##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937074_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 574973 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06946413135921 33.0 31.0 34.0 30.0 34.0 2 32.19671880244812 33.0 31.0 34.0 30.0 34.0 3 32.21224474888386 34.0 31.0 34.0 30.0 34.0 4 35.76193838667207 37.0 35.0 37.0 35.0 37.0 5 35.78005749835209 37.0 35.0 37.0 35.0 37.0 6 35.84455791837182 37.0 35.0 37.0 35.0 37.0 7 35.79622521405353 37.0 35.0 37.0 35.0 37.0 8 35.75105787576112 37.0 35.0 37.0 35.0 37.0 9 37.45364912787209 39.0 37.0 39.0 35.0 39.0 10-11 37.30884319785451 39.0 37.0 39.0 34.0 39.0 12-13 37.27906006021152 39.0 37.0 39.0 34.0 39.0 14-15 38.52166797397443 40.0 38.0 41.0 34.0 41.0 16-17 38.46185299135786 40.0 38.0 41.0 34.0 41.0 18-19 38.475515372026166 40.0 38.0 41.0 34.0 41.0 20-21 38.42229722091298 40.0 38.0 41.0 34.0 41.0 22-23 38.33482615705433 40.0 38.0 41.0 34.0 41.0 24-25 38.21212909127907 40.0 38.0 41.0 34.0 41.0 26-27 38.262717553693825 40.0 38.0 41.0 34.0 41.0 28-29 38.20377043791622 40.0 38.0 41.0 34.0 41.0 30-31 38.02924398189133 40.0 38.0 41.0 34.0 41.0 32-33 37.79163021567969 40.0 37.0 41.0 33.0 41.0 34-35 37.61421492835316 40.0 37.0 41.0 33.0 41.0 36-37 37.453285632542745 40.0 37.0 41.0 33.0 41.0 38-39 37.291904141585775 40.0 37.0 41.0 32.5 41.0 40-41 37.10455534433791 40.0 36.5 41.0 31.5 41.0 42-43 36.865171929812355 40.0 36.0 41.0 31.0 41.0 44-45 36.5376217665873 40.0 36.0 41.0 30.5 41.0 46-47 36.09860636934256 39.5 36.0 41.0 29.5 41.0 48-49 35.70161642372772 39.0 35.5 41.0 27.5 41.0 50-51 35.03451727298499 38.5 35.0 40.0 26.5 40.5 52-53 35.246365481509564 39.0 35.5 40.0 27.0 40.5 54-55 35.63360018644354 39.5 36.0 41.0 27.0 41.0 56-57 35.561392447993214 40.0 36.0 41.0 26.5 41.0 58-59 35.38194836974954 39.5 35.5 41.0 26.0 41.0 60-61 35.16085624890212 39.0 35.0 41.0 25.5 41.0 62-63 34.917285681240685 39.0 35.0 41.0 23.5 41.0 64-65 34.675610854770575 39.0 35.0 41.0 23.0 41.0 66-67 34.37035739069486 38.0 34.5 40.0 22.0 41.0 68-69 34.04428816657478 38.0 34.0 40.0 22.0 41.0 70-71 33.678712739554726 37.0 34.0 40.0 20.5 41.0 72-73 33.31579830705094 37.0 34.0 39.0 19.0 41.0 74-75 32.9082113420978 36.0 33.5 39.0 18.0 41.0 76-77 32.47897379529126 36.0 33.0 39.0 14.0 40.0 78-79 32.029391814920004 35.0 32.5 37.5 10.0 39.5 80-81 31.582078636735986 35.0 32.0 37.0 7.5 39.0 82-83 31.16001360063864 35.0 32.0 37.0 6.0 39.0 84-85 30.754729352508726 35.0 31.0 36.0 2.0 38.0 86-87 30.396184690411552 35.0 31.0 36.0 2.0 37.0 88-89 30.026041222805244 35.0 31.0 35.5 2.0 37.0 90-91 29.67280637525588 34.0 31.0 35.0 2.0 36.0 92-93 29.33497312047696 34.0 30.5 35.0 2.0 36.0 94-95 28.978041577604515 34.0 30.0 35.0 2.0 36.0 96-97 28.571885114605383 34.0 29.5 35.0 2.0 35.5 98-99 28.067411861078696 34.0 29.0 35.0 2.0 35.0 100-101 26.1736377186407 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5382895885341128 1101 2 0.4347346216434076 1101 3 0.40326624464668015 1101 4 0.38011302526621193 1101 5 0.3406611073733359 1101 6 0.1375069103983222 1101 7 0.22949541363065862 1101 8 0.31589453332614426 1101 9 0.2675537106360153 1101 10-11 0.407803547882601 1101 12-13 0.3691380948790055 1101 14-15 0.6569343012122957 1101 16-17 0.6363216363596038 1101 18-19 0.48943663699958506 1101 20-21 0.6502905609898306 1101 22-23 0.6318833260986025 1101 24-25 0.5875374349757934 1101 26-27 0.5464390643212838 1101 28-29 0.6241572124583357 1101 30-31 0.5861576633965271 1101 32-33 0.694023120207234 1101 34-35 0.6720558741979161 1101 36-37 0.6543146041581522 1101 38-39 0.7078717229268534 1101 40-41 0.7385435848442796 1101 42-43 0.7376998522153784 1101 44-45 1.0405990164681072 1101 46-47 1.1550863097265847 1101 48-49 1.2419772046094053 1101 50-51 1.386845670104158 1101 52-53 1.3773632853735194 1101 54-55 1.2943779635263084 1101 56-57 1.6171939319997435 1101 58-59 1.3499820753978042 1101 60-61 1.4924482034318487 1101 62-63 1.5267202308018355 1101 64-65 1.4873436566944491 1101 66-67 1.646588949642215 1101 68-69 1.4062084002840223 1101 70-71 1.4845777686707393 1101 72-73 1.4386357875697584 1101 74-75 1.2750881364092166 1101 76-77 1.4240041093403946 1101 78-79 1.3795238407276038 1101 80-81 1.1492447325355464 1101 82-83 1.1223989432931916 1101 84-85 1.3437110691643284 1101 86-87 1.2299315819115861 1101 88-89 1.1471073134757859 1101 90-91 1.5168647583255712 1101 92-93 1.5564889312148473 1101 94-95 1.514782144830054 1101 96-97 1.5927612666053932 1101 98-99 1.6895696224959948 1101 100-101 1.9922439255893316 1102 1 0.4367501700921892 1102 2 0.37544714259228584 1102 3 0.40977119462854006 1102 4 0.3968116551665162 1102 5 0.31566931414954524 1102 6 0.2052171334498638 1102 7 0.2811030778957644 1102 8 0.3189267211662923 1102 9 0.23045044347521326 1102 10-11 0.4395335363067332 1102 12-13 0.4498495791824695 1102 14-15 0.4429577293986853 1102 16-17 0.5039981684351389 1102 18-19 0.441297415520296 1102 20-21 0.5084472326669456 1102 22-23 0.5787638872693179 1102 24-25 0.45679485130987274 1102 26-27 0.6446525787220097 1102 28-29 0.6399444582642388 1102 30-31 0.4529271307335634 1102 32-33 0.4871432501334567 1102 34-35 0.6499710074932423 1102 36-37 0.6227703480376263 1102 38-39 0.6166123715213345 1102 40-41 0.6300672808307297 1102 42-43 0.6609319398464706 1102 44-45 0.7418032528923675 1102 46-47 0.8133518291723263 1102 48-49 1.0503403414984334 1102 50-51 1.1107265249910796 1102 52-53 1.0761400437833117 1102 54-55 1.1280395679078623 1102 56-57 1.186904016831896 1102 58-59 0.8880053653648048 1102 60-61 0.8376597654837212 1102 62-63 0.9181575396703323 1102 64-65 1.014499447222967 1102 66-67 0.6871390628093366 1102 68-69 0.6227679469244265 1102 70-71 0.687444957115595 1102 72-73 0.5514746410399383 1102 74-75 0.5768556168425363 1102 76-77 0.7668357062926603 1102 78-79 0.6220859098718456 1102 80-81 0.5862339687006504 1102 82-83 0.6715661714761296 1102 84-85 0.8552610268346399 1102 86-87 0.5932326064628057 1102 88-89 0.4684630729049388 1102 90-91 0.7175290752626289 1102 92-93 0.8242717194454663 1102 94-95 0.6419914953717019 1102 96-97 0.653888791268912 1102 98-99 0.48719915997936525 1102 100-101 0.776422988807429 1103 1 0.27324331435402627 1103 2 0.2262056925844078 1103 3 0.20602401168128637 1103 4 0.15606382750061698 1103 5 0.1523934538638656 1103 6 0.026022123871022984 1103 7 0.032239380152738306 1103 8 0.05031003915739518 1103 9 0.1357917248908791 1103 10-11 0.14985604503226568 1103 12-13 0.04765834023088189 1103 14-15 0.18951147261678614 1103 16-17 0.3025132469218761 1103 18-19 0.26620113868430906 1103 20-21 0.2930181155177749 1103 22-23 0.25334588318494866 1103 24-25 0.4451134917234967 1103 26-27 0.3289185705601696 1103 28-29 0.33453169467372135 1103 30-31 0.28145667803993746 1103 32-33 0.4419652514734622 1103 34-35 0.3648973360331311 1103 36-37 0.337288699534561 1103 38-39 0.5148163531474239 1103 40-41 0.5405858483831025 1103 42-43 0.5688044027751502 1103 44-45 0.5709028188277756 1103 46-47 0.6237660465401902 1103 48-49 0.6868425646860103 1103 50-51 0.7128891480881023 1103 52-53 0.7751460737989291 1103 54-55 0.7188891599882794 1103 56-57 0.9754897385547778 1103 58-59 0.9779505125640782 1103 60-61 0.9982688349857654 1103 62-63 0.9835033950258065 1103 64-65 0.8217274213468784 1103 66-67 0.894587344536447 1103 68-69 0.9359362966400511 1103 70-71 0.5663866761584657 1103 72-73 0.7180505184491537 1103 74-75 0.5162733244252777 1103 76-77 0.8480317570748852 1103 78-79 0.8177723858351023 1103 80-81 0.7433631664109264 1103 82-83 0.7127546143484267 1103 84-85 0.6653436910281592 1103 86-87 0.7255292184145787 1103 88-89 0.7868104326039322 1103 90-91 0.8233778571351174 1103 92-93 0.7231014574092818 1103 94-95 0.7329772713382923 1103 96-97 1.0278256088411162 1103 98-99 1.1993828484825713 1103 100-101 1.1561887065539658 1104 1 0.36342135208016657 1104 2 0.3520427271553572 1104 3 0.3799153428405404 1104 4 0.21060254105887566 1104 5 0.1357912278248108 1104 6 0.34031281037178474 1104 7 0.3081895326159838 1104 8 0.3301780808937309 1104 9 0.4254083346833326 1104 10-11 0.4305692552348219 1104 12-13 0.3485716064249047 1104 14-15 0.567941139013584 1104 16-17 0.6863165806839717 1104 18-19 0.5484193086713702 1104 20-21 0.5529251999513889 1104 22-23 0.39750725966702305 1104 24-25 0.4204492513536522 1104 26-27 0.20059672556573815 1104 28-29 0.40504991838232485 1104 30-31 0.5245928053978162 1104 32-33 0.5794008423725785 1104 34-35 0.5929377104193065 1104 36-37 0.790406440505933 1104 38-39 0.7787548230671462 1104 40-41 0.88265435469728 1104 42-43 0.8392235064577207 1104 44-45 0.7912973316641967 1104 46-47 1.1355227409251611 1104 48-49 1.3894847565248796 1104 50-51 1.3196784915503414 1104 52-53 1.0916809587515957 1104 54-55 1.256480619019932 1104 56-57 1.1223349221538186 1104 58-59 1.1003486118808326 1104 60-61 1.2760235772358683 1104 62-63 1.2385770540680525 1104 64-65 1.3131092755723373 1104 66-67 1.1624005751683129 1104 68-69 1.3799599643836729 1104 70-71 1.47930027527471 1104 72-73 1.4632076350400283 1104 74-75 1.1462000058716697 1104 76-77 1.2054895932317535 1104 78-79 1.3004157883002456 1104 80-81 1.3421124381435554 1104 82-83 1.2849041569586888 1104 84-85 1.281738597318121 1104 86-87 1.3561187177449519 1104 88-89 1.203595009225225 1104 90-91 1.2233771004936962 1104 92-93 1.3330369158961517 1104 94-95 1.3917592299802735 1104 96-97 1.7030811644807429 1104 98-99 1.9258516786392725 1104 100-101 1.9197632319506361 1105 1 0.22249447781571519 1105 2 0.16423989203549638 1105 3 0.21898995293204138 1105 4 0.16160819221402534 1105 5 0.18005472221076246 1105 6 0.1086960286675307 1105 7 0.12322181735786586 1105 8 0.17016663014784683 1105 9 0.2746359394629607 1105 10-11 0.3828885614044353 1105 12-13 0.21466785630562413 1105 14-15 0.36893035622786385 1105 16-17 0.1260686932405619 1105 18-19 0.27458002201042575 1105 20-21 0.4687208677525305 1105 22-23 0.2497375044797252 1105 24-25 0.3626844793613273 1105 26-27 0.11598949675227033 1105 28-29 0.27851811605612653 1105 30-31 -0.22467720955176418 1105 32-33 0.025236693809752353 1105 34-35 -0.06717128371749226 1105 36-37 0.1459404375584299 1105 38-39 5.324453409656371E-4 1105 40-41 0.05289725894571973 1105 42-43 0.3343452766582189 1105 44-45 0.1841268321784213 1105 46-47 0.28172968134947496 1105 48-49 0.3112888851549229 1105 50-51 -0.18230312364098467 1105 52-53 -0.1960964302662873 1105 54-55 0.006246832958041182 1105 56-57 0.2210286889190911 1105 58-59 0.097862315666994 1105 60-61 0.03479877152746269 1105 62-63 0.030669677879238577 1105 64-65 0.031606471199850716 1105 66-67 0.10741578981679112 1105 68-69 0.10573580543035632 1105 70-71 -0.009435583584242124 1105 72-73 0.09564081251598822 1105 74-75 0.05154050652372888 1105 76-77 0.22532907073845365 1105 78-79 0.12920699243099065 1105 80-81 0.30664693351554817 1105 82-83 0.3884644907267223 1105 84-85 0.5177946635628068 1105 86-87 0.6317487738324026 1105 88-89 0.328386934328865 1105 90-91 0.24630085761065246 1105 92-93 0.11502107569767261 1105 94-95 0.2556872599218529 1105 96-97 0.11567698495849399 1105 98-99 -0.04357270932895929 1105 100-101 0.17766265623258093 1106 1 -0.03491286645105163 1106 2 0.009109020429463044 1106 3 -0.08325640164306947 1106 4 -0.12059827010427426 1106 5 -0.07930461496404462 1106 6 -0.0020390799280605165 1106 7 0.13493377480612878 1106 8 -0.04816676001206588 1106 9 -0.17068552848116525 1106 10-11 0.07822991865040052 1106 12-13 0.23711151275487907 1106 14-15 -0.01139713533338238 1106 16-17 0.23468701185581864 1106 18-19 0.08611183921070165 1106 20-21 0.2104526008494645 1106 22-23 0.05088710612542968 1106 24-25 0.14254258339942538 1106 26-27 -0.029115746067695625 1106 28-29 0.09670290471808585 1106 30-31 -0.059520709159663454 1106 32-33 0.13597464373417267 1106 34-35 0.3090547496738125 1106 36-37 0.09289424891692732 1106 38-39 0.09141234805386489 1106 40-41 -0.005652583644661036 1106 42-43 0.09395891276354718 1106 44-45 0.09574303684502183 1106 46-47 -0.09426336307753758 1106 48-49 0.06344545373891464 1106 50-51 -0.04704450589063214 1106 52-53 -0.13672544364325745 1106 54-55 -0.01704069815451703 1106 56-57 0.07054481061888396 1106 58-59 -0.015320597717938256 1106 60-61 0.08708774603505276 1106 62-63 0.025521190034446306 1106 64-65 -0.11783321875434893 1106 66-67 0.1455677327174456 1106 68-69 0.08333618957800581 1106 70-71 0.14842296370115804 1106 72-73 0.18161089900603855 1106 74-75 0.06955595198726172 1106 76-77 0.34207643114397257 1106 78-79 0.25414448319667216 1106 80-81 0.28195720662407453 1106 82-83 0.35790884046020466 1106 84-85 0.28903785858025444 1106 86-87 0.36801094892645025 1106 88-89 0.49213129429113067 1106 90-91 0.4859996199278136 1106 92-93 0.4068781113961286 1106 94-95 0.37005216610117486 1106 96-97 0.3237730673343009 1106 98-99 0.4035458991018288 1106 100-101 0.4304713097836732 1107 1 0.08360032299739117 1107 2 -0.024512082688040948 1107 3 -0.010330742170644669 1107 4 0.024293816226659715 1107 5 0.11967106163753272 1107 6 0.15019456173004642 1107 7 -0.1459363862079215 1107 8 0.09250470281767065 1107 9 0.09920827076514627 1107 10-11 0.1495146051868872 1107 12-13 0.2795335751049919 1107 14-15 0.024195533388777335 1107 16-17 0.25015806358585735 1107 18-19 0.41235027703186944 1107 20-21 0.41448480365864526 1107 22-23 0.2781464415142594 1107 24-25 0.3408519359174065 1107 26-27 0.2146715098418568 1107 28-29 0.2711924559342549 1107 30-31 0.13912780060395136 1107 32-33 0.1001678605892593 1107 34-35 0.1508156266864873 1107 36-37 0.0754687607360438 1107 38-39 0.13019617813266393 1107 40-41 0.5238112738407779 1107 42-43 0.2289965969019505 1107 44-45 0.4270020296507795 1107 46-47 0.10621625322941242 1107 48-49 0.34047868429733086 1107 50-51 0.02950910836081988 1107 52-53 -0.13692242891218598 1107 54-55 -0.025858786540950973 1107 56-57 0.20305080582271984 1107 58-59 0.36326967292269785 1107 60-61 0.16580305949857177 1107 62-63 0.2391011831827825 1107 64-65 0.27154328004661465 1107 66-67 0.1553056566640052 1107 68-69 0.30886376751566047 1107 70-71 0.22135204900433791 1107 72-73 0.5138308373410823 1107 74-75 0.39833806915066816 1107 76-77 0.2928849272042626 1107 78-79 0.2648964530493636 1107 80-81 0.09124634674055443 1107 82-83 0.20442477057325803 1107 84-85 0.2563502943459248 1107 86-87 0.09129289479832181 1107 88-89 0.2190120753805438 1107 90-91 0.12613836607375362 1107 92-93 0.07485858416077207 1107 94-95 -0.01972686781455124 1107 96-97 0.07903514338774187 1107 98-99 0.08078296659069295 1107 100-101 0.11175428340477467 1108 1 0.11246658233208251 1108 2 0.22320006938256398 1108 3 0.1709048314885493 1108 4 0.17468078160344191 1108 5 0.28710002100131504 1108 6 0.002558057026327276 1108 7 0.06314517717099477 1108 8 0.04902880329289161 1108 9 0.27765071087765136 1108 10-11 -0.06981620656755894 1108 12-13 0.2348221019210115 1108 14-15 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98-99 -2.8220769407975155 2316 100-101 -3.226281517272536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 983.0 3 277.0 4 280.0 5 258.0 6 328.0 7 364.0 8 495.0 9 633.0 10 801.0 11 1193.0 12 1820.0 13 2527.0 14 2974.0 15 3887.0 16 5335.0 17 7852.0 18 6799.0 19 3864.0 20 3151.0 21 2908.0 22 2935.0 23 3106.0 24 3325.0 25 3872.0 26 4373.0 27 5039.0 28 6161.0 29 7518.0 30 9223.0 31 11672.0 32 14700.0 33 19813.0 34 27494.0 35 40343.0 36 62724.0 37 110986.0 38 150802.0 39 44124.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.980634700264964 20.043285298482406 16.27130557019856 22.70477443105407 2 13.683620851629424 20.656258481151838 43.55815199615869 22.10196867106005 3 17.02611775429758 25.384181832730867 33.01250047837901 24.577199934592542 4 11.05657475328146 17.768432554319837 42.14398062441713 29.03101206798157 5 11.732645613961818 36.55475565254162 37.981529723154665 13.731069010341892 6 27.60634558224685 38.199660306698505 20.47087199548932 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26.899802053219506 34.62146591940887 19.806506152352924 30-31 19.07009023926974 26.5748387590535 34.49269110617899 19.862379895497778 32-33 18.733541589121533 26.562440210528514 34.654412506479005 20.049605693870948 34-35 19.067522437904405 26.650055659917903 34.41626661100675 19.866155291170944 36-37 18.91063131100109 26.544404314548427 34.41168055365922 20.133283820791267 38-39 18.90276673792085 26.870683222683528 34.309846122270784 19.916703917124835 40-41 19.45112151613397 26.663924332447436 33.90159948237717 19.98335466904142 42-43 19.08036680972072 26.662144593296027 33.9175504276403 20.339938169342954 44-45 19.31959691669797 26.87088672760919 33.53186612124501 20.27765023444783 46-47 19.4070385582339 26.802142404317507 33.13700134704671 20.65381769040188 48-49 19.49757072803372 26.837737502967563 33.05923040733678 20.605461361661938 50-51 19.523506806238103 27.21982153246236 32.584109414636565 20.672562246662974 52-53 19.580679862493465 27.318857405238155 32.353864523192705 20.746598209075675 54-55 19.63770132535569 27.058214770236894 32.44955995408216 20.85452395032525 56-57 19.682861620724623 27.20733147674951 32.09947624854102 21.010330653984845 58-59 19.797036866968615 27.429591673450076 32.00039308996542 20.772978369615885 60-61 19.790516082235005 26.998951101864883 32.27057745060351 20.939955365296605 62-63 19.50608178976645 26.760570729832168 32.41055603195614 21.322791448445244 64-65 19.415322668313237 26.80279858939962 32.4819000491362 21.299978693150933 66-67 19.520932772992307 26.752950139853326 32.30394442047842 21.42217266667594 68-69 19.557181450189002 26.531364864324196 32.48443542200489 21.42701826348191 70-71 19.591364238456674 26.1466353910367 32.52430688733555 21.737693483171082 72-73 19.30669129785121 26.259347586717947 32.61709814726874 21.816862968162106 74-75 19.364358426630112 26.29681944376796 32.41378890458331 21.925033225018613 76-77 19.10896445669116 26.52309827989312 32.34028821531953 22.027649048096194 78-79 19.34170053446164 26.431581254871396 32.06447987417882 22.16223833648814 80-81 19.228929161063377 26.371450789911815 32.15900530783162 22.240614741193195 82-83 19.399580896609855 26.179000119172198 32.42588489705523 21.995534087162717 84-85 19.62589835449897 26.025252643856312 32.04930501739087 22.299543984253845 86-87 19.189046262181353 25.970594272632702 32.31350916928472 22.52685029590122 88-89 19.481252571253624 25.90832116382006 32.13725645390096 22.47316981102536 90-91 19.338388973497462 25.95797828898559 32.23957785201685 22.4640548855001 92-93 19.172483020653566 26.12714329454991 32.237533401992984 22.46284028280354 94-95 18.989240952223565 26.422203473859078 31.936280691988557 22.6522748819288 96-97 18.945029785101884 26.590162565415877 31.9964543202316 22.46835332925064 98-99 19.190336447736236 26.450616021875934 31.73271758696562 22.626329943422213 100-101 19.14400069607587 26.452971373879752 31.612459758113637 22.79056817193074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 432.0 1 259.0 2 105.5 3 121.0 4 98.0 5 96.5 6 156.0 7 304.5 8 526.0 9 621.0 10 558.5 11 546.0 12 579.0 13 645.5 14 729.5 15 797.5 16 880.0 17 1005.0 18 1181.5 19 1331.5 20 1497.5 21 1810.0 22 2215.0 23 2873.0 24 3786.5 25 4580.5 26 5854.0 27 7629.0 28 8742.0 29 9716.5 30 11004.0 31 12473.5 32 14254.0 33 16219.0 34 18208.5 35 20317.0 36 22418.0 37 23352.0 38 22938.5 39 22287.5 40 22173.5 41 22153.0 42 22112.0 43 22206.5 44 21987.0 45 21545.0 46 21459.5 47 21522.5 48 21320.5 49 20301.0 50 18148.5 51 15921.5 52 14038.0 53 12237.0 54 10691.5 55 9234.5 56 7795.0 57 6444.0 58 5256.0 59 4283.0 60 3290.5 61 2404.0 62 1691.0 63 1126.5 64 806.5 65 558.5 66 358.5 67 287.0 68 226.5 69 141.0 70 88.5 71 65.5 72 43.5 73 37.0 74 27.0 75 16.5 76 11.0 77 6.0 78 5.0 79 3.5 80 3.0 81 2.5 82 1.5 83 1.5 84 1.5 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03165366025882955 2 0.029392684526056007 3 0.020000939174535153 4 0.03982795713885696 5 0.05565478726827172 6 0.05861144784189866 7 0.05513302363763168 8 0.06069850236445885 9 0.05356773274571154 10-11 0.06069850236445885 12-13 0.059481053892965416 14-15 0.0546982206120983 16-17 0.04600216010143085 18-19 0.03982795713885696 20-21 0.04600216010143085 22-23 0.027479551213709166 24-25 0.014522421052814654 26-27 0.011478799874081045 28-29 0.01234840592514779 30-31 0.009913508982160903 32-33 0.006782927198320617 34-35 0.009217824141307505 36-37 0.006000281752360546 38-39 0.006261163567680569 40-41 0.0064350847778939175 42-43 0.002695778758306912 44-45 8.696060510667456E-4 46-47 0.002000093917453515 48-49 0.0011304878663867693 50-51 0.003913227229800356 52-53 0.0032175423889469587 54-55 0.0057393999370405225 56-57 0.01426153923749463 58-59 0.007130769618747315 60-61 0.01460938165792133 62-63 0.01043527261280095 64-65 0.0070438090136406405 66-67 0.014783302868134679 68-69 0.019131333123468406 70-71 0.014696342263028003 72-73 0.01600075133962812 74-75 0.01826172707240166 76-77 0.021740151276668646 78-79 0.032871108730322986 80-81 0.028175236054562562 82-83 0.030349251182229427 84-85 0.017826924046868288 86-87 0.02069662401538855 88-89 0.018696530097935034 90-91 0.01800084525708164 92-93 0.02730563000349582 94-95 0.018783490703041708 96-97 0.032871108730322986 98-99 0.04826313583420439 100-101 0.05617655089891178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 574973.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.47750741620368 #Duplication Level Percentage of deduplicated Percentage of total 1 88.73729858214688 71.41356604738606 2 8.473721893212044 13.638880330076395 3 1.6529083524658872 3.990658325816353 4 0.5088252675541878 1.637959569725759 5 0.2131703306114252 0.857770843134778 6 0.10909634793170672 0.52678812898528 7 0.05288632588965111 0.29793117788001133 8 0.03691984082322995 0.23769734109252383 9 0.027827046110498785 0.20155061787558368 >10 0.15536637638795708 2.3464107481683927 >50 0.01642200099907683 0.9289462289797509 >100 0.014477244487796378 2.121685702999184 >500 4.321565518617669E-4 0.21858527404511177 >1k 6.482348277926503E-4 1.5815696638347587 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4143 0.7205555739139056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2915 0.5069803277719127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2037 0.3542775052045922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 694 0.12070131988806432 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 8.696060510667457E-5 0.0 1.7392121021334915E-4 0.0 22-23 0.0 1.7392121021334915E-4 0.0 7.826454459600712E-4 0.0 24-25 0.0 1.7392121021334915E-4 0.0 0.001217448471493444 0.0 26-27 0.0 1.7392121021334915E-4 0.0 0.002000093917453515 0.0 28-29 0.0 2.608818153200237E-4 0.0 0.005391557516613824 0.0 30-31 0.0 3.478424204266983E-4 0.0 0.011565760479187719 0.0 32-33 0.0 3.478424204266983E-4 0.0 0.018522608887721684 0.0 34-35 0.0 3.478424204266983E-4 0.0 0.02469681185029558 0.0 36-37 0.0 3.478424204266983E-4 0.0 0.03174062086393622 0.0 38-39 0.0 3.478424204266983E-4 0.0 0.04278461771248389 0.0 40-41 0.0 4.348030255333728E-4 0.0 0.06852495682405957 0.0 42-43 0.0 5.217636306400474E-4 0.0 0.11687505326337062 0.0 44-45 0.0 5.217636306400474E-4 0.0 0.1593118285554278 0.0 46-47 0.0 5.217636306400474E-4 0.0 0.2728823788247448 0.0 48-49 0.0 5.217636306400474E-4 0.0 0.3723653110667805 0.0 50-51 0.0 5.217636306400474E-4 0.0 0.432368128590386 0.0 52-53 0.0 5.217636306400474E-4 0.0 0.5159372700979002 0.0 54-55 0.0 5.217636306400474E-4 0.0 0.5958540661909342 0.0 56-57 0.0 5.217636306400474E-4 0.0 0.7708188036655634 0.0 58-59 0.0 5.217636306400474E-4 0.0 0.9807417043930758 0.0 60-61 0.0 6.956848408533966E-4 0.0 1.181359820374174 0.0 62-63 0.0 6.956848408533966E-4 0.0 1.412501108747715 0.0 64-65 0.0 6.956848408533966E-4 0.0 1.7069497176389152 0.0 66-67 0.0 8.696060510667458E-4 0.0 2.078619343864842 0.0 68-69 0.0 8.696060510667458E-4 0.0 2.476985875858518 0.0 70-71 0.0 8.696060510667458E-4 0.0 2.7990009965685347 0.0 72-73 0.0 8.696060510667458E-4 0.0 3.1033631144418954 0.0 74-75 0.0 8.696060510667458E-4 0.0 3.432682925980872 0.0 76-77 0.0 8.696060510667458E-4 0.0 3.8518330425950436 0.0 78-79 0.0 8.696060510667458E-4 0.0 4.264635035036427 0.0 80-81 0.0 8.696060510667458E-4 0.0 4.763614987138526 0.0 82-83 0.0 8.696060510667458E-4 0.0 5.246594187900997 0.0 84-85 0.0 8.696060510667458E-4 0.0 6.027413461153828 0.0 86-87 0.0 8.696060510667458E-4 0.0 6.787623070996377 0.0 88-89 0.0 8.696060510667458E-4 0.0 7.6155749922170255 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 42.079006 1 GTATCAA 2730 0.0 35.852337 1 CAACGCA 3220 0.0 29.953882 5 TCAACGC 3220 0.0 29.953882 4 ATCAACG 3225 0.0 29.90744 3 TATCAAC 3300 0.0 29.371704 2 AACGCAG 3335 0.0 28.91847 6 CCCCTAG 85 6.392974E-4 27.948853 1 ACGCAGA 3600 0.0 26.657782 7 CGCAGAG 3590 0.0 26.335026 8 GCAGAGT 3925 0.0 23.482113 9 GAGTACT 2830 0.0 23.25083 12-13 AGAGTAC 3305 0.0 21.777916 10-11 GTACATG 1270 0.0 21.324755 1 AGTACTT 2895 0.0 21.169777 12-13 TACATGG 1215 0.0 20.725859 2 CAGAGTA 3920 0.0 20.663956 10-11 GTACTTT 3180 0.0 20.617079 14-15 GCCGACC 165 6.3065665E-5 20.155298 8 ACATGGG 1260 0.0 19.98565 3 >>END_MODULE