##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937078_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241642 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19155440940303 33.0 31.0 34.0 31.0 34.0 2 32.2441380043523 33.0 31.0 34.0 30.0 34.0 3 32.36868356579433 34.0 31.0 34.0 31.0 34.0 4 36.0676354375899 37.0 35.0 37.0 35.0 37.0 5 35.98814553631401 37.0 35.0 37.0 35.0 37.0 6 36.035341104762885 37.0 35.0 37.0 35.0 37.0 7 35.98579944943873 37.0 35.0 37.0 35.0 37.0 8 35.97593589778696 37.0 35.0 37.0 35.0 37.0 9 37.653098880353596 39.0 37.0 39.0 35.0 39.0 10-11 37.55682918264685 39.0 37.0 39.0 35.0 39.0 12-13 37.479167908302074 39.0 37.0 39.0 35.0 39.0 14-15 38.643851851016635 40.0 38.0 41.0 34.0 41.0 16-17 38.58841074963637 40.0 38.0 41.0 34.0 41.0 18-19 38.673492037157246 40.0 38.0 41.0 34.5 41.0 20-21 38.76745712532276 40.0 38.0 41.0 35.0 41.0 22-23 38.700002094001334 40.0 38.0 41.0 34.5 41.0 24-25 38.59974372645255 40.0 38.0 41.0 34.0 41.0 26-27 38.40574175164822 40.0 38.0 41.0 34.0 41.0 28-29 38.23329188284546 40.0 38.0 41.0 34.0 41.0 30-31 38.11387058427469 40.0 37.0 41.0 34.0 41.0 32-33 37.95147997570959 40.0 37.0 41.0 33.5 41.0 34-35 37.79427202043745 40.0 37.0 41.0 33.0 41.0 36-37 37.585258069556275 40.0 36.0 41.0 33.0 41.0 38-39 37.411144677773464 40.0 36.0 41.0 33.0 41.0 40-41 37.1320984631641 40.0 36.0 41.0 32.5 41.0 42-43 36.84242962142066 39.5 35.0 41.0 31.0 41.0 44-45 36.49029913614392 39.0 35.0 41.0 30.5 41.0 46-47 35.97177326475747 39.0 35.0 41.0 29.5 41.0 48-49 35.478107618782225 39.0 35.0 41.0 27.5 41.0 50-51 35.18579751651443 39.0 35.0 40.0 26.0 41.0 52-53 34.928584084623424 38.5 35.0 40.0 26.0 41.0 54-55 34.64876631106229 38.0 34.0 40.0 24.5 41.0 56-57 34.39255155672892 38.0 34.0 40.0 24.0 41.0 58-59 34.05203995998846 38.0 34.0 40.0 23.0 41.0 60-61 33.708553673281024 37.0 33.5 40.0 22.0 41.0 62-63 33.36011225457902 37.0 33.0 39.5 22.0 41.0 64-65 32.93702774229609 36.0 33.0 39.0 20.0 40.5 66-67 32.51384175148714 35.5 32.0 39.0 18.5 40.0 68-69 32.09135684843135 35.0 32.0 38.5 17.5 40.0 70-71 31.70694531918218 35.0 31.0 38.0 14.5 40.0 72-73 31.336611519262398 35.0 31.0 37.0 10.0 39.0 74-75 30.87541819622725 35.0 31.0 36.5 7.5 39.0 76-77 29.42200569890516 33.5 28.5 35.0 7.0 37.5 78-79 30.11048716135569 34.0 30.0 36.0 4.0 37.5 80-81 29.84581062818429 34.0 30.0 35.0 2.0 37.0 82-83 29.663477878486713 34.0 30.0 35.0 2.0 37.0 84-85 29.350286556028227 34.0 30.0 35.0 2.0 36.0 86-87 28.98608737462167 34.0 29.0 35.0 2.0 36.0 88-89 28.62285948767841 34.0 29.0 35.0 2.0 35.5 90-91 28.191394137762735 34.0 29.0 35.0 2.0 35.0 92-93 27.789956766926377 33.5 27.5 35.0 2.0 35.0 94-95 27.335670024048802 33.0 27.0 35.0 2.0 35.0 96-97 26.740936598472025 33.0 25.5 35.0 2.0 35.0 98-99 26.023117371996115 33.0 24.5 34.5 2.0 35.0 100-101 23.01135150067411 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28137054425363317 1101 2 0.15713127221768985 1101 3 0.1434502091728902 1101 4 0.08444105691275183 1101 5 0.1743855888893293 1101 6 0.1510413209474848 1101 7 0.0948730490847467 1101 8 0.2275997868682964 1101 9 0.308994211845139 1101 10-11 0.2453945104421038 1101 12-13 0.16236367538507324 1101 14-15 0.20966351359938074 1101 16-17 0.23646061750349645 1101 18-19 0.37179739227391195 1101 20-21 0.28773456366998573 1101 22-23 0.3157301159428698 1101 24-25 0.30535625315905435 1101 26-27 0.2260515762014137 1101 28-29 0.20816450685023824 1101 30-31 0.19750299920622894 1101 32-33 0.09960769121843072 1101 34-35 0.22489002542092607 1101 36-37 0.18458482442179047 1101 38-39 0.09286383858198377 1101 40-41 0.3092019732385012 1101 42-43 0.13607109053324962 1101 44-45 0.09206085120481333 1101 46-47 0.017737734769248448 1101 48-49 0.07964326667363508 1101 50-51 0.029609834480972097 1101 52-53 0.06522053224740176 1101 54-55 0.10684051124483318 1101 56-57 0.0024285970037425386 1101 58-59 -0.0029390703229239534 1101 60-61 -0.08466619587226631 1101 62-63 -0.21796524875920653 1101 64-65 -0.13564919201488834 1101 66-67 -0.18586647398539924 1101 68-69 -0.12221198343194573 1101 70-71 -0.211399719650899 1101 72-73 -0.18576184174053267 1101 74-75 -0.2065003451916887 1101 76-77 -0.2581625452548302 1101 78-79 -0.22559714840603107 1101 80-81 0.021165531680026106 1101 82-83 -0.19491297424103493 1101 84-85 -0.3060879494914239 1101 86-87 -0.34852590314420695 1101 88-89 -0.5378988052769351 1101 90-91 -0.39689771591765677 1101 92-93 -0.39684261630780426 1101 94-95 -0.07303881750771168 1101 96-97 -0.1330153049596028 1101 98-99 0.13126742078414466 1101 100-101 0.3495914247850678 1102 1 0.2948134439155936 1102 2 0.21427425762330898 1102 3 0.23471108986350941 1102 4 0.21888015422506868 1102 5 0.1773585594357101 1102 6 0.1461290174059613 1102 7 0.17805674252647918 1102 8 0.10288653431057782 1102 9 0.2974188180303514 1102 10-11 0.19012520352919182 1102 12-13 0.30076729457548623 1102 14-15 0.2524449974584684 1102 16-17 0.2716143949380978 1102 18-19 0.3380195536425745 1102 20-21 0.28837202960821173 1102 22-23 0.25680491942827643 1102 24-25 0.20079276020484826 1102 26-27 0.16918579869498274 1102 28-29 0.34020188390236683 1102 30-31 0.506331116841146 1102 32-33 0.35641331348844574 1102 34-35 0.4066845369289851 1102 36-37 0.3240288149585595 1102 38-39 0.2914457013292875 1102 40-41 0.45549479456398245 1102 42-43 0.48815654688254284 1102 44-45 0.5230955661632706 1102 46-47 0.7732673028636299 1102 48-49 0.7010485423776487 1102 50-51 0.6362276960970021 1102 52-53 0.47174367181204246 1102 54-55 0.5906171153470652 1102 56-57 0.7153809396910589 1102 58-59 0.6456308960529924 1102 60-61 0.49874981350117764 1102 62-63 0.5784513621666107 1102 64-65 0.605192285469542 1102 66-67 0.7668566727619535 1102 68-69 0.7030224648682193 1102 70-71 0.6939377108226665 1102 72-73 0.6959725145310962 1102 74-75 0.8192446370086444 1102 76-77 0.7779171290090545 1102 78-79 0.9074042836477325 1102 80-81 1.021697501494664 1102 82-83 0.9882007999613478 1102 84-85 0.77245110876488 1102 86-87 0.6962549567165404 1102 88-89 0.6511951401434075 1102 90-91 0.6286082165558433 1102 92-93 0.812321405473778 1102 94-95 1.053439811671172 1102 96-97 1.2253909404109145 1102 98-99 1.218681059861705 1102 100-101 1.4728013575817904 1103 1 0.15756688232878702 1103 2 0.10518037399342717 1103 3 0.10124085935725446 1103 4 0.05935865497536952 1103 5 0.12805470412548914 1103 6 0.15122712077836553 1103 7 0.21768903822437125 1103 8 0.13739195566428464 1103 9 0.12467790345193208 1103 10-11 0.09902192138186194 1103 12-13 0.15356061745348626 1103 14-15 0.28105321580193987 1103 16-17 0.2742432719675776 1103 18-19 0.2339859762261014 1103 20-21 0.3510859784522893 1103 22-23 0.2978453003333783 1103 24-25 0.10625303933968411 1103 26-27 0.1216522043543975 1103 28-29 0.09102778003351375 1103 30-31 0.08501098054571088 1103 32-33 0.0161266192166849 1103 34-35 0.05529619650633322 1103 36-37 0.020142195152850206 1103 38-39 0.1223807014985212 1103 40-41 0.135366961187394 1103 42-43 0.19858122899150743 1103 44-45 0.2376079828120723 1103 46-47 0.3525234322088906 1103 48-49 0.19578599315671852 1103 50-51 0.17469476743772105 1103 52-53 0.3288955148466428 1103 54-55 0.20261658505857127 1103 56-57 0.27280244006902876 1103 58-59 0.3527232226655741 1103 60-61 0.38419565073645856 1103 62-63 0.2651442235807835 1103 64-65 0.20653916184462418 1103 66-67 0.16514564614598015 1103 68-69 0.23073003896757172 1103 70-71 0.21199121354744577 1103 72-73 0.23813270678888543 1103 74-75 0.08629200192611108 1103 76-77 0.3129708912367164 1103 78-79 0.24511061664634326 1103 80-81 0.5405565914293398 1103 82-83 0.5329500976111561 1103 84-85 0.48301428643409494 1103 86-87 0.5523938923034102 1103 88-89 0.23609094409462372 1103 90-91 0.14438119666438354 1103 92-93 0.4662263597222882 1103 94-95 0.5645905377151053 1103 96-97 0.575732243115386 1103 98-99 0.8847674179079839 1103 100-101 0.8426417633089045 1104 1 0.1459156058486144 1104 2 0.05774842275919667 1104 3 0.07992092453921629 1104 4 0.12174624626030095 1104 5 0.058131956826315445 1104 6 0.0677247971619721 1104 7 0.005551914680403058 1104 8 0.10553125194046231 1104 9 0.09365309809252409 1104 10-11 0.02665786802921133 1104 12-13 0.1748111455481265 1104 14-15 0.2228043776101316 1104 16-17 0.16451275111538877 1104 18-19 0.14597372443056855 1104 20-21 0.10973465972455188 1104 22-23 0.24218714353453663 1104 24-25 0.2099044365272036 1104 26-27 0.229885954128072 1104 28-29 0.09686548900810976 1104 30-31 0.3057258697967242 1104 32-33 0.27021859821360295 1104 34-35 0.22914344136118103 1104 36-37 0.3433708148643646 1104 38-39 0.4709261779907692 1104 40-41 0.32566812601327655 1104 42-43 0.3549998420641103 1104 44-45 0.22657985800067593 1104 46-47 0.40958330305203106 1104 48-49 0.44791044242946043 1104 50-51 0.45095615919341014 1104 52-53 0.49032499158030163 1104 54-55 0.21343045433251717 1104 56-57 0.2695010941821323 1104 58-59 0.15835874683722295 1104 60-61 0.3743821435845476 1104 62-63 0.26377117753093415 1104 64-65 0.3889671900901419 1104 66-67 0.2708249267302065 1104 68-69 0.40367952294367626 1104 70-71 0.4166686231678227 1104 72-73 0.3068257480104428 1104 74-75 0.31576270021810515 1104 76-77 0.30629426621408484 1104 78-79 0.45295206754863315 1104 80-81 0.48516248194777845 1104 82-83 0.4053337659073719 1104 84-85 0.2649443968813152 1104 86-87 0.43304903141862283 1104 88-89 0.25823319770075415 1104 90-91 0.3061033887097686 1104 92-93 0.1809933945463591 1104 94-95 0.1945259592855102 1104 96-97 0.15821911619161355 1104 98-99 0.4582917706148457 1104 100-101 0.8420397354255087 1105 1 0.2161126501835895 1105 2 0.07493506753204571 1105 3 0.11054489826663882 1105 4 0.10258915813769676 1105 5 0.15465631369550437 1105 6 0.1341998929474144 1105 7 0.1371337428139583 1105 8 0.036675203774066745 1105 9 0.10982715392174924 1105 10-11 0.13374599063457993 1105 12-13 0.26640939404292396 1105 14-15 0.27314646846947 1105 16-17 0.3735302661673856 1105 18-19 0.30499123948256823 1105 20-21 0.34813093777599846 1105 22-23 0.1340590647168014 1105 24-25 0.3056077753339892 1105 26-27 0.3858383350804502 1105 28-29 0.2660909953025765 1105 30-31 0.2613772107928938 1105 32-33 0.2915650678359327 1105 34-35 0.2965353406496334 1105 36-37 0.4482285050668793 1105 38-39 0.47607122451512396 1105 40-41 0.6024606821161811 1105 42-43 0.586444833853939 1105 44-45 0.5053291738189571 1105 46-47 0.39473655975701405 1105 48-49 0.6251135075635617 1105 50-51 0.457866394551921 1105 52-53 0.5200868963422138 1105 54-55 0.43810204710755585 1105 56-57 0.4209515594257027 1105 58-59 0.5815525070999428 1105 60-61 0.506648153436764 1105 62-63 0.5084071928566019 1105 64-65 0.607686401912801 1105 66-67 0.3637368041248479 1105 68-69 0.34399150980738114 1105 70-71 0.31111306761226487 1105 72-73 0.5356571656349658 1105 74-75 0.3498896843450865 1105 76-77 0.23236158969520204 1105 78-79 0.6334802558354404 1105 80-81 0.7023080758175091 1105 82-83 0.5285275261700946 1105 84-85 0.3170790438435489 1105 86-87 0.37523567619147613 1105 88-89 0.45915000120376703 1105 90-91 0.354721341638065 1105 92-93 0.584017477742858 1105 94-95 0.5887240983112356 1105 96-97 0.7971764232523633 1105 98-99 0.9204017870352068 1105 100-101 1.099305745277725 1106 1 0.09299801896032278 1106 2 0.12268571853456933 1106 3 0.13566310749865096 1106 4 0.07997141319173551 1106 5 0.02489871777534347 1106 6 0.11729988078913323 1106 7 0.07264608079816526 1106 8 0.23678649881816227 1106 9 0.19183009061450917 1106 10-11 0.14477428736613263 1106 12-13 0.14509412177088876 1106 14-15 0.29435532237100404 1106 16-17 0.22305014763081488 1106 18-19 0.24909053122923552 1106 20-21 0.11937018738285587 1106 22-23 0.20609120800816783 1106 24-25 0.10405118120283419 1106 26-27 0.1450889840788676 1106 28-29 0.17878684719065063 1106 30-31 0.015274541448732748 1106 32-33 0.09835025771222661 1106 34-35 0.12951895636241062 1106 36-37 0.2206755664368032 1106 38-39 0.2251159309193369 1106 40-41 0.2926202228270469 1106 42-43 0.18491188824955174 1106 44-45 0.14026339216834316 1106 46-47 0.06052709526921518 1106 48-49 0.2501273693412571 1106 50-51 -0.09399101685628608 1106 52-53 -0.0711119102756399 1106 54-55 0.028081351306433078 1106 56-57 -0.15113770107016933 1106 58-59 -0.04272517737526016 1106 60-61 -0.0888554866994653 1106 62-63 -0.187874243317097 1106 64-65 -0.11386321790706688 1106 66-67 -0.06817180567579584 1106 68-69 -0.15481170104795794 1106 70-71 0.04067446092467719 1106 72-73 0.12403518399530711 1106 74-75 0.26630450088195445 1106 76-77 -0.014205365697264938 1106 78-79 -0.15728305714947055 1106 80-81 0.2572959108550066 1106 82-83 0.21820040157709997 1106 84-85 0.10176488585802801 1106 86-87 0.07899779606145074 1106 88-89 -0.07863152649106553 1106 90-91 -0.09814093174692218 1106 92-93 0.11321438980641219 1106 94-95 0.2367231256486626 1106 96-97 0.1563932027505608 1106 98-99 -0.05032247247864774 1106 100-101 0.31967060062757113 1107 1 0.1374454696278704 1107 2 0.10776976789056647 1107 3 0.0891944138860552 1107 4 0.1291006142503619 1107 5 0.19034952192897237 1107 6 0.11019966812095561 1107 7 0.1664515149443062 1107 8 0.17029238051576812 1107 9 0.1279818907342758 1107 10-11 0.1833854653436049 1107 12-13 0.17299098798224577 1107 14-15 0.21110239445338408 1107 16-17 0.21986940885590656 1107 18-19 0.28685256170304996 1107 20-21 0.3098763137781759 1107 22-23 0.21120565558483406 1107 24-25 0.28323854604713716 1107 26-27 0.3342025218309459 1107 28-29 0.34446610219551843 1107 30-31 0.2636264791032019 1107 32-33 0.3351563092393377 1107 34-35 0.3974934627063149 1107 36-37 0.47949744970737385 1107 38-39 0.4420772627115426 1107 40-41 0.5031315482199759 1107 42-43 0.49339779293150343 1107 44-45 0.42975737187501295 1107 46-47 0.659966545183444 1107 48-49 0.6868885927768034 1107 50-51 0.5707363043403078 1107 52-53 0.3923362850952614 1107 54-55 0.3453229912983957 1107 56-57 0.4029935457675009 1107 58-59 0.41243081576259044 1107 60-61 0.24948983947464143 1107 62-63 0.38611564914190666 1107 64-65 0.3585154494916978 1107 66-67 0.3921622962338418 1107 68-69 0.5553153361931749 1107 70-71 0.46971709602069467 1107 72-73 0.43689812741045486 1107 74-75 0.35166521118173577 1107 76-77 0.4729871291783425 1107 78-79 0.3226235465721885 1107 80-81 0.2232179404800121 1107 82-83 0.16051772213045012 1107 84-85 0.11754656244191963 1107 86-87 0.32109866855138947 1107 88-89 0.01468815947356461 1107 90-91 0.11180738995166806 1107 92-93 0.13050536722458972 1107 94-95 0.1116563974428395 1107 96-97 0.2103643018942556 1107 98-99 0.21486121991045692 1107 100-101 0.6130462553929092 1108 1 0.07509138574961582 1108 2 0.07015649100932819 1108 3 0.030991489751606593 1108 4 0.06894064913560527 1108 5 0.048227629798702765 1108 6 -0.0017397923267878923 1108 7 0.018177421504141478 1108 8 0.07041236420271701 1108 9 0.026681377897595837 1108 10-11 0.13449588908238042 1108 12-13 0.07267129417378015 1108 14-15 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-0.7294985818615274 2316 72-73 -0.8443682014876615 2316 74-75 -0.7824693861977927 2316 76-77 -0.7383108187082854 2316 78-79 -0.8604366650414121 2316 80-81 -0.8672332319906033 2316 82-83 -0.956021345064805 2316 84-85 -1.0502061710044828 2316 86-87 -0.7693623124040982 2316 88-89 -0.7407256783368936 2316 90-91 -1.0146748058649244 2316 92-93 -1.1197090819219824 2316 94-95 -1.1353132265711814 2316 96-97 -1.2358753925090262 2316 98-99 -1.4704594016264814 2316 100-101 -1.747074050132479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 7.0 5 19.0 6 66.0 7 186.0 8 367.0 9 831.0 10 1354.0 11 2445.0 12 3792.0 13 5389.0 14 6803.0 15 9591.0 16 13271.0 17 18620.0 18 16291.0 19 8770.0 20 7217.0 21 7322.0 22 7299.0 23 7824.0 24 8726.0 25 10333.0 26 12701.0 27 15278.0 28 18731.0 29 23534.0 30 29061.0 31 36214.0 32 45979.0 33 61082.0 34 83834.0 35 121006.0 36 183038.0 37 267213.0 38 193318.0 39 14123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01621851982242 19.745314779621637 15.403726908225831 20.83473979233012 2 15.558082854229458 22.05929021210515 39.430262396963805 22.952364536701587 3 18.678906679167653 27.1107094471052 30.988592849628915 23.22179102409823 4 12.689980437179507 17.892128239966304 37.70179947343878 31.716091849415413 5 13.007317436601681 38.62092295548727 33.330299503445275 15.04146010446577 6 29.588238799911732 37.795032706690016 17.433688615559074 15.183039877839184 7 27.037261948291054 35.01862855799015 20.977463713373098 16.966645780345704 8 26.718893207542916 33.42299954415201 20.098063693077393 19.760043555227675 9 27.683283654454065 16.379130521624205 20.800698430544738 35.136887393376995 10-11 24.629683918553013 27.297079190298007 26.90582309554606 21.16741379560292 12-13 26.481398183611265 25.27142540165644 27.900489634873892 20.346686779858405 14-15 23.38186047185904 25.784163229014485 27.659019266423012 23.174957032703468 16-17 20.934142423557848 29.326540712435918 27.91884775114234 21.8204691128639 18-19 20.77863501674878 26.779965576203928 32.023030276670994 20.418369130376302 20-21 22.01056764975099 25.744360490472324 32.09960861600514 20.14546324377154 22-23 21.701504942648526 25.74755847498715 32.00085048669423 20.55008609567009 24-25 21.483829760843317 25.991344836501497 32.25478043543221 20.27004496722298 26-27 21.334334765676914 26.055631848770293 32.313953066920114 20.296080318632683 28-29 20.840723432292197 25.980527189925944 32.59843128536978 20.58031809241208 30-31 20.94981473887406 26.221035577064843 32.287481439646925 20.54166824441417 32-33 20.915583819386388 26.485018934726334 32.007499794625474 20.591897451261808 34-35 21.409180696254147 26.067104417986066 31.81070779193321 20.71300709382658 36-37 21.533432626867093 26.04703259206871 31.5914540971471 20.828080683917104 38-39 21.320747346338962 26.184305855754086 31.814171035234885 20.68077576267207 40-41 21.078917618838773 26.403590485246315 31.827095131024603 20.690396764890313 42-43 21.150012524737363 26.761698950418953 31.020351288678683 21.067937236164997 44-45 21.226386790898992 26.936243882382264 30.60353246238619 21.233836864332552 46-47 21.742343275443957 27.026040957747977 29.89676398836682 21.334851778441248 48-49 22.04004051167127 27.338089226351915 29.32851703523831 21.29335322673851 50-51 22.035429270526226 27.572637092204776 29.101510510023154 21.29042312724584 52-53 21.922151936456082 27.88539446745243 28.90875307466668 21.28370052142481 54-55 21.61792764734261 27.702190618171684 28.951009694647205 21.7288720398385 56-57 21.858381207268838 28.07976456735466 28.517898663771547 21.543955561604953 58-59 21.963579341841232 27.422490024206077 28.85713583872175 21.75679479523095 60-61 21.925885239062467 27.55991662653164 29.26737336526954 21.246824769136353 62-63 21.93989894027425 27.456303829928437 29.35624761404656 21.247549615750756 64-65 21.921769296506817 27.541193025074644 29.231039368264877 21.305998310153658 66-67 21.71701368963844 27.944661564512852 28.90960086821181 21.4287238776369 68-69 21.53582975906239 27.902710029610304 29.08313557557675 21.47832463575055 70-71 21.837285836710763 27.92958304606403 28.716606957608715 21.516524159616488 72-73 21.767455684930763 27.942748953969843 28.55528423949006 21.734511121609337 74-75 21.712694633542853 28.418994107432237 28.249449887400086 21.618861371624824 76-77 21.791736839180302 28.09345005529232 28.491611112587723 21.62320199293966 78-79 21.65869442031358 28.04233123868048 28.48743993630258 21.811534404703366 80-81 21.64455880931671 28.76626880577301 27.995513997616055 21.593658387294223 82-83 21.55904280831641 28.50613678895946 27.892478028817557 22.042342373906575 84-85 21.460830304048027 28.192025076723777 27.93389654482275 22.41324807440545 86-87 21.673195655430472 28.180425597716575 28.022288228007753 22.124090518845207 88-89 21.741923065151873 27.984523470106037 27.76984460649054 22.503708858251546 90-91 21.499616027228843 27.955352040814276 28.093719155831735 22.45131277612515 92-93 21.476874654437637 28.09040978612483 27.993964412261086 22.438751147176447 94-95 21.40816053684097 27.928761615741838 27.89626411647877 22.766813730938424 96-97 21.452244093885735 27.958896199130663 27.664929443951813 22.92393026303179 98-99 21.35039141780226 28.24317032312103 27.475999484552688 22.930438774524017 100-101 21.092392475229186 28.451615111556762 27.560691267343596 22.895301145870455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2255.0 1 1578.0 2 953.5 3 1010.5 4 898.0 5 923.5 6 1533.5 7 2943.0 8 4522.5 9 5127.5 10 4449.5 11 3672.0 12 3532.0 13 3613.0 14 3768.0 15 3855.5 16 3886.0 17 3950.0 18 3988.0 19 3999.0 20 4123.0 21 4489.5 22 5116.0 23 5793.0 24 6372.0 25 7163.0 26 8301.5 27 9505.5 28 10637.5 29 12215.5 30 14261.5 31 16210.0 32 18686.0 33 21329.0 34 24032.0 35 26427.0 36 29852.0 37 33335.5 38 34669.0 39 34910.0 40 35011.0 41 36657.5 42 38278.5 43 40105.5 44 42795.5 45 44208.0 46 43542.5 47 44457.5 48 53322.0 49 52241.5 50 42243.5 51 38737.0 52 36428.0 53 36113.0 54 35509.5 55 33202.5 56 30714.0 57 27553.5 58 25361.5 59 23800.5 60 20919.5 61 17429.0 62 14177.0 63 11367.0 64 8434.0 65 6278.5 66 4727.0 67 3769.5 68 2754.5 69 1555.0 70 1007.0 71 670.0 72 464.0 73 363.5 74 258.0 75 179.0 76 115.0 77 52.5 78 33.0 79 20.0 80 10.5 81 6.0 82 4.0 83 4.0 84 2.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027785786885430746 2 0.007087389118602625 3 0.011275391779595086 4 8.053851271139346E-5 5 0.0070068506058912315 6 0.0 7 0.0 8 0.0 9 8.053851271139346E-5 10-11 0.0 12-13 2.0134628177848366E-4 14-15 0.0 16-17 4.026925635569673E-5 18-19 0.004751772249972214 20-21 0.0018121165360063527 22-23 0.0 24-25 0.005718234402508936 26-27 0.0070068506058912315 28-29 0.00934246747452164 30-31 0.03390671385149664 32-33 6.443081016911477E-4 34-35 0.005597426633441846 36-37 0.01651039510583566 38-39 0.00825519755291783 40-41 0.011154584010527994 42-43 0.007973312758427952 44-45 0.0035034253029456158 46-47 0.0016913087669392628 48-49 0.00197319356142914 50-51 0.0033423482775228283 52-53 0.0035436945593013123 54-55 0.00233561686863041 56-57 0.0010470006652481148 58-59 9.261928961810248E-4 60-61 0.0 62-63 0.0 64-65 0.008778697885541887 66-67 7.651158707582378E-4 68-69 0.0010872699216038118 70-71 0.005315541838951969 72-73 0.013087508315601439 74-75 0.00628200399148869 76-77 0.004751772249972214 78-79 0.011879430624930534 80-81 1.6107702542278693E-4 82-83 0.005839042171576026 84-85 0.0010067314088924183 86-87 0.0 88-89 0.0016107702542278693 90-91 0.0014094239724493857 92-93 5.235003326240574E-4 94-95 6.443081016911477E-4 96-97 4.0269256355696733E-4 98-99 1.6107702542278693E-4 100-101 7.651158707582378E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241642.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.8526881161405 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33577537946965 29.712243576271863 2 15.043958467790613 12.89348120504804 3 6.03793349663468 7.762250429918508 4 2.978645719701576 5.1057190413939395 5 1.6790795937971916 3.5976537077583464 6 1.067442397689246 2.744566569007348 7 0.718487380274756 2.1552380935607864 8 0.4882470232712801 1.6738157929502526 9 0.3744633973852001 1.4442086861152528 >10 2.00340575952326 15.784797236674825 >50 0.17351252272922638 5.110353413173149 >100 0.09302307783155625 7.189843820415496 >500 0.0037661149386788305 1.1209213880234081 >1k 0.0016947517224054735 1.4228786145717915 >5k 3.76611493867883E-4 1.3033706903285642 >10k+ 1.883057469339415E-4 0.9786577347885606 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12128 0.9767710821637798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9394 0.7565787884108301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6758 0.544279268903597 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2578 0.20762828576997233 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2462 0.19828581829545072 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2372 0.19103735215142528 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2206 0.17766795904133398 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.16607041321089333 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1825 0.14698278569829307 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1750 0.14094239724493854 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1348 0.10856591513495838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.026925635569673E-5 0.0 20-21 0.0 0.0 0.0 8.053851271139346E-5 0.0 22-23 0.0 0.0 0.0 4.0269256355696733E-4 0.0 24-25 0.0 0.0 0.0 9.664621525367215E-4 0.0 26-27 0.0 0.0 0.0 0.00197319356142914 0.0 28-29 0.0 0.0 0.0 0.004510156711838034 0.0 30-31 0.0 0.0 0.0 0.009745160038078609 0.0 32-33 0.0 0.0 0.0 0.014335855262628035 0.0 34-35 0.0 0.0 0.0 0.0196916663579357 0.0 36-37 0.0 0.0 0.0 0.023678322737149676 0.0 38-39 0.0 0.0 0.0 0.026819324732894022 0.0 40-41 0.0 0.0 0.0 0.030765711855752303 0.0 42-43 0.0 0.0 0.0 0.03640340774554984 0.0 44-45 0.0 0.0 0.0 0.04095383371374357 0.0 46-47 0.0 0.0 0.0 0.0505781859827551 0.0 48-49 0.0 0.0 0.0 0.061450885198793215 0.0 50-51 0.0 0.0 0.0 0.06994769828984522 0.0 52-53 0.0 0.0 0.0 0.08678024744652646 0.0 54-55 0.0 0.0 0.0 0.11641842012431924 0.0 56-57 0.0 0.0 0.0 0.18407077080188977 0.0 58-59 0.0 0.0 0.0 0.26263608995185406 0.0 60-61 0.0 0.0 0.0 0.33137571055102844 0.0 62-63 0.0 0.0 0.0 0.41952511271364856 0.0 64-65 0.0 4.026925635569673E-5 0.0 0.5244265255202385 0.0 66-67 0.0 8.053851271139346E-5 0.0 0.6901345154239306 0.0 68-69 0.0 8.053851271139346E-5 0.0 0.8904740657935218 0.0 70-71 0.0 8.053851271139346E-5 0.0 1.0556585553645899 0.0 72-73 0.0 8.053851271139346E-5 0.0 1.2373534400414936 0.0 74-75 0.0 8.053851271139346E-5 0.0 1.4218671726632959 0.0 76-77 0.0 8.053851271139346E-5 0.0 1.637871463755253 0.0 78-79 0.0 8.053851271139346E-5 0.0 1.814895114694896 0.0 80-81 0.0 8.053851271139346E-5 0.0 1.9773412948337765 0.0 82-83 0.0 8.053851271139346E-5 0.0 2.122270348457929 0.0 84-85 0.0 8.053851271139346E-5 0.0 2.430088544040875 0.0 86-87 0.0 8.053851271139346E-5 0.0 2.8362442636444323 0.0 88-89 0.0 8.053851271139346E-5 0.0 3.2976896722243607 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13950 0.0 27.140144 1 GGTATCA 9970 0.0 27.063328 1 GTACATG 7725 0.0 25.15086 1 GAGTACT 8775 0.0 24.217276 12-13 TACATGG 8385 0.0 22.994743 2 ACATGGG 8265 0.0 22.75401 3 GTACTTT 9380 0.0 22.681513 14-15 TATCAAC 16855 0.0 22.287088 2 ATCAACG 16920 0.0 22.229536 3 TCAACGC 16960 0.0 22.176214 4 AGTACTT 9105 0.0 21.983505 12-13 CAACGCA 17155 0.0 21.951822 5 AACGCAG 17405 0.0 21.635641 6 CATGGGG 2865 0.0 21.38224 4 ATGGGAG 1750 0.0 20.623562 5 ACAGCGC 210 8.632269E-7 20.351377 8 TACTTTT 10010 0.0 19.973072 14-15 ACTTTTT 10810 0.0 19.901146 16-17 ACGCAGA 18850 0.0 19.876333 7 CGCAGAG 18870 0.0 19.779772 8 >>END_MODULE