FastQCFastQC Report
Thu 26 May 2016
SRR937084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519331
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97411.8756823682776493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68761.3240110834901055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.9258064702472989No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.30000134788795585No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.281901138195101No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14480.2788202514388704No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13270.2555210453448764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.23626550311843506No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11800.22721539827200765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11760.22644517658295No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8240.15866566794587653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.1394101257194352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.12496846904960421No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6380.12285035940469566No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6030.11611091962544118No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.113222588291475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.039.1754041
GTATCAA67900.036.9657631
ATCAACG86050.029.1716123
TATCAAC86450.029.0366382
TCAACGC86750.028.8814184
CAACGCA87800.028.5360245
GTCCTAC1004.9214592E-528.5224461
AACGCAG88900.028.2871656
GTACATG43150.028.202961
TACATGG44300.027.5807952
GGATAGG700.00835525527.1642321
CACGCCG559.443729E-525.92199714-15
ACATGGG44650.025.5544873
ACGCAGA99000.025.3003867
CGCAGAG98700.025.2328268
GAGTACT58350.024.88650912-13
CATGGGG10700.024.4374734
GCAGAGT100600.023.9058559
ATAGGAC1401.764928E-523.7709963
AGTACTT59800.023.68692812-13
Produced by FastQC (version 0.11.5)