##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519331 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.953792475319208 33.0 31.0 34.0 30.0 34.0 2 31.988371578049453 33.0 31.0 34.0 30.0 34.0 3 31.957343197305764 33.0 31.0 34.0 30.0 34.0 4 35.60545971644289 37.0 35.0 37.0 33.0 37.0 5 35.63545985123168 37.0 35.0 37.0 33.0 37.0 6 35.74594622697278 37.0 35.0 37.0 35.0 37.0 7 35.708569293957034 37.0 35.0 37.0 33.0 37.0 8 35.69007819675698 37.0 35.0 37.0 33.0 37.0 9 37.31674981851651 39.0 37.0 39.0 34.0 39.0 10-11 37.20665144195128 39.0 37.0 39.0 34.0 39.0 12-13 37.113007888995654 39.0 37.0 39.0 33.5 39.0 14-15 38.159326133044246 40.0 38.0 41.0 33.0 41.0 16-17 38.15860501298786 40.0 38.0 41.0 33.0 41.0 18-19 38.26621846182878 40.0 38.0 41.0 33.5 41.0 20-21 38.272578567426166 40.0 38.0 41.0 34.0 41.0 22-23 38.16281427451857 40.0 38.0 41.0 34.0 41.0 24-25 38.09682553130855 40.0 38.0 41.0 33.0 41.0 26-27 38.049534882377515 40.0 38.0 41.0 33.0 41.0 28-29 37.93246696230342 40.0 37.5 41.0 33.0 41.0 30-31 37.61402554440232 40.0 37.0 41.0 33.0 41.0 32-33 37.228996535927955 39.0 35.5 41.0 33.0 41.0 34-35 36.968564364538224 39.0 35.0 41.0 32.0 41.0 36-37 36.72628246725114 39.0 35.0 41.0 31.0 41.0 38-39 36.496541704616135 39.0 35.0 41.0 31.0 41.0 40-41 36.21715919134425 39.0 35.0 41.0 30.0 41.0 42-43 35.82867958970291 39.0 35.0 41.0 30.0 41.0 44-45 35.298843127023034 39.0 35.0 40.5 27.0 41.0 46-47 34.5629088192309 38.0 35.0 40.0 22.5 41.0 48-49 33.98720758052187 38.5 34.0 40.0 15.0 41.0 50-51 33.157727923039445 38.0 33.5 39.5 8.5 40.5 52-53 33.30040763982893 38.0 33.5 40.0 7.0 40.5 54-55 33.64434724674629 38.5 34.0 40.5 3.5 41.0 56-57 33.507751318523255 38.0 34.0 41.0 2.0 41.0 58-59 33.29286428116173 38.0 34.0 40.0 2.0 41.0 60-61 33.01075614588769 38.0 33.5 40.0 2.0 41.0 62-63 32.71035139439009 37.5 33.0 40.0 2.0 41.0 64-65 32.381882652874566 37.0 33.0 40.0 2.0 41.0 66-67 32.00838097475406 36.0 32.5 39.5 2.0 41.0 68-69 31.61498254485097 36.0 32.0 39.0 2.0 41.0 70-71 31.19537924753192 35.0 31.5 39.0 2.0 40.5 72-73 30.812875603420558 35.0 31.0 38.5 2.0 40.0 74-75 30.420022105362477 35.0 31.0 37.5 2.0 40.0 76-77 29.98793255168669 35.0 30.5 37.0 2.0 39.0 78-79 29.524310122060882 35.0 30.0 36.5 2.0 39.0 80-81 29.100856679073654 35.0 29.5 36.0 2.0 38.5 82-83 28.706371273811882 34.5 29.0 35.5 2.0 37.0 84-85 28.32766097151913 34.0 29.0 35.0 2.0 37.0 86-87 28.009147345334668 34.0 29.0 35.0 2.0 36.0 88-89 27.66230207709534 34.0 27.5 35.0 2.0 36.0 90-91 27.30522730204821 34.0 26.5 35.0 2.0 36.0 92-93 26.961491803878452 34.0 26.0 35.0 2.0 35.0 94-95 26.6415840764368 34.0 25.0 35.0 2.0 35.0 96-97 26.23101836786173 34.0 24.5 35.0 2.0 35.0 98-99 25.789819979935725 34.0 22.0 35.0 2.0 35.0 100-101 23.91448228586393 31.0 8.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12588849088293586 1101 2 0.1800200438234043 1101 3 0.2396894705990853 1101 4 0.20501838267119865 1101 5 0.12951084407492885 1101 6 0.1623487903024312 1101 7 -0.011742917185763702 1101 8 0.11596023676067091 1101 9 -0.024779448865906772 1101 10-11 0.08432621373384563 1101 12-13 0.09793288736606343 1101 14-15 0.23836644607083457 1101 16-17 0.26723099961629515 1101 18-19 0.36898435228738435 1101 20-21 0.21942816820401845 1101 22-23 0.292416850481068 1101 24-25 0.3965991322710636 1101 26-27 0.455032882727771 1101 28-29 0.5276320560028367 1101 30-31 0.48993197169208713 1101 32-33 0.5616345114833905 1101 34-35 0.6995097730027808 1101 36-37 0.8660179145164975 1101 38-39 0.9291870825166342 1101 40-41 0.9898830190747034 1101 42-43 1.163941383570993 1101 44-45 1.2360168967842569 1101 46-47 1.3186980152780379 1101 48-49 1.3520455543016823 1101 50-51 1.3409682650313215 1101 52-53 1.3673988740758247 1101 54-55 1.4019014694307899 1101 56-57 1.4614871884912048 1101 58-59 1.5918504003026825 1101 60-61 1.6427992010212975 1101 62-63 1.4973501152414173 1101 64-65 1.4804724376777116 1101 66-67 1.3725598842333007 1101 68-69 1.4634753057339545 1101 70-71 1.5069631235358258 1101 72-73 1.4116247928155694 1101 74-75 1.321637710540628 1101 76-77 1.5890958058757185 1101 78-79 1.653337151990403 1101 80-81 1.5920369002257537 1101 82-83 1.3752039245561285 1101 84-85 1.4576742763250223 1101 86-87 1.414245410914436 1101 88-89 1.3918049299440156 1101 90-91 1.36453600666972 1101 92-93 1.4486499619905402 1101 94-95 1.584057606223201 1101 96-97 1.4684220097499932 1101 98-99 1.4480737959950183 1101 100-101 1.6064545635870466 1102 1 0.22622840872423922 1102 2 0.13049264978550923 1102 3 0.2832413167256682 1102 4 0.07867240781575191 1102 5 0.1470729264332178 1102 6 0.06030419161525913 1102 7 0.16996343371779687 1102 8 -0.0022289318968304883 1102 9 0.1211489708318112 1102 10-11 0.17510606957934982 1102 12-13 0.14478818908642666 1102 14-15 0.21411394964007258 1102 16-17 0.42640582818079764 1102 18-19 0.22495307740199877 1102 20-21 0.2220860039314374 1102 22-23 0.15194564981141667 1102 24-25 0.030314745884879812 1102 26-27 0.07989719053976785 1102 28-29 -0.04133318032051392 1102 30-31 0.19449367319575117 1102 32-33 0.21784804261672974 1102 34-35 0.3135574022336769 1102 36-37 0.18822092428634107 1102 38-39 0.25768437681949763 1102 40-41 -0.0015172021061005125 1102 42-43 0.3701476845764873 1102 44-45 0.26201582550334734 1102 46-47 0.4701396620363951 1102 48-49 0.5477070702660072 1102 50-51 0.569941322112669 1102 52-53 0.8517828162673879 1102 54-55 0.8041022796124437 1102 56-57 0.9740319694590696 1102 58-59 0.7739213125818836 1102 60-61 0.9364096010001148 1102 62-63 0.8034778013357382 1102 64-65 0.9968980661387548 1102 66-67 0.7212567300645709 1102 68-69 0.849032565291477 1102 70-71 0.8439288323633107 1102 72-73 0.7760374925860347 1102 74-75 1.217740773467181 1102 76-77 1.0080155229435057 1102 78-79 0.8359549063330611 1102 80-81 0.7858267295743708 1102 82-83 0.9188148336988817 1102 84-85 1.0721618231935643 1102 86-87 1.177321249863848 1102 88-89 1.122483686199093 1102 90-91 0.9023923450478506 1102 92-93 1.0263799462471894 1102 94-95 1.1237072199714397 1102 96-97 0.6487566730195162 1102 98-99 0.6541059629356205 1102 100-101 0.2685295899108233 1103 1 0.2542609032255072 1103 2 0.2577732835417308 1103 3 0.31138094980753905 1103 4 0.3530543127397152 1103 5 0.2910671787634769 1103 6 0.20380686692864458 1103 7 0.32610846154231865 1103 8 0.18002639824755562 1103 9 0.2150023674399293 1103 10-11 0.20142434679097931 1103 12-13 0.3357721624152177 1103 14-15 0.6773023937988185 1103 16-17 0.6075299009594133 1103 18-19 0.49527632080194905 1103 20-21 0.3027550528559573 1103 22-23 0.3828481978189515 1103 24-25 0.5648511824556905 1103 26-27 0.7820613898679483 1103 28-29 0.7705062630630479 1103 30-31 0.7497582739784932 1103 32-33 0.6717343370713635 1103 34-35 0.7354061099533311 1103 36-37 0.5950324171048962 1103 38-39 0.8800649960804918 1103 40-41 0.8051134155793207 1103 42-43 0.6333399100969004 1103 44-45 0.6390396417795827 1103 46-47 0.8527788003901122 1103 48-49 0.7859297042365014 1103 50-51 0.9367649152885775 1103 52-53 1.0337450908824763 1103 54-55 1.0944541353156794 1103 56-57 1.319103279637858 1103 58-59 1.1767497711098045 1103 60-61 0.977308178577907 1103 62-63 1.121398934735879 1103 64-65 1.094781764058375 1103 66-67 1.1369796318815126 1103 68-69 1.2731469388773995 1103 70-71 1.1792599851114147 1103 72-73 1.0634713975352064 1103 74-75 0.9017670413262344 1103 76-77 0.9687901979039601 1103 78-79 1.192499498040533 1103 80-81 1.110683510501186 1103 82-83 0.9841945769444216 1103 84-85 1.1271396707164563 1103 86-87 1.204317021041053 1103 88-89 1.25657707322776 1103 90-91 1.1121098143085284 1103 92-93 1.2849715150711205 1103 94-95 1.3558040480392286 1103 96-97 1.5214539813202386 1103 98-99 1.4074004742891582 1103 100-101 1.8786365394233435 1104 1 0.12188396650142863 1104 2 -0.12443346653210696 1104 3 0.08797038839058402 1104 4 0.2174056031210796 1104 5 0.1522221020276291 1104 6 -0.06930238556969925 1104 7 -0.07110613282696221 1104 8 -0.09928497943694481 1104 9 0.26359301251540757 1104 10-11 0.05560004644139127 1104 12-13 0.14129018963252093 1104 14-15 0.14672639839019297 1104 16-17 0.32979052427194233 1104 18-19 0.29554945728430937 1104 20-21 0.24207749005447 1104 22-23 0.15705397599609938 1104 24-25 0.0623858324576787 1104 26-27 -0.00220582307168371 1104 28-29 -0.03491122311779549 1104 30-31 0.2605632934897386 1104 32-33 0.21846078376261602 1104 34-35 0.41203995416080375 1104 36-37 0.23212221491268537 1104 38-39 0.32544817263264747 1104 40-41 0.28884147721098685 1104 42-43 0.46083767410614485 1104 44-45 0.7529934808762206 1104 46-47 0.6750306263619592 1104 48-49 0.5381117589182551 1104 50-51 0.5407253942828305 1104 52-53 0.5775338541648907 1104 54-55 0.7946596160589294 1104 56-57 0.7119862310199849 1104 58-59 0.7333449489103359 1104 60-61 0.8991699891125151 1104 62-63 0.6752407543610772 1104 64-65 0.8580369476819172 1104 66-67 0.7094770502809418 1104 68-69 0.7407612048126317 1104 70-71 0.9081709790642876 1104 72-73 0.8341823273931759 1104 74-75 0.9162334302482869 1104 76-77 0.9449085598391243 1104 78-79 0.9233511434574346 1104 80-81 0.96234777884521 1104 82-83 0.9108760734139665 1104 84-85 0.6842375536992584 1104 86-87 0.8296508487011458 1104 88-89 0.8741074502357016 1104 90-91 0.9807382879489772 1104 92-93 0.8857838028614609 1104 94-95 1.0182469438724304 1104 96-97 0.9836379839845755 1104 98-99 1.028443503758151 1104 100-101 0.7877202126063274 1105 1 0.15895210053513864 1105 2 0.33072787676562854 1105 3 0.2246015967710413 1105 4 0.29334138499595497 1105 5 0.24437895116684416 1105 6 0.1685364399815441 1105 7 0.1251172011771402 1105 8 0.1951529500582012 1105 9 0.19706882663349745 1105 10-11 0.2089808425296411 1105 12-13 0.12873638347577554 1105 14-15 0.2632715169198647 1105 16-17 0.2790409967021432 1105 18-19 0.3968079604496495 1105 20-21 0.2699526065718274 1105 22-23 0.29296352845377527 1105 24-25 0.4107108736869023 1105 26-27 0.40822673101202867 1105 28-29 0.35002744769376903 1105 30-31 0.4646149751562092 1105 32-33 0.4626441676349984 1105 34-35 0.5523789011445359 1105 36-37 0.3238766020949697 1105 38-39 0.7430174874527324 1105 40-41 0.8151345018976244 1105 42-43 0.9450445117960129 1105 44-45 0.7973938241253862 1105 46-47 0.9613360487272757 1105 48-49 0.9991862860832796 1105 50-51 0.7759563126131184 1105 52-53 0.8162203941254305 1105 54-55 0.6331451563409729 1105 56-57 0.9581611076792669 1105 58-59 0.8357345089546584 1105 60-61 0.8178183183171441 1105 62-63 0.8280618045092254 1105 64-65 0.8392542331916744 1105 66-67 0.9172954520443746 1105 68-69 0.6393708606613977 1105 70-71 0.7585303035758386 1105 72-73 0.5823406013402384 1105 74-75 0.6787334591607852 1105 76-77 0.3862925201110379 1105 78-79 0.6146292839906735 1105 80-81 0.552757156671408 1105 82-83 0.3700504712823083 1105 84-85 0.5182196834631689 1105 86-87 0.6773421279981804 1105 88-89 0.8458379672605361 1105 90-91 0.800896774692017 1105 92-93 0.6956563797050954 1105 94-95 0.6824826477992119 1105 96-97 0.7066169426258249 1105 98-99 0.46194013487401264 1105 100-101 0.2739439860803792 1106 1 0.017413419709853883 1106 2 -0.1429414862152818 1106 3 -0.22884837887898257 1106 4 -0.03830229767042681 1106 5 -0.0418520291274902 1106 6 -0.11763515013117853 1106 7 -0.033113090191093875 1106 8 0.0880932391696021 1106 9 0.05150349149469946 1106 10-11 0.015114957437766918 1106 12-13 0.07898077407312343 1106 14-15 -0.13426111798493423 1106 16-17 -0.12693098301017613 1106 18-19 -0.0047290574505467475 1106 20-21 -0.020370357235790948 1106 22-23 -0.31878652202787805 1106 24-25 -0.19504076845161933 1106 26-27 -0.1302744389745456 1106 28-29 -0.1781220136372852 1106 30-31 0.01854719908094893 1106 32-33 -0.28342717185115873 1106 34-35 -0.06780900939009626 1106 36-37 -0.33170637192776553 1106 38-39 -0.6385733379520175 1106 40-41 -0.6410206965249685 1106 42-43 -0.4439953271378201 1106 44-45 -0.5785133608987891 1106 46-47 -0.5079042914596812 1106 48-49 -0.18961807078044046 1106 50-51 -0.232486001703478 1106 52-53 -0.30047834323930545 1106 54-55 -0.2532740908290876 1106 56-57 -0.043723045832471996 1106 58-59 0.006378833035000753 1106 60-61 -0.020579512753421625 1106 62-63 -0.12857148487537984 1106 64-65 -0.3217302778489639 1106 66-67 -0.2827069235628912 1106 68-69 -0.15853177507485938 1106 70-71 -0.0942577685000856 1106 72-73 -0.19268939070147795 1106 74-75 -0.13987407864106416 1106 76-77 -0.03549358021085425 1106 78-79 -0.03063613073096505 1106 80-81 -0.13054321511112832 1106 82-83 -0.13719661562928565 1106 84-85 0.2755350690836167 1106 86-87 0.2730672227255191 1106 88-89 0.35651038289678283 1106 90-91 0.43666718412792704 1106 92-93 0.4329097619759388 1106 94-95 0.27349661636992906 1106 96-97 0.4862882773607673 1106 98-99 0.39827089067346577 1106 100-101 0.2619768644849252 1107 1 0.20081782250039737 1107 2 0.5080936667975067 1107 3 0.3888004526186002 1107 4 0.4941347568898209 1107 5 0.32936408731592337 1107 6 0.4479546761379041 1107 7 0.4629429316269764 1107 8 0.5615476668219017 1107 9 0.7437451176040639 1107 10-11 0.5652019735753058 1107 12-13 0.6245700729716717 1107 14-15 0.6566760194478221 1107 16-17 0.7889929701593275 1107 18-19 0.8416328392752348 1107 20-21 0.819218595104978 1107 22-23 0.6820101841664652 1107 24-25 0.6599455199145865 1107 26-27 0.5948242546533109 1107 28-29 0.7639896533056216 1107 30-31 0.8609764184218847 1107 32-33 0.6956362180866122 1107 34-35 0.6412406689138805 1107 36-37 0.8453413325036578 1107 38-39 0.8223673403971645 1107 40-41 0.7085769950988805 1107 42-43 0.7375726961267617 1107 44-45 0.8417067786283923 1107 46-47 0.9519270253091889 1107 48-49 0.44225093037977103 1107 50-51 0.23498653735961028 1107 52-53 0.3595899135750713 1107 54-55 0.4411561229820009 1107 56-57 0.398738945820007 1107 58-59 0.5653897119356586 1107 60-61 0.6058556884760975 1107 62-63 0.7520092254173889 1107 64-65 0.8037820249521204 1107 66-67 0.7195381681242417 1107 68-69 0.7295733826089297 1107 70-71 0.914385457802144 1107 72-73 0.9446228277203268 1107 74-75 0.9102976934745932 1107 76-77 0.9234204885281265 1107 78-79 1.0795654754889945 1107 80-81 1.1769834924393088 1107 82-83 1.4036199799679245 1107 84-85 1.2588494421907335 1107 86-87 1.3231297538027107 1107 88-89 1.3355662389698324 1107 90-91 1.3574316453812614 1107 92-93 1.5183080020651403 1107 94-95 1.556918723742566 1107 96-97 1.4496021230973533 1107 98-99 1.5444843702642252 1107 100-101 1.5084999859157548 1108 1 -0.07163218700766905 1108 2 0.2338078689243872 1108 3 0.22960615160782183 1108 4 0.08927028341732779 1108 5 0.21938500506382752 1108 6 0.16462158660116444 1108 7 0.156750254301528 1108 8 0.15057608862901617 1108 9 0.19097168753224025 1108 10-11 -0.00846360575854277 1108 12-13 0.2713892587885596 1108 14-15 0.3372141029140252 1108 16-17 0.2570970615537931 1108 18-19 0.3892737415176697 1108 20-21 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2543.0 13 3631.0 14 4210.0 15 5790.0 16 8192.0 17 11774.0 18 9833.0 19 4429.0 20 3846.0 21 3731.0 22 3698.0 23 3948.0 24 4301.0 25 4680.0 26 5400.0 27 6053.0 28 7029.0 29 8326.0 30 10001.0 31 12477.0 32 15923.0 33 20924.0 34 29278.0 35 44241.0 36 63180.0 37 96374.0 38 98711.0 39 20437.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28513756096058 19.548024992873486 16.17679915560452 18.990038290561415 2 14.97336399459191 21.784323468574136 41.747268027934105 21.495044508899856 3 17.839740849019904 29.459935212254972 32.43142783407866 20.268896104646462 4 11.192235673059882 18.036687138310743 40.04993334514884 30.72114384348054 5 10.995815835069509 38.95918174617094 34.53950094533989 15.505501473419663 6 26.16021311374291 38.8670429311621 18.934855354058723 16.03788860103627 7 23.58012395078187 36.92573548818384 22.762904154874903 16.731236406159393 8 25.629152599855093 32.33478240762152 21.250712975381152 20.78535201714224 9 27.413587145004048 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19.187671024481173 34-35 18.916092904671906 25.464441519603547 36.69981022767864 18.919655348045904 36-37 18.945573515422506 25.196030856566264 36.621760580467004 19.236635047544222 38-39 18.77138034851713 25.62300879332274 36.41723849420727 19.188372363952865 40-41 18.687055416267096 25.920555510623792 36.23229849067794 19.16009058243117 42-43 18.623403431815248 25.99568279105055 35.43500405340091 19.945909723733287 44-45 18.817594076898267 26.004265172412133 35.0664567778211 20.111683972868498 46-47 19.511096134405236 26.391662253887255 34.36980696095894 19.727434650748567 48-49 19.447231149305548 26.85753401972923 33.15862138019875 20.536613450766467 50-51 19.214827694769465 27.3593343673669 32.95717233218858 20.468665605675053 52-53 19.60195297418404 27.89650892866599 31.807367653841006 20.694170443308966 54-55 19.22134793811455 27.508656182960316 32.252706621835536 21.017289257089597 56-57 19.644966767988276 27.539675738753928 31.77852643011264 21.036831063145158 58-59 19.946005484179068 27.115009859198324 32.122211695474014 20.816772961148597 60-61 19.899870023588313 27.2311173157464 32.669522938429694 20.199489722235594 62-63 19.82526124637606 27.228068351083547 32.7855656623136 20.16110474022679 64-65 19.680756832947573 27.469662367471432 32.59297699693061 20.25660380265038 66-67 19.743798135348076 27.401447117801244 31.980456013512082 20.8742987333386 68-69 19.688886620159863 27.381964824212563 32.290317267842404 20.638831287785173 70-71 20.05249647214075 27.45180197752766 31.855918856829117 20.63978269350248 72-73 19.80745956968687 27.49973763742744 31.958938572497274 20.733864220388412 74-75 19.37278841478386 28.237520039670123 31.521281768594168 20.86840977695185 76-77 19.307574237309026 27.7671320200213 31.782850936067035 21.142442806602638 78-79 19.288771104014916 27.63099608254015 31.852376861949022 21.227855951495915 80-81 19.38569354430026 27.91278200994074 31.262073065106154 21.439451380652844 82-83 19.484185224134144 27.682887838550997 31.44956259461708 21.383364342697774 84-85 19.17060479662303 27.66752939635882 31.07425252560266 22.087613281415493 86-87 19.26425623913598 27.762289280194143 31.038275046826623 21.93517943384325 88-89 19.300009727973126 27.289854109298112 30.996693452303553 22.41344271042521 90-91 19.0058000774371 27.210472886419808 31.39795157676964 22.385775459373452 92-93 19.28834027290178 27.501206358012386 31.279683390015308 21.93076997907053 94-95 18.957636657573975 27.32159724542469 31.234696402625552 22.486069694375786 96-97 18.657313567931133 27.639754842250714 31.374029262937945 22.32890232688021 98-99 18.760943137100053 27.86544058586835 30.932311941767583 22.44130433526401 100-101 18.466548481578542 27.841066748882383 31.154327886542315 22.538056882996763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1038.0 1 742.5 2 561.5 3 655.5 4 526.5 5 511.5 6 921.0 7 2128.5 8 3731.0 9 4140.0 10 3112.0 11 2237.0 12 2114.0 13 2176.5 14 2260.0 15 2329.0 16 2339.0 17 2334.0 18 2436.5 19 2551.5 20 2676.5 21 2876.0 22 3140.0 23 3517.0 24 3834.0 25 4260.0 26 4895.0 27 5577.5 28 6344.5 29 7134.0 30 8098.0 31 9131.0 32 10432.0 33 11863.0 34 12952.5 35 13451.5 36 14843.5 37 16596.5 38 16924.5 39 16869.5 40 16800.0 41 16679.0 42 16283.5 43 16343.0 44 16859.0 45 17322.5 46 17357.5 47 18086.5 48 20774.0 49 19922.0 50 15793.5 51 13474.5 52 12179.5 53 11656.5 54 11084.5 55 9788.5 56 8714.0 57 7742.5 58 7167.0 59 6672.5 60 5950.0 61 5243.0 62 4205.5 63 2886.5 64 1882.0 65 1384.0 66 937.5 67 645.5 68 491.0 69 351.0 70 231.5 71 167.5 72 136.5 73 120.5 74 88.5 75 67.5 76 52.0 77 17.5 78 8.5 79 6.5 80 4.0 81 3.0 82 3.0 83 1.5 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027535425383811096 2 0.020988541026821045 3 0.018677875959648087 4 0.04602074592119477 5 0.09050104846427423 6 0.10532781597863405 7 0.04890907725516097 8 0.07297850503821263 9 0.059884736324232524 10-11 0.06450606645857844 12-13 0.06229167910253769 14-15 0.05266390798931703 16-17 0.04428774712081505 18-19 0.0552634061898866 20-21 0.052856463411581435 22-23 0.02830564707286875 24-25 0.005969218090196811 26-27 0.0018292765115119258 28-29 0.004813885556610331 30-31 0.004139941578684885 32-33 0.00654688435699005 34-35 0.0047176078454781245 36-37 0.004813885556610331 38-39 0.0031771644673628187 40-41 0.004813885556610331 42-43 0.0026957759117017853 44-45 0.0016367210892475124 46-47 0.0011553325335864795 48-49 0.0 50-51 0.001059054822454273 52-53 0.003947386156420472 54-55 0.009435215690956249 56-57 0.005295274112271365 58-59 0.0036585530230238516 60-61 0.0011553325335864795 62-63 0.00799105002397315 64-65 0.0017329988003797194 66-67 0.01771509884832602 68-69 0.03764458505269279 70-71 0.047850022432706696 72-73 0.0022143873560407524 74-75 0.009338937979824043 76-77 0.016174655470210714 78-79 0.02252898440493635 80-81 0.06845345261499891 82-83 0.02560987116116696 84-85 0.009435215690956249 86-87 0.024743371760977104 88-89 0.04034036096439458 90-91 0.03851108445288265 92-93 0.03976269469760134 94-95 0.009531493402088457 96-97 0.014152823536434374 98-99 0.03273442178495025 100-101 0.07143806166009732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 519331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.75426663565531 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23220587106746 42.83547059759906 2 12.227045816231005 13.389658535765726 3 3.998086676442223 6.567369117431355 4 1.8159684509840555 3.977280830684756 5 0.9605830414894196 2.6298009989700213 6 0.5945262774656179 1.9531710190953617 7 0.39664872594754225 1.520274707085729 8 0.2732253835499173 1.196820440201709 9 0.19567497560208394 0.9642635809237665 >10 1.1958678363545856 12.054100554274015 >50 0.07157342936462857 2.6525916800710267 >100 0.032979913246648326 3.567973996340547 >500 0.0021051008455307443 0.7734164275705079 >1k 0.0028068011273743258 2.7255917223970108 >5k 7.017002818435814E-4 3.1922157915894505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9741 1.8756823682776493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6876 1.3240110834901055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4808 0.9258064702472989 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.30000134788795585 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1464 0.281901138195101 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1448 0.2788202514388704 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1327 0.2555210453448764 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1227 0.23626550311843506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1180 0.22721539827200765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1176 0.22644517658295 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 824 0.15866566794587653 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.1394101257194352 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 649 0.12496846904960421 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 638 0.12285035940469566 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 603 0.11611091962544118 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.113222588291475 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.627771113220663E-5 0.0 18-19 0.0 0.0 0.0 1.9255542226441326E-4 0.0 20-21 0.0 0.0 0.0 1.9255542226441326E-4 0.0 22-23 0.0 0.0 0.0 2.8883313339661987E-4 0.0 24-25 0.0 0.0 0.0 0.0014441656669830995 0.0 26-27 0.0 0.0 0.0 0.0033697198896272323 0.0 28-29 0.0 0.0 0.0 0.0077022168905765305 0.0 30-31 0.0 0.0 0.0 0.014730489803227614 0.0 32-33 0.0 0.0 0.0 0.022047595849275316 0.0 34-35 0.0 0.0 0.0 0.026765203694753444 0.0 36-37 0.0 0.0 0.0 0.0319642000958926 0.0 38-39 0.0 0.0 0.0 0.040051527830997954 0.0 40-41 0.0 0.0 0.0 0.04823513327723552 0.0 42-43 0.0 0.0 0.0 0.06941622972632099 0.0 44-45 0.0 0.0 0.0 0.10224692922240344 0.0 46-47 0.0 0.0 0.0 0.13007118773961115 0.0 48-49 0.0 0.0 0.0 0.19602141986517269 0.0 50-51 0.0 0.0 0.0 0.264186039346775 0.0 52-53 0.0 0.0 0.0 0.3282107172496924 0.0 54-55 0.0 0.0 0.0 0.4309390350277569 0.0 56-57 0.0 0.0 0.0 0.544739289586025 0.0 58-59 0.0 0.0 0.0 0.6839568598831959 0.0 60-61 0.0 0.0 0.0 0.8403118627618995 0.0 62-63 0.0 0.0 0.0 1.0798508080588296 0.0 64-65 0.0 0.0 0.0 1.2404420302273502 0.0 66-67 0.0 0.0 0.0 1.4795958646797513 0.0 68-69 0.0 0.0 0.0 1.7874919848805484 0.0 70-71 0.0 0.0 0.0 1.9683978040979646 0.0 72-73 0.0 0.0 0.0 2.2480845549370247 0.0 74-75 0.0 0.0 0.0 2.5793761589429476 0.0 76-77 0.0 0.0 0.0 2.8151602735057217 0.0 78-79 0.0 0.0 0.0 3.034192066331492 0.0 80-81 0.0 0.0 0.0 3.3690459456493063 0.0 82-83 0.0 0.0 0.0 3.774471387227029 0.0 84-85 0.0 0.0 0.0 4.326238949725704 0.0 86-87 0.0 0.0 0.0 5.041871176571396 0.0 88-89 0.0 0.0 0.0 5.8281712433881285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4150 0.0 39.175404 1 GTATCAA 6790 0.0 36.965763 1 ATCAACG 8605 0.0 29.171612 3 TATCAAC 8645 0.0 29.036638 2 TCAACGC 8675 0.0 28.881418 4 CAACGCA 8780 0.0 28.536024 5 GTCCTAC 100 4.9214592E-5 28.522446 1 AACGCAG 8890 0.0 28.287165 6 GTACATG 4315 0.0 28.20296 1 TACATGG 4430 0.0 27.580795 2 GGATAGG 70 0.008355255 27.164232 1 CACGCCG 55 9.443729E-5 25.921997 14-15 ACATGGG 4465 0.0 25.554487 3 ACGCAGA 9900 0.0 25.300386 7 CGCAGAG 9870 0.0 25.232826 8 GAGTACT 5835 0.0 24.886509 12-13 CATGGGG 1070 0.0 24.437473 4 GCAGAGT 10060 0.0 23.905855 9 ATAGGAC 140 1.764928E-5 23.770996 3 AGTACTT 5980 0.0 23.686928 12-13 >>END_MODULE