FastQCFastQC Report
Thu 26 May 2016
SRR937079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314708
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116720.8878017019748872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82310.626070579931057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56100.42671072207668925No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24590.1870377300510836No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21400.16277378703103654No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20730.15767759837165363No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.1385098440109895No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.13288121773047704No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17350.13196846752282637No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15320.11652777651006915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.10671571177782442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT500.001611932638.019026
GTATCAA133650.028.8066771
GTACATG85550.026.0017721
GGTATCA93750.024.9442771
GAGTACT76250.024.6453412-13
TACATGG88850.024.6080672
ATCAACG160150.023.9194983
TATCAAC160100.023.8991052
TCAACGC160200.023.8536074
ACATGGG87450.023.8044573
CAACGCA162350.023.476485
AACGCAG164650.023.2928566
AGTACTT79150.022.81186912-13
GTACTTT81850.022.75641414-15
TGGACCG1903.2718526E-722.5112615
GTCGGGA4450.022.4303672
CATGGGG31100.021.548614
ACGCAGA177400.021.5107867
CGCAGAG177900.021.3960888
CATGGGA56000.021.3881424
Produced by FastQC (version 0.11.5)