FastQCFastQC Report
Thu 26 May 2016
SRR937076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266604
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122530.967389965608825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94020.7422998822046986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65960.5207626061499885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24930.19682552715765936No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24650.19461489147357816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.18687766657929392No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22130.17471917031684725No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20790.16413969954303004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.1391910968226849No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17470.13792787643178137No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13290.10492624371942613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC804.481684E-429.7012981
GTATCAA150550.028.2196881
GGTATCA106400.026.8874911
GAGTACT96050.024.0346112-13
GTAGCGG1000.001653066623.7516576
CCCTATA1801.9507934E-723.7507212
AGTACTT99200.023.1995912-13
TATCAAC182600.023.1263872
TCAACGC183850.022.9949864
ATCAACG184550.022.9335063
CAACGCA185650.022.797625
GTACTTT101600.022.627314-15
CGTATCA1050.002195957122.6197342
AACGCAG187700.022.4989076
GTACATG80750.021.3628671
CCTATAC2457.517883E-921.3271773
TGTAGCG1353.795462E-421.1117535
ACGCAGA203700.020.7083667
TACTTTT109650.020.70619614-15
CGCAGAG203700.020.6384078
Produced by FastQC (version 0.11.5)