##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937084_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 519331 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13491395660956 33.0 31.0 34.0 31.0 34.0 2 32.23246060797449 33.0 31.0 34.0 30.0 34.0 3 32.328243451671476 34.0 31.0 34.0 30.0 34.0 4 36.0297709938363 37.0 35.0 37.0 35.0 37.0 5 35.89293533411254 37.0 35.0 37.0 35.0 37.0 6 36.014326123416474 37.0 35.0 37.0 35.0 37.0 7 35.971476765299975 37.0 35.0 37.0 35.0 37.0 8 35.93934889309516 37.0 35.0 37.0 35.0 37.0 9 37.59971386264252 39.0 37.0 39.0 35.0 39.0 10-11 37.519808176288336 39.0 37.0 39.0 35.0 39.0 12-13 37.418181275525626 39.0 37.0 39.0 34.5 39.0 14-15 38.49661776400792 40.0 38.0 41.0 34.0 41.0 16-17 38.524778994514094 40.0 38.0 41.0 34.0 41.0 18-19 38.73475298027655 40.0 38.0 41.0 34.5 41.0 20-21 38.77752531622414 40.0 38.0 41.0 35.0 41.0 22-23 38.66772636334053 40.0 38.0 41.0 34.0 41.0 24-25 38.5555195049015 40.0 38.0 41.0 34.0 41.0 26-27 38.32593374938142 40.0 38.0 41.0 34.0 41.0 28-29 38.151142527598005 40.0 37.5 41.0 34.0 41.0 30-31 37.97817288010921 40.0 37.0 41.0 34.0 41.0 32-33 37.693892719672036 40.0 36.0 41.0 33.0 41.0 34-35 37.489471069510586 40.0 36.0 41.0 33.0 41.0 36-37 37.26627237734701 40.0 35.0 41.0 33.0 41.0 38-39 37.044416759253735 39.0 35.0 41.0 32.5 41.0 40-41 36.637830208479755 39.0 35.0 41.0 31.5 41.0 42-43 36.309737912814754 39.0 35.0 41.0 30.5 41.0 44-45 35.86847694437651 39.0 35.0 41.0 29.5 41.0 46-47 35.21056031702325 39.0 35.0 40.0 26.0 41.0 48-49 34.58801900907129 38.5 35.0 40.0 23.0 41.0 50-51 34.21368452874949 38.0 34.0 40.0 20.5 41.0 52-53 33.95354022771605 38.0 34.0 40.0 19.0 41.0 54-55 33.562701822151965 38.0 33.5 40.0 15.0 41.0 56-57 33.33234584494282 38.0 33.0 40.0 13.5 41.0 58-59 33.00579880654149 37.0 33.0 40.0 9.0 41.0 60-61 32.5971278433215 37.0 32.5 40.0 7.5 41.0 62-63 32.23651101128182 36.0 32.0 39.0 4.0 40.5 64-65 31.912709813201985 36.0 32.0 39.0 2.0 40.0 66-67 31.572557771440565 35.5 31.0 39.0 2.0 40.0 68-69 31.132919082434903 35.0 31.0 38.0 2.0 40.0 70-71 30.67269333045784 35.0 30.5 38.0 2.0 40.0 72-73 30.258470031636854 35.0 30.0 37.0 2.0 39.0 74-75 29.794620386612777 34.0 29.5 36.5 2.0 39.0 76-77 28.38790289815166 32.5 28.0 35.0 2.0 37.5 78-79 29.024582587983385 34.0 29.0 35.5 2.0 38.0 80-81 28.854260577550733 34.0 29.0 35.0 2.0 37.0 82-83 28.510095680789323 34.0 29.0 35.0 2.0 37.0 84-85 28.065723979504398 34.0 28.0 35.0 2.0 36.0 86-87 27.704930959253346 34.0 27.0 35.0 2.0 36.0 88-89 27.322348367418854 33.0 26.5 35.0 2.0 35.5 90-91 26.864396694978733 33.0 25.5 35.0 2.0 35.0 92-93 26.406532635255743 33.0 25.0 35.0 2.0 35.0 94-95 25.87579212486834 32.5 24.0 34.0 2.0 35.0 96-97 25.140021489185123 32.0 19.5 34.0 2.0 35.0 98-99 24.378446501364255 32.0 9.5 34.0 2.0 35.0 100-101 21.482204990651432 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11989552101839962 1101 2 0.2454301506774499 1101 3 0.09748425835208963 1101 4 0.09002618763945947 1101 5 0.019977875061115924 1101 6 0.00833997262091657 1101 7 0.19757380819952175 1101 8 0.18008629084991412 1101 9 0.19025254784281742 1101 10-11 0.17508049387197389 1101 12-13 0.2583231668824837 1101 14-15 0.40007635254201546 1101 16-17 0.3252328339338888 1101 18-19 0.12374041557092852 1101 20-21 -0.023051170529925002 1101 22-23 0.06948074154130524 1101 24-25 0.10741978004939057 1101 26-27 0.17646566609606396 1101 28-29 0.22086618871617958 1101 30-31 0.3223340932870755 1101 32-33 0.1527753354439838 1101 34-35 0.10192156439422462 1101 36-37 0.09709710515127057 1101 38-39 -0.021643775422568012 1101 40-41 0.32331121802540963 1101 42-43 0.2581538282645113 1101 44-45 -0.1803459868381978 1101 46-47 -0.14420964555210247 1101 48-49 -0.0483778380624571 1101 50-51 0.07868500110259902 1101 52-53 0.12808524522682063 1101 54-55 -0.12975726931674814 1101 56-57 -0.04664708716749999 1101 58-59 0.13555041629484066 1101 60-61 0.03183360267941282 1101 62-63 0.05707745546079224 1101 64-65 0.2238064623304794 1101 66-67 0.1600909758632234 1101 68-69 -0.11153112171366786 1101 70-71 -0.07354242007693657 1101 72-73 0.07382443395038862 1101 74-75 0.2784667865904389 1101 76-77 0.41617351438190653 1101 78-79 0.3173787104333812 1101 80-81 0.14298564280898418 1101 82-83 0.3100786068593102 1101 84-85 0.09318656024633398 1101 86-87 -0.46915376381684126 1101 88-89 -0.6918759778327761 1101 90-91 -0.5103468739707822 1101 92-93 -0.7411851522222577 1101 94-95 -0.9385216299842938 1101 96-97 -0.7590626392538589 1101 98-99 -0.4946122104703967 1101 100-101 -0.39339271143762744 1102 1 -0.1394624028962177 1102 2 -0.08443068463652992 1102 3 -0.04612644712508995 1102 4 0.05118716287169178 1102 5 -0.1359932940403752 1102 6 0.015274785380974265 1102 7 0.04386740026959757 1102 8 0.13284606119660936 1102 9 0.13993900469320408 1102 10-11 0.20439611644707867 1102 12-13 0.1272959388320345 1102 14-15 0.08229695901735568 1102 16-17 0.0657863154950249 1102 18-19 0.02227219045528983 1102 20-21 0.14922750100028281 1102 22-23 0.0071387096513433335 1102 24-25 -0.06386602181252243 1102 26-27 0.2549084798581518 1102 28-29 0.27545772722628925 1102 30-31 0.11408298992883203 1102 32-33 -0.13342876597332776 1102 34-35 0.08698778625527126 1102 36-37 0.05387519725291412 1102 38-39 0.15089864302464662 1102 40-41 0.19270761436676764 1102 42-43 -0.013180649669337186 1102 44-45 0.00952022524690932 1102 46-47 -0.20425331145094816 1102 48-49 -0.4099819895815031 1102 50-51 -0.5687515621630581 1102 52-53 -0.6026668306345329 1102 54-55 -0.1621360221095145 1102 56-57 -0.019665472058768785 1102 58-59 -0.01497269872936613 1102 60-61 0.07216651442501387 1102 62-63 0.0617485253771477 1102 64-65 0.19051426944288608 1102 66-67 0.07364003177145761 1102 68-69 0.48318253467399686 1102 70-71 0.12064964877832196 1102 72-73 -0.01524666773779515 1102 74-75 0.16743137118395168 1102 76-77 0.07535139793000312 1102 78-79 -0.27834207221268414 1102 80-81 -0.07960657187082276 1102 82-83 0.07375424092115779 1102 84-85 0.0222355845864044 1102 86-87 -0.25915113653094934 1102 88-89 -0.15020192828908918 1102 90-91 -0.2541793712569351 1102 92-93 -0.003765065501454501 1102 94-95 0.09284999655007198 1102 96-97 0.36980369706725114 1102 98-99 0.3196040538549276 1102 100-101 0.33359969794646815 1103 1 0.1508075233042092 1103 2 0.29342818234147217 1103 3 0.3040087365545503 1103 4 0.1500445614209056 1103 5 0.16687486878505808 1103 6 0.04531770383591294 1103 7 0.08588463067426488 1103 8 0.12508796750707774 1103 9 0.21365034814798634 1103 10-11 0.3020907462832838 1103 12-13 0.31111108066460247 1103 14-15 0.39718039854393083 1103 16-17 0.4153605971938603 1103 18-19 0.3176532571774189 1103 20-21 0.15874707900032092 1103 22-23 0.2342157801044138 1103 24-25 0.26574582141222436 1103 26-27 0.12547246995098504 1103 28-29 0.08706398664030246 1103 30-31 0.1346725042129222 1103 32-33 0.01812190742416675 1103 34-35 0.24395526024265735 1103 36-37 0.1799457952752661 1103 38-39 0.16140452619317358 1103 40-41 0.17475244871161522 1103 42-43 0.06344234227005074 1103 44-45 0.3777933403956695 1103 46-47 0.3333806905531773 1103 48-49 0.2756009388310261 1103 50-51 0.4915643075816405 1103 52-53 0.5133453453500394 1103 54-55 0.5888014404707533 1103 56-57 0.14391938484820344 1103 58-59 0.3307887099539002 1103 60-61 0.4126371679073273 1103 62-63 0.39659755451253176 1103 64-65 0.4929129131945018 1103 66-67 0.6375513462357993 1103 68-69 0.32409119588290025 1103 70-71 0.3063712271186816 1103 72-73 0.4283858343261997 1103 74-75 0.02537191557398799 1103 76-77 0.042847443255926265 1103 78-79 0.276746065025538 1103 80-81 0.26856230374128387 1103 82-83 0.2135259727217793 1103 84-85 0.15652749310266145 1103 86-87 0.036327615367607535 1103 88-89 0.44046882224817807 1103 90-91 0.30907020491543946 1103 92-93 0.2452249958196262 1103 94-95 0.604146814534225 1103 96-97 0.7543020049079026 1103 98-99 0.2420325703637225 1103 100-101 0.8807060109297744 1104 1 -0.011936995638350822 1104 2 0.11292141362564934 1104 3 -0.04974699480817435 1104 4 0.08178767069435366 1104 5 -0.08251926674007137 1104 6 0.1895061177382189 1104 7 0.12738294347000334 1104 8 0.19280654476762749 1104 9 0.08373666681698921 1104 10-11 0.2514578614048659 1104 12-13 0.27956318857027185 1104 14-15 0.4424081554105683 1104 16-17 0.12351405632917078 1104 18-19 0.2479060550459522 1104 20-21 0.16787955647808417 1104 22-23 0.21467521074890072 1104 24-25 0.2620488437176718 1104 26-27 0.07986768419765156 1104 28-29 0.2794942938978977 1104 30-31 0.10885964040623008 1104 32-33 0.027614656949218386 1104 34-35 0.06123868332981175 1104 36-37 0.010622122259853484 1104 38-39 0.2815060307478916 1104 40-41 0.5314825190693995 1104 42-43 0.6303136518380015 1104 44-45 0.5803552516807713 1104 46-47 0.1889964310902741 1104 48-49 0.3149606599972188 1104 50-51 0.39206847898734765 1104 52-53 0.3114526603388228 1104 54-55 0.5505539545203177 1104 56-57 0.41580643537216844 1104 58-59 0.24795022703435876 1104 60-61 0.4700010657157421 1104 62-63 0.6181271607058605 1104 64-65 0.3857927226692368 1104 66-67 0.49932313065067646 1104 68-69 0.2749988929276199 1104 70-71 0.2837892914104856 1104 72-73 0.5916508625928927 1104 74-75 0.6638849974290544 1104 76-77 0.8478062109579589 1104 78-79 0.7428888710001864 1104 80-81 0.6185990640577366 1104 82-83 0.7329701902506862 1104 84-85 0.7377667451611352 1104 86-87 0.7166971874607704 1104 88-89 0.7232938996961948 1104 90-91 0.7190126661181218 1104 92-93 0.7592159094234603 1104 94-95 0.9472336757059061 1104 96-97 0.9393928165044692 1104 98-99 0.45605720710686626 1104 100-101 0.8423726461773668 1105 1 -0.0010682098013106156 1105 2 0.16360155120288056 1105 3 0.10924166918003664 1105 4 0.09920687183049637 1105 5 -0.057867315377826856 1105 6 0.08794461160530176 1105 7 0.08970186450187612 1105 8 0.11567031602120181 1105 9 0.07401281585744357 1105 10-11 0.2937545313791219 1105 12-13 0.1747449787858244 1105 14-15 0.2885485683735425 1105 16-17 0.058245829312866704 1105 18-19 0.21486254058160625 1105 20-21 0.11408612123398143 1105 22-23 0.07121718359891105 1105 24-25 -0.14822064388349077 1105 26-27 0.04522241740469468 1105 28-29 0.1930447716479975 1105 30-31 0.24776568404328714 1105 32-33 0.24599652858235288 1105 34-35 0.22376392393960742 1105 36-37 0.11786319928506117 1105 38-39 0.27267384119294036 1105 40-41 0.37959823382101376 1105 42-43 0.5742465378371833 1105 44-45 0.33554273187571226 1105 46-47 0.15964909023043816 1105 48-49 0.07933884470875086 1105 50-51 0.1899180831174334 1105 52-53 0.40958167483901775 1105 54-55 0.7131971581175023 1105 56-57 0.3922531588923661 1105 58-59 0.22857769760721425 1105 60-61 0.45478268384410825 1105 62-63 0.6483858234681819 1105 64-65 0.7355310067472374 1105 66-67 0.6403108740649905 1105 68-69 0.5567153237839619 1105 70-71 0.07454376879975655 1105 72-73 0.3453152324508011 1105 74-75 0.3714316217215625 1105 76-77 0.43541570258374307 1105 78-79 0.6197343556777213 1105 80-81 0.35485348477851275 1105 82-83 0.5099819796104264 1105 84-85 0.22130859542215475 1105 86-87 0.4057687262301144 1105 88-89 0.4543105324774146 1105 90-91 0.28995352502744964 1105 92-93 0.44049379552672363 1105 94-95 0.5035020206851932 1105 96-97 0.4887533877228911 1105 98-99 0.7260178167210789 1105 100-101 1.0764019611362308 1106 1 -0.0951749220796394 1106 2 0.010558992198731687 1106 3 0.04828358677022493 1106 4 -0.026934904411056948 1106 5 -0.0663422942943157 1106 6 -0.014800402588953432 1106 7 -0.15174001032738005 1106 8 -0.12373150606956784 1106 9 0.23443460492727297 1106 10-11 0.036320365395440035 1106 12-13 0.0429151079000718 1106 14-15 0.23038330925417938 1106 16-17 0.3409000359194323 1106 18-19 0.04334221132243243 1106 20-21 -0.02526848566240858 1106 22-23 -0.1276048121487534 1106 24-25 -0.06563117257488926 1106 26-27 -0.2705833885794604 1106 28-29 0.18590013279969497 1106 30-31 -0.013675834444875079 1106 32-33 -0.1803329206331057 1106 34-35 -0.1292631936173123 1106 36-37 0.09498016230872963 1106 38-39 0.29185538510009934 1106 40-41 0.4867811793364609 1106 42-43 0.48636391659212563 1106 44-45 0.33595994790155714 1106 46-47 -0.10307525618060964 1106 48-49 0.07804562350407451 1106 50-51 -0.02013608072819295 1106 52-53 -0.014507279775010318 1106 54-55 -0.005952612042499084 1106 56-57 -0.07198652800258998 1106 58-59 -0.08111685868873764 1106 60-61 -0.22052970851118658 1106 62-63 -0.09378209633131718 1106 64-65 -0.06550297631467927 1106 66-67 -0.06498019721510317 1106 68-69 -0.2590875126911101 1106 70-71 -0.1906586329391864 1106 72-73 0.08048550410149602 1106 74-75 -0.2588808300239158 1106 76-77 -0.31836002821244236 1106 78-79 -0.06744864922790939 1106 80-81 0.21664065412367606 1106 82-83 0.21949717755790132 1106 84-85 0.10357052987915338 1106 86-87 -0.106572553509519 1106 88-89 -0.03464734800578029 1106 90-91 -0.22676778060630198 1106 92-93 -0.4899350609747195 1106 94-95 -0.5354995115814916 1106 96-97 -0.6324293083537924 1106 98-99 -0.3767568925639786 1106 100-101 -0.1046490075176294 1107 1 0.2892216906947809 1107 2 0.3884651805152117 1107 3 0.326674955974795 1107 4 0.14154114543299556 1107 5 0.07328282059712166 1107 6 0.13952328237495948 1107 7 0.31693041322649407 1107 8 0.38390029889374233 1107 9 0.2885239866983369 1107 10-11 0.3476253061039287 1107 12-13 0.44720300700655713 1107 14-15 0.6314128118684366 1107 16-17 0.6059621149647398 1107 18-19 0.5446738219120348 1107 20-21 0.49034733243995277 1107 22-23 0.4399720709407333 1107 24-25 0.5421727811973156 1107 26-27 0.5441080243215239 1107 28-29 0.5181737024954671 1107 30-31 0.46006864699921124 1107 32-33 0.454082526803127 1107 34-35 0.6518855700754855 1107 36-37 0.759903994936387 1107 38-39 0.5083315705537075 1107 40-41 0.728024945063396 1107 42-43 0.5848126938458833 1107 44-45 0.5082989868817762 1107 46-47 0.460774247337703 1107 48-49 0.8047264287235407 1107 50-51 0.4335570197584957 1107 52-53 0.38877861532346003 1107 54-55 0.2791062274409839 1107 56-57 0.46854001380282284 1107 58-59 0.3548697762352475 1107 60-61 0.41763572009038086 1107 62-63 0.41124487490439066 1107 64-65 0.3796090522537412 1107 66-67 0.44942831906557856 1107 68-69 0.24017228509995903 1107 70-71 0.2767253832134209 1107 72-73 0.4857509221556455 1107 74-75 0.7814950344870617 1107 76-77 0.5324977022861468 1107 78-79 0.8998807464054615 1107 80-81 0.5174377370112566 1107 82-83 0.5542173698221298 1107 84-85 0.7228203076301405 1107 86-87 0.8943541118512748 1107 88-89 0.9472205230747228 1107 90-91 0.9666528953320395 1107 92-93 1.200530456526451 1107 94-95 1.308263201815226 1107 96-97 1.4636463015125578 1107 98-99 1.7014220216554357 1107 100-101 2.113629655070703 1108 1 -0.018367941123599962 1108 2 0.16350065291987903 1108 3 0.07147111764119529 1108 4 0.003085401828997192 1108 5 -5.253797451700848E-4 1108 6 0.0499463182276898 1108 7 0.046177228023971395 1108 8 0.15314682113530154 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-1.5094989957750968 2316 66-67 -1.9578926253442503 2316 68-69 -1.8930437267556854 2316 70-71 -1.522204409144404 2316 72-73 -1.9088997236738265 2316 74-75 -1.8020586274586776 2316 76-77 -1.7993971799152284 2316 78-79 -2.089949754933347 2316 80-81 -2.0110793812588987 2316 82-83 -1.985721752119229 2316 84-85 -1.5660999698997067 2316 86-87 -1.787410678233094 2316 88-89 -2.5404523015661518 2316 90-91 -2.858678439606745 2316 92-93 -2.3774848177195196 2316 94-95 -2.196047941479769 2316 96-97 -2.2468145361291114 2316 98-99 -2.8652575559872453 2316 100-101 -3.2933376408657082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 2.0 5 17.0 6 49.0 7 104.0 8 216.0 9 441.0 10 778.0 11 1309.0 12 2109.0 13 2837.0 14 3781.0 15 5285.0 16 7507.0 17 10791.0 18 9163.0 19 4687.0 20 3673.0 21 3658.0 22 3696.0 23 4038.0 24 4343.0 25 4969.0 26 5865.0 27 7036.0 28 8384.0 29 10347.0 30 12755.0 31 15698.0 32 19689.0 33 25844.0 34 35890.0 35 50833.0 36 75208.0 37 106721.0 38 68173.0 39 3431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56398640996603 20.97210345066679 16.073258295390637 18.39065184397655 2 15.356353357108196 23.70059847953847 39.88015004351878 21.062898119834554 3 19.07330242159098 29.62308983917678 31.373428738677678 19.930179000554563 4 12.058590763886617 18.976721975002455 38.73194552221994 30.232741738890994 5 11.581172963719656 40.80648236048502 32.98312976467167 14.62921491112365 6 27.037477061835325 39.42707059659447 17.953675016511628 15.581777325058585 7 25.236603386292693 36.9977413161984 21.473670832940197 16.291984464568703 8 27.048837831748923 33.17883969953652 20.298037282580857 19.4742851861337 9 28.35109015252315 16.73345130562204 21.199774325045105 33.715684216809706 10-11 24.16204694116084 28.820251438870393 26.692995411404286 20.32470620856448 12-13 27.002331074152426 27.1649574369546 27.682011059362953 18.15070042953002 14-15 23.355696426783336 27.226905805417978 28.90134563688601 20.516052130912676 16-17 19.48612734460296 30.639418790713435 28.769994473659384 21.10445939102422 18-19 19.42406562301427 27.735447595940926 34.14871083896559 18.691775942079218 20-21 21.658808702458675 26.012252454871664 34.15841155192948 18.170527290740182 22-23 20.752691484875502 26.828678876152225 33.95324297210022 18.465386666872064 24-25 20.25532898157241 27.06275393295207 34.32509194539118 18.35682514008434 26-27 20.112625774915973 26.801237362334774 34.592807470387356 18.493329392361897 28-29 19.38667246900339 27.486516306555934 34.3852956977342 18.741515526706472 30-31 19.754934714084083 26.885550833668702 34.398485744159316 18.961028708087905 32-33 19.642963736037323 27.570470470663217 33.86173750459726 18.924828288702194 34-35 20.14678499839216 27.084845695712367 34.10734194569552 18.66102736019995 36-37 20.234208542459562 26.942358664685884 33.98758192148054 18.835850871374006 38-39 19.746773285062993 27.661985663324014 33.703898054583355 18.88734299702964 40-41 19.94011514836424 27.609997496774692 33.33824350605588 19.11164384880519 42-43 19.7845882491128 28.13773874465418 32.57585239471551 19.50182061151751 44-45 20.04138039928523 27.972591502865242 32.30087728757163 19.6851508102779 46-47 20.73373384938286 28.653649895057093 31.4495600100129 19.163056245547146 48-49 20.72868748447522 29.093391305352462 30.341054163914727 19.836867046257588 50-51 20.239236845247035 29.47490747480349 30.44242641310334 19.843429266846133 52-53 20.81863485119231 29.81853472179432 29.548281625485238 19.81454880152813 54-55 20.26222197403968 29.528470281958906 29.74933135129619 20.459976392705233 56-57 20.570562619685575 29.719185988856783 29.561675198501142 20.148576192956494 58-59 20.92446036239 29.0427088748965 29.80465214795987 20.228178614753624 60-61 20.271605175999383 29.67399676500039 30.401005160594625 19.65339289840561 62-63 20.361965682772645 29.424105243091592 30.52946964460046 19.684459429535305 64-65 20.22785083116548 29.910885350576027 30.101900329462328 19.759363488796165 66-67 20.22890988598793 29.595287013484654 29.622533605735068 20.55326949479234 68-69 20.221342884100668 29.750351414322303 29.636550940635047 20.391754760941982 70-71 20.630543806966482 29.892582647432892 29.347360394508687 20.12951315109194 72-73 20.342364200026957 29.89284270117267 29.361292434482895 20.403500664317487 74-75 20.06250577687402 30.79016082817266 28.83805881628 20.30927457867332 76-77 20.262434037669657 30.059181966012012 29.027565298013503 20.65081869830484 78-79 20.27410317139391 30.237132459130034 28.70535112548861 20.783413243987443 80-81 20.22901057342183 30.60101555685212 28.50953456532665 20.660439304399397 82-83 20.309052047830857 30.224520054685843 28.72749504168833 20.738932855794967 84-85 19.85791020749269 30.63037301699628 28.284418359025608 21.22729841648542 86-87 19.933972554996394 30.324678263029544 28.08900707546943 21.652342106504637 88-89 19.970538254536002 30.029317325143097 28.102942804738912 21.89720161558199 90-91 19.94723920473692 29.784720550714873 28.22577384104366 22.04226640350455 92-93 20.028170858277285 30.474880182388496 28.032218373253283 21.46473058608094 94-95 19.893382062692194 30.232645461179864 27.93045283258654 21.9435196435414 96-97 19.796353189346668 30.468458910077494 27.944825116183242 21.7903627843926 98-99 19.66824530476316 30.98851499866655 27.29461738645696 22.048622310113327 100-101 19.082068859277516 31.05253023184164 27.223677116228917 22.64172379265193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1303.0 1 926.0 2 545.0 3 562.0 4 570.0 5 653.0 6 946.5 7 1575.0 8 2334.0 9 2642.5 10 2351.5 11 2049.0 12 2079.0 13 2164.0 14 2213.0 15 2277.0 16 2365.5 17 2411.5 18 2425.0 19 2523.0 20 2646.5 21 2817.5 22 3051.5 23 3348.0 24 3730.5 25 4204.5 26 4754.5 27 5453.0 28 6178.5 29 7054.5 30 8178.5 31 9073.0 32 10246.0 33 11660.5 34 12921.5 35 13520.0 36 14850.5 37 16347.5 38 16907.0 39 17087.5 40 16706.5 41 16568.0 42 16322.5 43 16330.5 44 16927.0 45 17351.5 46 17194.5 47 17809.0 48 20676.0 49 19983.5 50 15771.5 51 13566.0 52 12267.5 53 11692.0 54 11109.5 55 10060.5 56 9131.0 57 8273.5 58 7720.5 59 7378.0 60 6845.0 61 5934.5 62 4885.0 63 3422.5 64 2068.5 65 1417.5 66 973.0 67 730.5 68 543.0 69 370.0 70 256.0 71 188.5 72 160.5 73 133.5 74 94.0 75 73.5 76 53.0 77 23.0 78 11.5 79 8.5 80 2.5 81 1.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024454538627580485 2 0.002888331333966199 3 5.776662667932397E-4 4 0.0 5 0.0026957759117017853 6 0.0 7 3.851108445288265E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.008183605446237563 14-15 0.004428774712081505 16-17 0.0 18-19 1.9255542226441326E-4 20-21 0.001251610244718686 22-23 0.001540443378115306 24-25 1.9255542226441326E-4 26-27 9.627771113220663E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0014441656669830995 38-39 0.006450606645857844 40-41 1.9255542226441326E-4 42-43 0.0 44-45 5.776662667932397E-4 46-47 1.9255542226441326E-4 48-49 0.0 50-51 0.002503220489437372 52-53 5.776662667932397E-4 54-55 0.0 56-57 2.8883313339661987E-4 58-59 1.9255542226441326E-4 60-61 0.002118109644908546 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.9255542226441326E-4 70-71 2.8883313339661987E-4 72-73 1.9255542226441326E-4 74-75 0.0036585530230238516 76-77 9.627771113220663E-5 78-79 1.9255542226441326E-4 80-81 0.0019255542226441326 82-83 1.9255542226441326E-4 84-85 0.0022143873560407524 86-87 2.8883313339661987E-4 88-89 0.002599498200569579 90-91 0.0011553325335864795 92-93 0.0 94-95 0.0 96-97 9.627771113220663E-5 98-99 2.8883313339661987E-4 100-101 0.002118109644908546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 519331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.52115856329812 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02977410875252 41.2272275416958 2 12.531668298074603 13.41418812087814 3 4.344075645711632 6.974998843352817 4 1.9526801965065774 4.180388256825626 5 1.0848509244919422 2.9031239173636902 6 0.65393262992184 2.099953918545681 7 0.45867534202818055 1.7184184996835674 8 0.3090443958416518 1.3232331290351773 9 0.2266539781939453 1.0917705155328412 >10 1.272934328937097 12.394650861086948 >50 0.08817531250491197 3.212970668708684 >100 0.04281764126829553 4.201293171446772 >500 0.0021771682000828233 0.8570381715709612 >1k 0.0018143068334023527 2.1221065263439596 >5k 7.257227333609411E-4 2.27863785792932 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6574 1.2658593459662528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5159 0.993393423462108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3646 0.7020570695760507 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2991 0.57593326799286 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1828 0.3519913118993474 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1326 0.255328489922612 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1136 0.21874295969237348 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.17541798968288047 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 819 0.15770289083455444 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 772 0.14865278598812703 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 698 0.13440368474056044 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 690 0.13286324136244515 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.10070648584428812 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.627771113220663E-5 0.0 18-19 0.0 0.0 0.0 1.9255542226441326E-4 0.0 20-21 0.0 0.0 0.0 1.9255542226441326E-4 0.0 22-23 0.0 0.0 0.0 2.8883313339661987E-4 0.0 24-25 0.0 0.0 0.0 0.0014441656669830995 0.0 26-27 0.0 0.0 0.0 0.003465997600759439 0.0 28-29 0.0 0.0 0.0 0.0077022168905765305 0.0 30-31 0.0 0.0 0.0 0.01482676751435982 0.0 32-33 0.0 0.0 0.0 0.021758762715878698 0.0 34-35 0.0 0.0 0.0 0.026668925983621236 0.0 36-37 0.0 0.0 0.0 0.0319642000958926 0.0 38-39 0.0 0.0 0.0 0.04034036096439458 0.0 40-41 0.0 0.0 0.0 0.049197910388557586 0.0 42-43 0.0 0.0 0.0 0.07086039539330408 0.0 44-45 0.0 0.0 0.0 0.10397992802278316 0.0 46-47 1.9255542226441326E-4 0.0 0.0 0.13190046425112306 0.0 48-49 1.9255542226441326E-4 0.0 0.0 0.19852464035461007 0.0 50-51 1.9255542226441326E-4 0.0 0.0 0.2672669261030056 0.0 52-53 1.9255542226441326E-4 0.0 0.0 0.3310990485836586 0.0 54-55 1.9255542226441326E-4 0.0 0.0 0.435175254317574 0.0 56-57 1.9255542226441326E-4 0.0 0.0 0.5495531751426355 0.0 58-59 1.9255542226441326E-4 0.0 0.0 0.6895409671288639 0.0 60-61 1.9255542226441326E-4 0.0 0.0 0.8465699139854929 0.0 62-63 1.9255542226441326E-4 0.0 0.0 1.0879381357939348 0.0 64-65 1.9255542226441326E-4 0.0 0.0 1.249203301940381 0.0 66-67 1.9255542226441326E-4 0.0 0.0 1.4873943592814602 0.0 68-69 1.9255542226441326E-4 9.627771113220663E-5 0.0 1.796253256593579 0.0 70-71 1.9255542226441326E-4 1.9255542226441326E-4 0.0 1.9801436848560938 0.0 72-73 1.9255542226441326E-4 1.9255542226441326E-4 0.0 2.261370879073269 0.0 74-75 1.9255542226441326E-4 1.9255542226441326E-4 0.0 2.594010371035043 0.0 76-77 1.9255542226441326E-4 1.9255542226441326E-4 0.0 2.8308535404202715 0.0 78-79 1.9255542226441326E-4 1.9255542226441326E-4 0.0 3.0510406657796283 0.0 80-81 1.9255542226441326E-4 1.9255542226441326E-4 0.0 3.3871461553421613 0.0 82-83 1.9255542226441326E-4 1.9255542226441326E-4 0.0 3.794112040297999 0.0 84-85 1.9255542226441326E-4 1.9255542226441326E-4 0.0 4.347227490752526 0.0 86-87 1.9255542226441326E-4 1.9255542226441326E-4 0.0 5.062474606753689 0.0 88-89 1.9255542226441326E-4 1.9255542226441326E-4 0.0 5.846849119347777 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 75 2.3828761E-9 50.663433 5 GCGCGCG 65 1.3254258E-4 36.546684 9 GTATAGG 190 1.2732926E-11 29.998085 1 GGGCGCG 80 4.4894317E-4 29.688463 7 GGTATCA 5725 0.0 28.373735 1 GTATCAA 7985 0.0 27.59999 1 GCGCCTA 45 8.940244E-4 26.392286 32-33 TAAGGTG 240 9.094947E-12 25.727526 5 CATATAG 205 9.549694E-10 25.486177 3 GTACATG 5025 0.0 24.102938 1 CGGGTCG 100 0.0016521174 23.75077 6 TGGGCGC 100 0.0016521174 23.75077 6 TAGGATA 220 2.1609594E-9 23.75077 7 ATATAGG 240 2.2919266E-10 23.748486 4 GTATAGA 100 0.0016530437 23.748484 1 GAGTACT 4910 0.0 23.70582 12-13 TACATGG 5150 0.0 23.33346 2 GTATTGG 185 2.5376357E-7 23.106632 1 GTAAGGT 165 2.4269684E-6 23.028831 4 ATCAACG 9630 0.0 22.786707 3 >>END_MODULE