##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937085_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 498931 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00325696338772 33.0 31.0 34.0 30.0 34.0 2 32.07494623505054 33.0 31.0 34.0 30.0 34.0 3 32.03948842625533 34.0 31.0 34.0 30.0 34.0 4 35.65328873130753 37.0 35.0 37.0 33.0 37.0 5 35.67221920465956 37.0 35.0 37.0 35.0 37.0 6 35.80670072615251 37.0 35.0 37.0 35.0 37.0 7 35.761343752943795 37.0 35.0 37.0 35.0 37.0 8 35.743736107798476 37.0 35.0 37.0 35.0 37.0 9 37.355965053283924 39.0 37.0 39.0 34.0 39.0 10-11 37.25199175837942 39.0 37.0 39.0 34.0 39.0 12-13 37.16472919101038 39.0 37.0 39.0 34.0 39.0 14-15 38.242757014496995 40.0 38.0 41.0 33.5 41.0 16-17 38.224796615163214 40.0 38.0 41.0 33.5 41.0 18-19 38.32236621897617 40.0 38.0 41.0 34.0 41.0 20-21 38.30352393417125 40.0 38.0 41.0 34.0 41.0 22-23 38.160597357149584 40.0 38.0 41.0 34.0 41.0 24-25 38.22001038219714 40.0 38.0 41.0 34.0 41.0 26-27 38.12297391823719 40.0 38.0 41.0 34.0 41.0 28-29 38.00487843008352 40.0 38.0 41.0 33.5 41.0 30-31 37.656088717678394 40.0 37.0 41.0 33.0 41.0 32-33 37.25959902271055 39.5 35.5 41.0 33.0 41.0 34-35 36.9685688000946 39.0 35.0 41.0 32.0 41.0 36-37 36.71840294549747 39.0 35.0 41.0 31.5 41.0 38-39 36.500062132840014 39.0 35.0 41.0 31.0 41.0 40-41 36.21461284225674 39.0 35.0 41.0 30.5 41.0 42-43 35.82102635434559 39.0 35.0 41.0 30.0 41.0 44-45 35.27610030244663 39.0 35.0 40.5 27.0 41.0 46-47 34.51514237439646 38.0 35.0 40.0 22.0 41.0 48-49 33.925835436162515 38.5 34.0 40.0 12.5 41.0 50-51 33.08428319747621 38.0 33.5 39.5 7.5 40.5 52-53 33.235653827884015 38.0 33.5 39.5 6.0 40.5 54-55 33.60490628964726 38.5 34.0 40.5 2.0 41.0 56-57 33.48534967761073 38.0 34.0 41.0 2.0 41.0 58-59 33.241718794783246 38.0 34.0 40.0 2.0 41.0 60-61 32.9703626353143 38.0 34.0 40.0 2.0 41.0 62-63 32.64860772331244 37.5 33.0 40.0 2.0 41.0 64-65 32.31450240614434 37.0 33.0 40.0 2.0 41.0 66-67 31.938198869182315 36.0 32.5 39.5 2.0 41.0 68-69 31.544378882049823 36.0 32.0 39.0 2.0 41.0 70-71 31.141012484692272 35.0 31.5 39.0 2.0 41.0 72-73 30.74703415903201 35.0 31.0 38.5 2.0 40.0 74-75 30.310841579296536 35.0 31.0 37.5 2.0 39.5 76-77 29.892176473299912 35.0 30.5 37.0 2.0 39.0 78-79 29.479157438603735 35.0 30.0 36.5 2.0 39.0 80-81 29.032617736721107 35.0 29.0 36.0 2.0 38.5 82-83 28.62847367672083 34.0 29.0 35.5 2.0 37.0 84-85 28.233611461304267 34.0 29.0 35.0 2.0 37.0 86-87 27.901964399886957 34.0 28.5 35.0 2.0 36.0 88-89 27.547336204805873 34.0 27.0 35.0 2.0 36.0 90-91 27.175682609418935 34.0 26.0 35.0 2.0 36.0 92-93 26.85844736045666 34.0 25.5 35.0 2.0 35.0 94-95 26.524749915318953 34.0 25.0 35.0 2.0 35.0 96-97 26.09797246513045 34.0 23.5 35.0 2.0 35.0 98-99 25.563768336703873 34.0 19.0 35.0 2.0 35.0 100-101 23.416323098785202 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3466104640036285 1101 2 0.3725234561512849 1101 3 0.33923645457382534 1101 4 0.1569388445594413 1101 5 0.4177618367091398 1101 6 0.23075750124232997 1101 7 0.2769410703544253 1101 8 0.30663644119043454 1101 9 0.29882350216903575 1101 10-11 0.3222453366279865 1101 12-13 0.39888749024223813 1101 14-15 0.5264385718040145 1101 16-17 0.5595858389635566 1101 18-19 0.5177400305757871 1101 20-21 0.3836844182617867 1101 22-23 0.42154096707083255 1101 24-25 0.4341411265631123 1101 26-27 0.37002851863405795 1101 28-29 0.5786852051959315 1101 30-31 0.5303686086089741 1101 32-33 0.7122667436120125 1101 34-35 1.014368109634681 1101 36-37 1.0765992437324243 1101 38-39 1.308790687245775 1101 40-41 0.8679250912390657 1101 42-43 1.0064565225006206 1101 44-45 1.1487021090729712 1101 46-47 1.6649991010233904 1101 48-49 1.9958099842562547 1101 50-51 2.0772632555364936 1101 52-53 2.2145286990832105 1101 54-55 2.170971479874197 1101 56-57 1.990582808691272 1101 58-59 2.0164459646043795 1101 60-61 2.2728724828510636 1101 62-63 2.327621719165343 1101 64-65 2.228243590003977 1101 66-67 2.1428477451578622 1101 68-69 2.092995240969117 1101 70-71 2.024984250119413 1101 72-73 1.9998884115054487 1101 74-75 1.977411263109044 1101 76-77 2.105940972360653 1101 78-79 1.8691507290702667 1101 80-81 1.9460000996385958 1101 82-83 1.9399069590146567 1101 84-85 1.746154715184094 1101 86-87 1.9216146010004422 1101 88-89 1.8036378991554969 1101 90-91 1.6548028628763447 1101 92-93 1.7294815524960647 1101 94-95 1.9597124505244885 1101 96-97 1.7002388188979083 1101 98-99 1.5413422136668835 1101 100-101 1.7085721013130346 1102 1 0.13585869702997044 1102 2 0.3025570249397376 1102 3 0.20774562290338494 1102 4 0.24854155805418543 1102 5 0.25089124088965775 1102 6 0.2526524943398414 1102 7 0.3943915388966701 1102 8 0.31664982205304426 1102 9 0.22907761132378823 1102 10-11 0.28689950176607226 1102 12-13 0.17337754365965452 1102 14-15 0.3931696334301975 1102 16-17 0.39843868976587515 1102 18-19 0.42583937552848283 1102 20-21 0.2685358397928823 1102 22-23 0.34605466496022785 1102 24-25 0.23002783667340054 1102 26-27 0.3453916150208016 1102 28-29 0.2531209717539582 1102 30-31 0.4681041255746763 1102 32-33 0.6611968047483501 1102 34-35 0.6711426219541963 1102 36-37 0.33005313913268 1102 38-39 0.3809338942844036 1102 40-41 0.7671381438358225 1102 42-43 0.9879385537622198 1102 44-45 1.1755274517057472 1102 46-47 1.443155638030703 1102 48-49 1.116439202885978 1102 50-51 1.1225980845858956 1102 52-53 1.403882749073766 1102 54-55 1.4131152594695493 1102 56-57 1.5782560866209039 1102 58-59 1.4651194355068355 1102 60-61 1.3645557784027247 1102 62-63 1.59131589753585 1102 64-65 1.7739495206313052 1102 66-67 1.5825013271363169 1102 68-69 1.8495123148225758 1102 70-71 1.9749746014783902 1102 72-73 1.6179199190897116 1102 74-75 1.6184404656223528 1102 76-77 1.9433942400690896 1102 78-79 1.521988383793115 1102 80-81 1.6154949137591146 1102 82-83 1.6908543283285873 1102 84-85 1.6573691928109078 1102 86-87 1.4004968006361267 1102 88-89 1.3040747387272233 1102 90-91 1.5146356763140396 1102 92-93 1.2264883531975528 1102 94-95 1.336032776924231 1102 96-97 1.4998906195887898 1102 98-99 1.4438114175373684 1102 100-101 1.4745946770124583 1103 1 0.3145404931581517 1103 2 0.2734702563706719 1103 3 0.28235627676012953 1103 4 0.15665018595597502 1103 5 0.15229695202825155 1103 6 0.18963808317807462 1103 7 0.2101454695115379 1103 8 0.22552337361545938 1103 9 0.38967879761111135 1103 10-11 0.28977124373765406 1103 12-13 0.471109872830354 1103 14-15 0.5803672153972386 1103 16-17 0.552982773409191 1103 18-19 0.5088204797285698 1103 20-21 0.6037505210819774 1103 22-23 0.5532342384387903 1103 24-25 0.2887870867859519 1103 26-27 0.6254553003792864 1103 28-29 0.4568857074619075 1103 30-31 0.7466172013982799 1103 32-33 0.9241349420936658 1103 34-35 0.9132726502345179 1103 36-37 1.1533896487204487 1103 38-39 1.2489878410719442 1103 40-41 0.9879060397712323 1103 42-43 1.2589389864904632 1103 44-45 1.4069288792718524 1103 46-47 1.5340274761641126 1103 48-49 1.9453235945094036 1103 50-51 2.081982823703221 1103 52-53 1.9573050175313753 1103 54-55 1.6818755586202627 1103 56-57 1.8643451849289008 1103 58-59 1.9892254582971916 1103 60-61 1.9382955120344505 1103 62-63 2.034019837111252 1103 64-65 1.994632182215966 1103 66-67 1.7753997758711328 1103 68-69 1.9788018974365116 1103 70-71 1.908676482316391 1103 72-73 1.709555588135654 1103 74-75 1.8571633053686618 1103 76-77 2.0242794534390818 1103 78-79 2.0874560144092946 1103 80-81 1.9357455027503327 1103 82-83 2.009805639844842 1103 84-85 2.013351551485801 1103 86-87 1.9343588783436267 1103 88-89 1.7740503922998556 1103 90-91 1.8735411361205792 1103 92-93 2.091091401527617 1103 94-95 2.2355185296746782 1103 96-97 2.209266616721422 1103 98-99 1.7090312128875063 1103 100-101 1.9929080421957543 1104 1 0.34262992441937357 1104 2 0.39323846440576204 1104 3 0.35514328630078396 1104 4 0.3518320180873218 1104 5 0.24839403747897393 1104 6 0.2994820207531106 1104 7 0.2684545074124145 1104 8 0.25405960013613793 1104 9 0.53381765616205 1104 10-11 0.3898105075634817 1104 12-13 0.3627101219525528 1104 14-15 0.4933188622213791 1104 16-17 0.487581424899858 1104 18-19 0.34449459900033474 1104 20-21 0.3506522893080657 1104 22-23 0.5260180888210471 1104 24-25 0.3716698639569742 1104 26-27 0.36815538793310765 1104 28-29 0.3384285501628952 1104 30-31 0.44184867744734646 1104 32-33 0.5176505438230805 1104 34-35 0.3250757173260155 1104 36-37 0.35946606424906946 1104 38-39 0.21064017117691947 1104 40-41 0.46247932773303546 1104 42-43 0.5696941156097779 1104 44-45 0.5109870438910846 1104 46-47 1.0236428711943049 1104 48-49 1.2470886614384327 1104 50-51 1.325678046153108 1104 52-53 1.5068564170385557 1104 54-55 1.1748261223043812 1104 56-57 1.3642346143848911 1104 58-59 1.4817585617641384 1104 60-61 1.5099204025921722 1104 62-63 1.4119454434440115 1104 64-65 1.3880399212514263 1104 66-67 1.2692868043667396 1104 68-69 1.2265250468466533 1104 70-71 1.1540189802178098 1104 72-73 1.2971928986989703 1104 74-75 1.3459505495896593 1104 76-77 1.3691941110314403 1104 78-79 1.7471695238732288 1104 80-81 1.7950997707733336 1104 82-83 1.636241928646072 1104 84-85 1.7965836413142107 1104 86-87 1.9373375134066784 1104 88-89 2.086910874838736 1104 90-91 1.9242501415272528 1104 92-93 1.7063668350092804 1104 94-95 1.8160725557964028 1104 96-97 1.8915094386622258 1104 98-99 1.5536976508912765 1104 100-101 1.4438530818657576 1105 1 0.22565963669064715 1105 2 0.27958890051907304 1105 3 0.23359974315157572 1105 4 0.4028322756643803 1105 5 0.25461124059616225 1105 6 0.18519356475080428 1105 7 0.20320165260001488 1105 8 0.25051160241898174 1105 9 0.2779047475110019 1105 10-11 0.39320320146597965 1105 12-13 0.5280872198166833 1105 14-15 0.5866059495299041 1105 16-17 0.5612333120306019 1105 18-19 0.5321369608304494 1105 20-21 0.24426412573148326 1105 22-23 0.3245246469349752 1105 24-25 0.45002632030271883 1105 26-27 0.41208869062656106 1105 28-29 0.49955688919490626 1105 30-31 0.28893881426621704 1105 32-33 0.7287437816677169 1105 34-35 0.5098594788601645 1105 36-37 0.8575557010654151 1105 38-39 0.9459128240690973 1105 40-41 0.9656763135763953 1105 42-43 1.1208105353896727 1105 44-45 1.2034286723841632 1105 46-47 1.2620569540937154 1105 48-49 1.2165771437034962 1105 50-51 1.4078261883154397 1105 52-53 1.3085384962409634 1105 54-55 1.4787582128705026 1105 56-57 1.5537532483173848 1105 58-59 1.5437677133255079 1105 60-61 1.4375690270801798 1105 62-63 1.0695914416561223 1105 64-65 1.593605353400072 1105 66-67 1.7966568920944574 1105 68-69 1.9115966886226232 1105 70-71 1.9878918785657866 1105 72-73 1.8669495641366716 1105 74-75 1.8107003715584575 1105 76-77 1.7899803271234411 1105 78-79 1.5957255771866308 1105 80-81 1.4912686908183836 1105 82-83 1.6705716474830368 1105 84-85 1.6731663513279003 1105 86-87 1.4890271684049345 1105 88-89 1.239012653130544 1105 90-91 1.4594030968452074 1105 92-93 1.5841035912555235 1105 94-95 1.4112838952476139 1105 96-97 1.2726111108928961 1105 98-99 1.4324520890450074 1105 100-101 1.3245216711010563 1106 1 0.11391263167142895 1106 2 0.11718444614686518 1106 3 0.10556636518306917 1106 4 0.19788224420105394 1106 5 0.07916814448538645 1106 6 0.09806251327918858 1106 7 0.008856701196691574 1106 8 -0.04488878707017818 1106 9 -0.06695718663372219 1106 10-11 0.158986310281378 1106 12-13 0.18652818954329575 1106 14-15 0.08939738792589225 1106 16-17 0.04141290649663887 1106 18-19 -0.07401672932585512 1106 20-21 0.20047343589428834 1106 22-23 -0.10660794340652302 1106 24-25 -0.0029233294254495945 1106 26-27 -0.2537946236697195 1106 28-29 0.22748707937655865 1106 30-31 -1.830315611783817E-5 1106 32-33 -0.06242173333179579 1106 34-35 -0.064654927168867 1106 36-37 0.32744932221115874 1106 38-39 0.1487871705713104 1106 40-41 0.13867540993769012 1106 42-43 -0.04455982227828059 1106 44-45 0.17351017880655206 1106 46-47 0.18533116818152706 1106 48-49 0.4808899756714311 1106 50-51 0.4400664070974045 1106 52-53 0.524760153150531 1106 54-55 0.13905119460983428 1106 56-57 0.20888684071369568 1106 58-59 0.062260992698988105 1106 60-61 0.3809365276989993 1106 62-63 0.6439582235392081 1106 64-65 0.41278366845684644 1106 66-67 0.39512517974383243 1106 68-69 0.15403281508432443 1106 70-71 0.5360889254864034 1106 72-73 0.7574899878030656 1106 74-75 0.8901460205231508 1106 76-77 0.5727148631213659 1106 78-79 0.7617848475356475 1106 80-81 0.7644866884385024 1106 82-83 0.7726813307323219 1106 84-85 0.9657529351262255 1106 86-87 0.9913727520571278 1106 88-89 0.9182000343974863 1106 90-91 0.7261629108686094 1106 92-93 0.5914818162466524 1106 94-95 0.5209969675894115 1106 96-97 0.8610633239130436 1106 98-99 0.9897660453422965 1106 100-101 1.180689527210756 1107 1 0.05000688609333537 1107 2 -0.048898826082119484 1107 3 -0.19097606888230345 1107 4 0.010634195945762315 1107 5 -0.04753849161251367 1107 6 0.0682446982378977 1107 7 -0.07818796082715096 1107 8 0.18802992147701758 1107 9 -0.03408028341304714 1107 10-11 0.11018522732435088 1107 12-13 0.04740105821817053 1107 14-15 0.0730402244504802 1107 16-17 0.1634114665353863 1107 18-19 -0.11894086592973707 1107 20-21 0.3152887791126986 1107 22-23 0.1330427498651332 1107 24-25 0.015792848904133905 1107 26-27 -0.13085710272558515 1107 28-29 0.057468797840911634 1107 30-31 0.009372964839251097 1107 32-33 0.2535585186282461 1107 34-35 0.004055113077050976 1107 36-37 0.17933902250677392 1107 38-39 0.0670776645045521 1107 40-41 0.10099752993225053 1107 42-43 0.2289233245347333 1107 44-45 0.12198843174205365 1107 46-47 0.10251017129039042 1107 48-49 0.09162266378710626 1107 50-51 -0.0035362166845160914 1107 52-53 0.1412377557809421 1107 54-55 0.41078747599286203 1107 56-57 0.35313180665562527 1107 58-59 0.21750639991937248 1107 60-61 0.28466151500810355 1107 62-63 0.3211746523689598 1107 64-65 0.09747196993297536 1107 66-67 0.3992376957940955 1107 68-69 0.24985161885476614 1107 70-71 0.28634523195262673 1107 72-73 0.4990451459499603 1107 74-75 0.4390433220888603 1107 76-77 0.10606290573625543 1107 78-79 -0.2576612650666661 1107 80-81 -0.14300741402436046 1107 82-83 0.027002043944463594 1107 84-85 -0.05875958490549493 1107 86-87 -0.1001344626795806 1107 88-89 -0.18468088346168088 1107 90-91 -0.16854904711372143 1107 92-93 -0.22648252978200745 1107 94-95 -0.4328064876540054 1107 96-97 -0.31984424981860826 1107 98-99 -0.43589346686253805 1107 100-101 -0.18089336843850035 1108 1 -0.08036776223110209 1108 2 -0.07584206547787886 1108 3 -0.030328648511094514 1108 4 0.179556533837669 1108 5 0.12254091790988042 1108 6 -0.08090284694861793 1108 7 -0.19994697957643126 1108 8 -0.05977906387573739 1108 9 -0.18965075683414057 1108 10-11 -0.0805561755997104 1108 12-13 0.051970310473279824 1108 14-15 -0.1600844086937201 1108 16-17 -0.0426906526261277 1108 18-19 0.04437490330362692 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46-47 -1.8069932109897664 2316 48-49 -2.04259611199247 2316 50-51 -2.254113679149455 2316 52-53 -1.8694452998204412 2316 54-55 -1.7779602554045937 2316 56-57 -1.9847662299719957 2316 58-59 -1.9240956459476521 2316 60-61 -1.719463216884325 2316 62-63 -1.6890959300431838 2316 64-65 -1.6218812721063003 2316 66-67 -1.701653246294672 2316 68-69 -1.7276685923315043 2316 70-71 -1.7106024664469786 2316 72-73 -2.2146984607156064 2316 74-75 -2.361690880187993 2316 76-77 -2.5883769556727536 2316 78-79 -2.8008884552992974 2316 80-81 -2.7236871698402325 2316 82-83 -2.583994491280748 2316 84-85 -2.209889761444021 2316 86-87 -2.658004451130335 2316 88-89 -2.6232636255834443 2316 90-91 -2.4049786791876357 2316 92-93 -3.038848475995504 2316 94-95 -2.2117208582199197 2316 96-97 -2.615175053316385 2316 98-99 -2.556913100586346 2316 100-101 -2.5460146355561406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 919.0 3 283.0 4 294.0 5 256.0 6 344.0 7 370.0 8 572.0 9 676.0 10 957.0 11 1531.0 12 2457.0 13 3410.0 14 4015.0 15 5595.0 16 7986.0 17 11650.0 18 9590.0 19 4346.0 20 3733.0 21 3617.0 22 3658.0 23 3933.0 24 4126.0 25 4392.0 26 5269.0 27 5836.0 28 6554.0 29 7991.0 30 9568.0 31 11984.0 32 14958.0 33 19966.0 34 28048.0 35 42460.0 36 61574.0 37 93599.0 38 93837.0 39 18570.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.2962887966206 19.477051326518133 16.230976348621756 18.99568352823951 2 14.842833861530227 21.666696734801057 42.00879192249637 21.481677481172337 3 17.76654325170038 29.419735837180443 32.47951826067085 20.334202650448326 4 11.109507284380532 17.994735438756873 40.328824575437 30.566932701425603 5 10.996556682382527 38.82125030332079 34.402092061117386 15.780100953179302 6 26.023636888625095 38.99505125202521 18.96344984680537 16.017862012544313 7 23.579727692603758 36.969978831227145 22.7997305792546 16.650562896914494 8 25.63245450097455 32.327167871150934 21.3113504928894 20.72902713498512 9 27.433557348388852 15.870450863714373 22.256884854122895 34.43910693377388 10-11 23.068503037228606 27.88987790942443 27.746937711753972 21.29468134159299 12-13 26.262660692928257 26.564923301489657 28.76497402947637 18.407441976105716 14-15 22.901282890417562 25.721456715604074 30.72121114317474 20.656049250803623 16-17 18.364755162324677 29.6772150058988 30.490016147769634 21.46801368400689 18-19 18.17234166031026 25.973632854589795 36.95930863672857 18.894716848371374 20-21 20.122019057584698 24.561371857437962 36.94569480401679 18.370914280960555 22-23 19.359539458518995 24.982310765277386 37.21173562962651 18.44641414657711 24-25 18.90750239015796 25.081425716696632 37.380968129222865 18.630103763922545 26-27 18.611409805574123 25.054892414875418 37.65053449328113 18.68316328626933 28-29 18.189516597784824 25.46820945959489 37.53322162582528 18.80905231679501 30-31 18.32958866055627 25.157787349352915 37.500476017725894 19.012147972364914 32-33 18.35425151524869 25.404066318186192 37.115375364782096 19.126306801783024 34-35 18.810454372356844 25.380313871685406 36.954983665043194 18.854248090914556 36-37 18.817732311682377 25.01097346126017 36.9616239520094 19.20967027504805 38-39 18.67135670334516 25.599883751227626 36.566251778806645 19.162507766620568 40-41 18.631152034201158 25.78755958996293 36.4625934874697 19.118694888366218 42-43 18.556792980184614 25.94459548975258 35.54553189271852 19.953079637344278 44-45 18.74740069007607 25.917337184237084 35.25811711250365 20.0771450131832 46-47 19.46541706167787 26.380902369265492 34.46959599623996 19.684084572816683 48-49 19.48521785141989 26.870529804311026 33.183846615604004 20.46040572866507 50-51 19.22191567763446 27.38297217748765 32.967918377409276 20.427193767468616 52-53 19.59072004810342 27.76579646239415 31.919126121160495 20.724357368341938 54-55 19.204027066246564 27.36829657422774 32.296101209803496 21.131575149722202 56-57 19.627121667490098 27.40246868545556 31.82654062347486 21.143869023579484 58-59 20.04507687019405 27.00964372436366 32.16913146351509 20.7761479419272 60-61 19.917060269604235 27.163563441978766 32.766204364858645 20.153171923558354 62-63 19.848253137257654 27.14266530105848 32.866694860730014 20.142386700953853 64-65 19.716570910887494 27.330633531459103 32.692756734221845 20.260038823431554 66-67 19.683855630344407 27.288097882081274 32.18430663518319 20.843739852391128 68-69 19.706056864285827 27.19014095800125 32.50563270738787 20.598169470325054 70-71 19.95088359411806 27.28947605825807 32.044044385192905 20.71559596243096 72-73 19.876214674825473 27.399054821363244 31.99655205248097 20.72817845133032 74-75 19.33316962428774 28.110914548889703 31.62217288677515 20.93374294004741 76-77 19.192090316190875 27.748450722862337 31.836918407076453 21.222540553870335 78-79 19.277099980354343 27.535592717475417 31.852124336959093 21.33518296521115 80-81 19.351517187100526 27.79442653686316 31.396072808939195 21.457983467097115 82-83 19.400797573196087 27.590057803004637 31.592660241437315 21.416484382361965 84-85 19.25404579724206 27.512924700962817 31.090846765867962 22.14218273592716 86-87 19.1787865211783 27.71585468402184 31.205029752150605 21.900329042649258 88-89 19.275138914502435 27.108373357280886 31.111035384109627 22.505452344107056 90-91 18.93836728638844 27.06685297077538 31.51098779935288 22.4837919434833 92-93 19.272092640932183 27.38178296904885 31.361946806847023 21.98417758317194 94-95 18.86452062949889 27.235288339918174 31.369807546209156 22.53038348437378 96-97 18.676748733811177 27.55305063051342 31.462925449969383 22.307275185706025 98-99 18.793400210716886 27.770220350519825 30.89358195653939 22.542797482223893 100-101 18.789312116904235 27.631919283464303 31.023012313201654 22.555756286429812 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1008.0 1 729.5 2 575.5 3 667.0 4 516.5 5 474.0 6 872.5 7 2051.0 8 3603.5 9 3997.0 10 2996.5 11 2154.0 12 2026.5 13 2067.0 14 2141.5 15 2192.5 16 2234.0 17 2279.5 18 2328.5 19 2383.0 20 2542.0 21 2717.0 22 2926.0 23 3349.0 24 3645.5 25 4105.5 26 4766.5 27 5402.5 28 6122.5 29 6994.5 30 8072.5 31 8941.0 32 9982.0 33 11246.5 34 12367.0 35 12942.0 36 14242.5 37 15945.5 38 16488.0 39 16561.5 40 16217.0 41 16009.5 42 15642.0 43 15611.5 44 16205.5 45 16596.0 46 16625.0 47 17468.5 48 19865.5 49 19017.0 50 15181.5 51 12888.5 52 11788.5 53 11101.5 54 10394.0 55 9535.0 56 8538.0 57 7396.0 58 6736.5 59 6274.0 60 5622.5 61 4963.0 62 4038.5 63 2817.5 64 1798.0 65 1240.0 66 869.0 67 640.0 68 475.5 69 326.5 70 240.0 71 172.5 72 128.5 73 114.5 74 76.0 75 53.5 76 44.0 77 20.5 78 12.0 79 9.5 80 5.5 81 2.0 82 2.0 83 3.5 84 1.5 85 1.0 86 2.5 87 2.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028861706328129542 2 0.012827425034724239 3 0.014430853164064771 4 0.024853136004778214 5 0.05692169859158882 6 0.04389384504069701 7 0.016635566841908 8 0.04790241536404834 9 0.031066420005972768 10-11 0.02425185045627552 12-13 0.03858248936225651 14-15 0.019341351810170142 16-17 0.020143065874840407 18-19 0.018138780713164746 20-21 0.014931924454483686 22-23 0.00801714064670265 24-25 0.0020042851616756626 26-27 1.0021425808378312E-4 28-29 0.0018038566455080964 30-31 0.0 32-33 6.012855485026988E-4 34-35 2.0042851616756624E-4 36-37 0.0 38-39 2.0042851616756624E-4 40-41 1.0021425808378312E-4 42-43 1.0021425808378312E-4 44-45 1.0021425808378312E-4 46-47 0.0 48-49 3.006427742513494E-4 50-51 1.0021425808378312E-4 52-53 0.0012025710970053976 54-55 0.0014029996131729638 56-57 3.006427742513494E-4 58-59 0.0011023568389216143 60-61 0.0029062134844297107 62-63 0.0016034281293405299 64-65 0.0011023568389216143 66-67 0.00901928322754048 68-69 0.011825282453886409 70-71 0.023249707875437686 72-73 0.016735781099991784 74-75 0.01573363851915395 76-77 0.030064277425134938 78-79 0.018639852003583663 80-81 0.04579791594428889 82-83 0.03367199071615113 84-85 0.0160342812934053 86-87 0.011925496711970194 88-89 0.012626996518556673 90-91 0.021044994197594456 92-93 0.029062134844297104 94-95 0.011624853937718843 96-97 0.018339209229332312 98-99 0.031567491296391685 100-101 0.03968484620117812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 498931.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.07923410454312 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28901784974927 43.12099141961095 2 12.250540802167313 13.495008094996615 3 4.038543577058663 6.673196613666392 4 1.7359289988160727 3.8245455885862243 5 0.951441100697959 2.6202323561013525 6 0.5748072116395805 1.8995964584925669 7 0.37822588893782233 1.4582674596843668 8 0.287886866198052 1.2685270479156627 9 0.207798106436721 1.0300824495818028 >10 1.1792013453936505 11.898790898992653 >50 0.06840393026767988 2.5525734485313034 >100 0.0323827115686912 3.4479882291443587 >500 0.0021831041506982832 0.7974161676120549 >1k 0.0029108055342643778 2.7239207213327594 >5k 7.277013835660944E-4 3.188863045750947 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9410 1.8860323371367984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6502 1.3031862121215159 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4685 0.939007598245048 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1504 0.30144448831601967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1417 0.28400720740944135 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1397 0.2799986370860901 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1162 0.23289793578671197 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1156 0.23169536468970658 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1154 0.23129450765737147 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1117 0.2238786525591715 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 787 0.15773724222387464 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 752 0.15072224415800983 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 658 0.13188196363825858 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.13047896402508563 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 599 0.1200566811843722 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 532 0.10662797060114525 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.010712904189157E-4 0.0 22-23 0.0 0.0 0.0 0.001503213871256747 0.0 24-25 0.0 0.0 0.0 0.003006427742513494 0.0 26-27 0.0 0.0 0.0 0.0038081418071837588 0.0 28-29 0.0 0.0 0.0 0.0069147838077810355 0.0 30-31 0.0 0.0 0.0 0.012626996518556675 0.0 32-33 0.0 0.0 0.0 0.01894049477783501 0.0 34-35 0.0 0.0 0.0 0.024051421940107953 0.0 36-37 0.0 0.0 0.0 0.028661277811961976 0.0 38-39 0.0 0.0 0.0 0.035275418845491666 0.0 40-41 0.0 0.0 0.0 0.04349298800836188 0.0 42-43 0.0 0.0 0.0 0.06493883923829147 0.0 44-45 0.0 0.0 0.0 0.09780911588977233 0.0 46-47 0.0 0.0 0.0 0.1294768214442478 0.0 48-49 0.0 0.0 0.0 0.2030340868777446 0.0 50-51 0.0 0.0 0.0 0.2695763542453766 0.0 52-53 0.0 0.0 0.0 0.3371207641938464 0.0 54-55 0.0 0.0 0.0 0.45186608969977815 0.0 56-57 0.0 0.0 0.0 0.5598970599140963 0.0 58-59 0.0 0.0 0.0 0.6980925218116333 0.0 60-61 0.0 0.0 0.0 0.8691582603606511 0.0 62-63 0.0 0.0 0.0 1.106666052019217 0.0 64-65 0.0 0.0 0.0 1.2799365042460782 0.0 66-67 0.0 0.0 0.0 1.5180455814531468 0.0 68-69 0.0 0.0 0.0 1.8097692867350395 0.0 70-71 0.0 0.0 0.0 2.000376805610395 0.0 72-73 0.0 0.0 0.0 2.282379727858161 0.0 74-75 0.0 0.0 0.0 2.6137882793412315 0.0 76-77 0.0 0.0 0.0 2.8707376370680517 0.0 78-79 0.0 0.0 0.0 3.1118531420176336 0.0 80-81 0.0 0.0 0.0 3.4724240426030857 0.0 82-83 0.0 0.0 0.0 3.918878562366339 0.0 84-85 0.0 0.0 0.0 4.489197905121149 0.0 86-87 0.0 0.0 0.0 5.205228779129779 0.0 88-89 0.0 0.0 0.0 5.970364639599463 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 0.0050044875 57.043472 2 CGTATAG 45 9.588525E-4 42.250187 1 GGTATCA 4045 0.0 41.127342 1 GTATCAA 6490 0.0 36.83871 1 TATCAAC 8505 0.0 28.2814 2 TCAACGC 8515 0.0 28.02488 4 ATCAACG 8550 0.0 27.965757 3 CAACGCA 8660 0.0 27.555641 5 AACGCAG 8870 0.0 27.007729 6 GTACATG 4000 0.0 25.785814 1 ACATGGG 3875 0.0 24.657503 3 GTCGGGA 270 1.8189894E-12 24.648415 2 CATGGGA 2875 0.0 24.30548 4 ACGCAGA 9785 0.0 24.285482 7 ATGGGAC 470 0.0 24.273817 5 TACATGG 4140 0.0 24.2274 2 CGCAGAG 9785 0.0 24.18834 8 AGTACTT 5990 0.0 24.036222 12-13 GAGTACT 5930 0.0 23.758577 12-13 AGAGTAC 8055 0.0 23.215239 10-11 >>END_MODULE