##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750468 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14178885708651 33.0 31.0 34.0 31.0 34.0 2 32.134394804308776 33.0 31.0 34.0 30.0 34.0 3 32.32561015259811 34.0 31.0 34.0 30.0 34.0 4 36.04915466082498 37.0 35.0 37.0 35.0 37.0 5 35.979551426576485 37.0 35.0 37.0 35.0 37.0 6 36.01432972491832 37.0 35.0 37.0 35.0 37.0 7 35.95764909363224 37.0 35.0 37.0 35.0 37.0 8 35.935906660910256 37.0 35.0 37.0 35.0 37.0 9 37.63348603804559 39.0 37.0 39.0 35.0 39.0 10-11 37.564493089645396 39.0 37.0 39.0 35.0 39.0 12-13 37.486123991962344 39.0 37.0 39.0 35.0 39.0 14-15 38.72528075814025 40.0 38.0 41.0 34.5 41.0 16-17 38.64990779087183 40.0 38.0 41.0 34.0 41.0 18-19 38.701841384309525 40.0 38.0 41.0 34.5 41.0 20-21 38.772459318718454 40.0 38.0 41.0 34.0 41.0 22-23 38.69811637538176 40.0 38.0 41.0 34.0 41.0 24-25 38.6095369822564 40.0 38.0 41.0 34.0 41.0 26-27 38.4616319416684 40.0 38.0 41.0 34.0 41.0 28-29 38.30483911372637 40.0 38.0 41.0 34.0 41.0 30-31 38.18606522863067 40.0 38.0 41.0 34.0 41.0 32-33 38.15604063064647 40.0 38.0 41.0 34.0 41.0 34-35 38.06748922006001 40.0 38.0 41.0 33.5 41.0 36-37 37.92797427738425 40.0 37.0 41.0 33.0 41.0 38-39 37.76800743003032 40.0 37.0 41.0 33.0 41.0 40-41 37.60743229558089 40.0 37.0 41.0 33.0 41.0 42-43 37.410607114493885 40.0 37.0 41.0 33.0 41.0 44-45 37.06157890809469 40.0 37.0 41.0 31.0 41.0 46-47 36.827508967737465 40.0 37.0 41.0 31.0 41.0 48-49 36.534708341994595 40.0 36.0 41.0 30.0 41.0 50-51 36.29330297894114 39.0 36.0 41.0 30.0 41.0 52-53 36.0765802139465 39.0 36.0 41.0 29.5 41.0 54-55 35.85774476726523 39.0 35.0 40.0 29.0 41.0 56-57 35.6056474093499 39.0 35.0 40.0 28.0 41.0 58-59 35.32131016912113 38.5 35.0 40.0 28.0 41.0 60-61 35.03093269799645 38.0 34.5 40.0 27.0 41.0 62-63 34.708972267971454 38.0 34.0 40.0 26.0 41.0 64-65 34.362268611053366 38.0 34.0 40.0 26.0 41.0 66-67 34.0289419402293 37.0 34.0 40.0 25.5 41.0 68-69 33.60575933950548 36.5 33.0 39.0 24.5 40.0 70-71 33.149925779646836 36.0 32.5 39.0 23.0 40.0 72-73 32.8248053214794 36.0 32.0 38.5 22.5 40.0 74-75 32.38305630619826 35.0 32.0 38.0 22.0 40.0 76-77 30.91819238128741 34.0 30.0 36.0 20.5 39.0 78-79 31.640211841144456 35.0 31.0 37.0 21.0 39.0 80-81 31.456861585037604 35.0 31.0 36.0 20.0 39.0 82-83 31.09105384906485 34.5 31.0 36.0 19.0 37.5 84-85 30.676371277656077 34.0 31.0 35.5 18.0 37.0 86-87 30.302023537312717 34.0 31.0 35.0 14.0 36.5 88-89 29.91881199464867 34.0 30.0 35.0 7.5 36.0 90-91 29.506128842269092 34.0 30.0 35.0 4.5 36.0 92-93 29.087899150929818 34.0 29.0 35.0 2.0 35.0 94-95 28.648034293267667 34.0 29.0 35.0 2.0 35.0 96-97 28.05301225368703 34.0 29.0 35.0 2.0 35.0 98-99 27.40711995714674 33.0 28.0 35.0 2.0 35.0 100-101 24.52841027199028 31.0 21.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3480337901979951 1101 2 0.26177577747713343 1101 3 0.34417897206411396 1101 4 0.3542192581227468 1101 5 0.3406824359342835 1101 6 0.3252203383475063 1101 7 0.2836465395914374 1101 8 0.3164381536900933 1101 9 0.4722978634482118 1101 10-11 0.4160951137633191 1101 12-13 0.5153088490216575 1101 14-15 0.6049129723618236 1101 16-17 0.5624749701592293 1101 18-19 0.6531251316463411 1101 20-21 0.6774595767653011 1101 22-23 0.5800615266951823 1101 24-25 0.4704857503046611 1101 26-27 0.6766968104169209 1101 28-29 0.5742933790025049 1101 30-31 0.5618806429609293 1101 32-33 0.6517112311824036 1101 34-35 0.6088777438631183 1101 36-37 0.6075442021558715 1101 38-39 0.7450088409913604 1101 40-41 0.8334490224153015 1101 42-43 0.8625683371203792 1101 44-45 0.9918277707800769 1101 46-47 0.9923986988454345 1101 48-49 0.8199207394805725 1101 50-51 1.0242556826474214 1101 52-53 0.9127738264394978 1101 54-55 0.9553114553891504 1101 56-57 0.8941647448529793 1101 58-59 0.8817494487833741 1101 60-61 1.090616300081912 1101 62-63 1.1501889989018466 1101 64-65 0.9766072668881662 1101 66-67 0.9825337427890233 1101 68-69 1.0163608189544604 1101 70-71 0.8571541028128138 1101 72-73 0.9425884771055948 1101 74-75 1.1164918139473627 1101 76-77 0.8262162611349559 1101 78-79 1.0813037581101064 1101 80-81 1.228286291334161 1101 82-83 1.332647108205098 1101 84-85 1.2162587890328247 1101 86-87 1.0302950271144837 1101 88-89 1.1834332632138782 1101 90-91 1.193973122055457 1101 92-93 1.367090038727195 1101 94-95 1.3168756826566614 1101 96-97 1.3363346249769528 1101 98-99 1.4427383634771296 1101 100-101 1.632518754347121 1102 1 0.16723124488518692 1102 2 0.1233368405103974 1102 3 0.16827255400658458 1102 4 0.2188285844617326 1102 5 0.20155653903395176 1102 6 0.24404187509177433 1102 7 0.22678878885754017 1102 8 0.23703698074323398 1102 9 0.23738864445884644 1102 10-11 0.197476751408459 1102 12-13 0.3118563735702793 1102 14-15 0.32633064788623045 1102 16-17 0.30043471858039084 1102 18-19 0.31042956820322587 1102 20-21 0.22450564905084036 1102 22-23 0.34941909937632687 1102 24-25 0.3313514363646135 1102 26-27 0.24326167406735522 1102 28-29 0.16708768079610792 1102 30-31 0.14307594029609305 1102 32-33 0.20599660508673168 1102 34-35 0.2511363981986108 1102 36-37 0.3815722606814944 1102 38-39 0.31659641122507765 1102 40-41 0.3560397095435732 1102 42-43 0.47839716302102886 1102 44-45 0.5968361683958534 1102 46-47 0.744927695547247 1102 48-49 0.7255611920324228 1102 50-51 0.8121754177567055 1102 52-53 0.9222854050488252 1102 54-55 0.7293647650339068 1102 56-57 0.8533424259714835 1102 58-59 0.7675444640829596 1102 60-61 0.6647752759483225 1102 62-63 0.8320323435485406 1102 64-65 0.940560970579881 1102 66-67 0.9845959175189947 1102 68-69 0.8409312585699666 1102 70-71 0.7404036110634493 1102 72-73 0.8566647458448315 1102 74-75 0.6382249342216753 1102 76-77 0.6548093982692542 1102 78-79 0.7486134650287788 1102 80-81 1.0381052213859654 1102 82-83 1.0328315061213011 1102 84-85 1.0652308742443424 1102 86-87 0.752077548937276 1102 88-89 1.196283169266767 1102 90-91 1.259002199690343 1102 92-93 0.9374296209571398 1102 94-95 1.2439151637526429 1102 96-97 1.3303821592375407 1102 98-99 1.4330532643567757 1102 100-101 1.3309851049647357 1103 1 -0.04015700145869516 1103 2 -0.1675285329647025 1103 3 -0.008793094749321995 1103 4 0.037732589936453564 1103 5 0.07853289917910189 1103 6 0.10338218982639802 1103 7 0.1698680718916208 1103 8 0.2168195985061061 1103 9 0.0964362223121853 1103 10-11 0.18913761225571335 1103 12-13 0.19551122829091128 1103 14-15 0.20487921362999373 1103 16-17 0.3665538617186854 1103 18-19 0.21691326530783073 1103 20-21 0.2840729700372151 1103 22-23 0.203636518688306 1103 24-25 0.2863881327478097 1103 26-27 0.40514748660143596 1103 28-29 0.37211897042951847 1103 30-31 0.40405280095179563 1103 32-33 0.4130276197482985 1103 34-35 0.3323345802948552 1103 36-37 0.39030532017581976 1103 38-39 0.36811424036869766 1103 40-41 0.42869986133420923 1103 42-43 0.349875631026201 1103 44-45 0.39876259334476316 1103 46-47 0.4254857184687779 1103 48-49 0.5323973319838302 1103 50-51 0.49842520341769614 1103 52-53 0.6784572099300377 1103 54-55 0.6351784113614087 1103 56-57 0.9042952497624199 1103 58-59 0.7542222326091874 1103 60-61 0.6253538908626055 1103 62-63 0.6163018684806119 1103 64-65 0.5975212340585188 1103 66-67 0.624192017862093 1103 68-69 0.5482476723809668 1103 70-71 0.5881460613097715 1103 72-73 0.5902973556520621 1103 74-75 0.6648887069898919 1103 76-77 0.7119544953513035 1103 78-79 0.5968587430052388 1103 80-81 0.9449156406562516 1103 82-83 0.649019083264033 1103 84-85 0.6227961530240869 1103 86-87 0.29781710734485145 1103 88-89 0.6144384943746317 1103 90-91 0.9350544690577074 1103 92-93 0.9848286653527332 1103 94-95 0.7383902378637295 1103 96-97 1.2413441899509685 1103 98-99 1.2490909200518594 1103 100-101 1.034548462031129 1104 1 0.2283271784540588 1104 2 0.16937865251391315 1104 3 0.13793207197584678 1104 4 0.16215419150091748 1104 5 0.0974829067005345 1104 6 0.14529263459398578 1104 7 0.2276808382758162 1104 8 0.23120760432268384 1104 9 0.2671026215500092 1104 10-11 0.29245255985637186 1104 12-13 0.2769678797475663 1104 14-15 0.3235338794275151 1104 16-17 0.422819217984042 1104 18-19 0.42309739006338276 1104 20-21 0.23641464380746413 1104 22-23 0.3003977883067179 1104 24-25 0.2916340837080469 1104 26-27 0.26088032519141535 1104 28-29 0.4046271186519519 1104 30-31 0.3682477565753217 1104 32-33 0.3118768400260805 1104 34-35 0.4469547477721605 1104 36-37 0.4607445720436445 1104 38-39 0.3019446570562536 1104 40-41 0.3677376189434014 1104 42-43 0.2170721410798535 1104 44-45 0.26370116828334034 1104 46-47 0.2386527444929527 1104 48-49 0.2649621416914911 1104 50-51 0.007693116971324798 1104 52-53 0.16960498107781063 1104 54-55 0.0824952643925485 1104 56-57 0.011755010079326667 1104 58-59 -0.04793992955297455 1104 60-61 0.03252332374632516 1104 62-63 0.03611182686200465 1104 64-65 0.11046712986156138 1104 66-67 0.28973982912419416 1104 68-69 0.36169098582428205 1104 70-71 0.3168493657273572 1104 72-73 0.30786442179056195 1104 74-75 0.31319939244342976 1104 76-77 0.24262540745079164 1104 78-79 0.4039614858222649 1104 80-81 0.5455760227451982 1104 82-83 0.6534121033713376 1104 84-85 0.7234535265386022 1104 86-87 0.4014715224278369 1104 88-89 0.33554414548028433 1104 90-91 0.5034493945954885 1104 92-93 0.32998987479966146 1104 94-95 0.43186664562585264 1104 96-97 0.5758289715786091 1104 98-99 0.4525878864059685 1104 100-101 0.18725668045363264 1105 1 0.2087344018209265 1105 2 0.07507735570140284 1105 3 0.02364531885501009 1105 4 0.145170795548907 1105 5 0.20850702498923113 1105 6 0.1613828054823685 1105 7 0.2232304686236546 1105 8 0.22495624085158994 1105 9 0.2669874839909312 1105 10-11 0.24497407240031066 1105 12-13 0.21163579878768246 1105 14-15 0.2604130455891962 1105 16-17 0.27187387802524654 1105 18-19 0.186988433573255 1105 20-21 0.2810789269246641 1105 22-23 0.3276859957967204 1105 24-25 0.2345846351563523 1105 26-27 0.5151179933528525 1105 28-29 0.36127843364406687 1105 30-31 0.33507541382898154 1105 32-33 0.3039535579996624 1105 34-35 0.29649237957436014 1105 36-37 0.4072795375023759 1105 38-39 0.38482430800765144 1105 40-41 0.23625537436698352 1105 42-43 0.14726242104592302 1105 44-45 0.15080091376873384 1105 46-47 0.215891261036397 1105 48-49 0.1790295274629159 1105 50-51 0.30458925077329013 1105 52-53 0.372432517134051 1105 54-55 0.39021493473553903 1105 56-57 0.33588360055320976 1105 58-59 0.3248500352997681 1105 60-61 0.4267622038820704 1105 62-63 0.38309825274976816 1105 64-65 0.4294575552645412 1105 66-67 0.4171922591326691 1105 68-69 0.4973260603606988 1105 70-71 0.3212779198420108 1105 72-73 0.39732872914724027 1105 74-75 0.5014559674040413 1105 76-77 0.4482592700129011 1105 78-79 0.3119150672063391 1105 80-81 0.6882962990753434 1105 82-83 0.5526491655456169 1105 84-85 0.6747327654187352 1105 86-87 0.5887051910740588 1105 88-89 0.7739370675503352 1105 90-91 0.7318308029096343 1105 92-93 0.9676277368769846 1105 94-95 1.0164473025159246 1105 96-97 1.2239279898794209 1105 98-99 1.399624003240291 1105 100-101 0.8702078378890938 1106 1 0.3066742211489242 1106 2 0.2172184488319715 1106 3 0.2554643868994617 1106 4 0.25297283623260114 1106 5 0.19890476333978313 1106 6 0.19274856878904956 1106 7 0.2564078261332341 1106 8 0.13338543432659833 1106 9 0.2501225980482502 1106 10-11 0.3990976832406261 1106 12-13 0.34816744888191664 1106 14-15 0.5929502523221473 1106 16-17 0.524327247752943 1106 18-19 0.4074667594327508 1106 20-21 0.41194832966723993 1106 22-23 0.4466825513306176 1106 24-25 0.4880128537824717 1106 26-27 0.5963513396841691 1106 28-29 0.6282326807357705 1106 30-31 0.40563416472259917 1106 32-33 0.4208907452356456 1106 34-35 0.40102055270753567 1106 36-37 0.43959984035544153 1106 38-39 0.6501213119720433 1106 40-41 0.5623057740550266 1106 42-43 0.48917848655577245 1106 44-45 0.497741512975864 1106 46-47 0.23063178875460721 1106 48-49 0.22394569045764712 1106 50-51 0.054552693939598385 1106 52-53 0.2030078404589304 1106 54-55 0.3565542146471543 1106 56-57 0.4302205408274702 1106 58-59 0.4036787543483129 1106 60-61 0.4404385215528279 1106 62-63 0.492273205632074 1106 64-65 0.4589914150162855 1106 66-67 0.6367404166682533 1106 68-69 0.8773343465763404 1106 70-71 0.6807575130050765 1106 72-73 0.7421368463890659 1106 74-75 0.4675677825726936 1106 76-77 0.28259994720793813 1106 78-79 0.5305086851955529 1106 80-81 0.6054585139320352 1106 82-83 0.755523345047795 1106 84-85 0.8266223475335082 1106 86-87 0.7758477464779752 1106 88-89 0.8645208395656709 1106 90-91 0.81293823125824 1106 92-93 1.006747610796527 1106 94-95 1.195527045155817 1106 96-97 1.3112216468625881 1106 98-99 1.475373824517991 1106 100-101 1.430437252329856 1107 1 0.1524498863709809 1107 2 0.09633094242440166 1107 3 0.04799898776623479 1107 4 0.06524274629364868 1107 5 0.1307165905451555 1107 6 0.08092485845772046 1107 7 0.17653457947682227 1107 8 0.2241631965708777 1107 9 0.20653948606726402 1107 10-11 0.16428361921155243 1107 12-13 0.1900199350182632 1107 14-15 0.26774870518574545 1107 16-17 0.2592752882021756 1107 18-19 0.3545460734734789 1107 20-21 0.29003852492330395 1107 22-23 0.34182561484708884 1107 24-25 0.13200255507330638 1107 26-27 0.30988354062549917 1107 28-29 0.2198317766861848 1107 30-31 0.35822689970559196 1107 32-33 0.3412068130859609 1107 34-35 0.402383617156012 1107 36-37 0.1469807760933577 1107 38-39 0.30249910217661835 1107 40-41 0.4334350205250388 1107 42-43 0.31751100438044944 1107 44-45 0.2691952153851034 1107 46-47 0.5372310012062513 1107 48-49 0.6269409372072658 1107 50-51 0.7139875464490331 1107 52-53 0.8607242997534001 1107 54-55 0.654415834480325 1107 56-57 0.2609355089061225 1107 58-59 0.1971482776252671 1107 60-61 0.278324140640386 1107 62-63 0.33934203456166045 1107 64-65 0.6035956603212824 1107 66-67 0.18954168886174472 1107 68-69 0.09826437593464732 1107 70-71 0.4099474799187206 1107 72-73 0.4613310073471766 1107 74-75 0.5430168948332792 1107 76-77 0.6483497491558374 1107 78-79 0.6969831248246088 1107 80-81 0.8161043967783961 1107 82-83 0.514354138654209 1107 84-85 0.8005726714069432 1107 86-87 0.636235579089 1107 88-89 0.8325033876806813 1107 90-91 0.9473748312862007 1107 92-93 1.09220417913615 1107 94-95 0.9778386626589644 1107 96-97 0.9526978340101699 1107 98-99 0.865387921167418 1107 100-101 0.7473905120344071 1108 1 -0.014961859449201143 1108 2 -0.03475758659963191 1108 3 -0.08226500861457708 1108 4 0.09378276409771757 1108 5 0.09940137585047637 1108 6 0.04819650669604414 1108 7 0.07676126514746784 1108 8 -0.02538805336223504 1108 9 0.1599594723493709 1108 10-11 0.12103578756214972 1108 12-13 0.09737676064056444 1108 14-15 0.0074517761442010055 1108 16-17 0.11624441322466339 1108 18-19 0.13508904889317108 1108 20-21 0.057480187414611805 1108 22-23 0.03599332332736793 1108 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-0.9132038178947361 2316 100-101 -0.9876172165796469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 7.0 5 17.0 6 27.0 7 97.0 8 198.0 9 393.0 10 675.0 11 1147.0 12 1790.0 13 2228.0 14 2932.0 15 4089.0 16 5274.0 17 7380.0 18 6476.0 19 4219.0 20 3470.0 21 3417.0 22 3649.0 23 4048.0 24 4682.0 25 5436.0 26 6666.0 27 8155.0 28 10151.0 29 12662.0 30 16020.0 31 19876.0 32 25496.0 33 33683.0 34 45820.0 35 64414.0 36 100140.0 37 167035.0 38 162374.0 39 16320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.75442328093098 21.958744475896516 15.197481701823687 22.089350541348814 2 14.75341461789593 23.651942168032512 39.65873809047905 21.935905123592512 3 18.17992841666511 26.992327311200953 31.098108611587122 23.729635660546816 4 12.350309873718631 19.900675179588177 39.18426792916944 28.564747017523757 5 12.52453485381691 39.72387712652056 35.00385761856347 12.747730401099055 6 28.815488079993496 40.17370656170007 18.168432470175 12.842372888131429 7 25.90956576429641 34.92980380242729 22.24425291951156 16.916377513764743 8 24.38664939744266 36.939083345325855 21.133212875165896 17.54105438206559 9 25.37363351935059 17.735599652483515 21.746030476982366 35.14473635118353 10-11 22.887000436394647 29.0888679389847 28.92510335224377 19.099028272376888 12-13 24.456053550834184 26.62391092450076 28.73400407347716 20.186031451187898 14-15 21.265663559272348 28.828610946769217 27.373052548543043 22.532672945415396 16-17 20.22278098466557 31.50833879659093 28.096201303719816 20.172678915023692 18-19 20.185337682619377 30.384906485020014 30.244061039244848 19.18569479311576 20-21 21.61049133317698 29.41995981067309 30.09922073214933 18.870328124000597 22-23 20.80148653906629 29.705130665131623 29.76389399681266 19.72948879898943 24-25 20.52903665118907 29.682872503379688 30.11755111504658 19.67053973038466 26-27 20.742051565301455 29.78393529718641 29.89820060457341 19.575812532938723 28-29 20.29837281477326 30.348183791577444 29.867330635349802 19.486112758299498 30-31 20.936316410924803 29.817515718995796 29.79859162062247 19.447576249456933 32-33 20.668589931508674 29.872877968179516 29.80605228793007 19.652479812381742 34-35 20.84143127933666 30.178660066160525 29.655772056006587 19.32413659849623 36-37 20.79340120462662 29.91078567240552 29.82763445445339 19.468178668514472 38-39 20.621795281801997 30.160928821942925 29.726193141236795 19.491082755018283 40-41 21.15536194842567 30.07276480265021 29.434865140922255 19.337008108001864 42-43 20.86921180624958 30.206609367712705 29.181091345192883 19.743087480844824 44-45 21.049829805407185 30.279047176781848 28.92035532660106 19.750767691209912 46-47 21.053024316858703 30.06574572899275 28.771418330186403 20.109811623962145 48-49 20.972649642122125 30.173672207449908 28.77227534110495 20.081402809323013 50-51 20.931033609739004 30.560704201517186 28.515710884022884 19.992551304720926 52-53 21.155291421261627 30.618887082589346 28.15160035178424 20.07422114436479 54-55 21.101963909269468 30.445524104461104 28.240423643666002 20.212088342603426 56-57 21.16602062449115 30.634524902494046 27.88203878385059 20.317415689164214 58-59 21.17413241602989 30.730065073378277 27.950838185354193 20.144964325237638 60-61 21.127189408622318 30.509840970765246 28.273477782611856 20.089491838000583 62-63 20.990102176242026 30.29069860407106 28.397679847774988 20.32151937191193 64-65 21.02467000503687 30.46507103586305 28.25984121865614 20.250417740443936 66-67 21.13543966623449 30.247699099360798 28.079382571119048 20.53747866328566 68-69 21.125417739330658 30.345331180010337 28.078345778900633 20.450905301758368 70-71 21.278417305491114 30.111983604875363 28.079848947388758 20.52975014224476 72-73 21.11708960275455 30.04511851271473 28.182947174296576 20.654844710234148 74-75 20.79943501883826 30.4232361827794 27.916665278642977 20.86066351973936 76-77 20.648174921814725 30.245742283742928 28.098795273838345 21.007287520604002 78-79 20.87863839630737 30.241662535910923 27.789259502070706 21.090439565710998 80-81 20.723046180846833 30.16710231827065 27.774072388572357 21.335779112310163 82-83 20.775918012187304 30.085694906942873 27.90041481611773 21.237972264752088 84-85 20.91432241678332 30.13596771474697 27.657007164506474 21.292702703963233 86-87 20.620416071466643 30.056113148412685 27.61243950280366 21.711031277317016 88-89 20.562139343557803 30.010413689304283 27.744586780411073 21.682860186726842 90-91 20.45676830990795 29.99601581946199 27.794856009849855 21.752359860780206 92-93 20.305595974778402 30.135861888848027 27.747152443541896 21.811389692831675 94-95 20.063413763145128 30.567259363490518 27.634289536662454 21.735037336701897 96-97 19.952601993328035 30.622017220254154 27.626361926488453 21.79901885992936 98-99 19.988460072650906 30.750908136315058 27.291928180609194 21.968703610424846 100-101 19.745409651807126 30.913285909118464 26.823663603880547 22.51764083519386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 423.0 1 257.5 2 103.5 3 102.0 4 80.0 5 89.5 6 149.5 7 247.0 8 327.5 9 339.0 10 344.0 11 391.5 12 445.5 13 505.0 14 568.5 15 626.0 16 712.0 17 817.0 18 968.0 19 1147.5 20 1375.5 21 1760.0 22 2288.0 23 2935.5 24 3791.5 25 4837.5 26 6381.0 27 8484.5 28 10056.5 29 11650.0 30 13513.0 31 15385.0 32 17774.5 33 20315.0 34 23060.0 35 25721.5 36 28382.0 37 30273.5 38 30335.0 39 29697.0 40 29749.0 41 29981.0 42 29705.0 43 29696.5 44 29705.5 45 29257.5 46 28643.0 47 28177.5 48 28348.0 49 27214.0 50 24414.0 51 21945.5 52 19515.0 53 17102.0 54 15089.0 55 13052.0 56 10873.0 57 9021.0 58 7582.5 59 6562.0 60 5442.0 61 4091.0 62 2898.5 63 1905.0 64 1242.5 65 812.0 66 589.0 67 440.0 68 308.5 69 217.5 70 147.5 71 106.0 72 66.0 73 37.5 74 24.5 75 20.5 76 16.0 77 8.0 78 4.0 79 2.5 80 2.0 81 2.5 82 1.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015990022226130895 2 0.0023985033339196343 3 0.0018655025930486044 4 1.3325018521775746E-4 5 6.662509260887873E-4 6 9.327512965243022E-4 7 0.0 8 0.0 9 0.0 10-11 6.662509260887873E-5 12-13 4.663756482621511E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0026650037043551493 22-23 0.0 24-25 0.0037976302787060875 26-27 0.002864878982181785 28-29 0.004130755741750481 30-31 0.013591518892211258 32-33 0.0010660014817420597 34-35 0.0027316287969640276 36-37 0.0037310051860972087 38-39 0.0015990022226130894 40-41 0.004863631760448147 42-43 0.0023985033339196343 44-45 4.663756482621511E-4 46-47 1.3325018521775746E-4 48-49 3.3312546304439366E-4 50-51 1.3325018521775746E-4 52-53 0.0010660014817420597 54-55 0.0034645048156616938 56-57 1.3325018521775746E-4 58-59 9.99376389133181E-4 60-61 0.0011326265743509382 62-63 0.0 64-65 2.665003704355149E-4 66-67 1.3325018521775746E-4 68-69 0.0 70-71 1.3325018521775746E-4 72-73 0.0 74-75 6.662509260887873E-5 76-77 9.327512965243022E-4 78-79 0.0 80-81 0.007928386020456568 82-83 0.001998752778266362 84-85 0.004330631019577117 86-87 0.0026650037043551493 88-89 0.0017988775004397258 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.001932127685657483 98-99 0.003997505556532724 100-101 9.99376389133181E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 750468.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.78978236442917 #Duplication Level Percentage of deduplicated Percentage of total 1 85.16017359994729 65.39431196856958 2 10.483138774787136 16.099958900240257 3 2.5143154173613467 5.7922120108412 4 0.8721856375389528 2.6789978115198836 5 0.36847800320400054 1.4147672836057317 6 0.17932758040864746 0.8262315522911827 7 0.10514720694618668 0.5651961796337689 8 0.06015622253800532 0.3695506589247678 9 0.038845014721008174 0.2684610203732338 >10 0.1852097228199963 2.603980654507124 >50 0.020161814588863985 1.0934829383706446 >100 0.011644423583946302 1.6531519156884504 >500 5.213920948682845E-4 0.22020672020308155 >1k 6.951894598243793E-4 1.019490385231115 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2686 0.35790999749489655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2219 0.2956821609982038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1489 0.19840952578924084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1245 0.165896480596108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 6.662509260887873E-5 0.0 0.0 0.0 12-13 0.0 1.3325018521775746E-4 0.0 0.0 0.0 14-15 0.0 2.665003704355149E-4 0.0 0.0 0.0 16-17 0.0 2.665003704355149E-4 0.0 0.0 0.0 18-19 0.0 2.665003704355149E-4 0.0 0.0 0.0 20-21 0.0 2.665003704355149E-4 0.0 6.662509260887873E-5 0.0 22-23 0.0 2.665003704355149E-4 0.0 4.663756482621511E-4 0.0 24-25 0.0 2.665003704355149E-4 0.0 7.32876018697666E-4 0.0 26-27 0.0 2.665003704355149E-4 0.0 0.0015990022226130894 0.0 28-29 0.0 2.665003704355149E-4 0.0 0.0037310051860972087 0.0 30-31 0.0 2.665003704355149E-4 0.0 0.008528011853936477 0.0 32-33 0.0 2.665003704355149E-4 0.0 0.01465752037395332 0.0 34-35 0.0 2.665003704355149E-4 0.0 0.0187882761157038 0.0 36-37 0.0 3.331254630443936E-4 0.0 0.024784534450502885 0.0 38-39 0.0 3.9975055565327235E-4 0.0 0.03544454926792348 0.0 40-41 0.0 3.9975055565327235E-4 0.0 0.05536545195797822 0.0 42-43 0.0 5.330007408710298E-4 0.0 0.09454100641199892 0.0 44-45 0.0 5.330007408710298E-4 0.0 0.13531556308863268 0.0 46-47 0.0 5.330007408710298E-4 0.0 0.2269916905184498 0.0 48-49 0.0 5.996258334799085E-4 0.0 0.3124716843356412 0.0 50-51 0.0 6.662509260887872E-4 0.0 0.36470575694100216 0.0 52-53 0.0 6.662509260887872E-4 0.0 0.4320637255685785 0.0 54-55 0.0 6.662509260887872E-4 0.0 0.5028861990118166 0.0 56-57 0.0 6.662509260887872E-4 0.0 0.6515934057148339 0.0 58-59 0.0 6.662509260887872E-4 0.0 0.8187557630705107 0.0 60-61 0.0 6.662509260887872E-4 0.0 0.9811877388509571 0.0 62-63 0.0 6.662509260887872E-4 0.0 1.1666053715814666 0.0 64-65 0.0 6.662509260887872E-4 0.0 1.3971948171007957 0.0 66-67 0.0 6.662509260887872E-4 0.0 1.6955419818033546 0.0 68-69 0.0 6.662509260887872E-4 0.0 2.0292004455886197 0.0 70-71 0.0 6.662509260887872E-4 0.0 2.298299194635881 0.0 72-73 0.0 6.662509260887872E-4 0.0 2.550075419604833 0.0 74-75 0.0 6.662509260887872E-4 0.0 2.8258366779129824 0.0 76-77 0.0 6.662509260887872E-4 0.0 3.163959022903042 0.0 78-79 0.0 6.662509260887872E-4 0.0 3.517804889748797 0.0 80-81 0.0 6.662509260887872E-4 0.0 3.940341227074306 0.0 82-83 0.0 6.662509260887872E-4 0.0 4.356414930416753 0.0 84-85 0.0 6.662509260887872E-4 0.0 4.992551314646327 0.0 86-87 0.0 6.662509260887872E-4 0.0 5.67332651092385 0.0 88-89 0.0 6.662509260887872E-4 0.0 6.394942888970616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 265 0.0 28.677433 4 GTATAGC 290 0.0 24.572327 1 GTATCAA 4530 0.0 24.120296 1 AGGACGT 270 4.0017767E-11 22.868927 5 GGTATCA 3385 0.0 21.89369 1 AGTACTT 2880 0.0 21.357159 12-13 ATCAACG 5325 0.0 20.336744 3 TATCAAC 5335 0.0 20.30133 2 TCAACGC 5355 0.0 20.222813 4 AGAGTAC 4305 0.0 20.190363 10-11 GAGTACT 2825 0.0 20.007586 12-13 GGACGTG 285 1.9626896E-9 19.998737 6 CAACGCA 5405 0.0 19.947863 5 GTAGGAC 385 0.0 19.739014 3 AACGCAG 5570 0.0 19.356947 6 GACGTGG 175 9.9357334E-5 18.998802 7 TACATGG 2720 0.0 18.51233 2 GTACTTT 3070 0.0 18.256176 14-15 GTACATG 2785 0.0 18.252047 1 GGCGTGC 235 2.5265672E-6 18.19034 8 >>END_MODULE