##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1274104 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19012105762167 33.0 31.0 34.0 31.0 34.0 2 32.29714450311749 33.0 31.0 34.0 31.0 34.0 3 32.39037629581259 34.0 31.0 34.0 31.0 34.0 4 36.061218707421055 37.0 35.0 37.0 35.0 37.0 5 35.97637241543861 37.0 35.0 37.0 35.0 37.0 6 36.033630692627916 37.0 35.0 37.0 35.0 37.0 7 35.97657726527819 37.0 35.0 37.0 35.0 37.0 8 35.943905678029424 37.0 35.0 37.0 35.0 37.0 9 37.61603212924533 39.0 37.0 39.0 35.0 39.0 10-11 37.529581180186234 39.0 37.0 39.0 35.0 39.0 12-13 37.436101762493486 39.0 37.0 39.0 34.5 39.0 14-15 38.58453352316609 40.0 38.0 41.0 34.0 41.0 16-17 38.517088087000744 40.0 38.0 41.0 34.0 41.0 18-19 38.71281543735833 40.0 38.0 41.0 34.5 41.0 20-21 38.75406010812304 40.0 38.0 41.0 34.5 41.0 22-23 38.66857336606745 40.0 38.0 41.0 34.0 41.0 24-25 38.557923842951595 40.0 38.0 41.0 34.0 41.0 26-27 38.35483798810772 40.0 38.0 41.0 34.0 41.0 28-29 38.24235815914557 40.0 38.0 41.0 34.0 41.0 30-31 38.16521963670156 40.0 37.5 41.0 34.0 41.0 32-33 37.922899935954995 40.0 37.0 41.0 33.0 41.0 34-35 37.73326863427161 40.0 36.5 41.0 33.0 41.0 36-37 37.53612656423651 40.0 36.0 41.0 33.0 41.0 38-39 37.34647760308421 40.0 36.0 41.0 33.0 41.0 40-41 37.110321056993776 40.0 36.0 41.0 32.0 41.0 42-43 36.794771855358746 39.0 35.0 41.0 31.0 41.0 44-45 36.41966354394931 39.0 35.0 41.0 30.5 41.0 46-47 35.894503117484916 39.0 35.0 41.0 29.5 41.0 48-49 35.40110736643163 39.0 35.0 40.0 27.0 41.0 50-51 35.06601187972096 39.0 35.0 40.0 26.0 41.0 52-53 34.79399248413003 38.0 34.5 40.0 25.0 41.0 54-55 34.49130879425856 38.0 34.0 40.0 24.0 41.0 56-57 34.19749486698103 38.0 34.0 40.0 23.0 41.0 58-59 33.83789078442577 37.5 33.5 40.0 22.5 41.0 60-61 33.50116513251666 37.0 33.0 40.0 22.0 41.0 62-63 33.122282011515544 36.5 33.0 39.0 20.5 41.0 64-65 32.72447264901452 36.0 32.0 39.0 19.5 40.0 66-67 32.33148118206991 35.0 32.0 39.0 18.0 40.0 68-69 32.03328927622863 35.0 31.5 38.0 17.5 40.0 70-71 31.585491058814668 35.0 31.0 37.5 13.0 40.0 72-73 31.100095832051387 35.0 31.0 37.0 8.0 39.0 74-75 30.598430740347727 34.5 30.0 36.5 7.0 39.0 76-77 29.19450727727093 33.0 28.5 35.0 6.5 37.0 78-79 29.931100993325508 34.0 30.0 35.0 2.0 37.5 80-81 29.71459825885485 34.0 30.0 35.0 2.0 37.0 82-83 29.40402941989037 34.0 29.5 35.0 2.0 37.0 84-85 29.058815057483535 34.0 29.0 35.0 2.0 36.0 86-87 28.703408434476305 34.0 29.0 35.0 2.0 36.0 88-89 28.314858520183595 34.0 29.0 35.0 2.0 35.0 90-91 27.86072055342421 33.0 27.5 35.0 2.0 35.0 92-93 27.39717676108073 33.0 27.0 35.0 2.0 35.0 94-95 26.83366349999686 33.0 25.5 35.0 2.0 35.0 96-97 26.187319873416925 32.5 25.0 34.0 2.0 35.0 98-99 25.412327015691027 32.0 22.0 34.0 2.0 35.0 100-101 22.041407922744142 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13344981699438563 1101 2 0.13815204709222684 1101 3 0.14301590948076637 1101 4 0.1475507358924375 1101 5 0.2038692763254275 1101 6 0.22621564905151814 1101 7 0.1962312678586997 1101 8 0.19014446946435726 1101 9 0.2430218591535791 1101 10-11 0.32930974042638184 1101 12-13 0.22353915831686066 1101 14-15 0.3247715472088686 1101 16-17 0.4348265109265128 1101 18-19 0.2966496634522855 1101 20-21 0.32879961842002814 1101 22-23 0.31426694593501026 1101 24-25 0.39031283416670703 1101 26-27 0.22443047378837377 1101 28-29 0.33124993270736525 1101 30-31 0.4905642576875664 1101 32-33 0.6321478164343333 1101 34-35 0.5437955283038676 1101 36-37 0.6045764173317494 1101 38-39 0.5983350025065235 1101 40-41 0.5314061300027575 1101 42-43 0.700972104864185 1101 44-45 0.5778884113330633 1101 46-47 0.6679950763581886 1101 48-49 0.49657278247216396 1101 50-51 0.5288646696292005 1101 52-53 0.5121540599190197 1101 54-55 0.5467028730311725 1101 56-57 0.5160973593808862 1101 58-59 0.48976255087723786 1101 60-61 0.5775291312340514 1101 62-63 0.3679042687724632 1101 64-65 0.3797403051590038 1101 66-67 0.31104953521002443 1101 68-69 0.3208253689944698 1101 70-71 0.2357903977561655 1101 72-73 0.45221788275599906 1101 74-75 0.45290141937600126 1101 76-77 0.33924162039683026 1101 78-79 0.24151704968334897 1101 80-81 0.5387806590710973 1101 82-83 0.7706211459970369 1101 84-85 0.6540259018382635 1101 86-87 -0.016001719856955532 1101 88-89 0.16522688590072576 1101 90-91 0.2852019758461459 1101 92-93 0.21885205329855495 1101 94-95 0.25545956655304636 1101 96-97 0.24029659276567017 1101 98-99 0.298071423616161 1101 100-101 0.8242248189694621 1102 1 0.2914577751874603 1102 2 0.32722358418995867 1102 3 0.36283088043526135 1102 4 0.24850102782668415 1102 5 0.27134697711343136 1102 6 0.28235725670795375 1102 7 0.18311377356953784 1102 8 0.25604152011489134 1102 9 0.2500037683169083 1102 10-11 0.254126370218259 1102 12-13 0.30975168391261576 1102 14-15 0.39021162514252694 1102 16-17 0.4867623847111844 1102 18-19 0.3468918325776258 1102 20-21 0.31275063094478384 1102 22-23 0.2558901341570561 1102 24-25 0.3180440517956029 1102 26-27 0.3833021896601707 1102 28-29 0.43924288740314665 1102 30-31 0.3818195432506073 1102 32-33 0.39509467235638596 1102 34-35 0.28658698180465336 1102 36-37 0.30265375085103585 1102 38-39 0.3650966561032405 1102 40-41 0.4043809220141341 1102 42-43 0.4663137091395413 1102 44-45 0.40227934330382453 1102 46-47 0.45887379527083283 1102 48-49 0.4700873834108279 1102 50-51 0.45678501585074116 1102 52-53 0.5356804946020404 1102 54-55 0.6340005107199573 1102 56-57 0.58276043366088 1102 58-59 0.6240153238827801 1102 60-61 0.5790426882674211 1102 62-63 0.6991603573395864 1102 64-65 0.43494366594239153 1102 66-67 0.5576853707463343 1102 68-69 0.5256553328804117 1102 70-71 0.3809962520785426 1102 72-73 0.5098820284124876 1102 74-75 0.6552262218475633 1102 76-77 0.3451526813554082 1102 78-79 0.29466007104959857 1102 80-81 0.5599894760559003 1102 82-83 0.6335985372058346 1102 84-85 0.664663592736634 1102 86-87 0.4879551884082822 1102 88-89 0.43304987844335585 1102 90-91 0.7350274281170606 1102 92-93 0.6511598658943072 1102 94-95 0.3707397832796211 1102 96-97 0.6191422955315957 1102 98-99 0.8884247031482211 1102 100-101 0.8848780416572879 1103 1 0.21984236757685238 1103 2 0.1910496814678666 1103 3 0.1949299959307993 1103 4 0.13613774795047817 1103 5 0.15255873064793235 1103 6 0.20130442383182157 1103 7 0.06519266679595859 1103 8 0.0751236489043734 1103 9 0.09939512197802003 1103 10-11 0.23270010676605324 1103 12-13 0.23853028083883032 1103 14-15 0.2566864000598841 1103 16-17 0.29353091006814225 1103 18-19 0.3122155867100034 1103 20-21 0.19256386070492937 1103 22-23 0.35675749365624654 1103 24-25 0.20942463929648625 1103 26-27 0.2926641111274293 1103 28-29 0.3043460649370857 1103 30-31 0.39612130346725394 1103 32-33 0.300296191664259 1103 34-35 0.42914353821598894 1103 36-37 0.28723372113309864 1103 38-39 0.29546610816767327 1103 40-41 0.3746052792976684 1103 42-43 0.49747029104852913 1103 44-45 0.5830488446050808 1103 46-47 0.29897939068477797 1103 48-49 0.4025098354049206 1103 50-51 0.4003951085214936 1103 52-53 0.3783803653553619 1103 54-55 0.3526054379197845 1103 56-57 0.4421684047518255 1103 58-59 0.3116988629557156 1103 60-61 0.3172082646900165 1103 62-63 0.29289277275775305 1103 64-65 0.2112853695366823 1103 66-67 0.24942929966945826 1103 68-69 0.2335578439586996 1103 70-71 0.26338562307805446 1103 72-73 0.3194421061363393 1103 74-75 0.39469965154440345 1103 76-77 0.17675503241400037 1103 78-79 0.3196691930514284 1103 80-81 0.13041187969239232 1103 82-83 0.024754921288270282 1103 84-85 0.13305365832712468 1103 86-87 0.1616787675740703 1103 88-89 0.10337194005973416 1103 90-91 0.13683951927553295 1103 92-93 0.13059140032491712 1103 94-95 -0.055961085398724464 1103 96-97 0.011936243797755708 1103 98-99 0.04447412901162551 1103 100-101 0.24469155716688817 1104 1 0.23221272983218455 1104 2 0.2665727971558596 1104 3 0.20846435906255323 1104 4 0.09429762232967676 1104 5 0.12391061495993938 1104 6 0.0579132099843207 1104 7 0.16821359142347347 1104 8 0.12661813473551575 1104 9 0.14564019209195322 1104 10-11 0.2936321291148687 1104 12-13 0.2306812631642785 1104 14-15 0.280327029624587 1104 16-17 0.3314590798023005 1104 18-19 0.2805387863886466 1104 20-21 0.19069917694799443 1104 22-23 0.17216977820196888 1104 24-25 0.24026609781240893 1104 26-27 0.0872225211478792 1104 28-29 0.1681488329768186 1104 30-31 0.2722396572504806 1104 32-33 0.3210564896484769 1104 34-35 0.1400992970532613 1104 36-37 0.11851065291808283 1104 38-39 0.10058953790766623 1104 40-41 -9.812858498605692E-4 1104 42-43 -0.016146236850971718 1104 44-45 0.12296266289312996 1104 46-47 0.1728382133549502 1104 48-49 0.06585339368712795 1104 50-51 0.08911616826441104 1104 52-53 -0.04258397513543599 1104 54-55 -0.06690047794349141 1104 56-57 -0.07964634108515867 1104 58-59 0.12802865407461894 1104 60-61 0.18139903302188287 1104 62-63 0.2899439705374789 1104 64-65 -0.009062278725075146 1104 66-67 0.0680528572213106 1104 68-69 -0.030629272605718683 1104 70-71 -0.09012600780129176 1104 72-73 0.06068242895257825 1104 74-75 0.09100954053067412 1104 76-77 -0.3125407242322211 1104 78-79 -0.292332797161837 1104 80-81 0.10081658682064187 1104 82-83 -0.11021212151044324 1104 84-85 0.123314850994376 1104 86-87 -0.03242636681097366 1104 88-89 0.0021175579387850973 1104 90-91 0.10709587335090376 1104 92-93 -0.0606653554448755 1104 94-95 0.08206659499859015 1104 96-97 0.0689700043878787 1104 98-99 0.24398616158539355 1104 100-101 0.5608341952036966 1105 1 0.18078075124385862 1105 2 0.25076966506701126 1105 3 0.16631862561069966 1105 4 0.1346072366960982 1105 5 0.14179213184689132 1105 6 0.21576298338390387 1105 7 0.21302485489685097 1105 8 0.11574228650192708 1105 9 0.1416078948543955 1105 10-11 0.11208221144877939 1105 12-13 0.16029305512906689 1105 14-15 0.256666959385754 1105 16-17 0.21451517372610596 1105 18-19 0.15931910299774898 1105 20-21 0.16173536986678982 1105 22-23 0.2444080705140692 1105 24-25 0.10465619539873217 1105 26-27 0.13022225641642393 1105 28-29 0.1498192155987752 1105 30-31 0.22743810869290115 1105 32-33 0.10817991522494452 1105 34-35 0.2053310099386394 1105 36-37 0.13939711469716798 1105 38-39 0.19442408803420363 1105 40-41 0.06735161031226511 1105 42-43 0.3147756722271282 1105 44-45 0.35388924727446636 1105 46-47 0.3094101960235349 1105 48-49 0.22099756303754248 1105 50-51 0.17204936850339436 1105 52-53 0.3686626792904306 1105 54-55 0.2692544312463312 1105 56-57 0.3109102750153667 1105 58-59 0.1985559068421523 1105 60-61 0.05165572538450647 1105 62-63 0.03731153194788561 1105 64-65 -0.103639412727766 1105 66-67 0.0756623686096276 1105 68-69 -0.03029805469529734 1105 70-71 -0.10528643749201905 1105 72-73 0.054255404971311094 1105 74-75 0.1357534067757129 1105 76-77 -0.06769023678631925 1105 78-79 0.07432876917403775 1105 80-81 0.37360322541120183 1105 82-83 0.5298368902397463 1105 84-85 0.42661755805566415 1105 86-87 0.1645259426673853 1105 88-89 0.19425885896030692 1105 90-91 0.3853620280415093 1105 92-93 -0.005459739252053453 1105 94-95 0.0356469057787443 1105 96-97 -0.20629150900170146 1105 98-99 -0.010785987208262071 1105 100-101 0.10472223808533698 1106 1 0.21248692539997904 1106 2 0.168179783508684 1106 3 0.1552403700804561 1106 4 0.1501258431885759 1106 5 0.1915893428928257 1106 6 0.20442942383181872 1106 7 0.14926154434697736 1106 8 0.13017892101321138 1106 9 0.157520121978024 1106 10-11 0.2850406679905433 1106 12-13 0.2717041753966569 1106 14-15 0.37110391706668366 1106 16-17 0.23441951551032503 1106 18-19 0.04370580779843891 1106 20-21 0.2772811226096863 1106 22-23 0.22033742562903313 1106 24-25 0.20077880596315367 1106 26-27 0.2683252505832172 1106 28-29 0.31794725541327296 1106 30-31 0.22007113339922313 1106 32-33 0.08356149778671096 1106 34-35 0.06433273889626179 1106 36-37 0.18286084698343785 1106 38-39 0.2901769925214097 1106 40-41 0.26296199698474965 1106 42-43 0.22437930465397216 1106 44-45 -0.017631427503758346 1106 46-47 -0.19580802428120592 1106 48-49 -0.28953098092161156 1106 50-51 -0.22706450032204373 1106 52-53 -0.24481478770586307 1106 54-55 -0.17711394983530937 1106 56-57 -0.008405574840004704 1106 58-59 -0.0875911030306824 1106 60-61 -0.0742883339494469 1106 62-63 -0.06500688710619329 1106 64-65 -0.12864660325242738 1106 66-67 0.0803625479687824 1106 68-69 0.05253743579543624 1106 70-71 -0.184992353112424 1106 72-73 -0.1990740503262458 1106 74-75 -0.2424517089998126 1106 76-77 -0.038733913164236355 1106 78-79 -0.06840266069006873 1106 80-81 -0.13988361350488532 1106 82-83 -0.05516642224914392 1106 84-85 -0.006757140992601052 1106 86-87 -0.10090201473885685 1106 88-89 -0.05741887626679798 1106 90-91 -0.14872170521426042 1106 92-93 0.08719429148138147 1106 94-95 0.23198959487338655 1106 96-97 0.30661524039639687 1106 98-99 0.1094634997599222 1106 100-101 -0.15201762650658068 1107 1 0.20371589347624308 1107 2 0.2211591194436835 1107 3 0.11695427194214147 1107 4 0.10137976168387297 1107 5 0.034136204897819766 1107 6 0.15767617707857084 1107 7 0.12967290638395212 1107 8 0.21168554247057614 1107 9 0.18412558548003943 1107 10-11 0.3517628866585696 1107 12-13 0.2574568463709994 1107 14-15 0.37213040289612564 1107 16-17 0.24100674601380945 1107 18-19 0.27759871292287386 1107 20-21 0.3156174414632531 1107 22-23 0.36969319301436343 1107 24-25 0.346978985094367 1107 26-27 0.38175273442941915 1107 28-29 0.4065231622650387 1107 30-31 0.30284478831573836 1107 32-33 0.30348696640675854 1107 34-35 0.2033180885220034 1107 36-37 0.39595213992694056 1107 38-39 0.4649783488005994 1107 40-41 0.5406316918219289 1107 42-43 0.41382207862876896 1107 44-45 0.29729723988796053 1107 46-47 0.20745920110424265 1107 48-49 0.27163209245821207 1107 50-51 0.3186606612159224 1107 52-53 0.4480561098694693 1107 54-55 0.5241095803291032 1107 56-57 0.6739404043039983 1107 58-59 0.8000530016331311 1107 60-61 0.5313447029643825 1107 62-63 0.5088904962985765 1107 64-65 0.5755748157226819 1107 66-67 0.6845583767705179 1107 68-69 0.5450782201342506 1107 70-71 0.503303661218073 1107 72-73 0.5464652625775948 1107 74-75 0.45565557780205523 1107 76-77 0.44691895613395616 1107 78-79 0.5321017856025527 1107 80-81 0.4425538505089328 1107 82-83 0.6729171936423555 1107 84-85 0.550421222515812 1107 86-87 0.6075312268963557 1107 88-89 0.4753071169518073 1107 90-91 0.7504478936597714 1107 92-93 0.774887657228934 1107 94-95 0.8439446841191156 1107 96-97 0.6844538303778407 1107 98-99 0.5525292025300601 1107 100-101 0.6043114631230004 1108 1 0.05222747278172335 1108 2 0.09333719531061746 1108 3 0.11863223845571014 1108 4 0.027645223033530897 1108 5 -0.006923550636678044 1108 6 0.03554791774101318 1108 7 0.07899734564423966 1108 8 0.1504731782509907 1108 9 0.1065345184342803 1108 10-11 -0.033625682270489676 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2316 70-71 -0.5927724651572426 2316 72-73 -0.27146300590848327 2316 74-75 -0.4897249516301976 2316 76-77 -0.32824905255332126 2316 78-79 -0.4933633116171059 2316 80-81 -0.3828861478519556 2316 82-83 -0.39231294752592305 2316 84-85 -0.6537912879847347 2316 86-87 -0.6478304527807417 2316 88-89 -0.6508005622745365 2316 90-91 -0.6101842902482737 2316 92-93 -0.6886050282465064 2316 94-95 -0.7389967996844398 2316 96-97 -0.5888676194141951 2316 98-99 -0.9003861067493482 2316 100-101 -0.8053492925063139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 9.0 5 43.0 6 70.0 7 208.0 8 430.0 9 898.0 10 1508.0 11 2697.0 12 3869.0 13 5643.0 14 7079.0 15 9950.0 16 13746.0 17 19383.0 18 16725.0 19 9028.0 20 7698.0 21 7513.0 22 7639.0 23 8528.0 24 9757.0 25 11187.0 26 13454.0 27 16650.0 28 20403.0 29 25518.0 30 31601.0 31 39340.0 32 49880.0 33 65607.0 34 89316.0 35 129828.0 36 192656.0 37 271525.0 38 174806.0 39 9907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96120474436395 19.690460713287546 15.341067890127214 21.00726665222129 2 15.587287159767632 21.969923525473327 39.45168792616123 22.991101388597816 3 18.64757974446296 27.13992061853809 30.936715378137215 23.27578425886174 4 12.728739715297296 17.93193789189397 37.55887484587171 31.78044754693702 5 12.98159333931924 38.562786083396645 33.438950038615374 15.016670538668741 6 29.627330265033308 37.67628074317324 17.48460094309413 15.21178804869932 7 26.98680798427758 35.04360711527473 21.03227052108776 16.93731437935993 8 26.675373438902945 33.4835303868444 20.064924056434954 19.776172117817698 9 27.7226409481808 16.37324454930253 20.73505831161217 35.1690561909045 10-11 24.579532127937153 27.29559837291925 26.934160061164675 21.190709437978928 12-13 26.52891757658715 25.261281653616972 27.8376411972649 20.372159572530972 14-15 23.385728323590538 25.718897358457394 27.573651758412186 23.321722559539882 16-17 20.998050394630265 29.285756892686937 27.848629311265015 21.86756340141778 18-19 20.80796577351403 26.767147973355332 32.002250998939644 20.422635254190993 20-21 22.109304851784177 25.740961377864007 31.927190272571437 20.222543497780375 22-23 21.76358973453342 25.726574717786004 31.953982391181068 20.555853156499506 24-25 21.55520162768445 26.003781917286062 32.108501307343964 20.332515147685516 26-27 21.327831118907877 26.11122557705553 32.16927354678943 20.391669757247165 28-29 20.90128647571896 25.936954762613013 32.49353858132126 20.66822018034677 30-31 21.035568478337225 26.226518536948905 32.17241526815579 20.565497716558085 32-33 21.01502427124455 26.41933505149177 31.957040544693115 20.60860013257056 34-35 21.45017204247063 26.057409756189447 31.697804888768893 20.79461331257103 36-37 21.58935037992588 26.053327378328937 31.47385966310007 20.88346257864511 38-39 21.42712203009983 26.165146760706552 31.707063392539997 20.700667816653617 40-41 21.142244263407424 26.376179822373725 31.7457034666989 20.735872447519952 42-43 21.159811130017644 26.721052598531987 30.932796694775313 21.18633957667506 44-45 21.328125490540803 26.83933179709035 30.528983505271157 21.303559207097695 46-47 21.741926951602327 26.98350718801225 29.82315759235633 21.45140826802909 48-49 22.05120376369679 27.28507499412315 29.24662880626718 21.41709243591288 50-51 22.10154056512767 27.511571702957504 28.97168239256585 21.41520533934897 52-53 21.966260211097367 27.84180098327923 28.806518149224868 21.385420656398534 54-55 21.669626370866307 27.628658832117075 28.856900100662646 21.84481469635397 56-57 21.91647551229046 28.032486759820497 28.429800067893325 21.621237659995725 58-59 21.970567459383094 27.43049996075661 28.752413468330584 21.84651911152971 60-61 21.987750266174857 27.430942684792853 29.22323250280499 21.3580745462273 62-63 21.97141454405456 27.401426721855955 29.29528480082446 21.33187393326502 64-65 21.9811592866351 27.503085505287057 29.107940687660527 21.40781452041731 66-67 21.73693104533585 27.90912423110522 28.82685317671178 21.52709154684715 68-69 21.60235837616759 27.821822043376603 29.043118381096573 21.532701199359234 70-71 21.957933063519526 27.82298874842682 28.646243961859792 21.572834226193862 72-73 21.81741050966012 27.850552231215037 28.467377859264236 21.86465939986061 74-75 21.752659123588028 28.329987190998533 28.20699880072584 21.71035488468759 76-77 21.839543710717493 28.00728982877379 28.441908980742546 21.71125747976617 78-79 21.71667759619456 27.95522072348373 28.480701436300865 21.847400244020843 80-81 21.584321377385475 28.680853268198035 28.010087389507294 21.724737964909195 82-83 21.6080703767751 28.366065043726447 27.915063703792086 22.11080087570637 84-85 21.55671185006511 28.06334100736817 27.84171498587512 22.5382321566916 86-87 21.68745813025567 28.06782829902189 27.98282315201126 22.26189041871118 88-89 21.8234661145388 27.844888278087844 27.823500648692683 22.50814495868067 90-91 21.561868298891092 27.88300978855881 28.04120118720074 22.513920725349358 92-93 21.552042144192537 27.94984232031658 27.9220188022623 22.576096733228578 94-95 21.421132026898903 27.77445954176425 27.840702171879215 22.963706259457627 96-97 21.439060897111375 27.939464722027964 27.63371157089855 22.987762809962113 98-99 21.40953240586308 28.143044954182443 27.415811471067247 23.03161116888723 100-101 21.17070042747805 28.32912317356441 27.46620066470424 23.033975734253303 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2170.0 1 1512.0 2 924.5 3 993.5 4 878.5 5 882.0 6 1469.5 7 2871.5 8 4435.5 9 5017.5 10 4325.0 11 3599.5 12 3524.0 13 3557.5 14 3701.0 15 3847.0 16 3870.0 17 3952.5 18 3992.5 19 4042.0 20 4320.0 21 4632.5 22 5137.5 23 5840.5 24 6390.0 25 7267.0 26 8502.5 27 9674.5 28 10676.0 29 12269.5 30 14525.0 31 16632.5 32 18945.0 33 21499.0 34 24411.5 35 26997.5 36 30583.5 37 34154.5 38 35582.5 39 35782.0 40 36047.0 41 37710.5 42 39352.0 43 41277.0 44 43637.0 45 45494.5 46 45097.5 47 45987.5 48 55171.0 49 53717.0 50 43565.5 51 39889.5 52 37316.5 53 37512.0 54 37079.5 55 34299.0 56 31501.0 57 28278.0 58 26111.0 59 24609.0 60 21881.0 61 18199.5 62 14737.5 63 11749.5 64 8600.0 65 6383.0 66 4764.0 67 3863.5 68 2917.5 69 1714.5 70 1078.0 71 697.0 72 464.5 73 355.5 74 280.5 75 204.0 76 126.0 77 59.5 78 25.0 79 15.0 80 11.0 81 7.0 82 7.0 83 3.0 84 1.0 85 2.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026685419714560194 2 0.0066713549286400485 3 2.354595857167076E-4 4 2.354595857167076E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 7.848652857223586E-5 10-11 0.013028763742991153 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.354595857167076E-4 20-21 0.0012557844571557738 22-23 0.0020798930071642503 24-25 0.011537519700118671 26-27 0.0056117867929148645 28-29 2.354595857167076E-4 30-31 0.003178704407175552 32-33 0.005140867621481449 34-35 0.0 36-37 0.008280328764370883 38-39 0.013852872292999628 40-41 0.0019229199500197787 42-43 0.0 44-45 0.0 46-47 2.747028500028255E-4 48-49 0.0029432448214588448 50-51 0.007142274100073463 52-53 0.0 54-55 0.0036496235786089674 56-57 0.0036496235786089674 58-59 3.1394611428894344E-4 60-61 0.002786271764314373 62-63 0.005062381092909214 64-65 1.177297928583538E-4 66-67 0.0027470285000282552 68-69 5.49405700005651E-4 70-71 0.0012165411928696558 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0025508121785976657 80-81 0.002982488085744963 82-83 0.012636331100129974 84-85 0.00871200467151818 86-87 0.004983894564336977 88-89 2.354595857167076E-4 90-91 0.0011380546642974201 92-93 1.5697305714447172E-4 94-95 0.0 96-97 0.0029040015571727266 98-99 0.0022761093285948403 100-101 2.747028500028255E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1274104.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.527681745051254 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15534172940143 29.410163640382464 2 15.062312270273223 12.811304451495204 3 6.040634918974715 7.706825981166005 4 3.0303495669856924 5.154949678440856 5 1.6933207563533725 3.6006503109242853 6 1.0895428611381142 2.780143922764457 7 0.7131697486321068 2.1230619270017104 8 0.5238971034199209 1.782410342511728 9 0.37218520290947477 1.424535647359632 >10 2.0392296187395753 15.961893357943985 >50 0.18146588751908718 5.281024189638532 >100 0.09206676409113904 7.124031577890512 >500 0.004260632740870413 1.2421299720158538 >1k 0.0016672041159927703 1.3955941177307574 >5k 3.7048980355394896E-4 1.2444933828841531 >10k+ 1.8524490177697448E-4 0.9567874998498476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12145 0.9532188895098045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9282 0.7285119582074933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6515 0.5113397336481166 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2535 0.1989633499306179 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2412 0.18930950691623288 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2389 0.18750431675907148 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2338 0.18350150380188746 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2036 0.15979857217307222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.141432724487169 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1748 0.13719445194426827 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1390 0.10909627471540784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.848652857223586E-5 0.0 10-11 0.0 0.0 0.0 7.848652857223586E-5 0.0 12-13 0.0 0.0 0.0 7.848652857223586E-5 0.0 14-15 0.0 0.0 0.0 7.848652857223586E-5 0.0 16-17 0.0 0.0 0.0 1.5697305714447172E-4 0.0 18-19 0.0 0.0 0.0 1.5697305714447172E-4 0.0 20-21 0.0 0.0 0.0 1.5697305714447172E-4 0.0 22-23 0.0 0.0 0.0 4.709191714334152E-4 0.0 24-25 0.0 0.0 0.0 8.241085500084765E-4 0.0 26-27 0.0 0.0 0.0 0.0014127575143002453 0.0 28-29 0.0 0.0 0.0 0.003571137050036732 0.0 30-31 0.0 0.0 0.0 0.00777016632865135 0.0 32-33 0.0 0.0 0.0 0.012597087835843856 0.0 34-35 0.0 0.0 0.0 0.017777198721611424 0.0 36-37 0.0 0.0 0.0 0.022486390435945573 0.0 38-39 0.0 0.0 0.0 0.027117095621707492 0.0 40-41 0.0 0.0 0.0 0.03186553060032776 0.0 42-43 0.0 0.0 0.0 0.0383014259432511 0.0 44-45 0.0 0.0 0.0 0.04379548294330761 0.0 46-47 0.0 0.0 0.0 0.05258597414339803 0.0 48-49 0.0 0.0 0.0 0.06377030446494164 0.0 50-51 0.0 0.0 0.0 0.07169744385073745 0.0 52-53 0.0 0.0 0.0 0.0875517226223291 0.0 54-55 0.0 0.0 0.0 0.12306687680126582 0.0 56-57 0.0 0.0 0.0 0.19138939992339715 0.0 58-59 0.0 0.0 0.0 0.26948349585277187 0.0 60-61 0.0 0.0 0.0 0.3407100205320759 0.0 62-63 0.0 0.0 0.0 0.4244159032543654 0.0 64-65 0.0 0.0 0.0 0.5315500147554674 0.0 66-67 0.0 0.0 0.0 0.6923689117999786 0.0 68-69 0.0 0.0 0.0 0.894157776759197 0.0 70-71 0.0 0.0 0.0 1.0595288924608979 0.0 72-73 0.0 0.0 0.0 1.2367514739770065 0.0 74-75 0.0 0.0 0.0 1.4255115751932337 0.0 76-77 0.0 0.0 0.0 1.6374252023382705 0.0 78-79 0.0 0.0 0.0 1.812881836961504 0.0 80-81 0.0 0.0 0.0 1.9742108964417349 0.0 82-83 0.0 0.0 0.0 2.1222364893289716 0.0 84-85 0.0 0.0 0.0 2.418405404896304 0.0 86-87 0.0 0.0 0.0 2.813074913821792 0.0 88-89 0.0 0.0 0.0 3.266138400005023 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14720 0.0 26.565527 1 GGTATCA 10680 0.0 24.913984 1 TATAGAC 215 1.6661943E-9 24.298311 3 ATATAGG 425 0.0 23.469473 2 GAGTACT 9210 0.0 23.2562 12-13 GATATAG 475 0.0 23.007034 1 GTACATG 7885 0.0 22.717789 1 AGTACTT 9570 0.0 22.505424 12-13 TATCAAC 17675 0.0 22.116346 2 TCAACGC 17690 0.0 22.068144 4 ATCAACG 17740 0.0 22.005945 3 CAACGCA 17840 0.0 21.88689 5 GTACTTT 9850 0.0 21.601337 14-15 CTTAACG 55 0.0028758342 21.59412 20-21 AACGCAG 18155 0.0 21.559467 6 TACATGG 8570 0.0 20.83915 2 GGGCGTA 390 0.0 20.705769 9 ACATGGG 8310 0.0 20.459917 3 TACTTTT 10570 0.0 20.107447 14-15 ACGCAGA 19625 0.0 19.847748 7 >>END_MODULE