FastQCFastQC Report
Thu 26 May 2016
SRR937075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750468
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.35790999749489655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.2956821609982038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.19840952578924084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12450.165896480596108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2650.028.6774334
GTATAGC2900.024.5723271
GTATCAA45300.024.1202961
AGGACGT2704.0017767E-1122.8689275
GGTATCA33850.021.893691
AGTACTT28800.021.35715912-13
ATCAACG53250.020.3367443
TATCAAC53350.020.301332
TCAACGC53550.020.2228134
AGAGTAC43050.020.19036310-11
GAGTACT28250.020.00758612-13
GGACGTG2851.9626896E-919.9987376
CAACGCA54050.019.9478635
GTAGGAC3850.019.7390143
AACGCAG55700.019.3569476
GACGTGG1759.9357334E-518.9988027
TACATGG27200.018.512332
GTACTTT30700.018.25617614-15
GTACATG27850.018.2520471
GGCGTGC2352.5265672E-618.190348
Produced by FastQC (version 0.11.5)