##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937081_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1055764 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27254007524409 33.0 31.0 34.0 31.0 34.0 2 32.37857324174721 34.0 31.0 34.0 31.0 34.0 3 32.48489908729602 34.0 31.0 34.0 31.0 34.0 4 36.103112059134425 37.0 35.0 37.0 35.0 37.0 5 36.02611284340061 37.0 35.0 37.0 35.0 37.0 6 36.09899655604851 37.0 35.0 37.0 35.0 37.0 7 36.0499372965928 37.0 35.0 37.0 35.0 37.0 8 36.020711068003834 37.0 35.0 37.0 35.0 37.0 9 37.703108838717746 39.0 37.0 39.0 35.0 39.0 10-11 37.618524120920966 39.0 37.0 39.0 35.0 39.0 12-13 37.554239868000806 39.0 37.0 39.0 35.0 39.0 14-15 38.706503536775266 40.0 38.0 41.0 35.0 41.0 16-17 38.65680303552688 40.0 38.0 41.0 34.5 41.0 18-19 38.85681459113968 40.0 38.0 41.0 35.0 41.0 20-21 38.89599664318919 40.0 38.0 41.0 35.0 41.0 22-23 38.809581023789406 40.0 38.0 41.0 35.0 41.0 24-25 38.67779115408368 40.0 38.0 41.0 34.0 41.0 26-27 38.47931403230267 40.0 38.0 41.0 34.0 41.0 28-29 38.42350942066598 40.0 38.0 41.0 34.0 41.0 30-31 38.4105751853634 40.0 38.0 41.0 34.0 41.0 32-33 38.20885680890805 40.0 37.5 41.0 34.0 41.0 34-35 38.02151948730966 40.0 37.0 41.0 34.0 41.0 36-37 37.828593321992415 40.0 37.0 41.0 33.0 41.0 38-39 37.6686210175759 40.0 37.0 41.0 33.0 41.0 40-41 37.533691715193925 40.0 37.0 41.0 33.0 41.0 42-43 37.23803331047469 40.0 36.5 41.0 32.5 41.0 44-45 36.90574456033735 40.0 36.0 41.0 31.0 41.0 46-47 36.46395832780811 39.5 36.0 41.0 30.5 41.0 48-49 36.018800603165104 39.0 35.5 41.0 29.5 41.0 50-51 35.7150949454613 39.0 35.0 40.5 28.0 41.0 52-53 35.52269493939934 39.0 35.0 40.0 28.0 41.0 54-55 35.2630441083424 39.0 35.0 40.0 27.0 41.0 56-57 34.95833491196896 38.0 34.5 40.0 26.5 41.0 58-59 34.6180983628917 38.0 34.0 40.0 26.0 41.0 60-61 34.22968911612823 38.0 34.0 40.0 25.0 41.0 62-63 33.9123383634979 37.0 33.5 40.0 25.0 41.0 64-65 33.55137464433339 36.5 33.0 39.0 23.5 40.5 66-67 33.11940215805805 36.0 33.0 39.0 22.0 40.0 68-69 32.78871556522101 36.0 32.5 39.0 22.0 40.0 70-71 32.2863210906983 35.0 32.0 38.0 20.5 40.0 72-73 31.784095688051494 35.0 31.0 37.0 19.5 39.0 74-75 31.2901192880227 35.0 31.0 37.0 18.0 39.0 76-77 29.878776885743406 33.5 29.5 35.0 16.0 37.5 78-79 30.626372939406913 34.0 31.0 36.0 16.0 37.5 80-81 30.35702770694966 34.0 31.0 35.5 9.5 37.0 82-83 30.03989812117102 34.0 30.0 35.0 7.5 37.0 84-85 29.687454772089218 34.0 30.0 35.0 6.5 36.0 86-87 29.439816568854404 34.0 30.0 35.0 2.0 36.0 88-89 29.060038512394815 34.0 29.0 35.0 2.0 35.5 90-91 28.605820997874524 34.0 29.0 35.0 2.0 35.0 92-93 28.16200164051815 33.5 29.0 35.0 2.0 35.0 94-95 27.607006395368664 33.0 27.0 35.0 2.0 35.0 96-97 26.960263846844562 33.0 26.0 34.5 2.0 35.0 98-99 26.23279539745625 33.0 25.0 34.0 2.0 35.0 100-101 22.79370531671851 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12137602784691381 1101 2 0.17274696965783676 1101 3 0.10663729096236807 1101 4 0.11904625747217068 1101 5 0.18054114319525638 1101 6 0.12683116404519268 1101 7 0.1370736685371412 1101 8 0.19251514729838703 1101 9 0.3089988117824234 1101 10-11 0.23942681030768398 1101 12-13 0.29847467944008343 1101 14-15 0.2896108114450229 1101 16-17 0.2709731299287128 1101 18-19 0.19153932679896002 1101 20-21 0.28082422149605435 1101 22-23 0.1484400108341859 1101 24-25 0.1668450448973786 1101 26-27 0.13800442017632264 1101 28-29 0.21199593399614258 1101 30-31 0.3725185885721274 1101 32-33 0.37536264259534136 1101 34-35 0.37812489748672107 1101 36-37 0.4501297123117638 1101 38-39 0.2909856428365103 1101 40-41 0.4269444926212529 1101 42-43 0.32131069853764416 1101 44-45 0.16611126242916185 1101 46-47 0.060511802288139904 1101 48-49 0.18325076579913713 1101 50-51 -0.03296518670533288 1101 52-53 0.03422702314081505 1101 54-55 0.038690911809133866 1101 56-57 0.0640344646592439 1101 58-59 -0.014192061616071783 1101 60-61 0.047190829654930155 1101 62-63 -0.10176946157464073 1101 64-65 -0.17125153653177705 1101 66-67 -0.2865830563286238 1101 68-69 -0.12149494889581547 1101 70-71 -0.19535145498758055 1101 72-73 -0.17141626882608207 1101 74-75 -0.201953137958494 1101 76-77 -0.35896002588761533 1101 78-79 -0.2642331888859317 1101 80-81 -0.03516827456030924 1101 82-83 0.029284948815838874 1101 84-85 -0.09817436638347843 1101 86-87 -0.6038343797325112 1101 88-89 -0.38755263717440513 1101 90-91 -0.16570373065912847 1101 92-93 -0.09430337566812952 1101 94-95 -0.15126708651562737 1101 96-97 -0.21353891231878563 1101 98-99 0.009224845117838498 1101 100-101 0.7376491847791371 1102 1 0.1172566130832351 1102 2 0.11844266810820869 1102 3 0.1600118664255632 1102 4 0.15968240010806767 1102 5 0.13618609621360633 1102 6 0.14157384245226723 1102 7 0.05873620167663063 1102 8 0.12300415104353846 1102 9 0.19185417163637197 1102 10-11 0.23018585799043478 1102 12-13 0.18370600595937248 1102 14-15 0.245239676307051 1102 16-17 0.2731192053076512 1102 18-19 0.20054573073192472 1102 20-21 0.1977047618548866 1102 22-23 0.16006582064000696 1102 24-25 0.23274086307750252 1102 26-27 0.23232970406878195 1102 28-29 0.31002022372520344 1102 30-31 0.2411858880396167 1102 32-33 0.23670559721065842 1102 34-35 0.2172251292408518 1102 36-37 0.1294453390767032 1102 38-39 0.21381964777980755 1102 40-41 0.2144144437555937 1102 42-43 0.3033203294160671 1102 44-45 0.3533604671238564 1102 46-47 0.596117432465455 1102 48-49 0.41447271207655234 1102 50-51 0.24745391823432072 1102 52-53 0.3865258936675673 1102 54-55 0.49705603336362714 1102 56-57 0.24297413661327028 1102 58-59 0.44688306753371876 1102 60-61 0.32064672651460313 1102 62-63 0.32816450077738324 1102 64-65 0.29482192876395885 1102 66-67 0.29711430682255724 1102 68-69 0.2567431100935238 1102 70-71 0.21519712574067995 1102 72-73 0.3901315134215757 1102 74-75 0.39847844800348753 1102 76-77 0.2176691327949527 1102 78-79 0.24828763045846713 1102 80-81 0.23391164377959583 1102 82-83 0.3141236396463505 1102 84-85 0.4475553712186908 1102 86-87 0.14021744783970647 1102 88-89 0.18862562975858665 1102 90-91 0.3328131953514415 1102 92-93 0.15193644116397564 1102 94-95 0.0907864226923003 1102 96-97 0.09819113180509831 1102 98-99 0.1401511080169442 1102 100-101 0.6475682105481297 1103 1 0.36237972005287133 1103 2 0.4068622179173573 1103 3 0.33861252930253016 1103 4 0.22295154596019984 1103 5 0.23042266195937344 1103 6 0.23938833812994886 1103 7 0.1380013123219328 1103 8 0.20731152489532434 1103 9 0.3751646120359169 1103 10-11 0.3688009722136698 1103 12-13 0.28890892694645487 1103 14-15 0.4544512750832226 1103 16-17 0.5342264960816507 1103 18-19 0.40250940339846153 1103 20-21 0.3857398150962368 1103 22-23 0.30973062969199106 1103 24-25 0.2557840408377885 1103 26-27 0.4232227379264657 1103 28-29 0.4218868210440263 1103 30-31 0.35972445201561953 1103 32-33 0.3387023439124164 1103 34-35 0.3882187640162371 1103 36-37 0.32630304393064335 1103 38-39 0.28009730910518016 1103 40-41 0.3419135405464502 1103 42-43 0.21290036770864162 1103 44-45 0.18428756985582595 1103 46-47 -0.03188519251337851 1103 48-49 0.030547740394553102 1103 50-51 -0.12074692406274323 1103 52-53 -0.18684610458011974 1103 54-55 -0.32716812307393894 1103 56-57 -0.10063149173231523 1103 58-59 -0.13369278719883226 1103 60-61 0.058207747673264976 1103 62-63 0.18500105221143315 1103 64-65 0.04363117622929735 1103 66-67 0.010070078372599767 1103 68-69 -0.20682440020829063 1103 70-71 -0.3420615341587592 1103 72-73 -0.13256085267222062 1103 74-75 0.038384671332636344 1103 76-77 -0.02735492660217531 1103 78-79 0.13206817594442555 1103 80-81 0.14171318909625796 1103 82-83 0.0026220784471711056 1103 84-85 -0.03357453905380581 1103 86-87 -0.18800657246199037 1103 88-89 -0.16835316620690222 1103 90-91 -0.11328267222675592 1103 92-93 -0.16493288759891556 1103 94-95 -0.09950272640614699 1103 96-97 -0.07241765733614614 1103 98-99 -0.1712064633191268 1103 100-101 0.7125844334060432 1104 1 0.25659799691030827 1104 2 0.28742955508258916 1104 3 0.17092301108751684 1104 4 0.15821447947148215 1104 5 0.16787284639281808 1104 6 0.132483500395999 1104 7 0.12419281641923163 1104 8 0.18171243214948873 1104 9 0.24369364366342694 1104 10-11 0.24349070443550858 1104 12-13 0.1577315860571673 1104 14-15 0.08048908183337034 1104 16-17 0.15274373205703995 1104 18-19 0.27322147321524426 1104 20-21 0.34185661901373976 1104 22-23 0.3197914784060103 1104 24-25 0.32619776491052477 1104 26-27 0.2935008962544501 1104 28-29 0.2803807016282107 1104 30-31 0.370511575881757 1104 32-33 0.2375348271162494 1104 34-35 0.290981305743351 1104 36-37 0.23235657259997566 1104 38-39 0.3118516258925794 1104 40-41 0.030028915504949794 1104 42-43 0.2640854937283379 1104 44-45 0.04134701488724346 1104 46-47 0.06048318907259187 1104 48-49 -0.17920591583632728 1104 50-51 -0.15560265481181546 1104 52-53 -0.12816442036320552 1104 54-55 -0.18450729356997897 1104 56-57 -0.2628061213067099 1104 58-59 -0.18906663354193398 1104 60-61 -0.024073244981416053 1104 62-63 0.011691748306567717 1104 64-65 -0.07767446765322461 1104 66-67 -0.062133635909738416 1104 68-69 -0.09663742227122185 1104 70-71 -0.5016992559802098 1104 72-73 -0.1041809349384657 1104 74-75 -0.1467459823655517 1104 76-77 -0.14280108029278438 1104 78-79 -0.11128328899130935 1104 80-81 0.06540922188143483 1104 82-83 0.06033314900370712 1104 84-85 -8.555064320923123E-4 1104 86-87 -0.07966561408210637 1104 88-89 -0.12011063411851453 1104 90-91 0.07220943395433466 1104 92-93 0.17341781061943706 1104 94-95 0.2336865314619736 1104 96-97 0.34064838652009044 1104 98-99 0.19053248541865386 1104 100-101 0.40099385712102986 1105 1 0.19134657716826808 1105 2 0.2225125382591031 1105 3 0.20037987521154577 1105 4 0.1508433317216813 1105 5 0.14923366462130616 1105 6 0.24770967187747317 1105 7 0.1902231092906561 1105 8 0.1447062961079908 1105 9 0.18623935729858232 1105 10-11 0.2069781907926327 1105 12-13 0.1885075772719489 1105 14-15 0.2676597726778738 1105 16-17 0.28807608906829074 1105 18-19 0.1603609083095634 1105 20-21 0.2480675230695084 1105 22-23 0.16691725171276772 1105 24-25 0.2555055760753788 1105 26-27 0.25789790813751523 1105 28-29 0.273859844716533 1105 30-31 0.15685793832579265 1105 32-33 0.08096368373553986 1105 34-35 0.16874905703496523 1105 36-37 0.23430669615260769 1105 38-39 0.19979261974141593 1105 40-41 0.14339948687182869 1105 42-43 0.32268456395378564 1105 44-45 0.23123956064288365 1105 46-47 0.5234215992807876 1105 48-49 0.3370949661303655 1105 50-51 0.4660435485807781 1105 52-53 0.48808554497819046 1105 54-55 0.45013987354158047 1105 56-57 0.20505717007502255 1105 58-59 0.3524955322126928 1105 60-61 0.371996344433974 1105 62-63 0.43871377268772704 1105 64-65 0.31680124654486974 1105 66-67 0.27711392391518785 1105 68-69 0.2333657453142166 1105 70-71 0.16277362671578288 1105 72-73 0.21672277634578307 1105 74-75 0.2960572551252163 1105 76-77 0.10539982360334577 1105 78-79 0.4389985588956904 1105 80-81 0.4349588954184824 1105 82-83 0.28557746058320177 1105 84-85 0.17836975426355295 1105 86-87 0.2627978431567506 1105 88-89 0.2871568319223634 1105 90-91 0.13617155400162417 1105 92-93 0.44619900944549684 1105 94-95 0.5633506749830026 1105 96-97 0.5616513264090628 1105 98-99 0.42167547963550334 1105 100-101 0.6215783732576519 1106 1 0.29079861064057155 1106 2 0.24370865473647996 1106 3 0.21902807114937417 1106 4 0.19151985282405803 1106 5 0.2133703343361617 1106 6 0.21139229702274065 1106 7 0.19426122285663183 1106 8 0.2390642572763042 1106 9 0.24747258267767336 1106 10-11 0.28252743968227634 1106 12-13 0.3085340027720278 1106 14-15 0.40808150172629354 1106 16-17 0.290228688371748 1106 18-19 0.15454915550650128 1106 20-21 0.3979641381645891 1106 22-23 0.3270028958033748 1106 24-25 0.3307147779509876 1106 26-27 0.22864546324812096 1106 28-29 0.42461122038137233 1106 30-31 0.39559393625986417 1106 32-33 0.25479403697761427 1106 34-35 0.3105884593304822 1106 36-37 0.2988453200102015 1106 38-39 0.4507154781527376 1106 40-41 0.3243858619971931 1106 42-43 0.23452085320511884 1106 44-45 0.35839105446190445 1106 46-47 0.583738599155204 1106 48-49 0.4342820830799994 1106 50-51 0.5531676617611581 1106 52-53 0.5316893888802525 1106 54-55 0.3316469760580105 1106 56-57 0.3613244709065242 1106 58-59 0.45498587912255317 1106 60-61 0.28954135533847847 1106 62-63 0.3657115599500713 1106 64-65 0.2522038109150131 1106 66-67 0.443122753640516 1106 68-69 0.5323223448794465 1106 70-71 0.11356055476024096 1106 72-73 0.1585738525569944 1106 74-75 0.20061857236741432 1106 76-77 0.15867046565102072 1106 78-79 0.25114535661680293 1106 80-81 0.3960294819266217 1106 82-83 0.6133637677876003 1106 84-85 0.4201931491443638 1106 86-87 0.2099455164215378 1106 88-89 0.039262558685525306 1106 90-91 0.20487520103901247 1106 92-93 0.28685978844942994 1106 94-95 0.32499540589040166 1106 96-97 0.627322850721761 1106 98-99 0.8053798430268095 1106 100-101 1.093744928196692 1107 1 0.15147002085277705 1107 2 0.19901762951070623 1107 3 0.21821484216011555 1107 4 0.13886545250642257 1107 5 0.05637708744541925 1107 6 0.006512574809221405 1107 7 0.10461796395028955 1107 8 0.07205801961840308 1107 9 0.07323261221164046 1107 10-11 0.19936911601366347 1107 12-13 0.17516357783811287 1107 14-15 0.20507899760790593 1107 16-17 0.16589868805829866 1107 18-19 0.13239693715016188 1107 20-21 0.16033758266824094 1107 22-23 0.12936474265163866 1107 24-25 0.14633643665950302 1107 26-27 0.31611198503117066 1107 28-29 0.27727746214895177 1107 30-31 0.33165097722083203 1107 32-33 0.23463135635196863 1107 34-35 0.1816507418744422 1107 36-37 0.22567734904264825 1107 38-39 0.328158212552502 1107 40-41 0.3603185677336782 1107 42-43 0.36743416334314816 1107 44-45 0.4050228585068041 1107 46-47 0.29408106085333685 1107 48-49 0.19920318071275744 1107 50-51 0.1591286128385505 1107 52-53 0.07272349573889869 1107 54-55 0.08794993607222068 1107 56-57 0.12841795218832885 1107 58-59 0.06390632199278201 1107 60-61 0.10848384420228996 1107 62-63 0.08201653997632974 1107 64-65 -0.01361323171768447 1107 66-67 0.15153575555256538 1107 68-69 0.14014310015498666 1107 70-71 0.04501684413741458 1107 72-73 -0.022660409633605383 1107 74-75 -0.21514257165596007 1107 76-77 -0.09166649325566567 1107 78-79 0.1805912115869006 1107 80-81 0.24689599744066015 1107 82-83 -0.11425850389008829 1107 84-85 0.08565303274987812 1107 86-87 0.04647778263126412 1107 88-89 -0.09302646324553976 1107 90-91 -0.07906883766163375 1107 92-93 -0.01475935569236242 1107 94-95 0.2559588819828633 1107 96-97 0.19976553287983023 1107 98-99 0.3263975459944852 1107 100-101 0.6441111424503134 1108 1 0.05186299425375296 1108 2 -0.010335202602320237 1108 3 0.06366322611402353 1108 4 0.09088261821020893 1108 5 0.09249414529331546 1108 6 0.0789275364731381 1108 7 0.08584582661162443 1108 8 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-1.0458239842864465 2316 86-87 -0.9697174386266383 2316 88-89 -1.2956622716672683 2316 90-91 -1.363864723228975 2316 92-93 -1.4081510839683737 2316 94-95 -1.4905427787907364 2316 96-97 -1.3472075733025335 2316 98-99 -1.8169620382763796 2316 100-101 -1.7617953184947197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 19.0 6 44.0 7 105.0 8 276.0 9 540.0 10 941.0 11 1713.0 12 2744.0 13 3685.0 14 4784.0 15 6716.0 16 9538.0 17 13696.0 18 11947.0 19 6258.0 20 5194.0 21 5217.0 22 5404.0 23 5975.0 24 6921.0 25 8156.0 26 9662.0 27 12212.0 28 15261.0 29 19176.0 30 23767.0 31 30149.0 32 39320.0 33 51798.0 34 71294.0 35 104181.0 36 162690.0 37 241289.0 38 165069.0 39 10015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97265932933115 20.57239554908738 15.32673124734704 20.12821387423443 2 15.046575643505458 23.089754495113205 38.53189631164855 23.331773549732784 3 20.513410680237897 26.944020580376467 30.610752602620096 21.93181613676554 4 12.283805850549934 17.83523590499392 39.36381615588333 30.517142088572825 5 12.203886860864475 39.81285554888318 33.8738276240289 14.109429966223447 6 28.896704187678306 38.82505938827238 17.024069773169003 15.254166650880311 7 26.68380433505973 34.7695128835611 21.542787971554247 17.003894809824924 8 26.427307617990387 32.40818970906377 20.108565929506973 21.055936743438874 9 26.233987898810717 18.196301446156525 21.894855289629124 33.67485536540363 10-11 24.6637053793877 27.106483191900132 27.205240133305292 21.02457129540688 12-13 27.20106008539787 25.396963715375787 27.207974509454765 20.19400168977158 14-15 23.19031525984974 26.368392936300154 28.13119219825643 22.310099605593674 16-17 20.543748413471192 29.646398248093327 28.265076285988155 21.54477705244733 18-19 20.42515263856816 28.847552762838596 30.65345219850686 20.073842400086384 20-21 22.206398918690056 26.349162898831313 30.414494251278583 21.029943931200044 22-23 21.528396419385256 26.490553313053727 30.75950008548502 21.221550182075994 24-25 22.42458315946723 27.049555747433214 30.398678728119517 20.127182364980044 26-27 21.66419440150344 26.145577581764723 32.223939862386715 19.966288154345126 28-29 20.338362565012346 28.231294772206965 31.129062354074456 20.301280308706232 30-31 21.12726418516803 26.557377670301108 30.86206455609515 21.453293588435713 32-33 21.367438384379927 27.05380269456299 31.0265473229843 20.55221159807278 34-35 22.32293391326092 26.45292887425599 30.806600717584608 20.41753649489848 36-37 21.87783883425802 25.909634725212683 31.6624687047385 20.55005773579079 38-39 20.9418831505612 27.56634479256906 31.004968073885003 20.48680398298474 40-41 21.055413344573395 27.423460628606744 30.923682035714034 20.597443991105827 42-43 20.828092263043636 27.850400278850195 29.404346047033236 21.91716141107293 44-45 21.630733762469642 26.494604854872872 29.725251097783218 22.14941028487427 46-47 22.925763571714082 27.77538877133293 29.17063694279582 20.12821071415717 48-49 22.230762964562707 27.764169423068907 28.926925976865164 21.07814163550322 50-51 20.582544780442152 28.08265016290471 30.19266755929954 21.1421374973536 52-53 21.927296251813853 29.000467907600562 27.872659041225123 21.199576799360464 54-55 21.112913965936464 27.890432524132525 28.59472991750755 22.401923592423465 56-57 22.184912474963923 27.855415204600813 28.188029759601562 21.7716425608337 58-59 23.351249311515907 27.241190363543282 28.093513544488978 21.314046780451832 60-61 21.972770719264286 28.236158859414523 29.601032831520307 20.190037589800884 62-63 21.234809156256055 28.072494526110088 29.4850730822914 21.207623235342453 64-65 21.06892310635857 29.26109872144661 28.44851143272144 21.221466739473378 66-67 21.901287915456216 28.018294328985345 27.5949881457219 22.48542960983654 68-69 21.336792457744835 27.96543906584697 28.5261733625635 22.171595113844692 70-71 22.684041975806796 28.202435991062284 27.930544228705443 21.182977804425477 72-73 22.1975744579281 27.241741525568212 29.324640734103458 21.23604328240023 74-75 21.307555476413288 30.043930272295704 27.547491674275694 21.101022577015318 76-77 21.699593848625263 28.216107008763323 27.865649899030466 22.218649243580952 78-79 21.862202455234197 28.42761671976506 27.971729902452726 21.738450922548022 80-81 22.531357092926115 28.56464931773104 27.535894186268994 21.368099403073856 82-83 22.007788758118423 28.103188256424637 28.593372261335475 21.29565072412147 84-85 21.42351373739967 28.76501466121487 27.913854291537227 21.897617309848236 86-87 21.12927937634831 28.953200074830626 27.806065543102203 22.11145500571886 88-89 21.159683167379026 28.795396691964335 27.014882608541217 23.030037532115415 90-91 21.679152950224886 27.598830603779177 27.813983974554784 22.908032471441146 92-93 22.397650986846 28.513941345709853 27.936586116669233 21.151821550774912 94-95 21.65409203860524 27.947168092096387 28.10525273889465 22.29348713040373 96-97 20.56452586845853 28.160036296970215 29.09881310233482 22.176624732236437 98-99 21.219652889008024 29.030597408649644 27.032383828261235 22.717365874081096 100-101 20.529249322407235 28.35650543518671 27.71213519745738 23.402110044948685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 873.0 1 594.0 2 341.5 3 381.0 4 369.0 5 371.0 6 569.0 7 1132.5 8 1872.5 9 2229.5 10 2019.5 11 1748.0 12 1750.5 13 1814.5 14 1900.5 15 1997.0 16 2038.5 17 2128.0 18 2201.0 19 2273.0 20 2521.5 21 2919.0 22 3326.5 23 3772.0 24 4297.0 25 5240.5 26 6900.5 27 8284.5 28 8929.0 29 10058.5 30 12016.0 31 13858.5 32 16627.5 33 20718.0 34 25231.0 35 28386.5 36 33108.5 37 37774.5 38 37995.0 39 36693.0 40 34450.5 41 32911.5 42 31882.5 43 31799.5 44 33304.5 45 33971.5 46 33772.0 47 43044.5 48 64935.0 49 61874.0 50 38950.0 51 29457.0 52 26655.5 53 25632.5 54 24572.5 55 22929.5 56 21377.5 57 19526.0 58 18003.5 59 16545.0 60 14336.5 61 12003.0 62 9694.0 63 7462.5 64 5514.5 65 4114.5 66 3025.0 67 2355.0 68 1676.0 69 970.0 70 644.5 71 462.5 72 334.0 73 250.5 74 169.5 75 117.0 76 89.5 77 58.0 78 26.5 79 12.5 80 9.5 81 6.5 82 4.5 83 3.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032204166840316586 2 0.005872524541469496 3 9.471813776563702E-5 4 0.0 5 1.8943627553127403E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013449975562720455 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.8943627553127403E-4 20-21 7.103860332422777E-4 22-23 0.0022258762374924696 24-25 0.010845226774165438 26-27 0.004925343163813125 28-29 2.841544132969111E-4 30-31 0.002936262270734748 32-33 0.004309675268336484 34-35 0.0 36-37 0.008808786812204244 38-39 0.011697690014056171 40-41 0.0015628492731330108 42-43 0.0 44-45 0.0 46-47 1.8943627553127403E-4 48-49 0.0037887255106254804 50-51 0.008098400778961965 52-53 0.0 54-55 0.002936262270734748 56-57 0.0035519301662113884 58-59 2.3679534441409253E-4 60-61 0.002415312513023744 62-63 0.00667762871247741 64-65 4.735906888281851E-5 66-67 0.002557389719672199 68-69 5.209497577110036E-4 70-71 0.0012313357909532812 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018470036864299219 80-81 0.0020837990308440143 82-83 0.013260539287189184 84-85 0.008287837054493239 86-87 0.004404393406102122 88-89 1.4207720664845554E-4 90-91 5.209497577110036E-4 92-93 1.4207720664845554E-4 94-95 4.735906888281851E-5 96-97 0.002699466926320655 98-99 0.0019890808930783776 100-101 4.2623161994536653E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1055764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.864470344144465 #Duplication Level Percentage of deduplicated Percentage of total 1 72.72942268962122 28.993199139571523 2 13.943519546300456 11.117020428929864 3 5.121534885816552 6.125018267164058 4 2.3989998685114915 3.8253943645553132 5 1.3423907469043073 2.6756848060110348 6 0.8730703787628299 2.0882692933525084 7 0.5825980957428587 1.6257475157207355 8 0.41679040464292016 1.329210298048933 9 0.30813295691254816 1.1055201410604438 >10 1.9239014838278414 14.974144005853459 >50 0.21526037555737246 6.011309930665718 >100 0.13244645440557676 9.716932931139818 >500 0.005727414237150539 1.525370104782701 >1k 0.005011487457506721 4.852092519286024 >5k 9.545690395250897E-4 2.489452719343503 >10k+ 2.3864225988127244E-4 1.545633534514441 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16247 1.5388855842783047 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7202 0.6821600281881178 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6848 0.6486298074190824 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6564 0.6217298562936414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 0.5260645371503481 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4436 0.4201696591283658 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4276 0.4050147570858639 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4175 0.39544822517153455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3672 0.34780500187541913 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3276 0.3102966193202269 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3245 0.3073603570494921 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3242 0.3070762026361952 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3180 0.3012036780947257 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2586 0.24494110426193733 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2576 0.24399392288428096 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2145 0.20317040550729143 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1766 0.16727223129411498 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1624 0.15382225573139452 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1591 0.1506965571851285 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1482 0.14037228016867406 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1464 0.13866735368889258 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1439 0.13629940024475168 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.11659802758949916 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1212 0.11479838297195208 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1206 0.11423007414535825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1179 0.11167268442568604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 9.471813776563702E-5 0.0 0.0 0.0 5 0.0 1.8943627553127403E-4 0.0 0.0 0.0 6 0.0 1.8943627553127403E-4 0.0 0.0 0.0 7 0.0 1.8943627553127403E-4 0.0 0.0 0.0 8 0.0 1.8943627553127403E-4 0.0 0.0 0.0 9 0.0 1.8943627553127403E-4 0.0 0.0 0.0 10-11 0.0 1.8943627553127403E-4 0.0 0.0 0.0 12-13 0.0 1.8943627553127403E-4 0.0 0.0 0.0 14-15 0.0 1.8943627553127403E-4 0.0 0.0 0.0 16-17 0.0 1.8943627553127403E-4 0.0 0.0 0.0 18-19 0.0 1.8943627553127403E-4 0.0 4.735906888281851E-5 0.0 20-21 0.0 1.8943627553127403E-4 0.0 3.315134821797296E-4 0.0 22-23 0.0 1.8943627553127403E-4 0.0 9.471813776563702E-4 0.0 24-25 0.0 1.8943627553127403E-4 0.0 0.0016102083420158292 0.0 26-27 0.0 1.8943627553127403E-4 0.0 0.002178517168609651 0.0 28-29 0.0 1.8943627553127403E-4 0.0 0.0041202389928052105 0.0 30-31 0.0 1.8943627553127403E-4 0.0 0.007009142194657139 0.0 32-33 0.0 1.8943627553127403E-4 0.0 0.010040122603157524 0.0 34-35 0.0 1.8943627553127403E-4 0.0 0.012550153253946905 0.0 36-37 0.0 1.8943627553127403E-4 0.0 0.015960006213509837 0.0 38-39 0.0 1.8943627553127403E-4 0.0 0.019133063828658675 0.0 40-41 0.0 2.841544132969111E-4 0.0 0.026379001367729908 0.0 42-43 0.0 2.841544132969111E-4 0.0 0.03627704676423897 0.0 44-45 0.0 3.7887255106254806E-4 0.0 0.056641446383850935 0.0 46-47 0.0 5.209497577110037E-4 0.0 0.1003065078938096 0.0 48-49 0.0 5.683088265938222E-4 0.0 0.12853251294796944 0.0 50-51 0.0 5.683088265938222E-4 0.0 0.16462012343667715 0.0 52-53 0.0 5.683088265938222E-4 0.0 0.20250737854293194 0.0 54-55 0.0 5.683088265938222E-4 0.0 0.24584092657071088 0.0 56-57 0.0 5.683088265938222E-4 0.0 0.29528379448437336 0.0 58-59 0.0 5.683088265938222E-4 0.0 0.3576556882030454 0.0 60-61 0.0 5.683088265938222E-4 0.0 0.44138652198786854 0.0 62-63 0.0 5.683088265938222E-4 0.0 0.5487021720763352 0.0 64-65 0.0 5.683088265938222E-4 0.0 0.624145168806665 0.0 66-67 0.0 5.683088265938222E-4 0.0 0.7432058679780709 0.0 68-69 0.0 6.156678954766406E-4 0.0 0.897122841847231 0.0 70-71 0.0 6.630269643594591E-4 0.0 0.996813681845564 0.0 72-73 0.0 6.630269643594591E-4 0.0 1.1707161827832735 0.0 74-75 0.0 6.630269643594591E-4 0.0 1.3183817595599017 0.0 76-77 0.0 6.630269643594591E-4 0.0 1.4324223974297285 0.0 78-79 0.0 6.630269643594591E-4 0.0 1.5938221041823741 0.0 80-81 0.0 6.630269643594591E-4 0.0 1.7444239432297368 0.0 82-83 0.0 7.577451021250961E-4 0.0 1.9326762420389405 0.0 84-85 0.0 8.524632398907332E-4 0.0 2.2083533820058268 0.0 86-87 0.0 9.471813776563702E-4 0.0 2.5897359637191646 0.0 88-89 0.0 9.471813776563702E-4 0.0 3.0638476023050605 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 205 0.0 32.43658 2 TAATACC 310 0.0 30.639954 4 TTTGCGT 220 0.0 30.222134 3 CATATAG 270 0.0 26.384401 3 GTACATG 6680 0.0 24.395067 1 ATACCTT 405 0.0 23.459469 6 ACATGGG 6690 0.0 23.071564 3 TACATGG 7180 0.0 22.425192 2 CATTCCG 300 7.2759576E-12 22.169199 9 GAGTACT 5445 0.0 22.110565 12-13 AGTACTT 5785 0.0 21.91935 12-13 TTGCGTT 330 1.8189894E-12 21.58724 4 CATGGGG 1970 0.0 20.97359 4 ATAACCC 500 0.0 20.896446 3 GTACTTT 5775 0.0 20.641512 14-15 AATACCT 485 0.0 20.569359 5 CATGGGA 4575 0.0 20.553885 4 AGAGTAC 9955 0.0 20.30493 10-11 TTAGGCA 410 0.0 19.691772 4 CGGTATA 230 0.0 19.624596 34-35 >>END_MODULE