FastQCFastQC Report
Thu 26 May 2016
SRR937086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466257
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59881.2842702629665614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47711.0232554149321083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33430.7169865546254534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28130.6033153389654203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18180.38991371711309425No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.26551880186249216No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.22627006136100905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.18466210694960075No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7870.1687910315555584No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7480.16042654587491448No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6220.1334028229066802No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5340.11452911162727851No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.10895278784018256No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT500.001614615738.001031
ATTCGTG350.00839405627.14067860-61
GAGTACT42000.024.42530312-13
GTACATG47250.024.0271071
GTATCAA81150.023.9994181
GGTATCA61500.023.2485981
TACATGG48700.023.1141642
AGTACTT43050.023.0573112-13
ACATGGG47700.023.00133
GTACTTT44450.022.91998714-15
GCCTAGA1252.2526973E-422.8006171
GTATAGG2103.43407E-822.6196611
AGAGTAC70850.021.88545410-11
CATGGGA35150.021.7550134
GACTATC550.00287293221.59612734-35
TGTACTA1100.00287616721.5891785
TACTTTT47450.020.8202514-15
GGGATAG1404.8519205E-420.3533299
ACTTTTT50500.020.26817716-17
GTGTGCA2851.9590516E-919.9962529
Produced by FastQC (version 0.11.5)