FastQCFastQC Report
Thu 26 May 2016
SRR937079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314708
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128470.9771751598073488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100240.7624506734575283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.5406523729984148No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.19882742023323807No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25920.1971540448525452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25350.19281848136620452No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23790.18095272866674578No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.16208922437529855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.15242928467766226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17650.13425034304195305No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14040.10679177429512865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGC958.7033186E-734.9989052
GTACGCT753.079939E-431.6644743
GTATCAA151700.028.284431
GGTATCA106400.026.3931431
GAGTACT98400.023.4833212-13
TATCAAC183050.023.457342
TCAACGC183100.023.398164
ATCAACG183050.023.3786073
GTACATG83900.023.2768971
CAACGCA184850.023.125265
AACGCAG186650.022.9031166
AGTACTT102250.022.08813312-13
GTACTTT105000.022.03026414-15
GTGTACG1754.0377345E-621.721871
TACATGG89750.021.6455632
ACATGGG86700.021.2009533
ACGCAGA204500.020.8110857
CGCAGAG205750.020.5461398
GCAGAGT207900.019.8995729
ACGTCGT600.004765961319.79104822-23
Produced by FastQC (version 0.11.5)