##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1104756 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19861308741478 33.0 31.0 34.0 31.0 34.0 2 32.17872543801527 33.0 31.0 34.0 30.0 34.0 3 32.355870436548884 34.0 31.0 34.0 31.0 34.0 4 36.060746445368935 37.0 35.0 37.0 35.0 37.0 5 36.007552798989096 37.0 35.0 37.0 35.0 37.0 6 36.07755830246679 37.0 35.0 37.0 35.0 37.0 7 36.02265296590378 37.0 35.0 37.0 35.0 37.0 8 36.00903004826405 37.0 35.0 37.0 35.0 37.0 9 37.685590302292994 39.0 37.0 39.0 35.0 39.0 10-11 37.614886454565536 39.0 37.0 39.0 35.0 39.0 12-13 37.54582459837286 39.0 37.0 39.0 35.0 39.0 14-15 38.68952239227485 40.0 38.0 41.0 34.5 41.0 16-17 38.64214541491515 40.0 38.0 41.0 34.5 41.0 18-19 38.76102505892704 40.0 38.0 41.0 34.5 41.0 20-21 38.849369906115015 40.0 38.0 41.0 35.0 41.0 22-23 38.77559841268117 40.0 38.0 41.0 35.0 41.0 24-25 38.64512661619398 40.0 38.0 41.0 34.0 41.0 26-27 38.466801266524016 40.0 38.0 41.0 34.0 41.0 28-29 38.3187233199005 40.0 38.0 41.0 34.0 41.0 30-31 38.17185604785129 40.0 37.5 41.0 34.0 41.0 32-33 38.113248536328385 40.0 37.0 41.0 34.0 41.0 34-35 37.987886012839034 40.0 37.0 41.0 33.0 41.0 36-37 37.791163388114654 40.0 37.0 41.0 33.0 41.0 38-39 37.62020075021091 40.0 37.0 41.0 33.0 41.0 40-41 37.479005318821535 40.0 37.0 41.0 33.0 41.0 42-43 37.21175671369968 40.0 36.0 41.0 32.5 41.0 44-45 36.80308276216649 40.0 36.0 41.0 31.0 41.0 46-47 36.4168105898497 39.5 36.0 41.0 30.5 41.0 48-49 36.00336680678811 39.0 35.5 41.0 29.5 41.0 50-51 35.71415181270797 39.0 35.0 41.0 28.0 41.0 52-53 35.51671726607505 39.0 35.0 40.0 28.0 41.0 54-55 35.29757792670961 39.0 35.0 40.0 27.0 41.0 56-57 35.008654852293176 38.0 34.5 40.0 26.5 41.0 58-59 34.69775678973457 38.0 34.0 40.0 26.0 41.0 60-61 34.30756112661982 38.0 34.0 40.0 26.0 41.0 62-63 34.01070326841402 37.0 34.0 40.0 25.0 41.0 64-65 33.63126427917115 37.0 33.0 39.5 23.5 41.0 66-67 33.21026362382281 36.0 33.0 39.0 22.5 40.0 68-69 32.75511425147272 35.5 32.5 39.0 22.0 40.0 70-71 32.23544112908189 35.0 32.0 38.0 20.5 40.0 72-73 31.892875440368734 35.0 31.5 37.0 19.5 39.5 74-75 31.47486775360351 35.0 31.0 37.0 18.0 39.0 76-77 30.076997092570668 33.5 29.5 35.5 17.5 37.5 78-79 30.793140747821237 34.0 31.0 36.0 17.5 38.0 80-81 30.58893773828791 34.0 31.0 35.5 13.5 37.0 82-83 30.259689017303366 34.0 30.5 35.0 9.0 37.0 84-85 29.88935249050469 34.0 30.0 35.0 7.0 36.5 86-87 29.63241566463545 34.0 30.0 35.0 2.0 36.0 88-89 29.28590385569302 34.0 30.0 35.0 2.0 36.0 90-91 28.87188935837416 34.0 29.0 35.0 2.0 35.0 92-93 28.461964904467592 34.0 29.0 35.0 2.0 35.0 94-95 28.00691148090619 34.0 29.0 35.0 2.0 35.0 96-97 27.41376195286561 33.0 27.0 35.0 2.0 35.0 98-99 26.81728046736112 33.0 26.5 35.0 2.0 35.0 100-101 23.94225059651181 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28225439378568495 1101 2 0.2248776468849627 1101 3 0.19711790091378845 1101 4 0.12962085772663556 1101 5 0.2512394212262521 1101 6 0.12218947469233399 1101 7 0.23123601105159963 1101 8 0.20313138875607706 1101 9 0.21863047507602573 1101 10-11 0.2915810470906379 1101 12-13 0.28537655265029827 1101 14-15 0.2783756806182396 1101 16-17 0.40279642557304385 1101 18-19 0.37733492493548226 1101 20-21 0.2952254786026387 1101 22-23 0.2073238683925922 1101 24-25 0.28051238630068553 1101 26-27 0.3515100651944323 1101 28-29 0.467139269318416 1101 30-31 0.3441502179765976 1101 32-33 0.41988806014637703 1101 34-35 0.4566334573958528 1101 36-37 0.42408151983217834 1101 38-39 0.3887023378191543 1101 40-41 0.29026726663154534 1101 42-43 0.4263395168004749 1101 44-45 0.6484387387128123 1101 46-47 0.6142407970949719 1101 48-49 0.3053541371395312 1101 50-51 0.364519061606714 1101 52-53 0.301829130288759 1101 54-55 0.35099265075940167 1101 56-57 0.3819881387915629 1101 58-59 0.449848749375775 1101 60-61 0.4441233427694584 1101 62-63 0.37651236800644483 1101 64-65 0.34217176184198905 1101 66-67 0.35327002965539833 1101 68-69 0.3519682720467472 1101 70-71 0.32818040842442286 1101 72-73 0.30549043280270993 1101 74-75 0.1869617943506121 1101 76-77 -0.012359541726961965 1101 78-79 -0.06211751069967164 1101 80-81 0.24467673177463922 1101 82-83 0.3933600848118033 1101 84-85 0.30656827811311516 1101 86-87 0.31532761859970293 1101 88-89 0.46271940083611796 1101 90-91 0.14543825512289388 1101 92-93 0.2927231199706384 1101 94-95 0.3584837639465732 1101 96-97 0.44192700747100133 1101 98-99 0.7453539344984605 1101 100-101 0.8581131968637123 1102 1 0.310324569224278 1102 2 0.1538250153060119 1102 3 0.21290737459799658 1102 4 0.1752348928143519 1102 5 0.2152745089455479 1102 6 0.2230666676747859 1102 7 0.19877987070072578 1102 8 0.26190331858063587 1102 9 0.23441994876024097 1102 10-11 0.32052841551168854 1102 12-13 0.3309905877380146 1102 14-15 0.4972353297410521 1102 16-17 0.3527964255730467 1102 18-19 0.4273349249354794 1102 20-21 0.39347109263771785 1102 22-23 0.30688527190136483 1102 24-25 0.5138457196340198 1102 26-27 0.489229363440046 1102 28-29 0.28336733949385007 1102 30-31 0.31915021797659904 1102 32-33 0.3865547268130385 1102 34-35 0.5123352117818172 1102 36-37 0.4161867829900743 1102 38-39 0.3242286536086283 1102 40-41 0.24202165259646335 1102 42-43 0.30967285013381485 1102 44-45 0.44580715976544383 1102 46-47 0.4743285163932214 1102 48-49 0.39702080380619265 1102 50-51 0.4825015177470675 1102 52-53 0.42507474432384384 1102 54-55 0.3009926507594045 1102 56-57 0.4113741037038423 1102 58-59 0.31081366165646784 1102 60-61 0.34061457083962665 1102 62-63 0.3852842978310065 1102 64-65 0.4219963232454944 1102 66-67 0.45370862614662855 1102 68-69 0.24670511415201446 1102 70-71 0.08563654877529814 1102 72-73 0.15636762578516894 1102 74-75 0.05011968908745601 1102 76-77 0.0968509845888228 1102 78-79 0.32779477000208246 1102 80-81 0.39116795984481456 1102 82-83 0.3723074532328532 1102 84-85 0.39867354127100896 1102 86-87 0.21225744316110706 1102 88-89 0.3210527341694487 1102 90-91 0.6085961498597392 1102 92-93 0.5795652252338002 1102 94-95 0.5733960446483302 1102 96-97 0.7879796390499436 1102 98-99 0.9050030573054784 1102 100-101 1.0265342494952883 1103 1 0.09365790255760942 1103 2 -0.22457895923210458 1103 3 -0.05188846455741469 1103 4 0.052590893435372266 1103 5 0.1235573842754647 1103 6 0.1443909961952059 1103 7 0.16722421164157453 1103 8 0.17676125989409996 1103 9 0.12589642756787356 1103 10-11 0.11257933928128949 1103 12-13 0.2274158322652582 1103 14-15 0.21991038019905318 1103 16-17 0.3078267003750952 1103 18-19 0.3273155180110834 1103 20-21 0.3165342815835359 1103 22-23 0.41478777151323243 1103 24-25 0.30028416086985743 1103 26-27 0.20315189099788 1103 28-29 0.32871744040986073 1103 30-31 0.4139491622399092 1103 32-33 0.4829760899554074 1103 34-35 0.33570114874812873 1103 36-37 0.4325274133269801 1103 38-39 0.4692876506587851 1103 40-41 0.4722925732609582 1103 42-43 0.4240805508014063 1103 44-45 0.5586467809422757 1103 46-47 0.5494294324000464 1103 48-49 0.560240800701088 1103 50-51 0.2221537456619842 1103 52-53 0.21514228042072148 1103 54-55 0.26925068522610474 1103 56-57 0.21432007482633963 1103 58-59 0.19855624821135365 1103 60-61 0.16464422462009765 1103 62-63 0.21334671049984877 1103 64-65 0.05715002608666708 1103 66-67 -0.019905719451877246 1103 68-69 0.19337100454170297 1103 70-71 0.01914842581302878 1103 72-73 -0.05541237731994286 1103 74-75 -0.02817560667407193 1103 76-77 0.03483421700615352 1103 78-79 -0.04260967030221963 1103 80-81 -0.02259543093301275 1103 82-83 0.0497760140153396 1103 84-85 0.07722500843449609 1103 86-87 0.004056076913631301 1103 88-89 0.061353153359018364 1103 90-91 0.22907433647672448 1103 92-93 0.11690802914623788 1103 94-95 0.39124653370282303 1103 96-97 0.41049167134306686 1103 98-99 0.28259970378894295 1103 100-101 0.6303185997514618 1104 1 0.31856565163510453 1104 2 0.1640276436480832 1104 3 0.16897004048781383 1104 4 0.18769685953927961 1104 5 0.17395904315931432 1104 6 0.22338064449876782 1104 7 0.2293294919864124 1104 8 0.19974561805626223 1104 9 0.1056959246778959 1104 10-11 0.20226111441762384 1104 12-13 0.2818014247937555 1104 14-15 0.3439952183925641 1104 16-17 0.3376041552292861 1104 18-19 0.367443036542916 1104 20-21 0.3822569410225256 1104 22-23 0.3587367641004988 1104 24-25 0.23329447991110186 1104 26-27 0.25352987486621004 1104 28-29 0.35760667399245705 1104 30-31 0.369600143528487 1104 32-33 0.3420477027954263 1104 34-35 0.417279537540864 1104 36-37 0.3368542265066168 1104 38-39 0.3470453817759136 1104 40-41 0.5006511599441694 1104 42-43 0.42274658613433047 1104 44-45 0.42463476383095866 1104 46-47 0.51181799655118 1104 48-49 0.5036224092959358 1104 50-51 0.5185894312577801 1104 52-53 0.5057457484023047 1104 54-55 0.3977056047310512 1104 56-57 0.4752505845739208 1104 58-59 0.4218238901798088 1104 60-61 0.4022009630193466 1104 62-63 0.40980362197161924 1104 64-65 0.4059009649234895 1104 66-67 0.30856879316934993 1104 68-69 0.5712454779766958 1104 70-71 0.5901228567962704 1104 72-73 0.44636406522324634 1104 74-75 0.48503423560133996 1104 76-77 0.47024387641247856 1104 78-79 0.5903230926537297 1104 80-81 0.7396045494738672 1104 82-83 0.699909900309958 1104 84-85 0.744866976889579 1104 86-87 0.6605062940706681 1104 88-89 0.6366625613203531 1104 90-91 0.7781403979337966 1104 92-93 0.7828911137558414 1104 94-95 0.5942059106417261 1104 96-97 1.0090646393736336 1104 98-99 1.0231133699875485 1104 100-101 1.1429986115073305 1105 1 0.2819208133557325 1105 2 0.08389667332429696 1105 3 0.12779494563827143 1105 4 0.1340068226389164 1105 5 0.14644574689958745 1105 6 0.13407482186307362 1105 7 0.24062568241310345 1105 8 0.2787305980469057 1105 9 0.231850143966561 1105 10-11 0.280511118748656 1105 12-13 0.28939805005578023 1105 14-15 0.43378091906647853 1105 16-17 0.41137067810578287 1105 18-19 0.34549034870617135 1105 20-21 0.24202557744127517 1105 22-23 0.2538089190474011 1105 24-25 0.30404833885813787 1105 26-27 0.3746691954143486 1105 28-29 0.31044912847334416 1105 30-31 0.5073389997902851 1105 32-33 0.5087810673121709 1105 34-35 0.38489513271090203 1105 36-37 0.6046720807415014 1105 38-39 0.6501676207447815 1105 40-41 0.5082620776026374 1105 42-43 0.5243256793900528 1105 44-45 0.5846013282853377 1105 46-47 0.39215035973396795 1105 48-49 0.21536772745334787 1105 50-51 0.24733966946439523 1105 52-53 0.26880466772389155 1105 54-55 0.03572207996622012 1105 56-57 0.1845826532467143 1105 58-59 0.10810795372466941 1105 60-61 0.24099139317716123 1105 62-63 0.04733025780136302 1105 64-65 0.1745105313008466 1105 66-67 0.2463080825340782 1105 68-69 0.14881161279298283 1105 70-71 -0.03242621376485744 1105 72-73 0.10393990154498667 1105 74-75 0.052417687604876306 1105 76-77 -0.014503104860150273 1105 78-79 0.2600223459842894 1105 80-81 0.4950226670353288 1105 82-83 0.26483895805123936 1105 84-85 0.38925916024803087 1105 86-87 0.5441761483748451 1105 88-89 0.45536827654652257 1105 90-91 0.6415094189479511 1105 92-93 0.6633495098890982 1105 94-95 0.6943189505045204 1105 96-97 0.6948242152792545 1105 98-99 0.6525444459884504 1105 100-101 0.4967899944915821 1106 1 0.38025584070194185 1106 2 0.23306176697900582 1106 3 0.21009493111816369 1106 4 0.21132641026778032 1106 5 0.2092065038813402 1106 6 0.2752732149708592 1106 7 0.21611573613751034 1106 8 0.2819250223245362 1106 9 0.35221701875481415 1106 10-11 0.45073279243337794 1106 12-13 0.39524723450958277 1106 14-15 0.5499030815949126 1106 16-17 0.48231351727136484 1106 18-19 0.4774660517215139 1106 20-21 0.2739286799048628 1106 22-23 0.4685419910187534 1106 24-25 0.4148585610158264 1106 26-27 0.4232002442503173 1106 28-29 0.42014614217064405 1106 30-31 0.26563240282015244 1106 32-33 0.3682602793541889 1106 34-35 0.252912169640382 1106 36-37 0.18722132811577552 1106 38-39 0.3763764199316597 1106 40-41 0.3255313288489532 1106 42-43 0.44036556129315585 1106 44-45 0.41142481214381377 1106 46-47 0.32503840968313824 1106 48-49 0.11656321653906332 1106 50-51 0.42448017573224206 1106 52-53 0.18971120661358754 1106 54-55 0.23525743643492802 1106 56-57 0.24806699572771862 1106 58-59 0.0747673603361605 1106 60-61 0.16856266271881282 1106 62-63 0.21990176934484396 1106 64-65 0.43313305683204106 1106 66-67 0.4582121530050145 1106 68-69 0.4713976444468173 1106 70-71 0.2828150620688419 1106 72-73 0.39781725501287823 1106 74-75 0.5021272853978154 1106 76-77 0.4867044843175279 1106 78-79 0.290713019233408 1106 80-81 0.32838988062615115 1106 82-83 0.5972260641932472 1106 84-85 0.4754505353024818 1106 86-87 0.3781870868579773 1106 88-89 0.5531878399752017 1106 90-91 0.9218806497693031 1106 92-93 0.8969417851903927 1106 94-95 0.7305175856141481 1106 96-97 1.1317488559065225 1106 98-99 1.0015304582821436 1106 100-101 1.0107583406510123 1107 1 0.1080765072087786 1107 2 0.060915998578146 1107 3 0.12041859450823722 1107 4 0.14982077612728517 1107 5 0.14954215480437938 1107 6 0.17481289370498132 1107 7 0.1589879490360957 1107 8 0.16063036876423809 1107 9 0.3096708667528958 1107 10-11 0.23761123884910518 1107 12-13 0.1721472584846495 1107 14-15 0.21239648845178039 1107 16-17 0.37298002410425113 1107 18-19 0.36766744227534076 1107 20-21 0.2587260905977402 1107 22-23 0.35762374599357827 1107 24-25 0.4458367437058328 1107 26-27 0.44621630754449626 1107 28-29 0.34950361040367994 1107 30-31 0.5324509115710541 1107 32-33 0.5476848777718288 1107 34-35 0.5419843345888324 1107 36-37 0.5117885781757181 1107 38-39 0.5769805915931272 1107 40-41 0.6437331989040871 1107 42-43 0.702067790974283 1107 44-45 0.8000462458527551 1107 46-47 0.7135064029701184 1107 48-49 0.7454867360787389 1107 50-51 0.9311020889424952 1107 52-53 0.8281408397950756 1107 54-55 0.8394626629993027 1107 56-57 0.7412700645361596 1107 58-59 0.8512502181640187 1107 60-61 1.0306125308559544 1107 62-63 0.9014409685776457 1107 64-65 0.9184651114951947 1107 66-67 0.8818971206386692 1107 68-69 0.8262501977913459 1107 70-71 0.8018176993260937 1107 72-73 0.8200661161972498 1107 74-75 0.8812090403726422 1107 76-77 0.8131178144541877 1107 78-79 0.7732639662485141 1107 80-81 0.92154739680932 1107 82-83 1.061615898765293 1107 84-85 1.0835205424950018 1107 86-87 1.2390118291260173 1107 88-89 1.3075704820274687 1107 90-91 1.492700597024161 1107 92-93 1.5521539645238818 1107 94-95 1.665127990792758 1107 96-97 1.7469106876015559 1107 98-99 1.659372702348314 1107 100-101 1.6754142984548999 1108 1 0.07398135269238537 1108 2 0.023716725714109543 1108 3 0.04274375800935104 1108 4 0.11819370493900294 1108 5 0.06376602074391258 1108 6 0.04998537954881499 1108 7 0.04992424106058735 1108 8 0.07819404539768016 1108 9 0.08374136550965972 1108 10-11 0.15628526045423285 1108 12-13 0.18695951950138578 1108 14-15 0.10570934496779216 1108 16-17 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-0.8596586245192412 2316 90-91 -0.9151712979568849 2316 92-93 -1.2359137093288837 2316 94-95 -1.0019411730918861 2316 96-97 -1.0471208859852723 2316 98-99 -1.2044561137364838 2316 100-101 -1.185485001795957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 23.0 6 57.0 7 121.0 8 322.0 9 596.0 10 1068.0 11 1766.0 12 2949.0 13 4059.0 14 5096.0 15 7100.0 16 10060.0 17 14690.0 18 12501.0 19 6625.0 20 5308.0 21 5398.0 22 5368.0 23 5792.0 24 6756.0 25 8081.0 26 10028.0 27 12306.0 28 15579.0 29 19592.0 30 24153.0 31 30992.0 32 39521.0 33 52207.0 34 72697.0 35 105381.0 36 163317.0 37 248316.0 38 190986.0 39 15941.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99329691480636 20.452467303900352 15.334076913466465 20.22015886782682 2 14.972133184999986 23.064598327719537 38.64711297624102 23.31615551103946 3 20.53349168388065 26.768091881939526 30.744838973062905 21.953577461116915 4 12.253994116315909 17.858610545372255 39.37813985064494 30.50925548766689 5 12.267319132604108 39.74828284144933 33.96172103279348 14.022676993153091 6 28.85543542804177 38.8090720813359 17.00607919243414 15.329413298188191 7 26.63882341440101 34.76677202929878 21.573994619626415 17.0204099366738 8 26.383653947115924 32.29853469906477 20.067146048539225 21.25066530528008 9 26.18777358982436 18.15658842314502 22.044867826017693 33.61077016101293 10-11 24.667799347184985 27.067700139578587 27.17075475626248 21.09374575697395 12-13 27.223534736365696 25.356777551482235 27.2479746548993 20.17171305725277 14-15 23.27957485634837 26.297390555018485 28.211478371694742 22.211556216938398 16-17 20.525844620893665 29.571642969126216 28.327114765613402 21.57539764436672 18-19 20.37015413358244 28.70819438862518 30.82780270032478 20.093848777467606 20-21 22.14140267550869 26.19920170141968 30.569024222781337 21.090371400290298 22-23 21.462929370829396 26.34984557676084 30.91166737270492 21.27555767970484 24-25 22.405281000076943 26.837057521623226 30.627763721864916 20.129897756434918 26-27 21.661856310271833 25.929306996386913 32.4214551098024 19.98738158353885 28-29 20.33258910689659 28.093559808694728 31.3182946186228 20.255556465785876 30-31 21.192094349239575 26.41843891371991 30.974707965130545 21.41475877190997 32-33 21.324872878191623 26.87777071231209 31.255060545509266 20.54229586398702 34-35 22.319776996394157 26.3086503511474 30.913598775453494 20.45797387700495 36-37 21.890804415618508 25.75175497752814 31.825176629266373 20.53226397758698 38-39 20.930298869136468 27.414366031187683 31.254984245033405 20.400350854642443 40-41 21.0180116366171 27.288206553348072 31.098101072460686 20.595680737574142 42-43 20.788557881573297 27.63211313579708 29.639896969880752 21.93943201274887 44-45 21.67292223259662 26.284555910638563 29.893699884000853 22.14882197276396 46-47 22.85801958715715 27.666599230869842 29.322396966927943 20.152984215045063 48-49 22.25813269656987 27.599414711064508 29.075626372760983 21.066826219604646 50-51 20.497223591304675 27.959288692646194 30.329272250079093 21.21421546597004 52-53 21.918968227351336 28.91808430856324 27.956364373682664 21.206583090402763 54-55 21.127980682844257 27.7464600678454 28.662531371142396 22.46302787816795 56-57 22.135923121033322 27.675278422846084 28.30030699267119 21.888491463449405 58-59 23.34973389630216 27.13121702556759 28.16431218790691 21.354736890223343 60-61 21.88015109423838 28.08902720185093 29.761862735247448 20.26895896866324 62-63 21.191014124385838 28.002156132213813 29.582188284109794 21.22464145929056 64-65 21.052155167926905 29.130170902687162 28.55556204268562 21.262111886700314 66-67 21.943045316361857 27.89499577055738 27.63991670555159 22.52204220752918 68-69 21.38675870508963 27.846556162627767 28.582374750623668 22.184310381658936 70-71 22.690713703488974 28.116051437812033 27.968869017150013 21.22436584154898 72-73 22.30620259414866 26.960445093959706 29.483446789810053 21.249905522081583 74-75 21.304309651116586 29.861697592131453 27.722622160790696 21.11137059596127 76-77 21.698265210523697 28.146947938211987 27.924453154347834 22.23033369691648 78-79 21.84960550058859 28.354625394148652 28.05998979863852 21.73577930662423 80-81 22.524327301843215 28.44122861530704 27.60614099447394 21.42830308837581 82-83 21.948108728534407 27.98468046125172 28.660545127193632 21.40666568302024 84-85 21.35011131966418 28.665073777848587 28.053596378750278 21.931218523736955 86-87 21.178440739642763 28.782341266698197 27.911986946948215 22.127231046710826 88-89 21.212091038986255 28.6505231342706 27.11576662468075 23.021619202062393 90-91 21.66847928657453 27.397806480987402 27.91579486664232 23.01791936579575 92-93 22.37534804065332 28.33449196021565 28.073348323068625 21.216811676062406 94-95 21.739390181533462 27.7168693511231 28.294191924906425 22.24954854243701 96-97 20.572996106765682 28.00490071392558 29.27284955604818 22.14925362326056 98-99 21.24617094772212 28.86983213724482 27.206231126302605 22.677765788730454 100-101 20.46715948224945 28.08790842736371 27.948147098798316 23.496784991588523 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 900.0 1 610.5 2 371.5 3 413.0 4 371.0 5 394.0 6 642.0 7 1241.5 8 2026.5 9 2409.5 10 2150.0 11 1864.5 12 1891.5 13 1907.5 14 1941.0 15 2089.0 16 2183.5 17 2262.5 18 2364.5 19 2485.0 20 2734.0 21 3084.0 22 3529.5 23 4021.0 24 4528.0 25 5443.5 26 7122.0 27 8650.5 28 9336.5 29 10423.5 30 12397.0 31 14507.5 32 17609.5 33 21710.0 34 26251.5 35 29372.5 36 34154.5 37 39312.5 38 39502.5 39 37932.5 40 35736.5 41 34042.0 42 33158.0 43 33277.0 44 34645.0 45 35474.5 46 35318.5 47 45028.0 48 68876.0 49 65719.5 50 40841.5 51 30623.0 52 27625.5 53 26833.5 54 26034.0 55 24138.0 56 22316.0 57 20497.5 58 18940.5 59 17365.0 60 15058.5 61 12547.0 62 10104.5 63 7807.5 64 5693.5 65 4337.0 66 3273.5 67 2493.5 68 1800.5 69 1057.5 70 695.5 71 498.0 72 355.0 73 275.0 74 191.0 75 148.0 76 104.0 77 46.5 78 26.5 79 17.5 80 16.0 81 10.5 82 8.0 83 5.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017198367784379536 2 0.003349155831694963 3 0.003077602656152128 4 5.431063510856697E-4 5 0.0021724254043426787 6 3.620709007237797E-4 7 0.0 8 0.0 9 0.0 10-11 1.8103545036188986E-4 12-13 5.431063510856697E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0028513083431997654 22-23 0.0 24-25 0.0037112267324187426 26-27 0.001946131091390316 28-29 0.0026702728928378757 30-31 0.016202672807389143 32-33 7.69400664038032E-4 34-35 0.0018556133662093713 36-37 0.0037112267324187426 38-39 0.002262943129523623 40-41 0.004842698297180554 42-43 0.0027607906180188205 44-45 5.883652136761421E-4 46-47 1.3577658777141742E-4 48-49 2.2629431295236236E-4 50-51 1.3577658777141742E-4 52-53 0.0014935424654855913 54-55 0.00348493241946638 56-57 5.883652136761421E-4 58-59 0.0015840601906665364 60-61 0.001357765877714174 62-63 0.0 64-65 4.0732976331425214E-4 66-67 4.5258862590472465E-5 68-69 0.0 70-71 4.0732976331425214E-4 72-73 4.5258862590472465E-5 74-75 2.7155317554283483E-4 76-77 9.956949769903943E-4 78-79 1.3577658777141742E-4 80-81 0.007377194602247011 82-83 0.0020819076791617334 84-85 0.004933216022361499 86-87 0.0030323437935616555 88-89 0.0018556133662093713 90-91 1.3577658777141742E-4 92-93 0.0 94-95 9.051772518094493E-5 96-97 0.0017198367784379536 98-99 0.003982779907961577 100-101 0.0014030247403046465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1104756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.4948688432863 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53266859000635 28.64668232815853 2 14.03630456403917 11.087240156022958 3 5.196925166510413 6.157556335191078 4 2.4126613160319805 3.8115096895981435 5 1.3412162941243613 2.6485580813460077 6 0.8844531548663455 2.0958816809686263 7 0.5755489409690749 1.5911860955526163 8 0.39802045295756855 1.257581246920366 9 0.3107424635159156 1.1045459556540689 >10 1.942526090013287 14.948787462927207 >50 0.22122438921265788 6.067899804997348 >100 0.13522616276924299 9.79345562925704 >500 0.006010051658805667 1.5297338772349793 >1k 0.005316584159712706 5.139252157507083 >5k 9.246233321239489E-4 2.578527311328763 >10k+ 2.311558330309872E-4 1.5416021873352745 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16886 1.5284823074054361 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7697 0.6967149307177332 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7513 0.6800596692844393 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7062 0.639236175227833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5972 0.5405718547806032 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4556 0.4123987559243851 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4509 0.40814442284088065 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4470 0.40461423155882387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4060 0.36750196423463644 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3824 0.3461397810919334 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3553 0.3216094775678973 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3417 0.30929906694328885 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3318 0.3003378121503753 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2762 0.2500099569497699 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2592 0.23462194366900926 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2265 0.20502264753484026 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1819 0.16465174210413883 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1694 0.15333702645652073 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1626 0.14718182114421646 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1573 0.1423843817096264 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1572 0.14229386398444543 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1526 0.13813004862612197 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.1252765316504278 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1338 0.12111271629210432 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1235 0.11178939059846699 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1168 0.10572470301134368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.051772518094493E-5 0.0 18-19 0.0 0.0 0.0 9.051772518094493E-5 0.0 20-21 0.0 0.0 0.0 2.715531755428348E-4 0.0 22-23 0.0 0.0 0.0 4.525886259047246E-4 0.0 24-25 0.0 0.0 0.0 0.0014482836028951189 0.0 26-27 0.0 4.5258862590472465E-5 0.0 0.0022629431295236235 0.0 28-29 0.0 1.357765877714174E-4 0.0 0.004525886259047247 0.0 30-31 0.0 1.8103545036188986E-4 0.0 0.008146595266285044 0.0 32-33 0.0 1.8103545036188986E-4 0.0 0.011359974510208589 0.0 34-35 0.0 1.8103545036188986E-4 0.0 0.01507120124262733 0.0 36-37 0.0 1.8103545036188986E-4 0.0 0.018782427975046073 0.0 38-39 0.0 1.8103545036188986E-4 0.0 0.023579867409636156 0.0 40-41 0.0 1.8103545036188986E-4 0.0 0.03163594495074026 0.0 42-43 0.0 2.715531755428348E-4 0.0 0.041457118132872776 0.0 44-45 0.0 3.620709007237797E-4 0.0 0.06204990061153775 0.0 46-47 0.0 4.5258862590472467E-4 0.0 0.10721824547682927 0.0 48-49 0.0 4.5258862590472467E-4 0.0 0.1350977048325603 0.0 50-51 0.0 4.5258862590472467E-4 0.0 0.16854400428691946 0.0 52-53 0.0 4.5258862590472467E-4 0.0 0.20189978601609768 0.0 54-55 0.0 4.978474884951971E-4 0.0 0.23769954632516138 0.0 56-57 0.0 5.431063510856696E-4 0.0 0.28327522095376717 0.0 58-59 0.0 5.431063510856696E-4 0.0 0.3531549047934567 0.0 60-61 0.0 5.431063510856696E-4 0.0 0.4327199852275072 0.0 62-63 0.0 5.431063510856696E-4 0.0 0.5307054227358802 0.0 64-65 0.0 5.431063510856696E-4 0.0 0.604070038995036 0.0 66-67 0.0 5.431063510856696E-4 0.0 0.7177150429597123 0.0 68-69 0.0 5.431063510856696E-4 0.0 0.8676576547219477 0.0 70-71 0.0 5.431063510856696E-4 0.0 0.9675892233217108 0.0 72-73 0.0 5.431063510856696E-4 0.0 1.1385772061885158 0.0 74-75 0.0 5.431063510856696E-4 0.0 1.28363186079098 0.0 76-77 0.0 5.431063510856696E-4 0.0 1.3894018226649143 0.0 78-79 0.0 5.431063510856696E-4 0.0 1.5376245976487115 0.0 80-81 0.0 5.883652136761421E-4 0.0 1.6780628482669475 0.0 82-83 0.0 6.788829388570869E-4 0.0 1.8566090611863615 0.0 84-85 0.0 7.69400664038032E-4 0.0 2.1208755598521303 0.0 86-87 0.0 8.146595266285044E-4 0.0 2.505847445046689 0.0 88-89 0.0 8.146595266285044E-4 0.0 2.9666279250802896 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTAT 25 0.005020694 57.001522 3 ACTCGCG 20 5.390963E-4 47.496967 66-67 TAGACCG 100 3.031164E-8 37.999294 4 CGGTGTA 110 2.7346832E-6 30.225344 9 CATATAG 355 0.0 24.08515 3 GACCGGT 160 1.8604806E-6 23.748484 6 GGTAAGG 185 2.5408008E-7 23.108725 3 GAGTACT 6360 0.0 22.852314 12-13 CATGGGG 1965 0.0 22.722223 4 GTACATG 6730 0.0 22.309774 1 TAATACC 410 0.0 22.011786 4 CTTTGCG 260 6.2391337E-10 21.925646 2 GGATATG 640 0.0 21.529858 1 GTACTTT 6820 0.0 21.345778 14-15 AGTACTT 6790 0.0 21.335161 12-13 TTTGCGT 245 7.516064E-9 21.3271 3 AGGCTTA 380 0.0 21.248644 6 TACATGG 7070 0.0 21.23304 2 ACATGGG 6820 0.0 21.034286 3 ACTGCGT 115 0.0037245653 20.650856 8 >>END_MODULE