FastQCFastQC Report
Thu 26 May 2016
SRR937084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519331
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65741.2658593459662528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51590.993393423462108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36460.7020570695760507No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29910.57593326799286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18280.3519913118993474No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.255328489922612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.21874295969237348No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.17541798968288047No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8190.15770289083455444No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7720.14865278598812703No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6980.13440368474056044No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6900.13286324136244515No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.10070648584428812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG752.3828761E-950.6634335
GCGCGCG651.3254258E-436.5466849
GTATAGG1901.2732926E-1129.9980851
GGGCGCG804.4894317E-429.6884637
GGTATCA57250.028.3737351
GTATCAA79850.027.599991
GCGCCTA458.940244E-426.39228632-33
TAAGGTG2409.094947E-1225.7275265
CATATAG2059.549694E-1025.4861773
GTACATG50250.024.1029381
CGGGTCG1000.001652117423.750776
TGGGCGC1000.001652117423.750776
TAGGATA2202.1609594E-923.750777
ATATAGG2402.2919266E-1023.7484864
GTATAGA1000.001653043723.7484841
GAGTACT49100.023.7058212-13
TACATGG51500.023.333462
GTATTGG1852.5376357E-723.1066321
GTAAGGT1652.4269684E-623.0288314
ATCAACG96300.022.7867073
Produced by FastQC (version 0.11.5)