3858020831306312 2316 88-89 -2.177223492600838 2316 90-91 -1.848737748986938 2316 92-93 -2.331553322141911 2316 94-95 -2.3526867661980972 2316 96-97 -2.6724159772556675 2316 98-99 -2.5165558540131947 2316 100-101 -2.5290200372350142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 933.0 3 267.0 4 246.0 5 258.0 6 318.0 7 377.0 8 511.0 9 593.0 10 848.0 11 1440.0 12 2337.0 13 3207.0 14 3778.0 15 5435.0 16 7571.0 17 11212.0 18 8998.0 19 4329.0 20 3446.0 21 3339.0 22 3427.0 23 3535.0 24 3733.0 25 4150.0 26 4576.0 27 5307.0 28 6051.0 29 7042.0 30 8619.0 31 10609.0 32 13507.0 33 17915.0 34 25421.0 35 38919.0 36 56430.0 37 86623.0 38 91221.0 39 19724.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33753848464369 19.514691211019215 16.31534343856939 18.832426865767708 2 14.815680957060165 21.72651868507211 42.065119911434174 21.392680446433552 3 17.74238182578756 29.518078750630156 32.59430875996181 20.14523066362047 4 11.089726259596363 18.067338680261425 40.27940488604521 30.56353017409701 5 10.926162397656427 38.721335751306455 34.59389855243532 15.758603298601798 6 25.898557971932068 39.00601149072522 18.920206635604078 16.175223901738633 7 23.262082582195898 37.225942140956306 22.742510086702723 16.769465190145077 8 25.58955582645125 32.233916522157166 21.340332743849046 20.836194907542538 9 27.34628178989162 15.746539328254105 22.495117501877886 34.41206137997639 10-11 23.054593920559054 27.843178496386805 27.719340417357934 21.382887165696207 12-13 26.288707917195612 26.543353618426345 28.866392025608366 18.301546438769677 14-15 22.799515855932803 25.721870392744705 30.855922073407054 20.622691677915434 16-17 18.29436687871027 29.693758107207728 30.63577675231025 21.376098261771745 18-19 18.071741084935315 25.777996167686258 37.30025727570987 18.850005471668556 20-21 19.986567258658596 24.34823845476267 37.36823414169396 18.296960144884775 22-23 19.1784275004532 24.795631266351375 37.65286759823697 18.373073634958452 24-25 18.704230427676034 24.922028168530698 37.834034758062096 18.539706645731176 26-27 18.39980008494245 24.977155629153277 38.05475355964633 18.568290726257942 28-29 18.096661961982143 25.358750069712272 37.923796980707934 18.62079098759765 30-31 18.119595421411006 25.19189169768786 37.75276777699487 18.935745103906257 32-33 18.079691649042733 25.28334141246016 37.55464135111599 19.082325587381117 34-35 18.68533040479091 25.30640304065069 37.295695557424025 18.712570997134375 36-37 18.66645790078759 25.043004341186364 37.29752569536197 18.99301206266408 38-39 18.460396735091333 25.587558085539236 36.90116798450552 19.050877194863915 40-41 18.401548583592948 25.80708123093092 36.82325878183095 18.968111403645178 42-43 18.35702994803117 25.901411501776977 35.964983774485724 19.776574775706127 44-45 18.475035576140446 25.96119921887986 35.5677758987332 19.995989306246496 46-47 19.33707167514389 26.267910206874216 34.82137782453386 19.573640293448037 48-49 19.231000203751165 26.804752764045425 33.635993179697806 20.328253852505604 50-51 19.00135230637407 27.231654049787608 33.2949411301678 20.47205251367052 52-53 19.42243060008965 27.67320754878852 32.23366584664352 20.670696004478312 54-55 19.10090308632244 27.40852510914572 32.551966875038204 20.938604929493636 56-57 19.551512369446726 27.381383945138133 32.08430486347812 20.982798821937028 58-59 19.858630641181147 26.919269752512463 32.52567695246184 20.696422653844547 60-61 19.77781305235701 27.0831903282824 33.047144475142865 20.091852144217732 62-63 19.697431189511978 27.094451403665314 33.11182991282861 20.096287493994097 64-65 19.624794090394317 27.34350869968084 32.98049864442843 20.051198565496414 66-67 19.5994521505878 27.24660624020644 32.34038308702633 20.813558522179427 68-69 19.53481138248008 27.256813720758117 32.79724555137239 20.41112934538941 70-71 19.85250743790662 27.17340243203995 32.36878288605792 20.60530724399551 72-73 19.566033445734337 27.301003028820993 32.40452349695831 20.72844002848636 74-75 19.24570273870635 27.962176807123594 31.98325070950664 20.808869744663415 76-77 19.049912368579687 27.66192505958293 32.23592051604932 21.05224205578807 78-79 19.08311619489788 27.480983178520514 32.17546430437213 21.26043632220948 80-81 19.19916245552422 27.738619188650244 31.67083043264192 21.391387923183615 82-83 19.232988921022596 27.549838234915512 31.90666111716598 21.31051172689591 84-85 19.140100027779535 27.43727884969126 31.286728782709332 22.135892339819875 86-87 19.03165775515786 27.546376492923528 31.468870499777434 21.953095252141175 88-89 19.13108112891237 26.956735322541032 31.349381281969602 22.562802266577 90-91 18.813811832922 26.89763613995459 31.83172070863245 22.456831318490966 92-93 19.086178655977243 27.351782387121204 31.671280896658764 21.890758060242785 94-95 18.652275667083536 27.114084745726352 31.709930476155744 22.523709111034364 96-97 18.368807183950896 27.4733986348262 31.789368894076205 22.368425287146707 98-99 18.703628000399142 27.711665545423237 31.059408734539986 22.525297719637642 100-101 18.557390207052844 27.601895409126808 31.35075799196511 22.489956391855234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1008.0 1 725.0 2 543.5 3 658.0 4 534.5 5 472.0 6 885.0 7 2072.0 8 3653.0 9 4091.0 10 3047.5 11 2068.0 12 1926.0 13 2019.5 14 2070.0 15 2115.0 16 2136.5 17 2180.0 18 2237.0 19 2304.5 20 2466.5 21 2685.0 22 2895.5 23 3169.5 24 3489.0 25 3946.5 26 4563.0 27 5173.5 28 5741.5 29 6443.5 30 7507.5 31 8568.5 32 9582.5 33 10743.5 34 11755.5 35 12119.0 36 13160.0 37 14614.0 38 15108.5 39 15181.5 40 15098.5 41 14888.0 42 14448.5 43 14599.5 44 15018.5 45 15391.0 46 15419.5 47 16077.0 48 18487.5 49 17793.5 50 14086.0 51 11899.5 52 10817.0 53 10294.5 54 9735.5 55 8774.5 56 7811.5 57 6784.5 58 6227.0 59 5812.5 60 5133.5 61 4559.5 62 3749.5 63 2576.0 64 1631.0 65 1168.0 66 816.0 67 565.0 68 410.0 69 288.5 70 198.0 71 137.0 72 116.0 73 93.0 74 65.0 75 44.5 76 32.0 77 17.5 78 9.0 79 6.0 80 4.5 81 5.0 82 4.5 83 1.0 84 0.5 85 1.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03474478667344404 2 0.034959260665255426 3 0.021876347164761067 4 0.04182242840321968 5 0.06477114552703767 6 0.06777378141239702 7 0.06369877556798074 8 0.06713035943696287 9 0.06584351548609457 10-11 0.06991852133051085 12-13 0.06766654441649134 14-15 0.06005271770718724 16-17 0.050723099063392084 18-19 0.04718427819850426 20-21 0.04965072910433516 22-23 0.027130959964139948 24-25 0.013511861484117128 26-27 0.012653965516871597 28-29 0.011796069549626064 30-31 0.009329618643795161 32-33 0.007506589713398405 34-35 0.008793433664266704 36-37 0.0053618497952845744 38-39 0.005683560783001649 40-41 0.005254612799378883 42-43 0.0034315838689821278 44-45 7.506589713398405E-4 46-47 0.0016085549385853725 48-49 4.2894798362276597E-4 50-51 0.003538820864887819 52-53 0.00214473991811383 54-55 0.0048256648157561175 56-57 0.014584231443174044 58-59 0.006755930742058564 60-61 0.01565660140223096 62-63 0.010509225598757766 64-65 0.007077641729775638 66-67 0.0151204164227025 68-69 0.021876347164761067 70-71 0.013726335475928511 72-73 0.014798705434985427 74-75 0.018873711279401705 76-77 0.021447399181138298 78-79 0.033565179718481435 80-81 0.028417803915008248 82-83 0.03195662477989607 84-85 0.01855200029168463 86-87 0.02134016218523261 88-89 0.019624370250741544 90-91 0.019624370250741544 92-93 0.027130959964139948 94-95 0.018444763295778936 96-97 0.03485202366934974 98-99 0.05501257889961974 100-101 0.06198298363348968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 466257.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.64185896037122 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92168779559682 43.91349421237049 2 12.042990533032622 13.40188761400174 3 3.8309703073041734 6.394869285611665 4 1.6162332834492426 3.5972089761896178 5 0.9246660522710882 2.5725069032955568 6 0.5729974172783241 1.9129584886114492 7 0.38008696568766126 1.4804121736227813 8 0.2622485061618257 1.1673595513939479 9 0.21626456219335385 1.0830026040920122 >10 1.1287880456693975 11.520776796848907 >50 0.06446483423544344 2.3980970648575415 >100 0.03242542558084514 3.4436802704723872 >500 0.0023161018272032236 0.8269300219122242 >1k 0.0030881357696042984 2.842491405191266 >5k 7.720339424010746E-4 3.4443246315284224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9505 2.0385752921671956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6531 1.4007296405201424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4597 0.9859369403569277 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1538 0.329860999405907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1412 0.3028372764376728 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1303 0.27945961133023206 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1196 0.25651089420641404 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1084 0.23248980712353914 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1060 0.22734243132006599 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1044 0.22391084745108383 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.15699496200593235 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 731 0.15678048801412095 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 632 0.13554756282479405 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 610 0.13082913500494364 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 607 0.1301857130295095 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 538 0.11538700759452405 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.1447399181138299E-4 0.0 5 0.0 0.0 0.0 2.1447399181138299E-4 0.0 6 0.0 0.0 0.0 2.1447399181138299E-4 0.0 7 0.0 0.0 0.0 2.1447399181138299E-4 0.0 8 0.0 0.0 0.0 2.1447399181138299E-4 0.0 9 0.0 0.0 0.0 2.1447399181138299E-4 0.0 10-11 0.0 0.0 0.0 3.217109877170745E-4 0.0 12-13 0.0 0.0 0.0 4.2894798362276597E-4 0.0 14-15 0.0 0.0 0.0 4.2894798362276597E-4 0.0 16-17 0.0 0.0 0.0 6.43421975434149E-4 0.0 18-19 0.0 0.0 0.0 6.43421975434149E-4 0.0 20-21 0.0 0.0 0.0 0.001072369959056915 0.0 22-23 0.0 0.0 0.0 0.0017157919344910639 0.0 24-25 0.0 0.0 0.0 0.0028953988894536704 0.0 26-27 0.0 0.0 0.0 0.004932901811661809 0.0 28-29 0.0 0.0 0.0 0.008257248684738245 0.0 30-31 0.0 0.0 0.0 0.013833572471834202 0.0 32-33 0.0 0.0 0.0 0.01962437025074154 0.0 34-35 0.0 0.0 0.0 0.026809248976422874 0.0 36-37 0.0 0.0 0.0 0.03399412770210421 0.0 38-39 0.0 0.0 0.0 0.04010663646872862 0.0 40-41 0.0 0.0 0.0 0.04965072910433516 0.0 42-43 0.0 0.0 0.0 0.07217049824453038 0.0 44-45 0.0 0.0 0.0 0.10959620981561671 0.0 46-47 0.0 0.0 0.0 0.1450916554604006 0.0 48-49 0.0 0.0 0.0 0.21833452366398787 0.0 50-51 0.0 0.0 0.0 0.28718067503544187 0.0 52-53 0.0 0.0 0.0 0.3577426183413868 0.0 54-55 0.0 0.0 0.0 0.48589082844868814 0.0 56-57 0.0 0.0 0.0 0.6085699517647992 0.0 58-59 0.0 0.0 0.0 0.7687820236479024 0.0 60-61 0.0 0.0 0.0 0.943363852982368 0.0 62-63 0.0 0.0 0.0 1.1911885505204212 0.0 64-65 0.0 0.0 0.0 1.3644835359040186 0.0 66-67 0.0 0.0 0.0 1.6185279792046017 0.0 68-69 0.0 0.0 0.0 1.943027128815224 0.0 70-71 0.0 0.0 0.0 2.1361609584413745 0.0 72-73 0.0 0.0 0.0 2.4602311600683744 0.0 74-75 0.0 0.0 0.0 2.827088923061745 0.0 76-77 0.0 0.0 0.0 3.0892833780511606 0.0 78-79 0.0 0.0 0.0 3.329065300896287 0.0 80-81 0.0 0.0 0.0 3.6986039887872995 0.0 82-83 0.0 0.0 0.0 4.1557553023332625 0.0 84-85 0.0 0.0 0.0 4.762931173151288 0.0 86-87 0.0 0.0 0.0 5.522061867167677 0.0 88-89 0.0 0.0 0.0 6.333202504198328 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3645 0.0 40.174114 1 GTATCAA 6285 0.0 39.865612 1 TATCAAC 7935 0.0 31.459524 2 ATCAACG 7940 0.0 31.319942 3 TCAACGC 7980 0.0 31.222536 4 CAACGCA 8075 0.0 30.802946 5 AACGCAG 8155 0.0 30.552523 6 ACGCAGA 9065 0.0 27.22321 7 CGCAGAG 9075 0.0 27.140816 8 GTACATG 3730 0.0 27.022167 1 GTCGGGA 160 6.163282E-8 26.746214 2 GCAGAGT 9215 0.0 26.057686 9 TACATGG 3860 0.0 25.745348 2 GAGTACT 5380 0.0 25.495098 12-13 ACATGGG 3810 0.0 25.334414 3 AGTACTT 5500 0.0 24.333738 12-13 CATGGGG 985 0.0 24.13646 4 AGAGTAC 7500 0.0 24.121807 10-11 TAAGGTA 100 0.0016423227 23.774412 4 CGATTCG 50 0.0016590293 23.736164 38-39 >>END_MODULE