##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1314708 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.115126704941325 31.0 31.0 34.0 31.0 34.0 2 32.086269346501275 33.0 31.0 34.0 30.0 34.0 3 32.25506424240211 33.0 31.0 34.0 30.0 34.0 4 36.01885057366351 37.0 35.0 37.0 35.0 37.0 5 35.955026515393534 37.0 35.0 37.0 35.0 37.0 6 36.0128492410482 37.0 35.0 37.0 35.0 37.0 7 35.943744162201796 37.0 35.0 37.0 35.0 37.0 8 35.92822664804656 37.0 35.0 37.0 35.0 37.0 9 37.59445595523873 39.0 37.0 39.0 35.0 39.0 10-11 37.52680899484905 39.0 37.0 39.0 35.0 39.0 12-13 37.42775201793859 39.0 37.0 39.0 34.0 39.0 14-15 38.56667982548216 40.0 38.0 41.0 34.0 41.0 16-17 38.50866846478458 40.0 38.0 41.0 34.0 41.0 18-19 38.615315339984235 40.0 38.0 41.0 34.0 41.0 20-21 38.70593926560119 40.0 38.0 41.0 34.0 41.0 22-23 38.62804097944182 40.0 38.0 41.0 34.0 41.0 24-25 38.51626787088844 40.0 38.0 41.0 34.0 41.0 26-27 38.33218174682135 40.0 38.0 41.0 34.0 41.0 28-29 38.13572823775317 40.0 37.5 41.0 33.5 41.0 30-31 37.927111191230296 40.0 37.0 41.0 33.0 41.0 32-33 37.820807357983675 40.0 37.0 41.0 33.0 41.0 34-35 37.69915258749471 40.0 36.0 41.0 33.0 41.0 36-37 37.500086330957146 40.0 36.0 41.0 33.0 41.0 38-39 37.297529565500476 40.0 36.0 41.0 33.0 41.0 40-41 37.065303854544126 40.0 35.5 41.0 32.0 41.0 42-43 36.7762122844008 39.0 35.0 41.0 31.0 41.0 44-45 36.326184217331914 39.0 35.0 41.0 30.0 41.0 46-47 35.87076864216236 39.0 35.0 41.0 29.5 41.0 48-49 35.410496475262946 39.0 35.0 40.5 27.0 41.0 50-51 35.09617953188085 39.0 35.0 40.0 26.0 41.0 52-53 34.82132306185099 38.5 34.5 40.0 25.0 41.0 54-55 34.56181828968866 38.0 34.0 40.0 24.0 41.0 56-57 34.28245701707147 38.0 34.0 40.0 23.5 41.0 58-59 33.949776300136605 38.0 34.0 40.0 23.0 41.0 60-61 33.61747171234981 37.0 33.0 40.0 22.0 41.0 62-63 33.2380395494665 36.5 33.0 39.5 21.5 41.0 64-65 32.82324326010034 36.0 32.5 39.0 20.0 40.5 66-67 32.438491284756765 35.5 32.0 39.0 18.5 40.0 68-69 32.017921470014635 35.0 31.5 38.5 17.0 40.0 70-71 31.54719679198727 35.0 31.0 37.5 13.0 40.0 72-73 31.222343668708184 35.0 31.0 37.0 9.5 39.0 74-75 30.801455532331133 35.0 31.0 36.5 7.5 39.0 76-77 29.404626730802583 33.5 28.5 35.0 7.0 37.5 78-79 30.106144101960282 34.0 30.0 36.0 2.0 37.5 80-81 29.956561837305316 34.0 30.0 35.0 2.0 37.0 82-83 29.64843410095626 34.0 30.0 35.0 2.0 37.0 84-85 29.282857866537665 34.0 29.5 35.0 2.0 36.0 86-87 28.91793082570426 34.0 29.0 35.0 2.0 36.0 88-89 28.562349966684618 34.0 29.0 35.0 2.0 35.5 90-91 28.15676294660107 34.0 29.0 35.0 2.0 35.0 92-93 27.733251033689612 33.5 27.0 35.0 2.0 35.0 94-95 27.281681940020142 33.0 27.0 35.0 2.0 35.0 96-97 26.68335440265063 33.0 25.5 35.0 2.0 35.0 98-99 25.9977907641849 33.0 24.5 35.0 2.0 35.0 100-101 23.167447448406797 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3558822775139703 1101 2 0.37019335570174405 1101 3 0.3019329648110656 1101 4 0.22226101143715482 1101 5 0.2654963840569522 1101 6 0.16908841696560017 1101 7 0.3114647824805843 1101 8 0.35235450638989363 1101 9 0.3023628752343086 1101 10-11 0.41838050132717086 1101 12-13 0.4123024792739258 1101 14-15 0.6543215142304106 1101 16-17 0.6168753545163526 1101 18-19 0.5564986529041533 1101 20-21 0.5513303791718798 1101 22-23 0.39205441259492346 1101 24-25 0.4235686014435842 1101 26-27 0.47554167687254534 1101 28-29 0.5031360692838334 1101 30-31 0.5484861351939259 1101 32-33 0.6330027222248304 1101 34-35 0.6572717832405957 1101 36-37 0.7638946762627299 1101 38-39 0.6849158916023512 1101 40-41 0.593062171386741 1101 42-43 0.8220244627679492 1101 44-45 0.8982248630104266 1101 46-47 0.9833287721252901 1101 48-49 0.796802253728508 1101 50-51 0.8479870926609792 1101 52-53 0.7455830745739931 1101 54-55 0.9469003448289044 1101 56-57 1.011831378261867 1101 58-59 1.007111310088277 1101 60-61 0.9538413813981634 1101 62-63 1.0270426532764176 1101 64-65 1.1495459590093091 1101 66-67 1.1516074355137036 1101 68-69 1.1066422167276357 1101 70-71 1.0581189123737218 1101 72-73 1.2554371932563448 1101 74-75 0.9696883361337427 1101 76-77 0.7855105740581045 1101 78-79 0.9266343158929082 1101 80-81 1.1422910115120466 1101 82-83 1.255020494413234 1101 84-85 1.2585079665629841 1101 86-87 1.0426085217518875 1101 88-89 1.144408186739696 1101 90-91 1.1077303542123005 1101 92-93 1.231303985811092 1101 94-95 1.3675675166768997 1101 96-97 1.4552046229335183 1101 98-99 1.3710562749003152 1101 100-101 1.6307630018248709 1102 1 0.23681077920290505 1102 2 0.07131409694407864 1102 3 0.1486211335944816 1102 4 0.07809561770629614 1102 5 0.130451345750771 1102 6 0.1673994804837875 1102 7 0.18351012786612841 1102 8 0.16896956666406027 1102 9 0.20947432142062183 1102 10-11 0.23638184775205673 1102 12-13 0.28556211684154675 1102 14-15 0.2920891752305437 1102 16-17 0.35205385423682856 1102 18-19 0.2520791706872032 1102 20-21 0.16353171496709962 1102 22-23 0.22356971144612459 1102 24-25 0.34130455059439413 1102 26-27 0.22902904528115897 1102 28-29 0.1537425018090488 1102 30-31 0.18817501065355913 1102 32-33 0.4260890567996114 1102 34-35 0.37639185323894253 1102 36-37 0.30518085651043947 1102 38-39 0.22275604104763858 1102 40-41 0.2457554148534271 1102 42-43 0.15229397411807355 1102 44-45 0.387809508648715 1102 46-47 0.30746063318368755 1102 48-49 0.21575643197626704 1102 50-51 0.37416708447219804 1102 52-53 0.4377517635025967 1102 54-55 0.23588835101773498 1102 56-57 0.29403115897570586 1102 58-59 0.16253800276482622 1102 60-61 0.22680097684079215 1102 62-63 0.3018297153930618 1102 64-65 0.26393917216175566 1102 66-67 0.34246463967424035 1102 68-69 0.15183553893262314 1102 70-71 0.2037055323748227 1102 72-73 0.36956491071184416 1102 74-75 -0.0054906458588135365 1102 76-77 0.039801073101433104 1102 78-79 0.44428571979675624 1102 80-81 0.389337587183487 1102 82-83 0.4844693066460124 1102 84-85 0.4798213902616375 1102 86-87 0.2479818692318716 1102 88-89 0.5392079158799525 1102 90-91 0.6332543575822989 1102 92-93 0.3566049137419256 1102 94-95 0.5821094467887882 1102 96-97 0.5549165883988998 1102 98-99 0.5794125735704263 1102 100-101 0.8769469010241018 1103 1 0.045427646106290354 1103 2 -0.15211704432414308 1103 3 0.09992287492431018 1103 4 0.04021378979638257 1103 5 0.1453738452238298 1103 6 0.16846146469183765 1103 7 0.246408371944149 1103 8 0.28146794531412667 1103 9 0.1690763610788366 1103 10-11 0.2764109509834114 1103 12-13 0.31884184429262064 1103 14-15 0.3464636786921247 1103 16-17 0.38302771807586566 1103 18-19 0.3017542926937793 1103 20-21 0.2974253260376756 1103 22-23 0.3783914034869227 1103 24-25 0.4477618807174508 1103 26-27 0.5175345862445724 1103 28-29 0.46261321131178335 1103 30-31 0.431144938665625 1103 32-33 0.42089415027043486 1103 34-35 0.2864459455261752 1103 36-37 0.37307032503193227 1103 38-39 0.4485665133468757 1103 40-41 0.529892937755001 1103 42-43 0.5389448934234835 1103 44-45 0.6418299498180318 1103 46-47 0.5187098630424671 1103 48-49 0.47903427460804693 1103 50-51 0.3267010517533535 1103 52-53 0.6554139390299341 1103 54-55 0.5859758025799096 1103 56-57 0.5278130995694426 1103 58-59 0.5530305080747482 1103 60-61 0.44241113135544197 1103 62-63 0.3887953792547947 1103 64-65 0.5247824565303176 1103 66-67 0.39860286786302623 1103 68-69 0.43518164039669927 1103 70-71 0.36642319860372297 1103 72-73 0.38945178102006395 1103 74-75 0.34237960966594017 1103 76-77 0.4812769271475119 1103 78-79 0.4470911618578164 1103 80-81 0.5651066137249856 1103 82-83 0.5958428737780075 1103 84-85 0.6063104988434418 1103 86-87 0.4317421242702153 1103 88-89 0.6880559467504561 1103 90-91 0.7329572452065349 1103 92-93 0.778578968089576 1103 94-95 0.8458782544480421 1103 96-97 1.0866580600982338 1103 98-99 0.9539504888386752 1103 100-101 1.081388487839284 1104 1 0.2643706102486618 1104 2 0.16928328520026525 1104 3 0.24652393511473747 1104 4 0.19215736566905406 1104 5 0.20045408420362776 1104 6 0.2772695284482012 1104 7 0.3072446307124608 1104 8 0.18538472441554177 1104 9 0.2479682553326228 1104 10-11 0.28393342194753046 1104 12-13 0.3697337555981193 1104 14-15 0.3478773724987363 1104 16-17 0.4611085901006078 1104 18-19 0.4356187869718653 1104 20-21 0.3156221710439766 1104 22-23 0.28783933287576957 1104 24-25 0.29099533362594343 1104 26-27 0.2787221103567603 1104 28-29 0.32682880041658535 1104 30-31 0.4129464950609787 1104 32-33 0.37559520172185046 1104 34-35 0.35778126538439636 1104 36-37 0.32058620977086605 1104 38-39 0.30063701792845876 1104 40-41 0.5015476575633357 1104 42-43 0.541664109215084 1104 44-45 0.6849809150517174 1104 46-47 0.7934359165720721 1104 48-49 0.8982608300975201 1104 50-51 0.6797109992980097 1104 52-53 0.7212895126603129 1104 54-55 0.710534323002534 1104 56-57 0.6563984892331263 1104 58-59 0.6362141060293922 1104 60-61 0.6648694132671977 1104 62-63 0.7374475477001639 1104 64-65 0.7041750317870026 1104 66-67 0.7053957385406804 1104 68-69 0.5868086937962786 1104 70-71 0.7026340089652052 1104 72-73 0.7228927533088942 1104 74-75 0.7130892381303653 1104 76-77 0.590411104967064 1104 78-79 0.6905187911790911 1104 80-81 0.8126469037320412 1104 82-83 0.6985613435570386 1104 84-85 0.8180778878634172 1104 86-87 0.7001590429825555 1104 88-89 0.9534238055451958 1104 90-91 0.8496730421291261 1104 92-93 1.0335644634738905 1104 94-95 1.1145056552763322 1104 96-97 1.1006399341244233 1104 98-99 1.160335930083793 1104 100-101 0.8892092572553274 1105 1 0.27577219199608294 1105 2 0.11137803239599009 1105 3 0.15051497424030913 1105 4 0.1183518157302288 1105 5 0.12712205319013492 1105 6 0.113848379916341 1105 7 0.21510093228403804 1105 8 0.30703853352972743 1105 9 0.3052960321012961 1105 10-11 0.3716532312604883 1105 12-13 0.38263271956841294 1105 14-15 0.5065447680075579 1105 16-17 0.4209023209156584 1105 18-19 0.3284481453737129 1105 20-21 0.295416507323516 1105 22-23 0.32397585255164785 1105 24-25 0.3524685429647221 1105 26-27 0.37047482723586 1105 28-29 0.37877851030770415 1105 30-31 0.37663062081023213 1105 32-33 0.5436915642511408 1105 34-35 0.5883404289751866 1105 36-37 0.5491650475303658 1105 38-39 0.6321976540545862 1105 40-41 0.35828876209049554 1105 42-43 0.48874451375674965 1105 44-45 0.5817989135689032 1105 46-47 0.49842163103730286 1105 48-49 0.504758035250596 1105 50-51 0.5275594869505298 1105 52-53 0.46648035392465914 1105 54-55 0.38258876682628085 1105 56-57 0.45156671125809567 1105 58-59 0.13766036648650726 1105 60-61 0.1566148188769958 1105 62-63 0.22432753207232992 1105 64-65 0.33349939053727695 1105 66-67 0.3094079689233453 1105 68-69 0.4286605126458767 1105 70-71 0.4309615218427467 1105 72-73 0.40145279882154483 1105 74-75 0.35271163391907834 1105 76-77 0.45931719057060505 1105 78-79 0.36209971607750546 1105 80-81 0.6644460471711469 1105 82-83 0.6190330118173328 1105 84-85 0.5200811185305376 1105 86-87 0.5653001104964162 1105 88-89 0.5191452621785153 1105 90-91 0.6806584869913053 1105 92-93 0.6453080755379403 1105 94-95 0.6306592764680445 1105 96-97 0.7447479078632604 1105 98-99 0.9608256708878748 1105 100-101 1.069749057406348 1106 1 0.32985730858473516 1106 2 0.28815677399845896 1106 3 0.19009286753333043 1106 4 0.21693747261762297 1106 5 0.22604994419261004 1106 6 0.2904566781504627 1106 7 0.3521522215904369 1106 8 0.3453559543240843 1106 9 0.4422435732704386 1106 10-11 0.51294000458563 1106 12-13 0.4846198332413394 1106 14-15 0.6036654877231982 1106 16-17 0.5029024233164208 1106 18-19 0.4316024255906896 1106 20-21 0.4246294227539593 1106 22-23 0.45758930585555646 1106 24-25 0.5765365613214044 1106 26-27 0.5773179600523335 1106 28-29 0.5772746339000321 1106 30-31 0.6928913223632236 1106 32-33 0.6465127051572779 1106 34-35 0.6058859905759775 1106 36-37 0.5288000966153632 1106 38-39 0.8349565460783737 1106 40-41 0.9231768218925396 1106 42-43 0.8575226571232051 1106 44-45 1.0181497682110248 1106 46-47 0.7524321028622651 1106 48-49 0.9330737987695201 1106 50-51 0.9534476114267392 1106 52-53 0.8960916217807906 1106 54-55 0.8922484594623228 1106 56-57 0.8417802976829876 1106 58-59 0.7736337530682391 1106 60-61 0.9412848863267556 1106 62-63 0.8694644391921429 1106 64-65 1.0387637327067196 1106 66-67 0.9892391963042328 1106 68-69 1.1315046263538306 1106 70-71 0.9399370830932199 1106 72-73 1.260804682860769 1106 74-75 1.1265673054390035 1106 76-77 0.8775831954049984 1106 78-79 0.8452847704172761 1106 80-81 0.9122144516864843 1106 82-83 1.116401244946136 1106 84-85 1.0124098195769093 1106 86-87 0.844004996898942 1106 88-89 1.1980821671425304 1106 90-91 1.3801125966301768 1106 92-93 1.412795699867285 1106 94-95 1.3237668969708345 1106 96-97 1.6229354683268618 1106 98-99 1.6750992587679328 1106 100-101 1.640454760320189 1107 1 0.14224409586391573 1107 2 0.11712316609254003 1107 3 0.0514263264277659 1107 4 0.111697420103539 1107 5 0.05500387250091876 1107 6 0.08841732582416739 1107 7 0.19353808377974246 1107 8 0.11104924548366313 1107 9 0.09219122537017199 1107 10-11 0.1665076940213268 1107 12-13 0.18301560552519902 1107 14-15 0.16216842086745942 1107 16-17 0.18579878555281226 1107 18-19 0.22902782290848478 1107 20-21 0.29349377798848053 1107 22-23 0.22423399932951327 1107 24-25 0.23150781152220645 1107 26-27 0.2207075835352157 1107 28-29 0.18446665375346072 1107 30-31 0.24866260912446592 1107 32-33 0.15194414602630957 1107 34-35 0.20445237823849283 1107 36-37 0.22580961894769302 1107 38-39 0.24849803386663183 1107 40-41 0.31843185864686063 1107 42-43 0.1329252374038319 1107 44-45 0.2600379209671786 1107 46-47 0.050439835948914435 1107 48-49 0.075243356111244 1107 50-51 0.10086788474371389 1107 52-53 0.2903758681095141 1107 54-55 0.24583983009099342 1107 56-57 0.17560202677013592 1107 58-59 0.11385909831510332 1107 60-61 0.17558605570683739 1107 62-63 -0.0028579241632158414 1107 64-65 0.00935335286592931 1107 66-67 0.03460460680594224 1107 68-69 0.11579540924576293 1107 70-71 0.05276481141592626 1107 72-73 -0.052188675326274137 1107 74-75 -0.0973951536382387 1107 76-77 0.017899110828519582 1107 78-79 0.0877144054222363 1107 80-81 -0.03451325060076016 1107 82-83 -0.14952816571068084 1107 84-85 0.01364927382927661 1107 86-87 0.1362302258171404 1107 88-89 -0.07964490918569211 1107 90-91 0.1309165108128596 1107 92-93 0.172856744720292 1107 94-95 0.04054121381688347 1107 96-97 0.2756293078930163 1107 98-99 0.19185474144881098 1107 100-101 0.11092528208196129 1108 1 0.055153475832121046 1108 2 -0.035887718094812726 1108 3 0.05138474638618362 1108 4 0.13071662029921072 1108 5 0.06815661800660422 1108 6 0.0992318054621748 1108 7 0.08188955742533466 1108 8 0.10496999881618052 1108 9 0.10730923931171787 1108 10-11 0.1210490906215469 1108 12-13 0.19942015487092846 1108 14-15 0.1295794518078992 1108 16-17 0.22663466668281274 1108 18-19 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-0.365416710087743 2316 88-89 -0.7389106558178966 2316 90-91 -0.7151760098195012 2316 92-93 -1.1278019716611674 2316 94-95 -0.862208395104691 2316 96-97 -0.8616125484568364 2316 98-99 -0.9660055580320304 2316 100-101 -0.8084081973622119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 10.0 5 33.0 6 98.0 7 197.0 8 499.0 9 907.0 10 1609.0 11 2649.0 12 4248.0 13 5836.0 14 7412.0 15 10297.0 16 14203.0 17 20040.0 18 16984.0 19 9384.0 20 7896.0 21 7686.0 22 7915.0 23 8056.0 24 9643.0 25 11330.0 26 13718.0 27 16511.0 28 20527.0 29 25651.0 30 31631.0 31 39417.0 32 49831.0 33 65606.0 34 89908.0 35 129594.0 36 191096.0 37 276761.0 38 201773.0 39 15748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.883911861993 19.736641906634087 15.475197872321003 20.904248359051916 2 15.54768033395402 22.000544622537028 39.45447990069006 22.997295142818892 3 18.666927316679114 27.021394092169697 31.129750460574655 23.18192813057653 4 12.671102632675849 17.870736315592513 37.62105349628967 31.837107555441968 5 12.955641118867927 38.55445330672115 33.41505119853714 15.074854375873784 6 29.650529966798633 37.66350626186102 17.494951338893515 15.191012432446824 7 27.041766720645743 34.943375215922636 21.018903831804348 16.995954231627273 8 26.815612288051792 33.41707816488528 20.048938623633536 19.718370923429383 9 27.6479644149119 16.317539712240283 20.81237810981602 35.22211776303179 10-11 24.607061035606385 27.26236548343815 26.92164343717388 21.208930043781585 12-13 26.508049395487166 25.19272530891195 27.889738684637884 20.409486610962997 14-15 23.37937397505758 25.723506664597767 27.659221667472927 23.237897692871726 16-17 20.981236898231394 29.232194525324257 27.92414741524354 21.862421161200814 18-19 20.743845781724914 26.67535300614281 32.109943805012215 20.47085740712006 20-21 22.01907403167494 25.652883962250517 32.08595788895591 20.24208411711863 22-23 21.713642877353756 25.636034769697908 32.06392598204316 20.586396370905174 24-25 21.53293513378858 25.93305911951121 32.2211129430065 20.31289280369371 26-27 21.281567399692854 26.046275653444507 32.311226930820794 20.36093001604184 28-29 20.871291225665463 25.795342479611794 32.676273390384424 20.657092904338313 30-31 21.017643237190168 26.06888713409986 32.32618079297192 20.587288835738054 32-33 20.95447196708287 26.300044382900833 32.08317058969168 20.662313060324617 34-35 21.381479488315573 25.93590443283619 31.83320624259562 20.849409836252615 36-37 21.58593893810291 25.955033445757326 31.588517539816195 20.87051007632357 38-39 21.396043143578666 26.048962485167493 31.84600054766179 20.708993823592053 40-41 21.155050779926267 26.25803116966691 31.892334309643637 20.694583740763182 42-43 21.162284955376602 26.634993294890197 31.046598041027817 21.156123708705383 44-45 21.354252237779683 26.727091281938943 30.616659668851202 21.301996811430172 46-47 21.763510134383505 26.86601392324909 30.003023486212715 21.367452456154695 48-49 22.0036685077662 27.14889707070834 29.4397177766698 21.407716644855665 50-51 22.026424178808174 27.370018369139842 29.191187376635824 21.412370075416156 52-53 21.942752349125254 27.687712288396177 28.981666041044612 21.387869321433964 54-55 21.590845991241835 27.617199593202496 28.934004939683977 21.857949475871692 56-57 21.862914337719495 27.92390071221382 28.55347232284694 21.659712627219747 58-59 21.99136069443039 27.339659797868542 28.826893660782133 21.84208584691893 60-61 21.96284145856327 27.36848430543755 29.325027706130186 21.343646529868995 62-63 21.98868493992582 27.349761315820697 29.36557014941721 21.29598359483627 64-65 22.005291675366326 27.35131377231344 29.250330777249523 21.393063775070708 66-67 21.737352885471818 27.78969002218745 28.935040278936675 21.537916813404053 68-69 21.628879427553276 27.71818065995668 29.113776258217133 21.539163654272908 70-71 21.89989389254624 27.68446153319566 28.75557634602439 21.660068228233712 72-73 21.855866689637573 27.67115702249894 28.578165544933416 21.894810742930076 74-75 21.74191335196372 28.282807154190543 28.279498409520663 21.69578108432507 76-77 21.90666964327481 27.888701223971633 28.469482096804928 21.73514703594863 78-79 21.677494828685838 27.85127760648876 28.593079281313788 21.878148283511617 80-81 21.567986101021656 28.574487555044048 28.137900502727387 21.71962584120691 82-83 21.573807251853964 28.239969361470372 28.058557015010066 22.127666371665597 84-85 21.575665743992538 28.025713170811855 27.914657587492403 22.4839634977032 86-87 21.69764929106781 27.961604593027168 28.100534841523295 22.24021127438173 88-89 21.81320451253124 27.739676403909808 27.90636912225616 22.540749961302797 90-91 21.49105692912098 27.80627593262367 28.150041264009793 22.552625874245553 92-93 21.52915324163236 27.8289551748373 28.10753414446402 22.53435743906632 94-95 21.455057564288236 27.70452512504716 27.976867796857697 22.86354951380691 96-97 21.54712522063295 27.70377813367248 27.771475035626448 22.977621610068127 98-99 21.484528021164294 27.95019575299296 27.51586523431409 23.049410991528653 100-101 21.128876941893793 28.209106896195014 27.665407059883044 22.99660910202815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2431.0 1 1671.5 2 990.5 3 1077.5 4 968.5 5 958.5 6 1581.5 7 3089.5 8 4797.5 9 5426.0 10 4685.5 11 3817.5 12 3646.0 13 3759.5 14 3922.0 15 3967.5 16 3946.5 17 4017.5 18 4111.5 19 4282.5 20 4438.0 21 4682.5 22 5187.0 23 5944.0 24 6726.5 25 7567.0 26 8792.0 27 10008.0 28 10957.5 29 12550.5 30 14904.0 31 17082.5 32 19566.5 33 22392.0 34 25313.0 35 27697.5 36 31324.0 37 35152.0 38 36482.0 39 36780.0 40 37144.5 41 38707.0 42 40290.0 43 42267.0 44 44847.5 45 46524.5 46 45993.5 47 47091.0 48 56691.0 49 55380.0 50 44783.0 51 41227.0 52 38772.5 53 38579.0 54 38169.0 55 35524.5 56 32666.0 57 29357.0 58 27236.5 59 25675.5 60 22613.0 61 18952.5 62 15469.0 63 12150.0 64 8898.0 65 6721.0 66 5090.0 67 4063.0 68 3002.0 69 1799.0 70 1158.5 71 736.0 72 496.0 73 358.0 74 282.0 75 221.5 76 142.5 77 65.0 78 32.0 79 16.5 80 11.5 81 9.5 82 5.5 83 1.5 84 1.5 85 1.5 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01703800387614588 2 0.0027382506229520166 3 0.0034988757959942434 4 0.0 5 0.001673375380692899 6 2.2818755191266805E-4 7 7.606251730422268E-5 8 0.0 9 0.0 10-11 0.0 12-13 9.888127249548949E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0031185632094731302 22-23 0.0 24-25 0.0030805319508210188 26-27 0.0015973128633886763 28-29 0.0029284069162125734 30-31 0.015973128633886762 32-33 9.507814663027835E-4 34-35 0.002319906777778792 36-37 0.0031946257267773527 38-39 0.002129750484518235 40-41 0.004906032366122363 42-43 0.0029664381748646844 44-45 9.127502076506722E-4 46-47 3.803125865211134E-5 48-49 3.422813278690021E-4 50-51 2.2818755191266805E-4 52-53 0.0012550315355196744 54-55 0.0037270633479069117 56-57 3.422813278690021E-4 58-59 0.001445187828780231 60-61 0.0012930627941717856 62-63 0.0 64-65 1.1409377595633402E-4 66-67 7.606251730422268E-5 68-69 3.803125865211134E-5 70-71 2.2818755191266805E-4 72-73 1.1409377595633402E-4 74-75 7.606251730422268E-4 76-77 9.507814663027835E-4 78-79 2.662188105647794E-4 80-81 0.006008938867033592 82-83 0.001787469156649233 84-85 0.0038791883825153566 86-87 0.0014832190874323422 88-89 0.0010268439836070061 90-91 2.2818755191266805E-4 92-93 0.0 94-95 3.0425006921689073E-4 96-97 0.0018635316739534558 98-99 0.0031185632094731302 100-101 9.127502076506722E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1314708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.94219458290815 #Duplication Level Percentage of deduplicated Percentage of total 1 68.46151599345104 28.714262252382017 2 15.36610970571863 12.889767265191285 3 6.197502206536164 7.7981053042362705 4 3.0791045024058676 5.165776007240619 5 1.7892603510139926 3.7522752900855703 6 1.0637556965622723 2.676974904833509 7 0.7653363281723222 2.2469919638300384 8 0.5271774521313438 1.7688783421611651 9 0.39817177712966023 1.503017833841509 >10 2.0710322911507384 15.995285103764706 >50 0.17783674242662054 5.149856896421507 >100 0.09570833081838591 7.14614727459474 >500 0.005296830536856904 1.4756066712304414 >1k 0.0016438439597142118 1.420449532652624 >5k 1.8264932885713467E-4 0.5445235291821191 >10k+ 3.6529865771426934E-4 1.7520818283517507 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12847 0.9771751598073488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10024 0.7624506734575283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7108 0.5406523729984148 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2614 0.19882742023323807 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2592 0.1971540448525452 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2535 0.19281848136620452 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2379 0.18095272866674578 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2131 0.16208922437529855 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2004 0.15242928467766226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1765 0.13425034304195305 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1404 0.10679177429512865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.606251730422268E-5 0.0 22-23 0.0 0.0 0.0 4.563751038253361E-4 0.0 24-25 0.0 0.0 0.0 0.0011789690182154515 0.0 26-27 0.0 0.0 0.0 0.0018635316739534558 0.0 28-29 0.0 0.0 0.0 0.0038411571238632456 0.0 30-31 0.0 0.0 0.0 0.008024595575595494 0.0 32-33 0.0 0.0 0.0 0.013577159338803749 0.0 34-35 0.0 0.0 0.0 0.018825473032795116 0.0 36-37 0.0 0.0 0.0 0.02551897455556671 0.0 38-39 0.0 0.0 0.0 0.03069122573225385 0.0 40-41 0.0 0.0 0.0 0.03472253914937766 0.0 42-43 0.0 0.0 0.0 0.04103572808562814 0.0 44-45 0.0 0.0 0.0 0.04731088576322651 0.0 46-47 0.0 0.0 0.0 0.05567776266669101 0.0 48-49 0.0 0.0 0.0 0.0667448589344554 0.0 50-51 0.0 0.0 0.0 0.07522582961387624 0.0 52-53 0.0 0.0 0.0 0.0924920210419348 0.0 54-55 0.0 0.0 0.0 0.12660606005287867 0.0 56-57 0.0 0.0 0.0 0.19323682521137775 0.0 58-59 0.0 0.0 0.0 0.2692993425156004 0.0 60-61 0.0 0.0 0.0 0.3388204833316599 0.0 62-63 0.0 0.0 0.0 0.42595009690364705 0.0 64-65 0.0 0.0 0.0 0.5344913090967729 0.0 66-67 0.0 0.0 0.0 0.6963143146615065 0.0 68-69 0.0 0.0 0.0 0.8873072956124097 0.0 70-71 0.0 0.0 0.0 1.0438059249658478 0.0 72-73 0.0 0.0 0.0 1.2133873072956125 0.0 74-75 0.0 0.0 0.0 1.3961275051190074 0.0 76-77 0.0 0.0 0.0 1.606364302947879 0.0 78-79 0.0 0.0 0.0 1.7814602177821994 0.0 80-81 0.0 0.0 0.0 1.9441199110372798 0.0 82-83 0.0 0.0 0.0 2.093848976350642 0.0 84-85 0.0 0.0 0.0 2.383685198538383 0.0 86-87 0.0 0.0 0.0 2.780123038727991 0.0 88-89 0.0 0.0 0.0 3.2341782357755484 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACGC 95 8.7033186E-7 34.998905 2 GTACGCT 75 3.079939E-4 31.664474 3 GTATCAA 15170 0.0 28.28443 1 GGTATCA 10640 0.0 26.393143 1 GAGTACT 9840 0.0 23.48332 12-13 TATCAAC 18305 0.0 23.45734 2 TCAACGC 18310 0.0 23.39816 4 ATCAACG 18305 0.0 23.378607 3 GTACATG 8390 0.0 23.276897 1 CAACGCA 18485 0.0 23.12526 5 AACGCAG 18665 0.0 22.903116 6 AGTACTT 10225 0.0 22.088133 12-13 GTACTTT 10500 0.0 22.030264 14-15 GTGTACG 175 4.0377345E-6 21.72187 1 TACATGG 8975 0.0 21.645563 2 ACATGGG 8670 0.0 21.200953 3 ACGCAGA 20450 0.0 20.811085 7 CGCAGAG 20575 0.0 20.546139 8 GCAGAGT 20790 0.0 19.899572 9 ACGTCGT 60 0.0047659613 19.791048 22-23 >>END_MODULE