##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 466257 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21031105163033 33.0 31.0 34.0 31.0 34.0 2 32.24953619999271 33.0 31.0 34.0 30.0 34.0 3 32.3564064453724 34.0 31.0 34.0 30.0 34.0 4 36.06424782898702 37.0 35.0 37.0 35.0 37.0 5 36.00259084582108 37.0 35.0 37.0 35.0 37.0 6 36.066960924983434 37.0 35.0 37.0 35.0 37.0 7 36.02177983386844 37.0 35.0 37.0 35.0 37.0 8 36.02020773950847 37.0 35.0 37.0 35.0 37.0 9 37.68228466275037 39.0 37.0 39.0 35.0 39.0 10-11 37.59605324960269 39.0 37.0 39.0 35.0 39.0 12-13 37.520627036162466 39.0 37.0 39.0 35.0 39.0 14-15 38.63386930383887 40.0 38.0 41.0 34.0 41.0 16-17 38.601728231426016 40.0 38.0 41.0 34.5 41.0 18-19 38.72394730802969 40.0 38.0 41.0 34.5 41.0 20-21 38.83240358857883 40.0 38.0 41.0 35.0 41.0 22-23 38.751492202798026 40.0 38.0 41.0 35.0 41.0 24-25 38.64640852577013 40.0 38.0 41.0 34.0 41.0 26-27 38.446290350600634 40.0 38.0 41.0 34.0 41.0 28-29 38.26940936007395 40.0 38.0 41.0 34.0 41.0 30-31 38.07098338469986 40.0 37.0 41.0 34.0 41.0 32-33 37.84323452516531 40.0 36.5 41.0 33.5 41.0 34-35 37.64312707369541 40.0 36.0 41.0 33.0 41.0 36-37 37.41079061547602 40.0 35.5 41.0 33.0 41.0 38-39 37.204001226791235 40.0 35.0 41.0 33.0 41.0 40-41 36.91058686518379 40.0 35.0 41.0 32.0 41.0 42-43 36.56421565788825 39.0 35.0 41.0 31.0 41.0 44-45 36.09159540768289 39.0 35.0 41.0 30.0 41.0 46-47 35.44433113068544 39.0 35.0 41.0 27.0 41.0 48-49 34.84088389021505 39.0 35.0 41.0 23.5 41.0 50-51 34.50408144006417 39.0 35.0 40.0 22.0 41.0 52-53 34.27735883858044 38.5 34.5 40.0 20.0 41.0 54-55 33.985281722311946 38.0 34.0 40.0 18.5 41.0 56-57 33.71784874007253 38.0 34.0 40.0 16.5 41.0 58-59 33.373161153612706 38.0 33.5 40.0 11.5 41.0 60-61 33.00766958994717 37.0 33.0 40.0 8.0 41.0 62-63 32.69013226611075 37.0 33.0 40.0 6.5 41.0 64-65 32.27836579397199 36.0 32.5 39.0 2.0 41.0 66-67 31.828383273602327 36.0 32.0 39.0 2.0 40.0 68-69 31.38921345952983 35.0 31.0 38.5 2.0 40.0 70-71 30.977996040810112 35.0 31.0 38.0 2.0 40.0 72-73 30.626052799207304 35.0 31.0 37.0 2.0 39.5 74-75 30.167384082169278 35.0 30.0 37.0 2.0 39.0 76-77 28.726299015349902 33.5 28.0 35.5 2.0 37.5 78-79 29.38107202680067 34.0 29.0 36.0 2.0 38.0 80-81 29.035982301606197 34.0 29.0 35.5 2.0 37.0 82-83 28.862894283624698 34.0 29.0 35.0 2.0 37.0 84-85 28.54272000205895 34.0 29.0 35.0 2.0 36.5 86-87 28.19183733434565 34.0 29.0 35.0 2.0 36.0 88-89 27.823955458041382 34.0 28.5 35.0 2.0 36.0 90-91 27.386711620415348 34.0 27.0 35.0 2.0 35.0 92-93 27.006489982992214 33.0 26.0 35.0 2.0 35.0 94-95 26.56434541465329 33.0 25.0 35.0 2.0 35.0 96-97 25.985995920704674 33.0 24.5 35.0 2.0 35.0 98-99 25.359169942756893 33.0 21.0 35.0 2.0 35.0 100-101 22.519051724692606 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18068153717494084 1101 2 0.1537500940860852 1101 3 0.10349375673137473 1101 4 0.014653544862035517 1101 5 0.18015422933410008 1101 6 0.1298151051185812 1101 7 9.319280572057664E-4 1101 8 0.05296523008753695 1101 9 0.10875564808193872 1101 10-11 0.20019308350866538 1101 12-13 0.12765905017396761 1101 14-15 0.09309057359602235 1101 16-17 -0.008622573664588629 1101 18-19 0.17746703790049878 1101 20-21 0.1842619117786839 1101 22-23 0.04016596139452844 1101 24-25 0.23019655004549833 1101 26-27 0.07209266162860928 1101 28-29 -0.04138041929889624 1101 30-31 -0.028523832881987232 1101 32-33 0.09737862682411702 1101 34-35 0.2071293441486688 1101 36-37 0.2297425786513685 1101 38-39 -0.05088327705743012 1101 40-41 0.11071922240238763 1101 42-43 0.23718017017768744 1101 44-45 0.06570724641179027 1101 46-47 -0.11398396040938508 1101 48-49 0.11747013722357735 1101 50-51 0.09210671417186944 1101 52-53 0.20204458599175013 1101 54-55 0.2816674153221683 1101 56-57 0.3873042803451199 1101 58-59 0.4276660664911702 1101 60-61 0.4821306099121756 1101 62-63 0.4662863368777508 1101 64-65 0.8044877732851248 1101 66-67 0.4709398898230184 1101 68-69 0.2069492375266293 1101 70-71 0.4120330123505518 1101 72-73 0.7543985945150773 1101 74-75 0.477662329548167 1101 76-77 0.01904858605125881 1101 78-79 0.34535062639386993 1101 80-81 0.6626693706471336 1101 82-83 0.5743527199864502 1101 84-85 0.4553991442053409 1101 86-87 0.4287384690187963 1101 88-89 0.5312652588837103 1101 90-91 0.28600920658771756 1101 92-93 0.2707361206237415 1101 94-95 0.18799173036909522 1101 96-97 -0.029759074045898615 1101 98-99 0.12014067461180034 1101 100-101 0.07315718757988776 1102 1 0.2698738237861349 1102 2 0.3600519889125664 1102 3 0.24273156810033214 1102 4 0.2010944147699476 1102 5 0.21519017350851755 1102 6 0.2547816976470827 1102 7 0.26545794964635405 1102 8 0.24241616980007308 1102 9 0.33498878013998024 1102 10-11 0.49351634552743207 1102 12-13 0.3783917496335576 1102 14-15 0.47134695272439586 1102 16-17 0.461105727333333 1102 18-19 0.4520991027313741 1102 20-21 0.3007679205937208 1102 22-23 0.447862816335892 1102 24-25 0.6069410995315891 1102 26-27 0.5443395462403444 1102 28-29 0.15159812291229713 1102 30-31 0.6105044740047063 1102 32-33 0.5754282193662092 1102 34-35 0.33662802823403126 1102 36-37 0.2504914495920758 1102 38-39 0.188510133856731 1102 40-41 0.5950358301058145 1102 42-43 0.45449520603126103 1102 44-45 0.5957954874348061 1102 46-47 0.48336427907349844 1102 48-49 0.2793129160004355 1102 50-51 0.17426757737563037 1102 52-53 0.21049497759526759 1102 54-55 0.20325158629594142 1102 56-57 0.3455607953682929 1102 58-59 0.3535943412161018 1102 60-61 0.416999629974768 1102 62-63 0.3168077199244195 1102 64-65 0.3644003929291628 1102 66-67 0.42186488042363024 1102 68-69 0.40192883065759943 1102 70-71 0.3429089902268778 1102 72-73 0.1696410081879023 1102 74-75 0.4071906839980457 1102 76-77 0.2663114383019014 1102 78-79 0.19507362749461876 1102 80-81 0.21788342760517665 1102 82-83 0.23169851336293945 1102 84-85 0.07260954106345352 1102 86-87 0.07897922930489898 1102 88-89 -0.18324581427752307 1102 90-91 0.02151829115505066 1102 92-93 0.17590080247136797 1102 94-95 0.04161717712324631 1102 96-97 -0.10042097231505309 1102 98-99 0.1003849681626896 1102 100-101 0.7824306486845316 1103 1 0.18182704170605035 1103 2 0.18303335537219567 1103 3 0.1480110712059215 1103 4 0.18609507553379245 1103 5 0.0780816760854961 1103 6 0.2301682444952391 1103 7 0.19211316307308124 1103 8 0.12030701161321389 1103 9 0.13900622430543308 1103 10-11 0.18665761683706705 1103 12-13 0.056351971650208554 1103 14-15 0.25298102170813763 1103 16-17 0.24635747835751687 1103 18-19 0.3898551487205353 1103 20-21 0.29962810296454023 1103 22-23 0.5250730713907359 1103 24-25 0.4097526496835613 1103 26-27 0.29019325312550137 1103 28-29 0.37888436580908547 1103 30-31 0.40541989623280017 1103 32-33 0.39629514152822054 1103 34-35 0.5560511813990843 1103 36-37 0.3949509447685031 1103 38-39 0.10153709302152691 1103 40-41 0.13530938633681444 1103 42-43 0.015467189644319035 1103 44-45 0.4060736690127058 1103 46-47 0.5597485793245838 1103 48-49 0.5808359766585554 1103 50-51 0.5162789425137362 1103 52-53 0.2782067524069518 1103 54-55 0.08289345229304246 1103 56-57 0.2120039036014134 1103 58-59 0.3356380837825057 1103 60-61 0.2673531386307815 1103 62-63 0.26034544953984806 1103 64-65 0.20951734812937417 1103 66-67 0.4206094291219209 1103 68-69 0.5354857224244789 1103 70-71 0.428808289817205 1103 72-73 0.5731034107252384 1103 74-75 0.2359041767957173 1103 76-77 0.31340738121190626 1103 78-79 0.44684117077465046 1103 80-81 0.8618969071875711 1103 82-83 0.5020170015352647 1103 84-85 0.6730126070076885 1103 86-87 0.8421614679727973 1103 88-89 0.8790311779254658 1103 90-91 0.7634404531743435 1103 92-93 0.622940580454717 1103 94-95 1.039700961978543 1103 96-97 1.2114760806782066 1103 98-99 1.2704655813515338 1103 100-101 1.121765920258472 1104 1 0.22529762813559273 1104 2 0.2532033186372118 1104 3 0.15400480886286516 1104 4 0.14535914789864535 1104 5 0.2544710055440902 1104 6 0.21872565442289726 1104 7 0.34571963825393937 1104 8 0.25600134378343853 1104 9 0.3271220981171723 1104 10-11 0.2945321039286597 1104 12-13 0.27806526480357974 1104 14-15 0.31842736713979747 1104 16-17 0.5364538693241911 1104 18-19 0.3820425687784521 1104 20-21 0.38826660614176234 1104 22-23 0.280810994380289 1104 24-25 0.26009979018721197 1104 26-27 0.30308503328860326 1104 28-29 0.26868324431757884 1104 30-31 0.34528937958950223 1104 32-33 0.30313511410489014 1104 34-35 0.47280525141044905 1104 36-37 0.5883506705351778 1104 38-39 0.627843794342553 1104 40-41 0.5685878047882156 1104 42-43 0.7688708037939378 1104 44-45 0.6610786829801896 1104 46-47 0.5095782067561032 1104 48-49 0.8036978776685189 1104 50-51 0.8388382147721671 1104 52-53 0.7084269065097075 1104 54-55 0.7426676191046653 1104 56-57 0.7655957973462222 1104 58-59 0.721062531478637 1104 60-61 0.41017574650782507 1104 62-63 0.534041562180164 1104 64-65 0.4389498488712391 1104 66-67 0.5596933056350686 1104 68-69 0.4507688580983462 1104 70-71 0.7535675682151926 1104 72-73 0.6116649136875729 1104 74-75 0.2257675206003995 1104 76-77 0.5986624795982287 1104 78-79 0.6253575991925864 1104 80-81 0.5473702154851772 1104 82-83 0.49744815157568567 1104 84-85 0.4401240501093895 1104 86-87 0.5329633957921338 1104 88-89 0.20836698072305282 1104 90-91 0.21729276625056926 1104 92-93 0.3504212152843955 1104 94-95 0.35652231124742073 1104 96-97 0.6739902783922496 1104 98-99 0.963132398096139 1104 100-101 1.0370938234991343 1105 1 0.3129586616608364 1105 2 0.2303195305582051 1105 3 0.204871901265399 1105 4 0.2840473613797627 1105 5 0.262463740479852 1105 6 0.17574360169296455 1105 7 0.3021146994073831 1105 8 0.2213758613377692 1105 9 0.18940896337002755 1105 10-11 0.17337929890365444 1105 12-13 0.32283738744811075 1105 14-15 0.3516164276296152 1105 16-17 0.3009083200492384 1105 18-19 0.4575471356397429 1105 20-21 0.4161727773714077 1105 22-23 0.26519663656423376 1105 24-25 0.4192806658370216 1105 26-27 0.43585146263760777 1105 28-29 0.45248849635360244 1105 30-31 0.5719064094123567 1105 32-33 0.26671082969625814 1105 34-35 0.36254713206081135 1105 36-37 0.46969086531807136 1105 38-39 0.5626925261580666 1105 40-41 0.632659209658371 1105 42-43 0.4804250501890621 1105 44-45 0.4755735921959925 1105 46-47 0.4164305361755396 1105 48-49 0.2992066941266742 1105 50-51 0.3421738572471753 1105 52-53 0.2690244114724223 1105 54-55 0.11276966764321372 1105 56-57 -0.04413869016985217 1105 58-59 0.18791130986506488 1105 60-61 0.06790128076364965 1105 62-63 0.3341882605926756 1105 64-65 0.34401792974164636 1105 66-67 0.2162385580203683 1105 68-69 0.18814522684693102 1105 70-71 0.4162761914040196 1105 72-73 0.6168066348325176 1105 74-75 0.2180964161127079 1105 76-77 0.3013114920035598 1105 78-79 0.4240897057701467 1105 80-81 0.9052270215347384 1105 82-83 0.8793297333571388 1105 84-85 0.5488438736055841 1105 86-87 0.6281994812730645 1105 88-89 0.8379287483225006 1105 90-91 0.7740932855163756 1105 92-93 0.8006121560752852 1105 94-95 0.2535641843300098 1105 96-97 0.5680894511762027 1105 98-99 0.7726383970133632 1105 100-101 0.9998632799582268 1106 1 0.014256568700794503 1106 2 0.10400803286861304 1106 3 0.11937991974868822 1106 4 -0.04904587047672493 1106 5 -0.014357406570979947 1106 6 0.04496757559891762 1106 7 0.011845604544653554 1106 8 0.09460228156065398 1106 9 0.2463401946828796 1106 10-11 0.06602335978976726 1106 12-13 0.010765492911552599 1106 14-15 0.1167292300215621 1106 16-17 0.18128342197910996 1106 18-19 0.21339791030678157 1106 20-21 0.021880850217293357 1106 22-23 0.12847130359331516 1106 24-25 -0.05425833932742563 1106 26-27 -0.18374965179566516 1106 28-29 -0.12252509429603009 1106 30-31 -0.1087642891470395 1106 32-33 -0.11082382646031164 1106 34-35 -0.056150204171864004 1106 36-37 0.14587664949855395 1106 38-39 0.03405023205640845 1106 40-41 0.21319400172143332 1106 42-43 0.230003740050428 1106 44-45 0.01104858540067255 1106 46-47 0.10100514884202028 1106 48-49 0.2651587191334741 1106 50-51 0.1133107150889856 1106 52-53 0.24932476482931065 1106 54-55 0.4891762998802349 1106 56-57 0.32035913532620697 1106 58-59 0.4758733919525966 1106 60-61 0.38068328674353324 1106 62-63 0.44085787190009995 1106 64-65 0.5306413328403181 1106 66-67 0.4193134424998455 1106 68-69 0.21586675443594672 1106 70-71 0.5578873629180201 1106 72-73 0.4713595024595918 1106 74-75 0.40230408123910166 1106 76-77 0.012398064441722312 1106 78-79 0.21706333990985982 1106 80-81 0.253565564617098 1106 82-83 0.3467733321611597 1106 84-85 0.1333267493870487 1106 86-87 0.041552294537538614 1106 88-89 -0.09386657649402608 1106 90-91 -0.03725830458963131 1106 92-93 0.25451607018237965 1106 94-95 0.2862191177358291 1106 96-97 0.6266277768081672 1106 98-99 0.8364295087285036 1106 100-101 1.1932004639756215 1107 1 0.12194887639309826 1107 2 0.13093110979168898 1107 3 0.12899530436406792 1107 4 0.20287720644635243 1107 5 0.2433349011213295 1107 6 0.06612142175276858 1107 7 -0.04200054930150543 1107 8 0.16960228156065682 1107 9 0.02710942545211026 1107 10-11 0.2131387444051498 1107 12-13 0.17999626214232478 1107 14-15 0.3090369223292626 1107 16-17 0.3437834219791185 1107 18-19 0.33935944876832025 1107 20-21 0.17091931175575326 1107 22-23 0.21212514974715901 1107 24-25 0.277472429903348 1107 26-27 0.2797118866658721 1107 28-29 0.2774749057039756 1107 30-31 0.23065878777604354 1107 32-33 0.4680223273858459 1107 34-35 0.38423441121275914 1107 36-37 0.562222803344703 1107 38-39 0.27443484744102165 1107 40-41 0.5583863094137342 1107 42-43 0.47327297081966435 1107 44-45 0.37258704693913103 1107 46-47 1.0356205334574042 1107 48-49 1.3122741037488552 1107 50-51 1.2411953304736016 1107 52-53 1.0185555340600772 1107 54-55 1.2160993768033066 1107 56-57 1.2059360584031253 1107 58-59 1.3268349304141296 1107 60-61 1.1681832867435418 1107 62-63 1.4687424872847146 1107 64-65 1.2748721020710931 1107 66-67 1.2606595963460059 1107 68-69 1.402405215974408 1107 70-71 1.0598104398410975 1107 72-73 1.0905902716903597 1107 74-75 1.0599963889314097 1107 76-77 1.2508596029032581 1107 78-79 1.1526402629867825 1107 80-81 1.4045271030786388 1107 82-83 1.269850255238083 1107 84-85 1.2996729032332013 1107 86-87 1.1588599868452327 1107 88-89 1.2753641927367418 1107 90-91 0.7877416954103715 1107 92-93 1.139131454797763 1107 94-95 1.0919883485050583 1107 96-97 0.8391277768081657 1107 98-99 1.2046987394977364 1107 100-101 1.2162773870525463 1108 1 0.07106314386836488 1108 2 -0.030878657204802096 1108 3 0.038522913684211346 1108 4 0.0973473628459729 1108 5 0.020106366170800527 1108 6 0.10158264103141335 1108 7 -0.15291660420096065 1108 8 0.1288506058504737 1108 9 0.21118219915144465 1108 10-11 0.1752597147211432 1108 12-13 0.21696961037086027 1108 14-15 0.08331078125041813 1108 16-17 -0.1622531244619978 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-1.463921304297596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 5.0 5 12.0 6 34.0 7 83.0 8 176.0 9 372.0 10 675.0 11 1084.0 12 1713.0 13 2473.0 14 3171.0 15 4628.0 16 6579.0 17 9352.0 18 8299.0 19 4201.0 20 3325.0 21 3311.0 22 3092.0 23 3235.0 24 3783.0 25 4160.0 26 4899.0 27 5693.0 28 6941.0 29 8397.0 30 10342.0 31 12561.0 32 15931.0 33 21183.0 34 29232.0 35 42846.0 36 63846.0 37 96633.0 38 77399.0 39 6589.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58638761426067 21.122216287067012 15.889983674747773 18.40141242392454 2 15.347277929587797 23.594565725635206 39.906826303123374 21.15133004165362 3 19.09286682567737 29.661358683528665 31.452159514044993 19.793614976748973 4 12.134295034712615 18.9584285061672 38.783117465260574 30.124158993859613 5 11.632710402649296 40.90559093336651 32.89635957678176 14.56533908720243 6 27.088923061744918 39.39351044595577 17.878766431388698 15.638800060910613 7 25.173241366885645 37.17005857284717 21.5454137953961 16.11128626487109 8 26.94179390336231 33.38502156535988 20.25449483868339 19.418689692594427 9 28.18595752986014 16.808326738258085 21.326221375764867 33.679494356116905 10-11 24.21829591834546 28.916348708973803 26.66287047701161 20.202484895669127 12-13 26.972785395179056 27.21203113304465 27.68891405383726 18.126269417939035 14-15 23.25219782223109 27.30918785133521 28.89264933287865 20.545964993555057 16-17 19.393509795573895 30.732118479849614 28.862010502802644 21.01236122177385 18-19 19.414959216370935 27.847829770440608 34.10950559903569 18.627705414152768 20-21 21.592156963675986 26.16997820902181 34.057411506322815 18.180453320979392 22-23 20.761725829317307 27.034017719841202 33.9902671702516 18.21398928058989 24-25 20.237417301284463 27.23199396000433 34.30239229728962 18.228196441421588 26-27 20.03751383443294 27.07720257041619 34.4687577751658 18.416525819985072 28-29 19.364615316968436 27.64941127768668 34.39234355343973 18.593629851905156 30-31 19.72644706200369 27.210161211948286 34.18103064518032 18.882361080867707 32-33 19.509430482097738 27.75171902010492 33.87692116314284 18.8619293346545 34-35 20.02355030499575 27.35256393757668 34.079200233358215 18.544685524069354 36-37 20.109119418944992 27.2673061103228 33.80707077532275 18.816503695409455 38-39 19.690123836784686 27.897863340901313 33.62189617100137 18.790116651312637 40-41 19.84258487686594 27.92558011837772 33.368080914676455 18.863754090079887 42-43 19.720729706251408 28.378216059114358 32.56930815181837 19.33174608281587 44-45 19.972374831498268 28.235045571876377 32.197477058562995 19.595102538062356 46-47 20.625359253952865 28.897851768601846 31.306783572549996 19.170005404895292 48-49 20.60892290501116 29.34019232192206 30.26630591560956 19.78457885745722 50-51 20.0732885923314 29.807566505590483 30.38999311511907 19.729151786959047 52-53 20.786634337073544 30.077460189451987 29.488706984285972 19.647198489188497 54-55 20.081845030638267 29.83493727573582 29.70142435232827 20.381793341297637 56-57 20.484952343367965 29.9682360611858 29.567701586266654 19.979110009179585 58-59 20.8227345844504 29.372868632707778 29.757426273458442 20.046970509383378 60-61 20.12023411980946 29.914081718880357 30.351179714191957 19.614504447118218 62-63 20.156265750433775 29.649956997964644 30.543455647850866 19.65032160375072 64-65 20.063726373409523 30.14680016816384 30.059500827949513 19.72997263047712 66-67 20.085469168900804 29.873565683646113 29.547667560321717 20.49329758713137 68-69 20.109254574525927 29.98184452349113 29.53594687834115 20.3729540236418 70-71 20.455478948898033 30.055583617256982 29.32901790837014 20.15991952547484 72-73 20.235096017203194 30.051963512926527 29.321578532473175 20.391361937397104 74-75 20.049096940181737 30.99477607698503 28.823807618927095 20.132319363906134 76-77 20.102266353661847 30.21222198748052 29.065800720025482 20.61971093883215 78-79 20.01340633529781 30.43999592447407 28.637969959083865 20.908627781144258 80-81 20.264060661888898 30.820160147901422 28.328184164724778 20.5875950254849 82-83 20.161402756179957 30.37256689366722 28.759182798005256 20.70684755214757 84-85 19.761117330896894 30.761130199419203 28.26645991240826 21.211292557275648 86-87 19.73321580158582 30.60361560255396 28.048372464113143 21.614796131747084 88-89 19.870263291095803 30.258529722124518 28.046957439324654 21.824249547455025 90-91 19.846992613436512 30.02485780221854 28.25499944236164 21.873150141983306 92-93 19.92778645207783 30.70223225009437 27.936476956864897 21.433504340962905 94-95 19.77754590582162 30.64030618536912 27.727620275236543 21.854527633572722 96-97 19.647939432284907 30.78808806339878 27.759166121542933 21.804806382773375 98-99 19.54327567532788 31.338752399438075 27.04056792956644 22.07740399566761 100-101 19.022372069979472 31.450227452595275 26.960049415443617 22.567351061981636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1264.0 1 868.5 2 485.0 3 527.0 4 542.5 5 611.0 6 904.0 7 1505.5 8 2146.0 9 2446.5 10 2229.5 11 1931.5 12 1982.0 13 2045.0 14 2019.0 15 2064.5 16 2127.0 17 2171.5 18 2257.0 19 2296.5 20 2337.0 21 2513.0 22 2762.0 23 3104.5 24 3367.5 25 3721.5 26 4263.5 27 4937.0 28 5684.0 29 6591.5 30 7574.0 31 8269.5 32 9254.0 33 10564.0 34 11767.5 35 12211.0 36 13205.5 37 14698.0 38 15260.0 39 15316.5 40 15002.5 41 14894.0 42 14651.0 43 14667.5 44 15297.0 45 15510.5 46 15249.5 47 15862.0 48 18410.5 49 17745.5 50 14066.0 51 12098.0 52 10922.0 53 10427.0 54 9848.5 55 8879.5 56 8063.0 57 7326.0 58 6912.5 59 6560.5 60 6004.0 61 5263.0 62 4329.0 63 3000.0 64 1792.5 65 1212.0 66 838.5 67 628.5 68 472.5 69 303.0 70 212.0 71 176.0 72 132.0 73 98.0 74 73.5 75 61.0 76 42.0 77 14.5 78 6.5 79 4.0 80 2.0 81 2.0 82 1.5 83 1.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023163191115629363 2 0.006648693746152874 3 0.008793433664266704 4 0.0 5 0.00428947983622766 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0723699590569149E-4 18-19 0.0034315838689821278 20-21 0.001930265926302447 22-23 0.0 24-25 0.006755930742058564 26-27 0.006219745762530107 28-29 0.007613826709304096 30-31 0.028203329923196862 32-33 6.43421975434149E-4 34-35 0.0045039538280390425 36-37 0.0151204164227025 38-39 0.008257248684738245 40-41 0.008042774692926863 42-43 0.009007907656078085 44-45 0.003324346873076437 46-47 0.002788161893547979 48-49 0.0016085549385853725 50-51 0.003860531852604894 52-53 0.0037532948566992024 54-55 0.00214473991811383 56-57 0.001072369959056915 58-59 0.001501317942679681 60-61 0.0 62-63 0.0 64-65 0.009651329631512235 66-67 0.001501317942679681 68-69 0.0013940809467739895 70-71 0.0048256648157561175 72-73 0.013833572471834204 74-75 0.00782830070111548 76-77 0.0050401388075675 78-79 0.012761202512777289 80-81 1.0723699590569149E-4 82-83 0.0068631677379642556 84-85 6.43421975434149E-4 86-87 0.0 88-89 0.001072369959056915 90-91 0.001072369959056915 92-93 2.1447399181138299E-4 94-95 7.506589713398405E-4 96-97 4.2894798362276597E-4 98-99 4.2894798362276597E-4 100-101 0.001286843950868298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 466257.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.50654248248974 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59398715913991 42.29379957475424 2 12.536699179810235 13.666642528690417 3 4.11730052250953 6.732594475300288 4 1.8255709696714564 3.980222464527889 5 0.9999493459873375 2.7251890753698325 6 0.6382306020762943 2.0872646055417925 7 0.4257964493452384 1.6246084578590674 8 0.3004384737116725 1.3100689944591721 9 0.21936373615940177 1.0761082923681091 >10 1.2172996883026876 12.18363059677345 >50 0.08278746350076419 3.150340597279805 >100 0.037451471570521165 3.7790764661063165 >500 0.00236535609919081 0.8724086229824102 >1k 0.00236535609919081 3.231349968573764 >5k 3.9422601653180166E-4 1.286695279413466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5988 1.2842702629665614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4771 1.0232554149321083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3343 0.7169865546254534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2813 0.6033153389654203 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1818 0.38991371711309425 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1238 0.26551880186249216 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.22627006136100905 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 861 0.18466210694960075 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 787 0.1687910315555584 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 748 0.16042654587491448 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 622 0.1334028229066802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 534 0.11452911162727851 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 508 0.10895278784018256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0723699590569149E-4 0.0 12-13 0.0 0.0 0.0 2.1447399181138299E-4 0.0 14-15 0.0 0.0 0.0 2.1447399181138299E-4 0.0 16-17 0.0 0.0 0.0 4.2894798362276597E-4 0.0 18-19 0.0 0.0 0.0 4.2894798362276597E-4 0.0 20-21 0.0 0.0 0.0 8.578959672455319E-4 0.0 22-23 0.0 0.0 0.0 0.001501317942679681 0.0 24-25 0.0 0.0 0.0 0.0026809248976422872 0.0 26-27 0.0 0.0 0.0 0.004718427819850426 0.0 28-29 0.0 0.0 0.0 0.008042774692926863 0.0 30-31 0.0 0.0 0.0 0.01361909848002282 0.0 32-33 0.0 0.0 0.0 0.019517133254835853 0.0 34-35 0.0 0.0 0.0 0.026916485972328565 0.0 36-37 0.0 0.0 0.0 0.03388689070619851 0.0 38-39 0.0 0.0 0.0 0.03978492548101155 0.0 40-41 0.0 0.0 0.0 0.049007307128901015 0.0 42-43 0.0 0.0 0.0 0.07163431326500191 0.0 44-45 0.0 0.0 0.0 0.10884555084427687 0.0 46-47 0.0 0.0 0.0 0.14487718146858922 0.0 48-49 0.0 0.0 0.0 0.21833452366398787 0.0 50-51 0.0 0.0 0.0 0.2875023860231589 0.0 52-53 0.0 0.0 0.0 0.3576353813454811 0.0 54-55 0.0 0.0 0.0 0.4855691174609711 0.0 56-57 0.0 0.0 0.0 0.608998899748422 0.0 58-59 0.0 0.0 0.0 0.7707122895742048 0.0 60-61 0.0 0.0 0.0 0.9473316218308787 0.0 62-63 0.0 0.0 0.0 1.19569250434846 0.0 64-65 0.0 0.0 0.0 1.369201963723869 0.0 66-67 0.0 0.0 0.0 1.625069435954849 0.0 68-69 0.0 0.0 0.0 1.9512843774999624 0.0 70-71 0.0 0.0 0.0 2.1434530741629616 0.0 72-73 0.0 0.0 0.0 2.4645206399046016 0.0 74-75 0.0 0.0 0.0 2.8296626109634815 0.0 76-77 0.0 0.0 0.0 3.0923932509324255 0.0 78-79 0.0 0.0 0.0 3.3296014858758154 0.0 80-81 0.0 0.0 0.0 3.6972099078405254 0.0 82-83 0.0 0.0 0.0 4.155433591345545 0.0 84-85 0.0 0.0 0.0 4.763681832122628 0.0 86-87 0.0 0.0 0.0 5.527316479967057 0.0 88-89 0.0 0.0 0.0 6.345641995723389 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTGT 50 0.0016146157 38.00103 1 ATTCGTG 35 0.008394056 27.140678 60-61 GAGTACT 4200 0.0 24.425303 12-13 GTACATG 4725 0.0 24.027107 1 GTATCAA 8115 0.0 23.999418 1 GGTATCA 6150 0.0 23.248598 1 TACATGG 4870 0.0 23.114164 2 AGTACTT 4305 0.0 23.05731 12-13 ACATGGG 4770 0.0 23.0013 3 GTACTTT 4445 0.0 22.919987 14-15 GCCTAGA 125 2.2526973E-4 22.800617 1 GTATAGG 210 3.43407E-8 22.619661 1 AGAGTAC 7085 0.0 21.885454 10-11 CATGGGA 3515 0.0 21.755013 4 GACTATC 55 0.002872932 21.596127 34-35 TGTACTA 110 0.002876167 21.589178 5 TACTTTT 4745 0.0 20.82025 14-15 GGGATAG 140 4.8519205E-4 20.353329 9 ACTTTTT 5050 0.0 20.268177 16-17 GTGTGCA 285 1.9590516E-9 19.996252 9 >>END_MODULE