##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1266604 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81454503538596 33.0 31.0 34.0 30.0 34.0 2 31.8813986060363 33.0 31.0 34.0 30.0 34.0 3 31.89070143470256 33.0 31.0 34.0 30.0 34.0 4 35.511429775999446 37.0 35.0 37.0 33.0 37.0 5 35.51753507805123 37.0 35.0 37.0 33.0 37.0 6 35.600994470252736 37.0 35.0 37.0 33.0 37.0 7 35.55090620272792 37.0 35.0 37.0 33.0 37.0 8 35.523447738993404 37.0 35.0 37.0 33.0 37.0 9 37.1356548692409 39.0 37.0 39.0 33.0 39.0 10-11 37.005181177384564 39.0 37.0 39.0 33.0 39.0 12-13 36.92296132019163 39.0 37.0 39.0 33.0 39.0 14-15 38.027092129821156 40.0 38.0 41.0 33.0 41.0 16-17 37.9840510530521 40.0 38.0 41.0 33.0 41.0 18-19 37.99397167544079 40.0 38.0 41.0 33.0 41.0 20-21 37.960616735775346 40.0 38.0 41.0 33.0 41.0 22-23 37.85225019027257 40.0 37.5 41.0 33.0 41.0 24-25 37.79668388857133 40.0 37.0 41.0 32.5 41.0 26-27 37.79608188510379 40.0 37.0 41.0 33.0 41.0 28-29 37.716697168175685 40.0 37.0 41.0 33.0 41.0 30-31 37.51456927342721 40.0 37.0 41.0 32.0 41.0 32-33 37.271660676896644 40.0 36.0 41.0 32.0 41.0 34-35 37.08541027819271 39.0 36.0 41.0 31.0 41.0 36-37 36.88127425777907 39.0 35.0 41.0 31.0 41.0 38-39 36.69850324173933 39.0 35.0 41.0 30.5 41.0 40-41 36.47755533694825 39.0 35.0 41.0 30.0 41.0 42-43 36.21532065270598 39.0 35.0 41.0 30.0 41.0 44-45 35.87587793817168 39.0 35.0 40.0 29.5 41.0 46-47 35.40234319487385 38.5 35.0 40.0 26.5 41.0 48-49 35.06251954044042 38.5 35.0 40.0 25.5 41.0 50-51 34.313501299537975 38.0 34.0 39.5 23.5 40.5 52-53 34.46240142933387 38.0 34.0 40.0 24.0 40.5 54-55 34.82725263776207 38.5 35.0 40.0 24.5 41.0 56-57 34.698142829171545 38.0 34.5 40.0 24.5 41.0 58-59 34.483823278625366 38.0 34.0 40.0 24.0 41.0 60-61 34.19324469210582 38.0 34.0 40.0 23.0 41.0 62-63 33.87252487754657 37.0 34.0 40.0 22.0 41.0 64-65 33.527207003925454 37.0 33.5 40.0 22.0 41.0 66-67 33.15880614619881 36.0 33.0 39.0 20.5 41.0 68-69 32.77486530912582 36.0 33.0 39.0 19.5 41.0 70-71 32.361376562840476 35.0 32.5 39.0 18.0 40.0 72-73 31.97862591622954 35.0 32.0 38.0 15.5 40.0 74-75 31.594827191450523 35.0 32.0 37.0 12.0 39.0 76-77 31.15778214816944 35.0 31.0 36.5 7.5 39.0 78-79 30.71463535564391 35.0 31.0 36.0 7.0 39.0 80-81 30.30975979864267 35.0 31.0 36.0 2.0 37.5 82-83 29.9306590694487 34.0 30.0 35.0 2.0 37.0 84-85 29.58277133184484 34.0 30.0 35.0 2.0 36.5 86-87 29.23531545771212 34.0 29.5 35.0 2.0 36.0 88-89 28.878234238957084 34.0 29.0 35.0 2.0 36.0 90-91 28.52838061461988 34.0 29.0 35.0 2.0 35.5 92-93 28.1762808265251 34.0 29.0 35.0 2.0 35.0 94-95 27.8283709825644 34.0 28.5 35.0 2.0 35.0 96-97 27.356680541037292 34.0 27.0 35.0 2.0 35.0 98-99 26.817603607757437 34.0 26.0 35.0 2.0 35.0 100-101 24.691356572377792 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41696185745277603 1101 2 0.3976186325333444 1101 3 0.47931737767357774 1101 4 0.3614683471980058 1101 5 0.43581606184158517 1101 6 0.4143451217725911 1101 7 0.3695669293131445 1101 8 0.4267807532463266 1101 9 0.4712719017081639 1101 10-11 0.46454197040004175 1101 12-13 0.5300368338191745 1101 14-15 0.6863738134051616 1101 16-17 0.6636096969920757 1101 18-19 0.5422424868682114 1101 20-21 0.5574667744572182 1101 22-23 0.660787880843138 1101 24-25 0.5860305243049666 1101 26-27 0.5816013845349204 1101 28-29 0.6997825169738476 1101 30-31 0.5879376773402427 1101 32-33 0.4370344553888046 1101 34-35 0.5130065151505292 1101 36-37 0.7375922352015252 1101 38-39 0.739132440318123 1101 40-41 0.7669368934703442 1101 42-43 0.8360490526168505 1101 44-45 0.797815267271865 1101 46-47 0.8274948708386134 1101 48-49 0.8873035537991001 1101 50-51 1.0153451328109 1101 52-53 0.936313389806692 1101 54-55 0.9753479680799515 1101 56-57 1.039776312270348 1101 58-59 1.0065251431225875 1101 60-61 0.9554430901044455 1101 62-63 0.9162263706104667 1101 64-65 0.8650068180031951 1101 66-67 0.9163515142778351 1101 68-69 0.7731420399197333 1101 70-71 0.620458708931352 1101 72-73 0.7743154740570937 1101 74-75 0.7947543605002387 1101 76-77 0.7979410920302143 1101 78-79 0.8471398682513964 1101 80-81 1.0222814846423276 1101 82-83 0.857066505299084 1101 84-85 0.7872049126617888 1101 86-87 0.8136215661407995 1101 88-89 0.8007429765053047 1101 90-91 0.9522989926126435 1101 92-93 1.105342191146903 1101 94-95 1.186786389378014 1101 96-97 1.222489725483939 1101 98-99 1.2297962190498701 1101 100-101 1.5209637997304206 1102 1 0.21196354741440615 1102 2 0.18515069209951918 1102 3 0.29126043590766315 1102 4 0.16820930590329652 1102 5 0.27096328732223185 1102 6 0.32053535180619264 1102 7 0.28882950958632136 1102 8 0.05853115585482982 1102 9 0.23604117224120102 1102 10-11 0.27360911158142187 1102 12-13 0.25041926219462596 1102 14-15 -0.004041121879254206 1102 16-17 0.19348324809850226 1102 18-19 0.11310307497485894 1102 20-21 0.1447985238657381 1102 22-23 0.1714411007170895 1102 24-25 0.05560326224162537 1102 26-27 0.26097987629387376 1102 28-29 0.19642098447688028 1102 30-31 0.16673114414741264 1102 32-33 0.1464868084118578 1102 34-35 0.25190843773041394 1102 36-37 0.3822519564572602 1102 38-39 0.1766476002680193 1102 40-41 0.08516909419802943 1102 42-43 0.35600551125255464 1102 44-45 0.44439458611792304 1102 46-47 0.3957027162369471 1102 48-49 0.45441540937697056 1102 50-51 0.46093880638984785 1102 52-53 0.7990755823827911 1102 54-55 0.4853247062551844 1102 56-57 0.7805680095882792 1102 58-59 0.34553790730335265 1102 60-61 0.44435694182522667 1102 62-63 0.35544043904396716 1102 64-65 0.5482588012228646 1102 66-67 0.31561538687461166 1102 68-69 0.44229328154447956 1102 70-71 0.33997279525856783 1102 72-73 0.3722885141986474 1102 74-75 0.5741760953949715 1102 76-77 0.5211333301358643 1102 78-79 0.4405052777191578 1102 80-81 0.537210804482477 1102 82-83 0.39950342996655763 1102 84-85 0.47744886359325633 1102 86-87 0.49665037501608467 1102 88-89 0.2397502731631569 1102 90-91 0.1740224552446108 1102 92-93 0.360124782553946 1102 94-95 0.3580580357982761 1102 96-97 0.0920162379995979 1102 98-99 0.1753819398682097 1102 100-101 0.2563048737507394 1103 1 0.355533685401177 1103 2 0.25016865449907755 1103 3 0.15416860975153313 1103 4 0.19362218720758761 1103 5 0.30422334647754923 1103 6 0.176578038422619 1103 7 0.186724164026991 1103 8 0.23520161669934225 1103 9 0.08304553484047972 1103 10-11 0.25467136853894345 1103 12-13 0.13190392116291605 1103 14-15 0.41830799608018054 1103 16-17 0.44452047617654245 1103 18-19 0.4407068824111633 1103 20-21 0.36083551921539936 1103 22-23 0.3419712348109485 1103 24-25 0.5128177362543269 1103 26-27 0.3820187334705807 1103 28-29 0.6219129934305769 1103 30-31 0.4238796079285265 1103 32-33 0.37301033301970676 1103 34-35 0.5570867499841938 1103 36-37 0.4010532360402195 1103 38-39 0.5563286142867341 1103 40-41 0.43758068914909387 1103 42-43 0.43517441704833715 1103 44-45 0.3230658763217562 1103 46-47 0.475126565395108 1103 48-49 0.661927141003062 1103 50-51 0.6986761364452363 1103 52-53 0.7448001504600725 1103 54-55 0.7523498451517128 1103 56-57 0.6613396734269514 1103 58-59 0.7704094835515178 1103 60-61 0.7488873171103165 1103 62-63 0.8156153238434243 1103 64-65 0.8295491319933674 1103 66-67 0.6766474526140343 1103 68-69 0.7228145508027595 1103 70-71 0.6758892373070893 1103 72-73 0.5382936680744201 1103 74-75 0.4529336008852418 1103 76-77 0.43156463929647515 1103 78-79 0.6340173393964079 1103 80-81 0.5796340146954471 1103 82-83 0.5781526109742288 1103 84-85 0.6865928674523154 1103 86-87 0.7153388871200725 1103 88-89 0.46747287785919056 1103 90-91 0.6434128549873392 1103 92-93 0.6187902122340105 1103 94-95 0.7292211910873441 1103 96-97 0.8462476631012557 1103 98-99 0.8820886428687125 1103 100-101 0.7718384352989354 1104 1 0.05528665092763063 1104 2 0.11211199235766856 1104 3 0.16249211363509275 1104 4 0.16272841203121402 1104 5 0.17058548566256349 1104 6 0.0520058976797344 1104 7 0.08475567761388447 1104 8 0.13673055979239024 1104 9 0.28542018153667215 1104 10-11 0.38292009441970265 1104 12-13 -0.01968344224482621 1104 14-15 -0.1322239069754758 1104 16-17 0.09536228509204392 1104 18-19 0.19047264476845527 1104 20-21 0.3681302691764543 1104 22-23 0.010853236902953256 1104 24-25 0.1776492631944322 1104 26-27 0.14627774132672045 1104 28-29 0.2916757513145356 1104 30-31 0.2832607976999597 1104 32-33 0.19034670050017866 1104 34-35 0.03764836394275761 1104 36-37 0.1251379844433913 1104 38-39 0.2547290104321078 1104 40-41 0.16616046347975555 1104 42-43 0.3929192816244793 1104 44-45 0.14433309640498493 1104 46-47 0.2675764352012564 1104 48-49 0.3598879938283801 1104 50-51 0.14439198003457676 1104 52-53 0.1339762571077472 1104 54-55 0.11425312336594828 1104 56-57 0.20378342300965357 1104 58-59 0.06625273906527696 1104 60-61 0.07342384928043799 1104 62-63 -0.04589848560620169 1104 64-65 0.012541587427016054 1104 66-67 -0.3324956048270451 1104 68-69 -0.21921914380921947 1104 70-71 -0.13640629159149853 1104 72-73 -0.12737855501328355 1104 74-75 -0.1251164959255675 1104 76-77 -0.0674672526315021 1104 78-79 0.07878788665566105 1104 80-81 -0.06101873495403254 1104 82-83 -0.14461183831693347 1104 84-85 -0.09325101121147483 1104 86-87 0.13427930952677158 1104 88-89 0.17252484412007263 1104 90-91 -0.14935582282184612 1104 92-93 0.09351535856298909 1104 94-95 0.1781646179673686 1104 96-97 0.29669499579660297 1104 98-99 0.17422526851133568 1104 100-101 0.3026431890433976 1105 1 0.15782462519630158 1105 2 0.2275460223080259 1105 3 0.16613311452299584 1105 4 0.13269641174517943 1105 5 0.24356043962863083 1105 6 0.07523023934982831 1105 7 0.06978347674505159 1105 8 0.12397633033186395 1105 9 0.20074694795842163 1105 10-11 0.18190853975403343 1105 12-13 0.18640733845489166 1105 14-15 0.2554449585935217 1105 16-17 0.3745317078354873 1105 18-19 0.23967412738878124 1105 20-21 0.2790774061340997 1105 22-23 0.3150570733618281 1105 24-25 0.12903951770694988 1105 26-27 0.33752622920892605 1105 28-29 0.4804020565031095 1105 30-31 0.5270415987201602 1105 32-33 0.317467389847117 1105 34-35 0.15203210517545074 1105 36-37 0.35020010790371003 1105 38-39 0.44993254297764196 1105 40-41 0.4849667395879962 1105 42-43 0.4336290682296564 1105 44-45 0.45520514889260966 1105 46-47 0.6421955006975608 1105 48-49 0.6466469071296572 1105 50-51 0.4355725343486583 1105 52-53 0.5147024907873075 1105 54-55 0.3959119538359417 1105 56-57 0.5082047660682179 1105 58-59 0.5883311487046967 1105 60-61 0.7282003538004105 1105 62-63 0.7246070235678062 1105 64-65 0.6660368886885522 1105 66-67 0.5472822404870215 1105 68-69 0.6207856830495686 1105 70-71 0.43730609913377805 1105 72-73 0.497142781490286 1105 74-75 0.7448193247298143 1105 76-77 0.644388644776889 1105 78-79 0.6263443638830211 1105 80-81 0.3471705375012917 1105 82-83 0.23750077646993617 1105 84-85 0.4841780607802306 1105 86-87 0.6725470631305264 1105 88-89 0.6945037370412734 1105 90-91 0.6074536235195751 1105 92-93 0.6709795891557384 1105 94-95 0.6237849885032674 1105 96-97 0.24367314383218996 1105 98-99 0.3284335742838991 1105 100-101 0.419753807536825 1106 1 0.3061200749084847 1106 2 0.22564513415812115 1106 3 0.3365737475939987 1106 4 0.3636965275652102 1106 5 0.3345380588790974 1106 6 0.30401603313912773 1106 7 0.31266594296584316 1106 8 0.4119114815817042 1106 9 0.5130245914852907 1106 10-11 0.49891255681164637 1106 12-13 0.5510529782231686 1106 14-15 0.4166284953971413 1106 16-17 0.539637094252349 1106 18-19 0.582982706643385 1106 20-21 0.6820310133392837 1106 22-23 0.4195493202901446 1106 24-25 0.42856746926931777 1106 26-27 0.43492269951155293 1106 28-29 0.41867324944292506 1106 30-31 0.607974938073518 1106 32-33 0.5719828989350688 1106 34-35 0.680290557911384 1106 36-37 0.5858899536275004 1106 38-39 0.45516603813185696 1106 40-41 0.5254612191601566 1106 42-43 0.49656875085658214 1106 44-45 0.5515958825045644 1106 46-47 0.7320901699004949 1106 48-49 0.531899025098582 1106 50-51 0.5328149051128008 1106 52-53 0.593195430018433 1106 54-55 0.6369481701720545 1106 56-57 0.586584175490735 1106 58-59 0.6928979915879481 1106 60-61 0.687218055943994 1106 62-63 0.683198089197198 1106 64-65 0.2778078032022435 1106 66-67 0.4082612273300015 1106 68-69 0.6937473976572193 1106 70-71 0.6727908059814922 1106 72-73 0.5975508998858423 1106 74-75 0.8103077024837297 1106 76-77 0.7833818480384345 1106 78-79 0.7995764561097936 1106 80-81 0.7220891526601037 1106 82-83 0.6530987252120468 1106 84-85 0.8595292016075753 1106 86-87 1.0109725519139765 1106 88-89 0.9605593712279443 1106 90-91 1.0976982393602235 1106 92-93 1.107883843938879 1106 94-95 1.2354165263643146 1106 96-97 1.3163135723532342 1106 98-99 1.2124447165639083 1106 100-101 1.583453321943331 1107 1 0.30262271116262696 1107 2 0.31059960406076215 1107 3 0.305973602836044 1107 4 0.23113499828033213 1107 5 0.3870550164444211 1107 6 0.3696048347091505 1107 7 0.3644498130384193 1107 8 0.29185392169740254 1107 9 0.3501675957565098 1107 10-11 0.4106380945760151 1107 12-13 0.46910130621152746 1107 14-15 0.5183388421582507 1107 16-17 0.5255585959588345 1107 18-19 0.623529597065037 1107 20-21 0.5354367590287339 1107 22-23 0.5275663651107578 1107 24-25 0.42355565404455575 1107 26-27 0.5158795181550104 1107 28-29 0.5254393966783653 1107 30-31 0.6229860666992622 1107 32-33 0.49429636571654356 1107 34-35 0.36626519952100267 1107 36-37 0.3489532151444834 1107 38-39 0.5200782535866253 1107 40-41 0.7714310259683614 1107 42-43 0.8876702130264036 1107 44-45 0.9753422671316088 1107 46-47 0.9861067741534058 1107 48-49 1.0857373281691025 1107 50-51 0.9702074452144771 1107 52-53 0.9240792975162648 1107 54-55 0.8706843559428634 1107 56-57 1.063880992246041 1107 58-59 0.900472966770252 1107 60-61 1.1383314906841875 1107 62-63 1.2296082491461604 1107 64-65 1.110211128630155 1107 66-67 1.1024775603762933 1107 68-69 1.0686140114939064 1107 70-71 0.9019375064440922 1107 72-73 0.9558164792466748 1107 74-75 1.0042089867099797 1107 76-77 1.0885425694251367 1107 78-79 1.104854113891676 1107 80-81 1.2535300236109563 1107 82-83 1.2174748927879797 1107 84-85 1.2454790255235402 1107 86-87 1.2690297198646796 1107 88-89 1.0532734828403356 1107 90-91 1.1710001941648436 1107 92-93 1.169585493779099 1107 94-95 1.273385809640768 1107 96-97 1.2440264967214922 1107 98-99 1.4934890050613703 1107 100-101 1.5445986120171113 1108 1 -0.06581094193938597 1108 2 -0.017798471759487455 1108 3 0.07107585272329686 1108 4 0.12657036065081684 1108 5 0.0053550459529603245 1108 6 0.09956825525043911 1108 7 0.046108304539401956 1108 8 -0.014937497440840275 1108 9 -0.14002220542272426 1108 10-11 -0.07146330346082408 1108 12-13 0.012835890781339288 1108 14-15 0.12039803741016186 1108 16-17 0.16484100942363256 1108 18-19 0.20076055431106 1108 20-21 0.22041287381139796 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92-93 -4.474221848646835 2316 94-95 -4.758119595308688 2316 96-97 -4.829118431190885 2316 98-99 -5.267723183002321 2316 100-101 -5.418770040573758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2092.0 3 722.0 4 695.0 5 789.0 6 837.0 7 1117.0 8 1410.0 9 1812.0 10 2398.0 11 3298.0 12 4682.0 13 6115.0 14 7012.0 15 9266.0 16 12253.0 17 16503.0 18 13841.0 19 7704.0 20 7242.0 21 7731.0 22 8016.0 23 8926.0 24 10057.0 25 11355.0 26 13266.0 27 15527.0 28 18991.0 29 22633.0 30 28053.0 31 34898.0 32 44264.0 33 58872.0 34 80540.0 35 117783.0 36 176073.0 37 250546.0 38 221230.0 39 38050.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.575861951854066 18.995520922764154 14.955505410671593 21.473111714710186 2 15.250739606417241 21.224532346033133 40.38622223415631 23.138505813393312 3 18.32406734687431 26.92587737309284 31.012572258900086 23.737483021132768 4 12.202321708990144 17.264866931445127 38.4664176864429 32.06639367312183 5 12.980156001801845 38.11399037435691 33.82820835012684 15.077645273714408 6 29.385912215357568 37.22326210817203 18.228260938300334 15.162564738170067 7 26.784913005833317 34.69820639705743 21.512570388487575 17.004310208621682 8 26.23336475798148 33.29016066081847 20.6220499881071 19.85442459309295 9 27.488772514229908 16.353966834275674 20.989393541943866 35.167867109550556 10-11 24.270589304267784 26.90470076522248 27.57676915722813 21.247940773281606 12-13 26.25602010659307 24.96411935484763 28.086495959205298 20.693364579354 14-15 23.231697971295706 25.508361156451514 27.70649283340734 23.553448038845445 16-17 20.97626002002524 29.206159242121725 27.858464613190986 21.95911612466205 18-19 20.821973326928806 26.85639819525017 31.640083395811725 20.681545082009308 20-21 22.117237287720513 25.836774386713046 31.421116204774208 20.624872120792233 22-23 21.78866386081413 25.862467944490557 31.368631053031876 20.980237141663434 24-25 21.52467589277043 26.13529320265081 31.498745735223405 20.841285169355356 26-27 21.318636896394224 26.21329098258243 31.489093931581113 20.978978189442234 28-29 20.91882235666841 26.1007293650872 31.92621470376329 21.054233574481103 30-31 20.99045821868326 26.296786113293358 31.684122428515593 21.02863323950779 32-33 20.91003695986369 26.543790845544724 31.39294706946417 21.153225125127417 34-35 21.374598800645867 26.28942412841538 31.12166061989001 21.214316451048745 36-37 21.574973371979823 26.162493693363643 30.94606256657005 21.316470368086485 38-39 21.383875224869755 26.294829367427607 31.162043208943235 21.1592521987594 40-41 21.10897795000981 26.381307350088846 31.37033821723265 21.13937648266869 42-43 21.113401215338705 26.711712547485888 30.527006013530343 21.64788022364506 44-45 21.123595771751646 26.895967199770404 30.219295873561524 21.761141154916423 46-47 21.408517535708167 27.01831773103624 29.642456459761547 21.930708273494044 48-49 21.73689404705511 27.1654823938102 29.141875888204645 21.955747670930048 50-51 21.720964439291464 27.442945789864915 28.978300234371208 21.857789536472417 52-53 21.658693456862157 27.701054769200645 28.88025815355778 21.759993620379415 54-55 21.372590745192547 27.50949607345604 28.917532509320388 22.200380672031027 56-57 21.554269507622788 27.883965121158848 28.47678066732807 22.084984703890292 58-59 21.715438687636148 27.215782538082127 28.85150003276646 22.217278741515265 60-61 21.855489976325945 27.22543802131178 29.219435225586455 21.699636776775822 62-63 21.73614203642818 27.29606661550172 29.238637117328807 21.72915423074129 64-65 21.716558435149913 27.41657619959339 29.146911947575155 21.71995341768154 66-67 21.49233953535754 27.71168444934759 28.79562381002395 22.000352205270918 68-69 21.26362127302104 27.71506218263436 29.1758574426229 21.845459101721698 70-71 21.622959302578202 27.66046112947887 28.703078278249812 22.01350128969312 72-73 21.502921527160666 27.614067071362953 28.551379676940403 22.33163172453597 74-75 21.281966042914604 28.130828428438548 28.41588348907394 22.171322039572907 76-77 21.454853862728136 27.789634031981485 28.631478532590133 22.124033572700245 78-79 21.388227489723636 27.676218455208467 28.703539176548166 22.232014878519728 80-81 21.233850838677867 28.461990655985236 28.307797819729892 21.99636068560701 82-83 21.284569661344964 28.05372169894952 28.286262482433084 22.37544615727244 84-85 21.228098781360703 27.7849127504484 28.12156151015414 22.865426958036753 86-87 21.431033863186457 27.77533164029906 28.33209008121747 22.461544415297016 88-89 21.55357636557526 27.49618863304238 28.2248509024843 22.72538409889806 90-91 21.28231477627184 27.414786303250416 28.65773370625968 22.645165214218068 92-93 21.201468829628084 27.603784076673605 28.430810352263197 22.763936741435117 94-95 21.118880511482818 27.352004898788767 28.518981029827415 23.010133559900996 96-97 21.077578242336855 27.429156671030793 28.3015605036695 23.191704582962856 98-99 20.994080223048034 27.551447561201964 28.244918430291566 23.209553785458436 100-101 20.834772027828368 27.575763561588634 28.530635303785118 23.05882910679788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1282.0 1 944.0 2 700.5 3 808.5 4 728.0 5 770.0 6 1262.5 7 2547.5 8 4311.0 9 4998.0 10 4105.5 11 3288.0 12 3289.0 13 3355.5 14 3402.0 15 3422.0 16 3394.5 17 3377.0 18 3397.5 19 3529.5 20 3788.0 21 4196.5 22 4771.0 23 5526.0 24 6282.5 25 7226.0 26 8500.0 27 9697.0 28 10699.5 29 12118.0 30 14332.0 31 16709.5 32 19319.0 33 22088.5 34 24886.0 35 27154.5 36 30997.5 37 35043.5 38 36233.0 39 36330.5 40 36577.5 41 38115.0 42 39586.5 43 41407.5 44 43954.5 45 45436.5 46 44773.0 47 45820.0 48 55080.5 49 53899.5 50 43292.0 51 39511.5 52 37304.0 53 37398.0 54 37337.0 55 34620.0 56 31480.5 57 28280.0 58 25949.5 59 23923.0 60 20928.5 61 17802.0 62 14655.0 63 11334.5 64 8351.5 65 6285.0 66 4537.0 67 3566.0 68 2598.0 69 1530.0 70 992.0 71 635.5 72 436.5 73 332.5 74 234.5 75 170.0 76 131.0 77 58.0 78 24.5 79 19.5 80 13.5 81 7.0 82 7.5 83 6.5 84 6.5 85 6.0 86 3.0 87 1.5 88 4.0 89 3.5 90 1.5 91 1.0 92 1.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03908088084357858 2 0.03079099702827403 3 0.025580212915796888 4 0.050528815636142005 5 0.09742587264843629 6 0.1190585218426596 7 0.06095038386109629 8 0.09024130667517236 9 0.075793223454213 10-11 0.07571427217978152 12-13 0.07496423507268254 14-15 0.06868760875538052 16-17 0.05538431901367752 18-19 0.06576641160141607 20-21 0.0600819198423501 22-23 0.034817512024279094 24-25 0.009868909303933984 26-27 0.0030396240656116675 28-29 0.007224041610479676 30-31 0.005447637935771559 32-33 0.007500371070989828 34-35 0.007026663424400997 36-37 0.006395053228949222 38-39 0.0029606727911801953 40-41 0.007539846708205564 42-43 0.0034343804377690262 44-45 0.0021711600468654764 46-47 0.0013421716653350217 48-49 3.158050977258875E-4 50-51 0.001855354949139589 52-53 0.005605540484634503 54-55 0.012592728271819764 56-57 0.008329359452520281 58-59 0.005131832838045671 60-61 0.0021711600468654764 62-63 0.00884254273632485 64-65 0.0022895869585126843 66-67 0.023448528506147145 68-69 0.04859450941257094 70-71 0.06308206827074603 72-73 0.004539698279809633 74-75 0.014369131946527882 76-77 0.021908978654733447 78-79 0.027553994776583685 80-81 0.0774512002172739 82-83 0.029567252274586214 84-85 0.011882166801936517 86-87 0.034383280014906 88-89 0.05163413347818261 90-91 0.045870690444685155 92-93 0.04437061623048719 94-95 0.013303289741703011 96-97 0.01721137782606087 98-99 0.04018619868561918 100-101 0.07808281041272569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1266604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.80864384685061 #Duplication Level Percentage of deduplicated Percentage of total 1 70.66436792047271 31.66374494811277 2 14.706629122279983 13.179682130559298 3 5.67593025018403 7.629922112401856 4 2.843664008328142 5.096829110771333 5 1.5819123589920159 3.544167374550226 6 0.9719873549227644 2.613206112622591 7 0.6441581406546666 2.0204696893951115 8 0.46121676558105046 1.6533198228094137 9 0.34168384953360226 1.3779350929774867 >10 1.8540486862332177 15.36602935313796 >50 0.16439045766073113 5.096077311640267 >100 0.08505959480321411 6.740162872864741 >500 0.003006262000598801 0.9671928372591929 >1k 0.0014147115296935533 1.1179849042184136 >5k 3.536778824233883E-4 1.0606950122522987 >10k+ 1.7683894121169416E-4 0.8725813144271861 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11012 0.8694114340393683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8062 0.6365051744665262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5324 0.4203365850731562 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2319 0.18308800540658327 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2028 0.16011318454702494 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1845 0.1456651013260656 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.13721731496189812 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 0.134690874180091 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1635 0.12908533369545652 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1491 0.11771635017732456 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1347 0.1063473666591926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.947563721573594E-5 0.0 20-21 0.0 0.0 0.0 2.368538232944156E-4 0.0 22-23 0.0 0.0 0.0 4.342320093730953E-4 0.0 24-25 0.0 0.0 0.0 7.895127443147187E-4 0.0 26-27 0.0 0.0 0.0 0.00126322039090355 0.0 28-29 0.0 0.0 0.0 0.00378966117271065 0.0 30-31 0.0 0.0 0.0 0.007895127443147187 0.0 32-33 0.0 0.0 0.0 0.013105911555624331 0.0 34-35 0.0 0.0 0.0 0.01882987895190604 0.0 36-37 0.0 0.0 0.0 0.023961711789951712 0.0 38-39 0.0 0.0 0.0 0.02838298315811414 0.0 40-41 0.0 0.0 0.0 0.03225159560525626 0.0 42-43 0.0 0.0 0.0 0.038370319373695325 0.0 44-45 0.0 0.0 0.0 0.043936384221114094 0.0 46-47 0.0 0.0 0.0 0.05210784112477143 0.0 48-49 0.0 0.0 0.0 0.06102933513552776 0.0 50-51 0.0 0.0 0.0 0.0712930008116191 0.0 52-53 0.0 0.0 0.0 0.08783329280501245 0.0 54-55 0.0 0.0 0.0 0.12284818301537023 0.0 56-57 0.0 0.0 0.0 0.1897199124588269 0.0 58-59 0.0 0.0 0.0 0.26839485742978864 0.0 60-61 0.0 0.0 0.0 0.3381877840272098 0.0 62-63 0.0 0.0 0.0 0.4243236244319456 0.0 64-65 0.0 0.0 0.0 0.5281840259465468 0.0 66-67 0.0 0.0 0.0 0.6969028994066022 0.0 68-69 0.0 0.0 0.0 0.8920704498012006 0.0 70-71 0.0 0.0 0.0 1.0494598153803398 0.0 72-73 0.0 0.0 0.0 1.2243368882460501 0.0 74-75 0.0 0.0 0.0 1.4097934318855776 0.0 76-77 0.0 0.0 0.0 1.6216591768224324 0.0 78-79 0.0 0.0 0.0 1.7940887601807667 0.0 80-81 0.0 0.0 0.0 1.9547151280115964 0.0 82-83 0.0 0.0 0.0 2.0945773106669487 0.0 84-85 0.0 0.0 0.0 2.3852364274864124 0.0 86-87 0.0 0.0 0.0 2.7828745211605206 0.0 88-89 0.0 0.0 0.0 3.2519635181951108 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12710 0.0 28.948034 1 GTACATG 7905 0.0 27.541481 1 TACATGG 8145 0.0 25.971237 2 GGTATCA 8755 0.0 25.464806 1 ACATGGG 8125 0.0 24.806538 3 ATCAACG 15250 0.0 24.064184 3 GAGTACT 7745 0.0 23.963741 12-13 TCAACGC 15380 0.0 23.830818 4 TATCAAC 15420 0.0 23.737228 2 CAACGCA 15495 0.0 23.622334 5 AACGCAG 15760 0.0 23.37502 6 GTACTTT 8200 0.0 22.750422 14-15 CATGGGG 2885 0.0 22.409592 4 AGTACTT 8280 0.0 22.185755 12-13 AACCGAT 150 3.0213045E-5 22.178259 7 CATGGGA 5210 0.0 22.080988 4 ACGCAGA 17160 0.0 21.380642 7 CGCAGAG 17290 0.0 21.11077 8 CAATTCG 205 0.0 20.861677 78-79 AATTCGC 205 0.0 20.861677 78-79 >>END_MODULE