FastQCFastQC Report
Thu 26 May 2016
SRR937075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750468
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.6226781155225806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.4463881204794875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.3019449197034384No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.10446814521072184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.038.612951
GTATCAA35500.036.27231
AGATCGA753.0728005E-431.6748056
TATCAAC43250.029.5548882
TCAACGC43400.029.2318134
CAACGCA43450.029.1962225
ATCAACG43750.028.9979533
AACGCAG44850.028.1789256
ACGCAGA49350.025.2243127
CGCAGAG49200.025.2063278
GAGTACT34150.023.09370412-13
GTCCTAC3500.023.0788961
GCAGAGT53350.022.1768119
AGAGTAC42100.021.8368310-11
GTAGCCC5450.021.7960453
GTGTAGC5750.021.4852141
TAGGACG1353.7859625E-421.1179454
GTACTTT37100.021.002714-15
GTCCTAA3850.020.9808141
TAGCCCT7950.020.918724
Produced by FastQC (version 0.11.5)