##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1014270 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26824809961845 33.0 31.0 34.0 31.0 34.0 2 32.32352233626155 33.0 31.0 34.0 31.0 34.0 3 32.46199434075739 34.0 31.0 34.0 31.0 34.0 4 36.10426809429442 37.0 35.0 37.0 35.0 37.0 5 36.03594112021454 37.0 35.0 37.0 35.0 37.0 6 36.10046634525324 37.0 35.0 37.0 35.0 37.0 7 36.05096473325643 37.0 35.0 37.0 35.0 37.0 8 36.05069853194909 37.0 35.0 37.0 35.0 37.0 9 37.7372494503436 39.0 37.0 39.0 35.0 39.0 10-11 37.63808749149635 39.0 37.0 39.0 35.0 39.0 12-13 37.59005836710146 39.0 37.0 39.0 35.0 39.0 14-15 38.75727370424049 40.0 38.0 41.0 35.0 41.0 16-17 38.71851923057963 40.0 38.0 41.0 35.0 41.0 18-19 38.80563903102724 40.0 38.0 41.0 35.0 41.0 20-21 38.90414238812151 40.0 38.0 41.0 35.0 41.0 22-23 38.83516272787325 40.0 38.0 41.0 35.0 41.0 24-25 38.720352568842614 40.0 38.0 41.0 34.5 41.0 26-27 38.53040856971023 40.0 38.0 41.0 34.0 41.0 28-29 38.40723574590592 40.0 38.0 41.0 34.0 41.0 30-31 38.35106135447169 40.0 38.0 41.0 34.0 41.0 32-33 38.234500675362575 40.0 38.0 41.0 34.0 41.0 34-35 38.07660386287675 40.0 37.0 41.0 34.0 41.0 36-37 37.87632829522711 40.0 37.0 41.0 33.0 41.0 38-39 37.7253137724669 40.0 37.0 41.0 33.0 41.0 40-41 37.54508759994873 40.0 37.0 41.0 33.0 41.0 42-43 37.28615013753735 40.0 37.0 41.0 32.5 41.0 44-45 36.97066560186144 40.0 36.0 41.0 31.5 41.0 46-47 36.53350587121772 40.0 36.0 41.0 30.5 41.0 48-49 36.092873199443936 39.0 36.0 41.0 29.5 41.0 50-51 35.825454760566714 39.0 35.0 41.0 29.0 41.0 52-53 35.65791357330888 39.0 35.0 40.5 28.0 41.0 54-55 35.42376093150739 39.0 35.0 40.0 28.0 41.0 56-57 35.14484999063366 38.5 35.0 40.0 27.0 41.0 58-59 34.82912636674653 38.0 34.5 40.0 26.0 41.0 60-61 34.42768690782533 38.0 34.0 40.0 26.0 41.0 62-63 34.16057262859002 37.0 34.0 40.0 26.0 41.0 64-65 33.76981277174717 37.0 33.5 39.5 24.5 41.0 66-67 33.30850168101196 36.0 33.0 39.0 23.0 40.0 68-69 32.851836296055296 36.0 32.5 39.0 22.0 40.0 70-71 32.41262336458733 35.0 32.0 38.0 21.5 40.0 72-73 32.02101610024944 35.0 31.5 37.0 20.5 39.5 74-75 31.56312125962515 35.0 31.0 37.0 19.0 39.0 76-77 30.098789769982353 33.5 29.5 35.5 17.5 37.5 78-79 30.805052402220316 34.0 31.0 36.0 17.5 38.0 80-81 30.468537963264218 34.0 31.0 35.5 9.5 37.0 82-83 30.277456692991016 34.0 30.5 35.0 9.5 37.0 84-85 29.966274759186412 34.0 30.0 35.0 7.0 36.5 86-87 29.701082059017814 34.0 30.0 35.0 4.5 36.0 88-89 29.340783519181283 34.0 30.0 35.0 2.0 36.0 90-91 28.910925591804943 34.0 29.0 35.0 2.0 35.0 92-93 28.52818776065545 34.0 29.0 35.0 2.0 35.0 94-95 28.07639435258856 34.0 29.0 35.0 2.0 35.0 96-97 27.48121506107841 33.0 27.0 35.0 2.0 35.0 98-99 26.845546057755826 33.0 26.5 35.0 2.0 35.0 100-101 23.782334092500022 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27656937019164474 1101 2 0.2422999665671881 1101 3 0.18738287047015945 1101 4 0.21990850341001789 1101 5 0.17100811537390115 1101 6 0.23803195338813055 1101 7 0.2191541228489342 1101 8 0.15788796850756626 1101 9 0.21723789994960185 1101 10-11 0.2662550194738458 1101 12-13 0.23408347041750943 1101 14-15 0.2947803947010996 1101 16-17 0.3363945783482194 1101 18-19 0.28110839670013377 1101 20-21 0.2249494850688194 1101 22-23 0.282424294052916 1101 24-25 0.13958366348072815 1101 26-27 0.07532898339695038 1101 28-29 0.176312057185946 1101 30-31 0.2225380778304782 1101 32-33 0.4731325929998391 1101 34-35 0.47854183627526936 1101 36-37 0.4351647584342473 1101 38-39 0.4394864614608167 1101 40-41 0.4756535167049307 1101 42-43 0.49316487919500673 1101 44-45 0.5478650009679171 1101 46-47 0.5125252384155843 1101 48-49 0.5891137244547693 1101 50-51 0.41072422790276875 1101 52-53 0.44929263355346905 1101 54-55 0.5402237143086666 1101 56-57 0.6663582948888163 1101 58-59 0.492152886525858 1101 60-61 0.3568148479784199 1101 62-63 0.39281670629691234 1101 64-65 0.4986745368986334 1101 66-67 0.48363079411404186 1101 68-69 0.4835918220407933 1101 70-71 0.41789519249383744 1101 72-73 0.39110707252803323 1101 74-75 0.4135933189391672 1101 76-77 0.31004093885892203 1101 78-79 0.5193443289465485 1101 80-81 0.6880340137446765 1101 82-83 0.39996814161606764 1101 84-85 0.46068911010851465 1101 86-87 0.5546322212151686 1101 88-89 0.2731009356326304 1101 90-91 0.2206694535371554 1101 92-93 0.45132264655356025 1101 94-95 0.4331136545629235 1101 96-97 0.569970115652989 1101 98-99 0.7938551141319188 1101 100-101 1.2399080485336675 1102 1 0.30305066176718043 1102 2 0.25436331772356624 1102 3 0.1449532379560381 1102 4 0.25969644187389207 1102 5 0.16026973631142738 1102 6 0.22953761174498766 1102 7 0.22478979182371717 1102 8 0.25230996799440675 1102 9 0.24922863339507728 1102 10-11 0.3025279868255737 1102 12-13 0.31231869998996586 1102 14-15 0.36063381780693504 1102 16-17 0.4384120669898408 1102 18-19 0.4043761771903718 1102 20-21 0.3835216667599539 1102 22-23 0.40296251652777215 1102 24-25 0.32377128480930395 1102 26-27 0.4825326735184845 1102 28-29 0.43601525023669296 1102 30-31 0.5541898956245319 1102 32-33 0.4364161807536604 1102 34-35 0.40230726506379 1102 36-37 0.39499937617649294 1102 38-39 0.29692944682260247 1102 40-41 0.45588082449528855 1102 42-43 0.4997996398296465 1102 44-45 0.3953562578846288 1102 46-47 0.40169206527242096 1102 48-49 0.4071680780111109 1102 50-51 0.17882358595955594 1102 52-53 0.32869789763624624 1102 54-55 0.36505308083835075 1102 56-57 0.3736273260999141 1102 58-59 0.4057537333199761 1102 60-61 0.4381277913717483 1102 62-63 0.268569221970111 1102 64-65 0.2501222297889214 1102 66-67 0.3424222644618098 1102 68-69 0.2897610274122968 1102 70-71 0.3618462328774328 1102 72-73 0.32311232621563946 1102 74-75 0.3857297168136036 1102 76-77 0.4237253595466335 1102 78-79 0.38785446135300816 1102 80-81 0.4907134109236253 1102 82-83 0.6694147471382159 1102 84-85 0.452491773417929 1102 86-87 0.49912570814406365 1102 88-89 0.3701514289496082 1102 90-91 0.42863933393491394 1102 92-93 0.7718788543283246 1102 94-95 0.8682061097296128 1102 96-97 0.872180905017899 1102 98-99 1.0052723145137357 1102 100-101 1.200999574734869 1103 1 0.22090380363498952 1103 2 0.21017008691255512 1103 3 0.13803043522663216 1103 4 0.21151349724273416 1103 5 0.16974965619464655 1103 6 0.21806495668846537 1103 7 0.17469134323764024 1103 8 0.13679752612999607 1103 9 0.21566190901717164 1103 10-11 0.20747205784434186 1103 12-13 0.2612591046475998 1103 14-15 0.3367354235373128 1103 16-17 0.265785017392119 1103 18-19 0.3929620820686708 1103 20-21 0.3178925293732462 1103 22-23 0.2325272210122762 1103 24-25 0.15279706815452698 1103 26-27 0.22671953911919474 1103 28-29 0.2845028762678581 1103 30-31 0.17120919494218612 1103 32-33 0.22693880695457125 1103 34-35 0.27093399215752356 1103 36-37 0.2905382124463145 1103 38-39 0.13911975812381883 1103 40-41 0.10888350637056732 1103 42-43 0.1654979458350141 1103 44-45 0.43619841764292033 1103 46-47 0.2556970555973024 1103 48-49 0.08184133054871268 1103 50-51 0.03312646812678821 1103 52-53 0.08838362598604732 1103 54-55 -0.057921933589454966 1103 56-57 -0.10637106138014474 1103 58-59 -0.011902935723036023 1103 60-61 -0.04362102894260289 1103 62-63 0.05431327262021313 1103 64-65 -0.2849267398957096 1103 66-67 -0.3273328022455928 1103 68-69 -0.14201240504858248 1103 70-71 -0.17463406043145113 1103 72-73 -0.21227951613083107 1103 74-75 -0.13311385177790314 1103 76-77 -0.06598124669296723 1103 78-79 -0.006957467899798786 1103 80-81 0.42085437911454804 1103 82-83 0.2882298844570208 1103 84-85 -0.04492395119761383 1103 86-87 0.1005680647995284 1103 88-89 0.050755784450846164 1103 90-91 -0.12269863327152564 1103 92-93 -0.2808518208931865 1103 94-95 -0.34747607107630785 1103 96-97 -0.22581362938818117 1103 98-99 -0.11302724077023996 1103 100-101 0.08319862759157459 1104 1 0.15523588852887116 1104 2 0.11308311229748824 1104 3 0.021020447711030954 1104 4 0.058652490168242366 1104 5 -0.015123419127867521 1104 6 0.12330840238134755 1104 7 0.08438755630464101 1104 8 0.16316040586366398 1104 9 0.20660031933760337 1104 10-11 0.12988674781522036 1104 12-13 0.18741911297053093 1104 14-15 0.1696109957387293 1104 16-17 0.1722769025356925 1104 18-19 0.19655134548939657 1104 20-21 0.255267685428187 1104 22-23 0.17270096216916642 1104 24-25 0.2369054743134953 1104 26-27 0.27276094569430853 1104 28-29 0.1917126140955716 1104 30-31 0.29310370180860446 1104 32-33 0.3640278623020521 1104 34-35 0.30136990559905286 1104 36-37 0.228433551605697 1104 38-39 0.25164576727904375 1104 40-41 0.07195050595441899 1104 42-43 0.027587001182489246 1104 44-45 0.14493229196667556 1104 46-47 0.0028568558469928007 1104 48-49 -0.03312953919743933 1104 50-51 -0.0812305855561064 1104 52-53 -0.18780759332315 1104 54-55 -0.12313208756365412 1104 56-57 -0.25262054119703947 1104 58-59 -0.03007293156156976 1104 60-61 0.06286045253887806 1104 62-63 -0.060007368245365456 1104 64-65 -0.09092445109004643 1104 66-67 -0.12908061747655353 1104 68-69 -0.05295705756626745 1104 70-71 -0.10684379825917034 1104 72-73 0.12634407937478542 1104 74-75 0.2154816538400759 1104 76-77 0.05827489146766496 1104 78-79 0.3362177297697819 1104 80-81 0.3437268302173351 1104 82-83 0.05053221487732529 1104 84-85 0.05214221609327652 1104 86-87 0.048523745199027246 1104 88-89 -0.08748287555748036 1104 90-91 -0.11968677309674192 1104 92-93 -0.11825818793438714 1104 94-95 -0.020842696128326565 1104 96-97 0.1251018928756551 1104 98-99 0.3014026385472839 1104 100-101 0.4734139833968136 1105 1 0.1115910226459107 1105 2 0.08886843844327785 1105 3 0.02398098114827718 1105 4 0.005449485253869568 1105 5 0.0049562500717641456 1105 6 0.017005226437980525 1105 7 0.029165759850783957 1105 8 0.09204265565258396 1105 9 0.10547672383199114 1105 10-11 0.07499239610167763 1105 12-13 0.037257284908783106 1105 14-15 0.136387532293476 1105 16-17 0.1841055055102956 1105 18-19 0.24442322529282023 1105 20-21 0.2409737753591159 1105 22-23 0.04140915207629092 1105 24-25 0.15293890073855465 1105 26-27 0.1275892672288066 1105 28-29 0.12592869515032135 1105 30-31 0.049476696547841925 1105 32-33 0.0703288733215075 1105 34-35 0.1331877922003386 1105 36-37 0.006195994505823421 1105 38-39 0.15127989513945295 1105 40-41 0.3352269017891061 1105 42-43 0.27699976557382655 1105 44-45 0.30228437354101345 1105 46-47 0.42610489117854655 1105 48-49 0.2157419942181633 1105 50-51 0.09818748289878698 1105 52-53 0.15808229864713752 1105 54-55 0.25902839824527035 1105 56-57 0.04037214136016587 1105 58-59 0.22251956481218116 1105 60-61 0.36295679165255734 1105 62-63 0.303994818327773 1105 64-65 0.2691971093871075 1105 66-67 0.21006283038125417 1105 68-69 0.2581695688328196 1105 70-71 -0.04081903152971478 1105 72-73 0.11240738174970843 1105 74-75 -0.038171655246113545 1105 76-77 -0.039999122950728605 1105 78-79 0.32737672178121 1105 80-81 0.6626147088084053 1105 82-83 0.3609525348530802 1105 84-85 0.23405806550116637 1105 86-87 0.2308265747545697 1105 88-89 0.17456492596229722 1105 90-91 0.16343656261800632 1105 92-93 0.13824740839390515 1105 94-95 0.27964874159763653 1105 96-97 0.08774342477580532 1105 98-99 0.18840714158899985 1105 100-101 0.7719028665125727 1106 1 0.11220359399138857 1106 2 0.11631963268474266 1106 3 0.06890045619099538 1106 4 -0.032599011492415286 1106 5 -0.061137835070205426 1106 6 0.12026621455009945 1106 7 -5.357706687192376E-4 1106 8 0.04396034933055404 1106 9 0.08440754144205442 1106 10-11 -0.0011145948460864474 1106 12-13 -0.018082265023956268 1106 14-15 0.07889125861768065 1106 16-17 -0.002716030825908433 1106 18-19 0.011567952089635014 1106 20-21 0.11728980400637568 1106 22-23 0.11838501276629643 1106 24-25 0.020922504912036288 1106 26-27 0.22038655519186534 1106 28-29 0.16940853664521427 1106 30-31 -0.13652141302426912 1106 32-33 0.06186193763242187 1106 34-35 0.0295905260499012 1106 36-37 0.10071466248124494 1106 38-39 0.11549991186246444 1106 40-41 0.05236708428810033 1106 42-43 -0.12630848323556876 1106 44-45 -0.17712268648006813 1106 46-47 -0.11558177039849937 1106 48-49 -0.20367579034576977 1106 50-51 -0.5523033012652476 1106 52-53 -0.38122678631304296 1106 54-55 -0.5466710600744307 1106 56-57 -0.38780886711040097 1106 58-59 -0.24807519288585667 1106 60-61 -0.30663640280703675 1106 62-63 -0.2945773632987283 1106 64-65 -0.22440088385098278 1106 66-67 -0.4139156114699105 1106 68-69 -0.34907040644620224 1106 70-71 -0.40812462646918846 1106 72-73 -0.430439893489325 1106 74-75 -0.4744660530357656 1106 76-77 -0.7239913794672539 1106 78-79 -0.5885437711842165 1106 80-81 -0.23114597029146466 1106 82-83 -0.1417404160321496 1106 84-85 -0.19544806021228922 1106 86-87 -0.2792799435862179 1106 88-89 -0.6106261163178495 1106 90-91 -0.40710639008494454 1106 92-93 -0.17747131774853386 1106 94-95 -0.13797222761020222 1106 96-97 -0.041100142316206956 1106 98-99 0.04548079556798612 1106 100-101 0.043025671616728545 1107 1 0.12356421052074751 1107 2 0.018516461456115962 1107 3 0.01764702364604176 1107 4 0.03280979353903035 1107 5 0.08730312515656635 1107 6 -0.026301240494639444 1107 7 0.06125889670660456 1107 8 0.027590431711630004 1107 9 -0.06682374565158256 1107 10-11 -0.04259966881188859 1107 12-13 0.1356005235312736 1107 14-15 0.2233681830573957 1107 16-17 0.11465043210260717 1107 18-19 0.18901059537070353 1107 20-21 0.08340504893507017 1107 22-23 0.09943515006809633 1107 24-25 0.11468413127398236 1107 26-27 0.06471510510771594 1107 28-29 0.07276578706598258 1107 30-31 0.14841968007520734 1107 32-33 0.045026965535683416 1107 34-35 -0.00981066094628602 1107 36-37 0.11174974087614942 1107 38-39 0.0858072907268479 1107 40-41 0.23981725702262935 1107 42-43 0.28475981333631495 1107 44-45 0.17238125541026506 1107 46-47 0.21176121835160444 1107 48-49 0.38056329194672855 1107 50-51 0.4569136355759227 1107 52-53 0.33266505530268375 1107 54-55 0.2958991410062737 1107 56-57 0.38401104607942926 1107 58-59 0.5518229380380504 1107 60-61 0.5020623304731444 1107 62-63 0.6438737838356587 1107 64-65 0.44182730287946015 1107 66-67 0.2712978706808542 1107 68-69 0.4454419507156331 1107 70-71 0.3023612447328716 1107 72-73 0.36137071861094583 1107 74-75 0.2968157256868835 1107 76-77 0.4369595462126128 1107 78-79 0.42380674701934495 1107 80-81 0.5942437896518484 1107 82-83 0.5513036977068886 1107 84-85 0.4688866904299296 1107 86-87 0.5471838707463199 1107 88-89 0.3487148350507425 1107 90-91 0.5898796139898188 1107 92-93 0.42777493967019353 1107 94-95 0.42464182146411744 1107 96-97 0.5313199126045127 1107 98-99 0.6476665516136961 1107 100-101 0.610956843554014 1108 1 0.07746946020064627 1108 2 -0.02484674810428089 1108 3 -0.03262613042993223 1108 4 0.08765827838751505 1108 5 0.022241238686227405 1108 6 0.12321647183997442 1108 7 0.11718292572922451 1108 8 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-1.309942783589328 2316 86-87 -1.2132502026204364 2316 88-89 -1.1667661866728203 2316 90-91 -1.1952739628383817 2316 92-93 -0.9608659451869705 2316 94-95 -0.9473662154579792 2316 96-97 -0.9978318340335086 2316 98-99 -1.291495539577518 2316 100-101 -1.7795838582841341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 4.0 5 20.0 6 42.0 7 114.0 8 265.0 9 476.0 10 897.0 11 1682.0 12 2546.0 13 3671.0 14 4574.0 15 6270.0 16 8792.0 17 13104.0 18 11536.0 19 5956.0 20 4841.0 21 4852.0 22 4968.0 23 5474.0 24 6104.0 25 7133.0 26 8888.0 27 10996.0 28 13940.0 29 17358.0 30 21965.0 31 27079.0 32 35105.0 33 47397.0 34 65373.0 35 96967.0 36 150794.0 37 232326.0 38 178428.0 39 14330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16197398396434 20.5068097318514 15.257546918608664 20.073669365575597 2 15.061847690510113 23.130592544752684 38.551349112834444 23.25621065190276 3 20.654555422549237 26.911691912668097 30.55906696616096 21.874685698621708 4 12.328176915417 17.81182525363069 39.460991649166395 30.399006181785914 5 12.29307684343895 39.823014084923656 33.8515388900666 14.032370181570785 6 28.90581403373855 38.92218048448638 16.94637522553166 15.225630256243406 7 26.64724383053822 34.84979344750412 21.597306437141985 16.90565628481568 8 26.35099761503112 32.45825318529897 20.100979128810994 21.089770070858915 9 26.264899878730514 18.261212497658413 21.911916945192107 33.56197067841896 10-11 24.6277125420253 27.194731185976124 27.18871700829168 20.9888392637069 12-13 27.209139188351816 25.466604290082667 27.214068844621668 20.11018767694385 14-15 23.236268449229495 26.417521961607854 28.14536563242529 22.200843956737355 16-17 20.533221725805344 29.654130045446546 28.224035351585897 21.588612877162213 18-19 20.357690599307748 28.96403522480388 30.708267417056412 19.97000675883196 20-21 22.16539898930892 26.377696371677185 30.44604594597126 21.01085869304264 22-23 21.511530460331073 26.602088201366502 30.750096128249876 21.13628521005255 24-25 22.370497452469515 27.076736498827913 30.45766810373515 20.095097944967424 26-27 21.733840394158612 26.14554565075709 32.26207367106056 19.858540284023736 28-29 20.34336659787634 28.307235407856297 31.16610940915585 20.183288585111512 30-31 21.17361228909674 26.59550756825632 30.84246902281325 21.388411119833684 32-33 21.298272735966904 27.15331230657137 31.058216655837782 20.490198301623938 34-35 22.325815277675073 26.508861445931625 30.78902610367522 20.376297172718086 36-37 21.90046828362072 25.90124136081307 31.711843365094577 20.486446990471627 38-39 20.87591852631527 27.641202237247857 31.132847067987584 20.35003216844928 40-41 20.996902889157745 27.53179690604444 30.94364757028154 20.52765263451627 42-43 20.821153839517123 27.92853864315714 29.425328384132648 21.824979133193086 44-45 21.638939857489493 26.474476973421506 29.791341505618483 22.095241663470517 46-47 22.898745915066915 27.851639469931094 29.198206942993586 20.051407672008402 48-49 22.257552139563362 27.84128946639336 28.944815326413924 20.956343067629348 50-51 20.566369988690937 28.195795632355942 30.19859381146898 21.039240567484132 52-53 21.95421468527711 29.06707380499658 27.829045283950887 21.149666225775427 54-55 21.080585972239476 27.952538141634275 28.602479082983812 22.364396803142434 56-57 22.15411023395359 27.86689027774902 28.233413293028125 21.745586195269265 58-59 23.310155458549726 27.31544997202387 28.07418234521312 21.30021222421328 60-61 21.86139877024795 28.312839930610156 29.662727706985176 20.163033592156722 62-63 21.19435947617369 28.08716635404368 29.573953616772698 21.14452055300993 64-65 21.009184714820275 29.3974965119776 28.46822818322101 21.12509058998112 66-67 21.869930121640106 28.009169223943502 27.64659050295165 22.474310151464735 68-69 21.27945078927351 28.030511049730567 28.467235326798818 22.222802834197104 70-71 22.626792267540115 28.342624563949766 27.93290002741028 21.097683141099843 72-73 22.137186294090647 27.203268955717935 29.438498284025037 21.221046466166378 74-75 21.278331710563613 30.117652858745085 27.59293540554864 21.011080025142657 76-77 21.720804142282248 28.23672494277642 27.91608555707889 22.126385357862443 78-79 21.826112293541073 28.546587329642577 27.958659158934857 21.668641217881497 80-81 22.60295109379375 28.556062105873398 27.49337453217493 21.347612268157924 82-83 21.961418436919196 28.123770600908095 28.678189535749322 21.23662142642339 84-85 21.351041446884533 28.87390043203827 27.950026718934556 21.825031402142642 86-87 21.120510317765486 28.901328048744418 27.93516519270017 22.042996440789928 88-89 21.15133484346924 28.8703805493181 26.983630882690047 22.994653724522617 90-91 21.670129774096942 27.63796354494029 27.775403310287032 22.916503370675738 92-93 22.357442827022627 28.611260233360298 27.943093963777837 21.088202975839238 94-95 21.69813692582162 28.014635235680636 28.08429162215576 22.20293621634199 96-97 20.558088862378177 28.2249214948756 29.161461748162466 22.05552789458376 98-99 21.241515436987346 29.152931398342748 27.05757893496643 22.547974229703478 100-101 20.442332122763336 28.45156643682762 27.72956257292875 23.3765388674803 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 886.0 1 597.5 2 326.5 3 373.5 4 356.5 5 360.5 6 595.0 7 1159.0 8 1829.0 9 2150.5 10 1954.5 11 1681.0 12 1691.0 13 1778.5 14 1888.0 15 1977.5 16 2044.5 17 2108.0 18 2145.5 19 2286.5 20 2474.0 21 2815.0 22 3269.0 23 3657.0 24 4168.5 25 5148.0 26 6617.5 27 7816.0 28 8564.5 29 9780.5 30 11562.5 31 13504.5 32 16348.5 33 20139.0 34 24483.0 35 27396.5 36 31821.5 37 36535.0 38 36671.0 39 35085.0 40 32944.0 41 31458.5 42 30464.0 43 30457.0 44 31969.5 45 32812.0 46 32547.0 47 41336.0 48 62888.0 49 59826.0 50 37286.5 51 28324.0 52 25430.0 53 24413.5 54 23398.0 55 21776.5 56 20329.5 57 18460.0 58 17039.0 59 15690.5 60 13651.0 61 11328.5 62 9189.0 63 7021.0 64 5059.0 65 3889.0 66 2887.0 67 2175.0 68 1530.5 69 918.5 70 620.5 71 444.0 72 327.0 73 251.0 74 188.5 75 138.5 76 96.5 77 42.5 78 20.0 79 13.0 80 8.5 81 6.0 82 4.0 83 3.0 84 3.0 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027606061502361307 2 0.008380411527502539 3 0.011436796908121112 4 0.0 5 0.006408549991619589 6 0.0 7 0.0 8 9.859307679414751E-5 9 0.0 10-11 0.0 12-13 4.9296538397073754E-5 14-15 0.0 16-17 1.478896151912213E-4 18-19 0.004091612686957122 20-21 0.0013310065367209915 22-23 0.0 24-25 0.005176136531692745 26-27 0.005816991530854704 28-29 0.0075916669131493595 30-31 0.02938073688465596 32-33 6.901515375590326E-4 34-35 0.004436688455736638 36-37 0.013901623827974802 38-39 0.007640963451546432 40-41 0.009760714602620603 42-43 0.009810011141017677 44-45 0.002859199227030278 46-47 0.002415530381456614 48-49 0.0020704546126770976 50-51 0.003845129994971753 52-53 0.0035986473029863846 54-55 0.0023662338430595407 56-57 8.873376911473276E-4 58-59 0.001232413459926844 60-61 4.9296538397073754E-5 62-63 2.4648269198536876E-4 64-65 0.008380411527502539 66-67 7.394480759561064E-4 68-69 0.001429599613515139 70-71 0.005126839993295671 72-73 0.012176244984077218 74-75 0.005669101915663482 76-77 0.00458457807092786 78-79 0.011387500369724038 80-81 1.9718615358829501E-4 82-83 0.005422619223678113 84-85 7.887446143531801E-4 86-87 0.0 88-89 0.001232413459926844 90-91 7.394480759561064E-4 92-93 1.478896151912213E-4 94-95 5.422619223678113E-4 96-97 4.929653839707375E-4 98-99 1.478896151912213E-4 100-101 9.366342295444013E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1014270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.13477182280979 #Duplication Level Percentage of deduplicated Percentage of total 1 73.14903038928244 29.358196437336296 2 13.741732238267518 11.030425756660321 3 4.978727289337524 5.994602511766735 4 2.3676284054667383 3.800969032584421 5 1.3560982268643047 2.721334645225791 6 0.8261241738623711 1.9893783129164109 7 0.5742085164312412 1.6132009449977396 8 0.40234907834239736 1.291855076191195 9 0.3149352609575595 1.1375869353739836 >10 1.9292053326763747 14.998166327724881 >50 0.21805466440855528 6.047695477845686 >100 0.13053413228087105 9.662404002371524 >500 0.00494447470531889 1.3302784981211955 >1k 0.005191698440584834 4.895647131893771 >5k 9.88894941063778E-4 2.521744464067292 >10k+ 2.472237352659445E-4 1.6065144449228221 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16191 1.5963205063740424 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7170 0.7069123606140376 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6539 0.6447001291569306 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6435 0.6344464491703392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5271 0.5196841077819515 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4313 0.42523194021315824 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4253 0.4193163556055094 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3839 0.3784988218127323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3645 0.3593717649146677 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3211 0.31658236958600766 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3188 0.31431472881974226 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3063 0.30199059422047386 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3059 0.30159622191329727 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2574 0.2537785796681357 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2325 0.22922890354639297 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2138 0.2107919981858874 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1668 0.16445325209263806 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1544 0.15222771057016377 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1518 0.14966429057351593 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1505 0.14838258057519202 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1421 0.14010076212448364 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1366 0.13467814290080551 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1265 0.1247202421445966 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1222 0.12048073984244827 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1123 0.11072002523982766 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1100 0.10845238447356226 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.929653839707376E-5 0.0 12-13 0.0 0.0 0.0 9.859307679414752E-5 0.0 14-15 0.0 0.0 0.0 9.859307679414752E-5 0.0 16-17 0.0 0.0 0.0 2.4648269198536876E-4 0.0 18-19 0.0 0.0 0.0 2.9577923038244254E-4 0.0 20-21 0.0 0.0 0.0 2.9577923038244254E-4 0.0 22-23 0.0 0.0 0.0 4.929653839707375E-4 0.0 24-25 0.0 0.0 0.0 9.85930767941475E-4 0.0 26-27 0.0 0.0 0.0 0.0015774892287063603 0.0 28-29 0.0 4.929653839707376E-5 0.0 0.0033028680726039416 0.0 30-31 0.0 9.859307679414752E-5 0.0 0.0069508119139874 0.0 32-33 0.0 9.859307679414752E-5 0.0 0.011387500369724038 0.0 34-35 0.0 9.859307679414752E-5 0.0 0.01454247882713676 0.0 36-37 0.0 9.859307679414752E-5 0.0 0.018190422668520216 0.0 38-39 0.0 9.859307679414752E-5 0.0 0.02321866958502174 0.0 40-41 0.0 9.859307679414752E-5 0.0 0.030563853806185728 0.0 42-43 0.0 1.9718615358829504E-4 0.0 0.04081753379277707 0.0 44-45 0.0 2.4648269198536876E-4 0.0 0.06097981799718024 0.0 46-47 0.0 2.9577923038244254E-4 0.0 0.10648052293767932 0.0 48-49 0.0 2.9577923038244254E-4 0.0 0.13531899789996749 0.0 50-51 0.0 2.9577923038244254E-4 0.0 0.17278436708174352 0.0 52-53 0.0 2.9577923038244254E-4 0.0 0.21069340510909323 0.0 54-55 0.0 3.450757687795163E-4 0.0 0.2528419454385913 0.0 56-57 0.0 3.943723071765901E-4 0.0 0.30189200114367964 0.0 58-59 0.0 3.943723071765901E-4 0.0 0.3728790164354659 0.0 60-61 0.0 3.943723071765901E-4 0.0 0.4630916817021109 0.0 62-63 0.0 3.943723071765901E-4 0.0 0.573515927711556 0.0 64-65 0.0 3.943723071765901E-4 0.0 0.6519960168396975 0.0 66-67 0.0 3.943723071765901E-4 0.0 0.7778007828290298 0.0 68-69 0.0 3.943723071765901E-4 0.0 0.9395427253098287 0.0 70-71 0.0 3.943723071765901E-4 0.0 1.042720380174904 0.0 72-73 0.0 3.943723071765901E-4 0.0 1.217772388022913 0.0 74-75 0.0 3.943723071765901E-4 0.0 1.3709860293610183 0.0 76-77 0.0 3.943723071765901E-4 0.0 1.4880653080540684 0.0 78-79 0.0 3.943723071765901E-4 0.0 1.6461593066934839 0.0 80-81 0.0 3.943723071765901E-4 0.0 1.7897108265057629 0.0 82-83 0.0 4.436688455736638E-4 0.0 1.9830025535606892 0.0 84-85 0.0 4.929653839707375E-4 0.0 2.2624646297337003 0.0 86-87 0.0 4.929653839707375E-4 0.0 2.6532382896073035 0.0 88-89 0.0 4.929653839707375E-4 0.0 3.1455628185788793 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 70 0.00839768 27.138504 5 CATATAG 320 0.0 26.718418 3 GTACATG 6715 0.0 24.213297 1 AGTACTT 6240 0.0 23.137316 12-13 GAGTACT 5785 0.0 22.658422 12-13 ACATGGG 6625 0.0 22.584623 3 TACATGG 7235 0.0 21.931097 2 CCTAGAC 195 4.2209285E-7 21.922804 3 CGGCAAC 55 0.0028758552 21.593834 40-41 CATTCCG 265 7.9489837E-10 21.504925 9 GAATAAC 445 0.0 21.367012 1 AGAGTAC 10560 0.0 20.866835 10-11 CATGGGA 4495 0.0 20.602924 4 TAAGGTG 395 0.0 20.43976 5 GTACTTT 6430 0.0 20.35011 14-15 CTGGTCG 470 0.0 20.208529 9 GGCCCGC 285 1.9681465E-9 19.995806 9 TAGACTA 120 0.004767541 19.788492 5 CTCCCGC 290 2.4447218E-9 19.65105 7 GTAAGGT 340 4.1836756E-11 19.555689 4 >>END_MODULE