FastQCFastQC Report
Thu 26 May 2016
SRR937080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070252
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127871.194765344984172No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88870.8303651850218453No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT72000.6727387568535261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71460.6676932161771246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51780.4838112893038275No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50580.47259897668960205No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41280.38570355392935496No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40110.37477154913048516No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35000.3270257845815752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.32226055172052936No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33160.3098335719064295No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32850.30693705781442127No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26870.2510623666201979No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26110.24396123529785507No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC20740.19378613634919625No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20340.19004869881112113No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19490.18210664404271143No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18640.17416458927430176No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC17220.16089668601413498No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC16800.15697237659915608No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC15800.14762878275396824No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15700.14669442336944943No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG12170.11371153709593627No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.11100189488083181No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT11690.1092266120502461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG2800.027.1658763
GTACATG61600.026.8534071
TACATGG64850.025.5100172
ACATGGG62050.025.4365443
GAGTACT50800.024.4634312-13
CATGGGA43050.023.5216774
GGATATG5000.022.8161351
ATGGGAT17700.022.5614935
CATTCCG2554.8748916E-1022.3645749
GTACTTT55750.022.24820114-15
TAATACC4500.022.1854674
GTATAGG4100.022.0277731
CTACACT2606.184564E-1021.9416714
AGTACTT55550.021.77274112-13
CATGGGG17500.020.9177274
AGAGTAC91150.020.85365910-11
GATTGGC5250.020.8203549
AGGCTTA4350.020.762786
GTTATAT2308.9519745E-820.6667881
AGGGACG1200.00473998319.8084535
Produced by FastQC (version 0.11.5)