FastQCFastQC Report
Thu 26 May 2016
SRR937085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498931
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63201.2667082221790187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.9913194409647826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.7014998065864819No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28680.57482898436858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19420.3892321783974137No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.24432236120826328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.21646279746097155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.17657752274362587No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8010.1605432414502206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7720.15473081448136114No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6370.1276729647987397No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5970.11965582415203704No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.10021425808378313No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG600.00394752131.6688045
GTATCAA80450.027.7464681
CGATCGT350.008378033527.15149524-25
CCGCTAG350.00838418327.14741182-83
GGTTGCG700.00838433427.1446917
TAAGGGT908.952601E-426.3827363
GTACTAC1109.4746494E-525.9056471
GTGTTAA1858.954885E-925.6722621
GGTATCA57700.024.7757361
ATAGGAC1351.3431725E-524.6238863
AGTACTT47950.023.71970412-13
GAGTACT47600.023.44516212-13
ATCAACG98150.022.692113
TTGATCG1050.002193630422.6205739
GTCTATA1050.002196188322.616041
TCAACGC98500.022.6114794
ATGGGAG11800.022.5438965
GTACATG50200.022.5169261
TATCAAC99200.022.4519212
CAACGCA99200.022.410795
Produced by FastQC (version 0.11.5)