FastQCFastQC Report
Thu 26 May 2016
SRR937074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574973
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.7205555739139056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.5069803277719127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.3542775052045922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.12070131988806432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.042.0790061
GTATCAA27300.035.8523371
CAACGCA32200.029.9538825
TCAACGC32200.029.9538824
ATCAACG32250.029.907443
TATCAAC33000.029.3717042
AACGCAG33350.028.918476
CCCCTAG856.392974E-427.9488531
ACGCAGA36000.026.6577827
CGCAGAG35900.026.3350268
GCAGAGT39250.023.4821139
GAGTACT28300.023.2508312-13
AGAGTAC33050.021.77791610-11
GTACATG12700.021.3247551
AGTACTT28950.021.16977712-13
TACATGG12150.020.7258592
CAGAGTA39200.020.66395610-11
GTACTTT31800.020.61707914-15
GCCGACC1656.3065665E-520.1552988
ACATGGG12600.019.985653
Produced by FastQC (version 0.11.5)