FastQCFastQC Report
Thu 26 May 2016
SRR937078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241642
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121280.9767710821637798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93940.7565787884108301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67580.544279268903597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25780.20762828576997233No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24620.19828581829545072No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.19103735215142528No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22060.17766795904133398No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.16607041321089333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.14698278569829307No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17500.14094239724493854No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13480.10856591513495838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139500.027.1401441
GGTATCA99700.027.0633281
GTACATG77250.025.150861
GAGTACT87750.024.21727612-13
TACATGG83850.022.9947432
ACATGGG82650.022.754013
GTACTTT93800.022.68151314-15
TATCAAC168550.022.2870882
ATCAACG169200.022.2295363
TCAACGC169600.022.1762144
AGTACTT91050.021.98350512-13
CAACGCA171550.021.9518225
AACGCAG174050.021.6356416
CATGGGG28650.021.382244
ATGGGAG17500.020.6235625
ACAGCGC2108.632269E-720.3513778
TACTTTT100100.019.97307214-15
ACTTTTT108100.019.90114616-17
ACGCAGA188500.019.8763337
CGCAGAG188700.019.7797728
Produced by FastQC (version 0.11.5)