FastQCFastQC Report
Thu 26 May 2016
SRR937078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241642
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116980.9421395216978807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.6595298805936011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56550.45544528938293No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23490.18918496635906323No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18870.15197617348639947No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.15117078835928552No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18040.1452914769313538No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.13820408781275118No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16540.1332107000246448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.1121096096942597No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13810.11122368605443436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124900.030.4050541
GGTATCA84150.028.1278571
GTACATG77950.026.7676241
TACATGG80950.025.3059022
TCAACGC150400.024.9655254
TATCAAC150500.024.9489362
ATCAACG150600.024.932373
CAACGCA151700.024.7222425
ACATGGG80250.024.6382433
AACGCAG153500.024.42846
GAGTACT76150.024.27810912-13
AGTACTT81100.022.6204712-13
CATGGGA49050.022.577634
CATGGGG28450.022.5534484
GTACTTT82150.022.3589214-15
ACGCAGA166850.022.2753457
CGCAGAG166400.022.195468
GCAGAGT168150.021.6252449
TAACACG1100.002867214421.6042294
ATGGGAG17000.020.97055
Produced by FastQC (version 0.11.5)