##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1314708 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824369365669032 33.0 31.0 34.0 30.0 34.0 2 31.90852645606477 33.0 31.0 34.0 30.0 34.0 3 31.902390492793838 33.0 31.0 34.0 30.0 34.0 4 35.50096447271942 37.0 35.0 37.0 33.0 37.0 5 35.532857486225076 37.0 35.0 37.0 33.0 37.0 6 35.608634008464236 37.0 35.0 37.0 33.0 37.0 7 35.54217514459484 37.0 35.0 37.0 33.0 37.0 8 35.51934878315185 37.0 35.0 37.0 33.0 37.0 9 37.13654514918902 39.0 37.0 39.0 33.0 39.0 10-11 37.00481703922088 39.0 37.0 39.0 33.0 39.0 12-13 36.897017056258875 39.0 37.0 39.0 33.0 39.0 14-15 38.014115681961314 40.0 38.0 41.0 33.0 41.0 16-17 37.96639481922982 40.0 38.0 41.0 33.0 41.0 18-19 37.972404899034615 40.0 38.0 41.0 33.0 41.0 20-21 37.94131244352358 40.0 38.0 41.0 33.0 41.0 22-23 37.80499167876061 40.0 37.5 41.0 33.0 41.0 24-25 37.62799191911816 40.0 37.0 41.0 32.0 41.0 26-27 37.570220915975256 40.0 37.0 41.0 32.0 41.0 28-29 37.61838750505816 40.0 37.0 41.0 32.0 41.0 30-31 37.44799947973238 40.0 37.0 41.0 32.0 41.0 32-33 37.21067910136699 39.5 36.0 41.0 31.5 41.0 34-35 36.99582150561189 39.0 36.0 41.0 31.0 41.0 36-37 36.81849049370659 39.0 35.0 41.0 31.0 41.0 38-39 36.628749121477924 39.0 35.0 41.0 30.5 41.0 40-41 36.38508588979454 39.0 35.0 41.0 30.0 41.0 42-43 36.13441235620381 39.0 35.0 41.0 30.0 41.0 44-45 35.81669085454717 39.0 35.0 40.0 28.5 41.0 46-47 35.35037894346121 38.5 35.0 40.0 26.5 41.0 48-49 34.92377775140944 38.0 34.5 40.0 24.5 41.0 50-51 34.22699679320427 37.5 33.5 39.5 24.0 40.5 52-53 34.48998674990949 38.0 34.0 40.0 24.0 40.5 54-55 34.77168276149533 38.0 35.0 40.0 24.5 41.0 56-57 34.64561180125168 38.0 34.5 40.0 24.0 41.0 58-59 34.40459592548307 38.0 34.0 40.0 23.0 41.0 60-61 34.11351494019965 38.0 34.0 40.0 23.0 41.0 62-63 33.785316587409525 37.0 34.0 40.0 22.0 41.0 64-65 33.46118605804483 37.0 33.0 40.0 21.5 41.0 66-67 33.08936623189332 36.0 33.0 39.0 20.0 41.0 68-69 32.663694523803 36.0 33.0 39.0 18.0 41.0 70-71 32.30344342622088 35.0 32.0 38.5 18.0 40.0 72-73 31.928118639271993 35.0 32.0 37.5 15.0 40.0 74-75 31.514818879933795 35.0 31.5 37.0 11.5 39.0 76-77 31.083766129056794 35.0 31.0 36.5 7.5 39.0 78-79 30.66737062526432 35.0 31.0 36.0 6.5 39.0 80-81 30.261198684422702 35.0 31.0 36.0 2.0 37.5 82-83 29.87578914861703 34.0 30.0 35.0 2.0 37.0 84-85 29.54637113336193 34.0 30.0 35.0 2.0 36.5 86-87 29.21704325218984 34.0 29.5 35.0 2.0 36.0 88-89 28.855087213282342 34.0 29.0 35.0 2.0 36.0 90-91 28.528092549828557 34.0 29.0 35.0 2.0 35.5 92-93 28.18626341362493 34.0 29.0 35.0 2.0 35.0 94-95 27.807142726749973 34.0 28.0 35.0 2.0 35.0 96-97 27.380134600230623 34.0 27.0 35.0 2.0 35.0 98-99 26.800369739896617 34.0 25.5 35.0 2.0 35.0 100-101 24.69719549892448 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.567685964926369 1101 2 0.41847375664183417 1101 3 0.6148092115666586 1101 4 0.4533577945808105 1101 5 0.3778840570658204 1101 6 0.28371147300218524 1101 7 0.40910342082251816 1101 8 0.42054084313450346 1101 9 0.5502640039833437 1101 10-11 0.5645640437839958 1101 12-13 0.6748842450507411 1101 14-15 0.8872112625433459 1101 16-17 0.9351708899457449 1101 18-19 0.8278238494737096 1101 20-21 0.762406608313249 1101 22-23 0.9345233321468172 1101 24-25 1.0139240129848588 1101 26-27 1.0298908336128108 1101 28-29 1.0746661871839862 1101 30-31 0.8940107769461321 1101 32-33 0.6964213464192994 1101 34-35 0.8692472815619325 1101 36-37 0.9356150681416011 1101 38-39 0.9675198433687697 1101 40-41 1.001502156907904 1101 42-43 1.053556764146272 1101 44-45 1.1554320567592171 1101 46-47 1.127054778986313 1101 48-49 1.0751368224091422 1101 50-51 1.146289674185681 1101 52-53 1.1233237613097131 1101 54-55 1.1824464325795745 1101 56-57 1.1111914955038316 1101 58-59 1.0196338823809583 1101 60-61 1.1820417687247158 1101 62-63 1.2206193916734733 1101 64-65 1.0736782675360033 1101 66-67 0.8741450150529388 1101 68-69 0.9365219173120423 1101 70-71 1.038289917487063 1101 72-73 1.007948318099654 1101 74-75 0.8005470181529724 1101 76-77 0.8902593286475486 1101 78-79 1.0911767138328408 1101 80-81 1.136222460912066 1101 82-83 0.8991719144114718 1101 84-85 1.0881141955326328 1101 86-87 1.2168825568886419 1101 88-89 1.084069591968845 1101 90-91 1.057297414250403 1101 92-93 0.9001984978023927 1101 94-95 1.170465126229388 1101 96-97 1.18670064089412 1101 98-99 1.2887141882044801 1101 100-101 1.5315032284977335 1102 1 0.30587669664136996 1102 2 0.28575543502738654 1102 3 0.2746390974748678 1102 4 0.1751881844141181 1102 5 0.14666609371062123 1102 6 0.18942285383570834 1102 7 0.07684601351717646 1102 8 0.1788733234696167 1102 9 0.27296740469041225 1102 10-11 0.327376487190854 1102 12-13 0.22631954579166091 1102 14-15 0.5039560441626776 1102 16-17 0.2766510124625299 1102 18-19 0.1736788686464834 1102 20-21 0.11505969524819903 1102 22-23 0.2752861979846273 1102 24-25 0.3067177855729071 1102 26-27 0.03819181862890275 1102 28-29 0.2180067972483144 1102 30-31 0.23992337349896786 1102 32-33 0.31281574261466716 1102 34-35 0.1084589045157145 1102 36-37 0.1008567356612673 1102 38-39 -0.04242726417987086 1102 40-41 0.04518739465819266 1102 42-43 0.13367002918725746 1102 44-45 0.34537442032543453 1102 46-47 0.31245984815790706 1102 48-49 0.5312840197194433 1102 50-51 0.4455810787194281 1102 52-53 0.544259191968834 1102 54-55 0.7034673435029362 1102 56-57 0.9959319150214796 1102 58-59 0.7101995087834894 1102 60-61 0.7897892235092527 1102 62-63 0.8123298237105132 1102 64-65 0.9477658875134907 1102 66-67 0.9180384171773 1102 68-69 0.6889290947015567 1102 70-71 0.7595927607269886 1102 72-73 0.8786920210043085 1102 74-75 0.8967952366989955 1102 76-77 0.888511830556947 1102 78-79 0.9868145137273281 1102 80-81 0.9060837043472603 1102 82-83 0.861882440934469 1102 84-85 0.942049240374093 1102 86-87 0.9415130774275227 1102 88-89 0.8458911631128352 1102 90-91 0.716577854476224 1102 92-93 0.6858959813264072 1102 94-95 0.7614157336953546 1102 96-97 0.6472347916622105 1102 98-99 0.5386280681915672 1102 100-101 0.8789935436871374 1103 1 0.352805657193926 1103 2 0.295667963198337 1103 3 0.2871295720439875 1103 4 0.283407212576968 1103 5 0.30611167310002685 1103 6 0.28757228555255665 1103 7 0.2591475602850153 1103 8 0.2792429912550105 1103 9 0.26462555926991627 1103 10-11 0.3108463179219143 1103 12-13 0.08132931442502667 1103 14-15 0.38000282821507625 1103 16-17 0.5429090989453371 1103 18-19 0.574673161494708 1103 20-21 0.5486735221204597 1103 22-23 0.40833265371366423 1103 24-25 0.7502086735862648 1103 26-27 0.7336293399091289 1103 28-29 0.7080952824210769 1103 30-31 0.811581973949707 1103 32-33 0.6574772452817896 1103 34-35 0.6495819122819881 1103 36-37 0.7786813299184487 1103 38-39 0.8525001398767458 1103 40-41 0.9733681792294249 1103 42-43 0.8958686607679098 1103 44-45 0.8449690828414376 1103 46-47 0.843670834425069 1103 48-49 0.9840348425545358 1103 50-51 0.8131558026846051 1103 52-53 0.8164200055622288 1103 54-55 0.8242898340585754 1103 56-57 0.8787924221797425 1103 58-59 0.9714096235750631 1103 60-61 0.8395038659205198 1103 62-63 0.9206968000550191 1103 64-65 0.8779667727705558 1103 66-67 0.7118488124624207 1103 68-69 0.7553147611574431 1103 70-71 0.7695417692714983 1103 72-73 0.8096559540749872 1103 74-75 0.7651588487423773 1103 76-77 0.7525667051455329 1103 78-79 0.723672459152688 1103 80-81 0.7188532537741139 1103 82-83 0.6461395383938147 1103 84-85 0.6774945846677554 1103 86-87 0.810876859712657 1103 88-89 0.7505416000666401 1103 90-91 0.7638700987488072 1103 92-93 0.948217227495963 1103 94-95 0.8156215154292248 1103 96-97 0.7513533245026558 1103 98-99 0.8891162971910305 1103 100-101 1.1745707037305912 1104 1 0.3592671917204484 1104 2 0.3982041927655864 1104 3 0.3503179018497491 1104 4 0.44097785463801387 1104 5 0.4154099736834027 1104 6 0.4761067119008686 1104 7 0.4709394611463438 1104 8 0.41664571654548155 1104 9 0.49843335659322463 1104 10-11 0.5549331026290858 1104 12-13 0.5836243687880298 1104 14-15 0.6857965749246233 1104 16-17 0.7168158175344743 1104 18-19 0.5691633258519389 1104 20-21 0.5703814219672054 1104 22-23 0.6104173325339488 1104 24-25 0.5975030991672554 1104 26-27 0.6929641951080896 1104 28-29 0.6551790127396586 1104 30-31 0.6080201902549831 1104 32-33 0.6648926101565351 1104 34-35 0.6359943591818791 1104 36-37 0.6225185201367296 1104 38-39 0.5954071590023844 1104 40-41 0.5449949768532676 1104 42-43 0.6810639348848966 1104 44-45 0.5989053648427145 1104 46-47 0.6886568839901415 1104 48-49 0.7699702677071016 1104 50-51 0.8419913197032827 1104 52-53 0.9671395879657112 1104 54-55 0.868110079134361 1104 56-57 0.8550457765719415 1104 58-59 0.8979396420548582 1104 60-61 0.8734655549782175 1104 62-63 0.8286900049463028 1104 64-65 0.8682760695791103 1104 66-67 0.9506607554841082 1104 68-69 1.1314335135686662 1104 70-71 1.0834334823508058 1104 72-73 1.0751687524533686 1104 74-75 0.8849130482602483 1104 76-77 1.0289457093679637 1104 78-79 0.8604629212755022 1104 80-81 0.9081614017233264 1104 82-83 0.7768638526484075 1104 84-85 0.8423622953257137 1104 86-87 0.9841873011176325 1104 88-89 1.039356633711808 1104 90-91 0.8853427914918051 1104 92-93 0.6753892430727078 1104 94-95 0.9125866297503222 1104 96-97 0.8006706164344237 1104 98-99 0.8889228667908071 1104 100-101 1.0238255437535244 1105 1 0.32473743771038954 1105 2 0.26849199402701274 1105 3 0.37611354501868277 1105 4 0.2707817869585014 1105 5 0.28247624691239537 1105 6 0.3495075145411519 1105 7 0.36269212090614644 1105 8 0.30916434600235476 1105 9 0.42042953873978917 1105 10-11 0.5359018115388992 1105 12-13 0.3623496604105725 1105 14-15 0.47274161816964977 1105 16-17 0.6185612117204187 1105 18-19 0.5049755221915078 1105 20-21 0.5159130369237772 1105 22-23 0.5500188226620182 1105 24-25 0.6427552865505106 1105 26-27 0.6755368137191482 1105 28-29 0.5460974452492238 1105 30-31 0.5461525722094009 1105 32-33 0.47340332744916935 1105 34-35 0.432899007664048 1105 36-37 0.5380265219782601 1105 38-39 0.35768038113587863 1105 40-41 0.3313145638579442 1105 42-43 0.3354316757369631 1105 44-45 0.45552023573598177 1105 46-47 0.6067541033088091 1105 48-49 0.5731217674883311 1105 50-51 0.3316695781589303 1105 52-53 0.3452147478140475 1105 54-55 0.4179777908555877 1105 56-57 0.4904459143436668 1105 58-59 0.619394276200083 1105 60-61 0.5885739502574197 1105 62-63 0.5309616962827945 1105 64-65 0.6250841179157405 1105 66-67 0.7756823751358866 1105 68-69 0.7463512446533329 1105 70-71 0.5844676271207874 1105 72-73 0.5402582983664281 1105 74-75 0.5107042932437444 1105 76-77 0.39181885541856687 1105 78-79 0.3724405510762345 1105 80-81 0.5169502328455735 1105 82-83 0.7171847602511043 1105 84-85 0.5846260585045613 1105 86-87 0.8134531697135614 1105 88-89 0.6297630488936576 1105 90-91 0.7688871234874064 1105 92-93 0.8792268597816992 1105 94-95 0.920357138146283 1105 96-97 0.9600402936304597 1105 98-99 1.0125004536378341 1105 100-101 1.0117074004960571 1106 1 0.6426641726621938 1106 2 0.50980409806618 1106 3 0.5369271998903997 1106 4 0.44764541340053654 1106 5 0.4330606049270074 1106 6 0.5495386389823267 1106 7 0.6743360303515402 1106 8 0.5977943585868672 1106 9 0.6888151907766229 1106 10-11 0.7146315570730124 1106 12-13 0.6554415165860092 1106 14-15 0.9274942933894721 1106 16-17 0.7395224449489248 1106 18-19 0.756632123941479 1106 20-21 0.8343174175204524 1106 22-23 0.9239900992491599 1106 24-25 0.914851618717023 1106 26-27 0.9887898298257198 1106 28-29 0.9055753091693859 1106 30-31 0.9013252252783204 1106 32-33 0.763793016231709 1106 34-35 0.6848166639801505 1106 36-37 0.907117257134864 1106 38-39 0.8897190185590418 1106 40-41 1.0026549493698482 1106 42-43 0.9421106978636118 1106 44-45 0.9711525200004871 1106 46-47 0.5643928630739268 1106 48-49 0.6966399880865097 1106 50-51 0.8895468912708111 1106 52-53 0.9550493301671636 1106 54-55 0.9257750696901539 1106 56-57 0.9151490195119436 1106 58-59 0.9168351330249607 1106 60-61 0.818985089304185 1106 62-63 0.9147796142597784 1106 64-65 0.8821901916281192 1106 66-67 0.8618549716149175 1106 68-69 0.8892983250190625 1106 70-71 0.7234625097776046 1106 72-73 0.6014414043970255 1106 74-75 0.8422230447090193 1106 76-77 0.8524329635483774 1106 78-79 1.0960592187345704 1106 80-81 1.2122365642305937 1106 82-83 1.2634405977680423 1106 84-85 1.0966733056757434 1106 86-87 1.0625002363360352 1106 88-89 1.1503189906976878 1106 90-91 1.1590633201615397 1106 92-93 1.317871963004361 1106 94-95 1.4855965268676492 1106 96-97 1.329086228455072 1106 98-99 1.370678434240972 1106 100-101 1.6842361737619136 1107 1 0.21448743064040343 1107 2 0.24029134899819482 1107 3 0.27459220656545114 1107 4 0.3353965586839607 1107 5 0.2520255013667949 1107 6 0.24154879835259635 1107 7 0.3172277948358442 1107 8 0.3843090963211182 1107 9 0.4087456151546789 1107 10-11 0.43403711758330843 1107 12-13 0.3678396597589071 1107 14-15 0.23984299687877808 1107 16-17 0.49193834209222587 1107 18-19 0.40906006941103357 1107 20-21 0.43982613386130964 1107 22-23 0.6678641485392802 1107 24-25 0.5502457236233198 1107 26-27 0.5394779692871623 1107 28-29 0.43576259832368436 1107 30-31 0.4630202724468262 1107 32-33 0.4616597700525418 1107 34-35 0.7174752448812001 1107 36-37 0.8154239548963673 1107 38-39 0.7631395799279517 1107 40-41 0.6108329931648271 1107 42-43 0.5525599204003484 1107 44-45 0.6691494511394538 1107 46-47 0.7538015533793185 1107 48-49 0.6117186761207236 1107 50-51 0.4909564069557959 1107 52-53 0.5873240264662556 1107 54-55 0.8380660243053626 1107 56-57 0.6748084534898879 1107 58-59 0.5406358398601014 1107 60-61 0.8235551440894469 1107 62-63 0.7288774023532696 1107 64-65 0.9006338248493719 1107 66-67 0.7690073843268692 1107 68-69 0.7943125060375493 1107 70-71 0.8645636437051394 1107 72-73 0.8828016742795306 1107 74-75 1.0327174692421757 1107 76-77 1.0223351366786666 1107 78-79 1.0733204264477152 1107 80-81 0.7659859479656319 1107 82-83 0.7586219596997665 1107 84-85 0.72035906870871 1107 86-87 0.7581338952049848 1107 88-89 0.6366482949968635 1107 90-91 0.57499577752154 1107 92-93 0.7048655870855036 1107 94-95 0.7716334861470777 1107 96-97 0.9783823015316315 1107 98-99 1.0734981580434813 1107 100-101 1.4254166604588967 1108 1 0.1539212083094732 1108 2 0.29518067271105153 1108 3 0.24991567483009192 1108 4 0.275814804984563 1108 5 0.30806194505029794 1108 6 0.23809398607425436 1108 7 0.18933737047483135 1108 8 0.07539017740219833 1108 9 0.16576775041210823 1108 10-11 0.08138577846137451 1108 12-13 -0.004866711770993959 1108 14-15 0.3108164590287146 1108 16-17 0.3926060686423085 1108 18-19 0.40723115905270646 1108 20-21 0.33025416370109895 1108 22-23 0.38382660420761994 1108 24-25 0.348197906575507 1108 26-27 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-2.1647988546014716 2316 84-85 -1.9845344269236236 2316 86-87 -1.9493133512434184 2316 88-89 -2.3711457327804304 2316 90-91 -2.2644484098760493 2316 92-93 -2.6590866648188687 2316 94-95 -2.638976828118789 2316 96-97 -2.5794468624244438 2316 98-99 -2.5938210790723844 2316 100-101 -2.1899245141563135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3005.0 3 853.0 4 834.0 5 900.0 6 1052.0 7 1196.0 8 1578.0 9 2018.0 10 2482.0 11 3550.0 12 4955.0 13 6361.0 14 7180.0 15 9228.0 16 12363.0 17 16734.0 18 13770.0 19 8485.0 20 7738.0 21 8017.0 22 8450.0 23 9239.0 24 10552.0 25 12094.0 26 14062.0 27 16455.0 28 19523.0 29 23736.0 30 29086.0 31 36109.0 32 46735.0 33 61517.0 34 85225.0 35 123617.0 36 183835.0 37 257820.0 38 225103.0 39 39241.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53169832516185 19.01336308658531 15.007481441003168 21.447457147249676 2 15.280594842183485 21.23046327766205 40.326276967681416 23.162664912473048 3 18.33425559235289 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22-23 21.870045070589786 25.9243809739845 31.24257355275147 20.963000402674243 24-25 21.585021778989116 26.24925697228288 31.322052758202606 20.843668490525395 26-27 21.332113621903666 26.305240903154708 31.397967384388707 20.96467809055292 28-29 20.95210041626992 26.238553338341664 31.733712078830212 21.075634166558206 30-31 21.104820552939174 26.38498292112822 31.473371837688124 21.036824688244476 32-33 20.990677227984982 26.538793895621936 31.25485858312586 21.215670293267216 34-35 21.487362434156356 26.317557746834712 30.976114414602257 21.218965404406674 36-37 21.63079399325354 26.176987075944286 30.78459463213149 21.407624298670687 38-39 21.42583995315234 26.24876669128528 31.115641645862606 21.20975170969977 40-41 21.169311027614988 26.377657442895163 31.234779749488805 21.218251780001044 42-43 21.16417237943813 26.726484908312777 30.45733630978431 21.65200640246478 44-45 21.198854385415522 26.962618369329434 30.047498029952337 21.791029215302714 46-47 21.5190056421514 27.0540969323893 29.547600658948497 21.879296766510805 48-49 21.820518000973284 27.19099892360568 29.007209825360825 21.981273250060212 50-51 21.772410778583893 27.45253564843188 28.870179715345134 21.90487385763909 52-53 21.755983679283617 27.751685870344357 28.71526863588701 21.777061814485016 54-55 21.48946111593914 27.538684682737657 28.820374009007022 22.151480192316182 56-57 21.640502734095122 27.942981055960768 28.333489973208874 22.083026236735233 58-59 21.81955727827858 27.262345593274585 28.747496557111983 22.17060057133485 60-61 21.904961589803147 27.243309228340895 29.104685480839045 21.747043701016917 62-63 21.825357562546806 27.319171380383228 29.140243976692222 21.715227080377748 64-65 21.808690755656677 27.420699993531965 29.022223405914826 21.748385844896532 66-67 21.549012981635734 27.73226289639047 28.76388279994155 21.954841322032248 68-69 21.30190393709656 27.771469710368386 29.011220580602643 21.915405771932413 70-71 21.675913132923913 27.66206169315325 28.663639628234233 21.9983855456886 72-73 21.524057610911427 27.64564488943378 28.434650993561128 22.395646506093666 74-75 21.339693515216577 28.198700015140876 28.25542123207157 22.206185237570978 76-77 21.49727004660896 27.89434034053077 28.485988775801392 22.12240083705888 78-79 21.416279712274896 27.74750303106874 28.609749804588347 22.226467452068015 80-81 21.26915316865974 28.439200091616378 28.258174041442714 22.033472698281166 82-83 21.319738230594385 28.103526550065094 28.163690740080426 22.4130444792601 84-85 21.315684018764607 27.771335758509398 28.065622082776926 22.847358139949073 86-87 21.487091586221386 27.800451046670315 28.238824873480876 22.473632493627424 88-89 21.649391923193022 27.486541002408405 28.149831769667383 22.714235304731194 90-91 21.38501901279596 27.43185686059008 28.511392455162376 22.671731671451578 92-93 21.31913988267529 27.630186240512995 28.274014437797845 22.776659439013873 94-95 21.155575856195345 27.416521548811936 28.39140902299999 23.03649357199273 96-97 21.18349276923604 27.503587783644274 28.132867904848553 23.180051542271134 98-99 21.05611473425399 27.654042593538204 28.071341834677305 23.218500837530506 100-101 21.008881716974564 27.567896054287903 28.351167081827093 23.072055146910436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1435.0 1 1055.5 2 771.5 3 840.0 4 766.5 5 864.5 6 1415.5 7 2822.5 8 4516.0 9 5090.0 10 4259.0 11 3455.5 12 3405.5 13 3445.5 14 3564.5 15 3620.5 16 3553.0 17 3550.0 18 3571.0 19 3710.5 20 3898.5 21 4224.5 22 4922.0 23 5746.5 24 6450.0 25 7264.0 26 8594.0 27 10005.0 28 10882.0 29 12566.5 30 15198.5 31 17398.5 32 19805.0 33 22570.0 34 25504.5 35 28139.5 36 32065.0 37 36166.5 38 37390.0 39 37020.0 40 37382.5 41 39273.5 42 40680.5 43 42716.0 44 45432.5 45 47042.5 46 46445.0 47 47406.5 48 56708.5 49 55652.0 50 45116.0 51 41169.5 52 38820.0 53 39013.5 54 39023.0 55 36414.0 56 33483.0 57 29777.0 58 26884.0 59 24992.0 60 22143.5 61 18804.0 62 15380.5 63 11998.0 64 8937.5 65 6653.0 66 4802.5 67 3886.5 68 2833.0 69 1629.0 70 1080.0 71 699.5 72 460.5 73 355.0 74 275.5 75 182.5 76 125.0 77 61.0 78 33.5 79 27.5 80 21.0 81 15.5 82 13.0 83 11.0 84 7.5 85 7.0 86 6.0 87 5.0 88 4.0 89 1.5 90 1.5 91 3.0 92 3.5 93 2.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06008938867033592 2 0.09766427221862192 3 0.06883657816032153 4 0.13029509214213347 5 0.10587902408747797 6 0.10268439836070063 7 0.11348527581790024 8 0.09682758452827549 9 0.11797296433884939 10-11 0.10846514967582156 12-13 0.09173139586889256 14-15 0.09648530320040648 16-17 0.1092638061075159 18-19 0.09036227055741655 20-21 0.07419898563026923 22-23 0.07537795464848468 24-25 0.06290370181059217 26-27 0.055982012735907895 28-29 0.06822807802188775 30-31 0.05001110512752641 32-33 0.05077173030056864 34-35 0.046398135555575835 36-37 0.052140855612044644 38-39 0.0503153551967433 40-41 0.06651667138254273 42-43 0.06381645201824283 44-45 0.04970685505830952 46-47 0.03749882103098178 48-49 0.04552341660657728 50-51 0.02886572531695251 52-53 0.04183438451732248 54-55 0.04731088576322651 56-57 0.04970685505830952 58-59 0.05837798203099092 60-61 0.05582988770129945 62-63 0.06176276405102882 64-65 0.042062572069235145 66-67 0.06480526474319773 68-69 0.05278738700913054 70-71 0.02525275574500193 72-73 0.020498848413488015 74-75 0.029284069162125732 76-77 0.04434444758836183 78-79 0.06199095160294149 80-81 0.04050329046449858 82-83 0.061116232653942934 84-85 0.06476723348454562 86-87 0.06655470264119485 88-89 0.08986786419493911 90-91 0.06510951481241462 92-93 0.07632873611478747 94-95 0.08522805063938152 96-97 0.09302445866306434 98-99 0.12333537180879708 100-101 0.11873358951189161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1314708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.44039851490509 #Duplication Level Percentage of deduplicated Percentage of total 1 70.40488132169943 31.288209833309207 2 14.735972045267767 13.097449403924013 3 5.786989805358397 7.715283994554606 4 2.837508664647452 5.0440006338571575 5 1.594650998048772 3.543346292273928 6 0.9828557648343728 2.620710112314682 7 0.6636925251111915 2.06463322151135 8 0.48147056676899086 1.711739508832894 9 0.35952377189914453 1.4379641728901837 >10 1.893057785965096 15.492200352931606 >50 0.1685263324293028 5.135111412123149 >100 0.08572681592898714 6.749016211221227 >500 0.003257614626047008 1.020321551636717 >1k 0.0013716272109671613 1.1360611108134906 >5k 3.4290680274179033E-4 1.0546091103131805 >10k+ 1.7145340137089517E-4 0.8893430774926266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11672 0.8878017019748872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8231 0.626070579931057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5610 0.42671072207668925 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2459 0.1870377300510836 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2140 0.16277378703103654 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2073 0.15767759837165363 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.1385098440109895 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1747 0.13288121773047704 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1735 0.13196846752282637 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1532 0.11652777651006915 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 0.10671571177782442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.803125865211134E-5 0.0 22-23 0.0 0.0 0.0 3.803125865211134E-4 0.0 24-25 0.0 0.0 0.0 0.0010648752422591176 0.0 26-27 0.0 0.0 0.0 0.001749437897997122 0.0 28-29 0.0 0.0 0.0 0.0037650946065590227 0.0 30-31 0.0 0.0 0.0 0.007948533058291272 0.0 32-33 0.0 0.0 0.0 0.013348971786891081 0.0 34-35 0.0 0.0 0.0 0.018635316739534558 0.0 36-37 0.0 0.0 0.0 0.025328818262306152 0.0 38-39 0.0 0.0 0.0 0.030463038180341184 0.0 40-41 0.0 0.0 0.0 0.03445632033881288 0.0 42-43 0.0 0.0 0.0 0.040845571792367585 0.0 44-45 0.0 0.0 0.0 0.047120729469965955 0.0 46-47 0.0 0.0 0.0 0.055297450080169896 0.0 48-49 0.0 0.0 0.0 0.0663265150892822 0.0 50-51 0.0 0.0 0.0 0.07503567332061567 0.0 52-53 0.0 0.0 0.0 0.09226383349002212 0.0 54-55 0.0 0.0 0.0 0.12630180998366178 0.0 56-57 0.0 0.0 0.0 0.1921339187104665 0.0 58-59 0.0 0.0 0.0 0.26789218594547226 0.0 60-61 0.0 0.0 0.0 0.33699498291635865 0.0 62-63 0.0 0.0 0.0 0.4239724714537373 0.0 64-65 0.0 0.0 0.0 0.5327799024574278 0.0 66-67 0.0 0.0 0.0 0.6942225954356405 0.0 68-69 0.0 0.0 0.0 0.883618263523155 0.0 70-71 0.0 0.0 0.0 1.0402309866525494 0.0 72-73 0.0 0.0 0.0 1.2093559938784886 0.0 74-75 0.0 0.0 0.0 1.3917158791153625 0.0 76-77 0.0 0.0 0.0 1.6004314265981496 0.0 78-79 0.0 0.0 0.0 1.774462466190211 0.0 80-81 0.0 0.0 0.0 1.9365516905655096 0.0 82-83 0.0 0.0 0.0 2.0848355680500914 0.0 84-85 0.0 0.0 0.0 2.375546509186831 0.0 86-87 0.0 0.0 0.0 2.771756161824527 0.0 88-89 0.0 0.0 0.0 3.2242140460086954 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 50 0.0016119326 38.01902 6 GTATCAA 13365 0.0 28.806677 1 GTACATG 8555 0.0 26.001772 1 GGTATCA 9375 0.0 24.944277 1 GAGTACT 7625 0.0 24.64534 12-13 TACATGG 8885 0.0 24.608067 2 ATCAACG 16015 0.0 23.919498 3 TATCAAC 16010 0.0 23.899105 2 TCAACGC 16020 0.0 23.853607 4 ACATGGG 8745 0.0 23.804457 3 CAACGCA 16235 0.0 23.47648 5 AACGCAG 16465 0.0 23.292856 6 AGTACTT 7915 0.0 22.811869 12-13 GTACTTT 8185 0.0 22.756414 14-15 TGGACCG 190 3.2718526E-7 22.511261 5 GTCGGGA 445 0.0 22.430367 2 CATGGGG 3110 0.0 21.54861 4 ACGCAGA 17740 0.0 21.510786 7 CGCAGAG 17790 0.0 21.396088 8 CATGGGA 5600 0.0 21.388142 4 >>END_MODULE