FastQCFastQC Report
Thu 26 May 2016
SRR937076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266604
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110120.8694114340393683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80620.6365051744665262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.4203365850731562No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23190.18308800540658327No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20280.16011318454702494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18450.1456651013260656No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.13721731496189812No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.134690874180091No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16350.12908533369545652No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14910.11771635017732456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.1063473666591926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127100.028.9480341
GTACATG79050.027.5414811
TACATGG81450.025.9712372
GGTATCA87550.025.4648061
ACATGGG81250.024.8065383
ATCAACG152500.024.0641843
GAGTACT77450.023.96374112-13
TCAACGC153800.023.8308184
TATCAAC154200.023.7372282
CAACGCA154950.023.6223345
AACGCAG157600.023.375026
GTACTTT82000.022.75042214-15
CATGGGG28850.022.4095924
AGTACTT82800.022.18575512-13
AACCGAT1503.0213045E-522.1782597
CATGGGA52100.022.0809884
ACGCAGA171600.021.3806427
CGCAGAG172900.021.110778
CAATTCG2050.020.86167778-79
AATTCGC2050.020.86167778-79
Produced by FastQC (version 0.11.5)