FastQCFastQC Report
Thu 26 May 2016
SRR937085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498931
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94101.8860323371367984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65021.3031862121215159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.939007598245048No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.30144448831601967No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14170.28400720740944135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.2799986370860901No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11620.23289793578671197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.23169536468970658No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11540.23129450765737147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11170.2238786525591715No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.15773724222387464No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7520.15072224415800983No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6580.13188196363825858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.13047896402508563No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.1200566811843722No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5320.10662797060114525No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG250.005004487557.0434722
CGTATAG459.588525E-442.2501871
GGTATCA40450.041.1273421
GTATCAA64900.036.838711
TATCAAC85050.028.28142
TCAACGC85150.028.024884
ATCAACG85500.027.9657573
CAACGCA86600.027.5556415
AACGCAG88700.027.0077296
GTACATG40000.025.7858141
ACATGGG38750.024.6575033
GTCGGGA2701.8189894E-1224.6484152
CATGGGA28750.024.305484
ACGCAGA97850.024.2854827
ATGGGAC4700.024.2738175
TACATGG41400.024.22742
CGCAGAG97850.024.188348
AGTACTT59900.024.03622212-13
GAGTACT59300.023.75857712-13
AGAGTAC80550.023.21523910-11
Produced by FastQC (version 0.11.5)