FastQCFastQC Report
Thu 26 May 2016
SRR937077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274104
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114860.9014962671807011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81060.6362118006065439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.43371655689017535No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23330.18310907115902628No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18600.1459849431443587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18400.14441521257291398No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.13562472137282358No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.13374104468708992No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16670.13083704312991717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14320.11239270891544176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.10870384207254666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127350.028.9490451
GGTATCA88250.028.7473661
TATCACG700.0083797527.1509252
GTACATG81850.026.1195281
TATCGCT1109.458292E-525.916797
CTATACG1151.2792763E-424.7899744
GAGTACT76350.024.61274712-13
TACATGG86400.024.4169772
TATCAAC151500.024.3058992
ACATGGG84700.024.1216893
TCAACGC152100.024.0850644
ATCAACG152750.024.013683
CAACGCA153850.023.780225
CATGGGG29700.023.6770694
AACGCAG156000.023.5438546
GTACTTT81300.023.25709714-15
AGTACTT80850.022.1262412-13
ATCGCGT550.002881223621.58715224-25
ACGCAGA170400.021.5263487
CGCAGAG169900.021.4219048
Produced by FastQC (version 0.11.5)