FastQCFastQC Report
Thu 26 May 2016
SRR937077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274104
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121450.9532188895098045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.7285119582074933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65150.5113397336481166No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25350.1989633499306179No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.18930950691623288No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.18750431675907148No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23380.18350150380188746No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.15979857217307222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.141432724487169No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17480.13719445194426827No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13900.10909627471540784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147200.026.5655271
GGTATCA106800.024.9139841
TATAGAC2151.6661943E-924.2983113
ATATAGG4250.023.4694732
GAGTACT92100.023.256212-13
GATATAG4750.023.0070341
GTACATG78850.022.7177891
AGTACTT95700.022.50542412-13
TATCAAC176750.022.1163462
TCAACGC176900.022.0681444
ATCAACG177400.022.0059453
CAACGCA178400.021.886895
GTACTTT98500.021.60133714-15
CTTAACG550.002875834221.5941220-21
AACGCAG181550.021.5594676
TACATGG85700.020.839152
GGGCGTA3900.020.7057699
ACATGGG83100.020.4599173
TACTTTT105700.020.10744714-15
ACGCAGA196250.019.8477487
Produced by FastQC (version 0.11.5)